research centers


Search results: Found 82

Listing 1 - 10 of 82 << page
of 9
>>
Sort by

Article
Immunohistochemical Expression of Ki 67 &CD31 in Iraqi patients with Astrocytomas, A clinicopathological Studies

Authors: Zainab Khalid Shehab Almukhtar --- Mohammed Subhi Kamal --- Saba Ali Abd Alkareem
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2019 Volume: 12 Issue: 1 Pages: 4077-4086
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

background: Astrocytomas are the most common primary tumor of the central nervous system, the diagnosis absolutely based on histology has a high interobserver variations and remains problematic even for an experienced neurological pathologist.The objective of this study: To assess the immunohistochemical expression of Ki67 as proliferative markers and CD31 as an endothelial cell marker in astrocytomas interrelated with some clinicopathological parameters (age, gender, site of the tumor, and tumor grade) in Iraqi patients.Materials and methods: In this retrospective study, 41 formaline–fixed paraffin –embedded tissue blocks represent cases of Astrocytomas, The histopathologic diagnosis had been revised and all cases were stained by immunohistochemical technique with Ki 67 & CD 31 antibodies and assessed independently by three pathologists. Values were considered statistically significant when p<0.05.Results: Fibrillary astrocytoma (WHO grade II) was found to be the most common type among astrocytic tumors with the peak age incidence in the second and sixth decades of life, and a slight male predominance had been identified. Parietal location of tumor consider most common site ,There was a significant correlation between the age of the patients and the grade of the tumor on one side & Ki-67 labeling indix, and microvessel density (MVD) detected by CD31 on other side (p<0.05). While no significant correlation with the site and gender. Conclusion: A significant correlation was found between Ki67 labeling indices, and MVD (microvessel density) in one side, and the clinicopathological variables of astrocytomas in other side, Could be used as ancillary methods in the differentiation of borderline grades of astrocytomas.


Article
Overexpression of mammaglobin in tissue breast carcinoma by immunohistochemistry

Author: Shoroq Mohammad Abbas شروق محمد عباس
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2012 Volume: 8 Issue: 13 Pages: 59-68
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

Our purpose was to investigate the mammaglobin overexpression in tissue breast cancer and to determine its correlations with conventional prognostic parameters. There were investigated 60 patients with breast carcinoma, and slides from paraffin blocks were stained with an antibody against mammaglobin. The immunohistochemical reaction was scored based on the percentage of positive tumor cells in tumors. Positive reaction for mammaglobin was diffused cytoplasmic staining foundin 75% of tissue breast carcinoma. A significant correlation was found between the mammaglobin expression in the breast cancer and grades of the tumor. Out results suggest that mammaglobin is a good prognostic marker forbreast carcinomaAim of study:-this study was to investigate the mammaglobin overexpression in tissue breast cancer and to determine its correlations with prognostic significant.

يهدف البحث عن التعبير المناعي النسيجي الكيميائي لجين أل mammaglobinفي خلايا سرطان الثدي و معرفة ترابط هذا التغير مع درجة تمايز الورم. تمت دراسة 60 عينة من سرطان الثدي في مختبرات مستشفى الديوانية التعليمي للفترة منذ بداية تشرين الثاني 2005 وحتى آب 2011 , تراوحت أعمارهم بين 45 و 60 سنه, صنفت العينات حسب درجة التمايز إلى 20 عينات من الدرجة الأولى 20 عينة من الدرجة الثانية و20 عينة من الدرجة الثالثة على التوالي . أظهرت الدراسة المناعية النسيجية أن تعبير أل- mammaglobinكان موجبا في 45 عينة (75%)من سرطان الثدي, وكان أكثر في العينات ذات التمايز من (الدرجة الاولى والثانية ) عنه في العينات ذات التمايز من (الدرجة الثالثة ) كما انه توجد علاقة واضحة لتعبير ا لmammaglobin مع درجة التمايز ( قيمة ألفا <0.05).مما يدل علىأن جين أل-mammaglobin يلعب دور أساسي في تقييم حالة سرطان الثدي المستقبلية .


Article
Study of BRCA1 gene expression in breast cancer in relation to some clinicopathological parameters in Al-Diwanyia city by immunohistochemistry

Author: Maather Baqer Hussein Al-Harmooshy ماثر باقر حسين
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2013 Volume: 9 Issue: 15 Pages: 13-26
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

Background: Breast cancer causes major part of cancer deaths in women and is increasing in incidence. The tumor suppressor gene, BRCA1 has been conferred to increase the susceptibility to breast cancer. We aimed to determine the significance of BRCA1 gene expression in relation to other prognostic factors. Materials and methods: 45 patients with positive family history of breast cancer were selected from Al-Diwaniya Teaching Hospital / department of pathology for the study. A control group of 10 healthy subjects were also included. BRCA1 expression was assessed and correlated with age, family history , histological type and grade of breast cancer. Results: BRCA1 was found in 9 patients 'samples (20% of the breast cancer tissues) while remaining patients (80%) were negative as well as the control group. A positive significant relationship was demonstrated between BRCA1 expression and high histological grade, age of the patient and family history and A significant negative correlation was found between BRCA1 expression and type of the tumor. Conclusion: The study demonstrated the lack of BRCA1 gene expression in the majority of breast cancer cases and confirmed the relationship between BRCA1 expression and parameters that determine the poor prognosis in breast cancer .

البحث عن التعبير المناعي النسيجي الكيميائي لجين أل BRCA1 في خلايا سرطان الثدي و معرفة ترابط هذا التغير مع بعض المتغيرات السريرية - المرضية . تمت دراسة(45) عينة مطمورة بالفورمالين من سرطان الثدي و (10) عينات من نسيج الثدي الطبيعي في مختبرات مستشفى الديوانية التعليمي والتي تم استئصالها للفترة منذ بداية تشرين الثاني 2005 وحتى آب 2011 , تراوحت أعمارهم بين 35 و 75 سنه مع معدل عمر(55 ) . مع وجود تاريخ عائلي من الدرجة الأولى للمرض في 25 عينة من العينات , صنفت العينات حسب درجة التمايز إلى( 13 ) عينات من الدرجة الأولى و(32 ) عينة من الدرجة الثالثة, وحسب النوع إلى 30 عينة سرطان الثدي القنوي و15 عينة من سرطان الثدي الفصيصي . أظهرت الدراسة المناعية النسيجية أن تعبير أل BRCA1 كان موجبا في (9 ) عينة (20 %) من سرطان الثدي, وكان أكثر في العينات ضمن مجموعة العمر الأصغر من 50 سنة والعينات ذات التاريخ العائلي المرضي والعينات ذات التمايز الضعيف (الدرجة الثالثة ) عنه في العينات ذات التمايز القوي (الدرجة الأولى ) وفي سرطان الثدي الفصي عنه في سرطان الثدي القنوي كما انه توجد علاقة واضحة لتعبير ا ل BRCA1 مع التاريخ العائلي المرضي للمرضى و عمر المرضى و درجة التمايز ( قيمة ألفا < 0.05).


Article
HIGH EXPRESSION OF P53 PROTEIN IN TOXOPLASMOSIS AMONG WOMEN WITH SPONTANEOUS MISCARRIAGE IN BASRAH

Author: Ghazi Y . AL – Emarah* , Maysoon sharief, Muntaha A. H. Nasir
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2015 Volume: 14 Issue: 1 Pages: 228-235
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

Recurrent abortion is a worldwide problem, with undefined causes. Apoptosis couldplay a major role in the process. The Objective of the work to detect the expressionof p53 protein at the materno-fetal interface in patients with recurrent pregnancy loss(RPL). Immunohistochemistry analysis of P53 protein using paraffin embeddedsections of curate samples obtained from 40 women divided into three groups : 16women with recurrent abortion as postive with toxoplasmosis,10 women withrecurrent abortion negative of toxoplasmosis and14 women with no histry of abortionas control group .The mean value of the expression of P53 protein was (40.87± 7.54),which issignificantly higher than that of the second group(28.2± 4.89),and the third group(13.07± 4.49). The high expression of p53 protein in women with RPL may have arole in accelerating placental apoptosis leading to failure of pregnancy.


Article
Computer Assisted Immunohistochemical Score Prediction Via Simplified Image Acquisition Technique

Authors: Salam N. Jawad --- Bashar H. Abdullah
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2017 Volume: 29 Issue: 1 Pages: 83-88
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: techniques of image analysis have been used extensively to minimize interobserver variation of immunohistochemical scoring, yet; image acquisition procedures are often demanding, expensive and laborious. This study aims to assess the validity of image analysis to predict human observer’s score with a simplified image acquisition technique.Materials and methods: formalin fixed- paraffin embedded tissue sections for ameloblastomas and basal cell carcinomas were immunohistochemically stained with monoclonal antibodies to MMP-2 and MMP-9. The extent of antibody positivity was quantified using Imagej® based application on low power photomicrographs obtained with a conventional camera. Results of the software were employed to predict human visual scoring results with stepwise multiple regression analysis.Results: the overall prediction of epithelial score depicted as r square value was 0.26 (p<0.001) which was obviously higher than that of stromal score (0.10; p<0.01). Epithelial and stromal MMP-2 score prediction was generally higher than that of MMP-9. Collectively, ameloblastomas had a more efficient score prediction compared to basal cell carcinomas.Conclusion: there is a considerable variability in the prediction capacity of the technique with respect to different antibodies, different tumors and cellular versus stromal score


Article
Immunohistochemical expression of p53 and p21 in gliomas: a clinicopathological study
تعبير مناعي كيميائي من p53 و p21 في الاورام الدبقية: دراسة إكلينيكية

Authors: Jalal Ali Jalal --- Tara Muhammad Ali Saeed
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2013 Volume: 17 Issue: 2 Pages: 435-442
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Loading...
Loading...
Abstract

Background and objective: To evaluate p53 and p21 proteins over expression in gliomas and their relation to some clinico-pathological parameters.Methods: From September 2009 to July 2010, a formalin fixed, paraffin embedded blocks of 60 gliomas cases were collected in addition to 44 astrocytomas, 5 oligodendrogliomas, 2 oligoastrocytomas, and 9 ependymomas. These cases were evaluated by immunohistochemistry using streptavidin-biotin method.Results: Overall, 53% of gliomas were positive for p53; these cases formed 66% of astrocytomas, 20% of oligodendrogliomas and 100% of oligoastrocytomas. In contrast to astrocytomas, all ependymoma cases were negative for p53 protein. There was a significant association of p53 expression with patient’s age and tumor site. On the other hand, p21 expression was positive in 25% of gliomas; they comprised 23% of astrocytomas, 40% of oligodendrogliomas, 50% of oligoastrocytomas and 22% of ependymomas. Both p53 and p21 expressions seemed to be raising from low to high grades astrocytoma but they did not reach level of significance.Conclusion: The results of the present study suggest that p53 overexpression is common in gliomas and p21 expression is less common. There was a trend of both marker expressions increasing with higher grades.

Keywords

Glioma --- p53 --- p21 --- Immunohistochemistry


Article
P53 IMMUNOHISTOCHEMISTRY IN CHRONIC PERIODONTITIS; RELATION TO SMOKING AND HISTOPATHOLOGIC PARAMETERS
الاظهار الكيميائي النسيجي المناعي ل p53 في التهاب ما حول الاسنان و علاقته بالتدخين و مؤشرات نسيجية مرضية

Authors: AMEERA K. KHALEEL اميره خليل --- CHINAR M. SULAIMAN جنار سليمان
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2014 Volume: 8 Issue: 2 Pages: 57-77
Publisher: University of Dohuk جامعة دهوك

Loading...
Loading...
Abstract

ABSTRACTBackground and objectives Smoking is one of the main and important risk factors that increase risk of oral health problems. There is a dose-response relationship between number of cigarettes smoked and the development of periodontal diseases, in addition, to an elevation of p53 in oral mucosa of smokers. The aim of the study was to examine p53 immune expression in the gingival tissue samples in patients with different severity of chronic periodontitis in relation to different histopathological parameters and smoking status.Methods Gingival tissue biopsies were taken from (30) smokers and (30) nonsmoker subjects with different severity of chronic periodontitis. Staining profiles were classified according to the number of positive cells as well as to the location of the positive cells in the differentepithelial layers of the specimens.Results No significant difference was found between the median p53 labeling indices andany of the smoking status, severity of chronic periodontitis, epithelial thickness and number of blood vessels in the connective tissue. However, a significant association was found between p53 immunostaining and number of inflammatory cells.Conclusions Chronic periodontitis may accelerate smoking effects on p53 gene mutation.

خلفية واهداف البحث: إلتهاب أنسجة ما حول الأسنان هو أصابة سببها مختلف أنواع الأحياء المجهريه والتدخين هو واحد من أهم عوامل الخطورة. الهدف من الد ا رسة هو إيجاد سمك الطبقة الطلائية للثة، عدد الخلايا الإلتهابية والأوعية الدموية فى نماذج نسيج اللثة للمرضى المدخنين وغير المدخنين الذين عندهم إلتهاب أنسجة ما p والتعبي ا رلمناعي للبروتين 53 حول الأسنان المزمن.طرق البحث: ثلاثون شخص مدخن و ثلاثون شخص غير مدخن عندهم مختلف درجات إلتهاب أنسجة ما حول الأسنانالمزمن أختيروا من المركز الصحي فى دهوك فى الفترة من تشرين الاول ٢٠١٠ الى كانون الثانى ٠٢٠١١ نماذج اللثةباستعمال p جمعت ومررت للتصبيغ بمادة هيماتوكسيلين والإيوسين وللتصبيغ الكيميائي النسيجي المناعي لبروتين ٥٣.(Leica NovoLink MT Polymer UK) النتائج: أظهرت النتائج زيادة واضحة فى سمك الطبقة الطلائية الكبير و سمك الطبقة الطلائية فى القاعدة مع زيادة شدة اكثر من p اقل من ٠,٠٥ )، وفرق غير معنوى وجد فى متوسط عدد الخلايا الإلتهابية (قيمة p حالة التدخين (قيمة ٠,٠٥ ) بين غير المدخنين والمعتدلين وكثيرين التدخين، بالاضافة الى ذلك، كثيري التدخين أظهروا أقل عدد من الأوعية اكثر من ٠,٠٥ ) في متوسط p الدموية فى اللثة مقارنة بمعتدلي التدخين. كثيري التدخين أظهروا زيادة غير معنوية (قيمة مع سمك الطبقة p بين المجاميع. علاقات غير معنوية أيضا وجدت بين متوسط معدل مقياس 53 p معدل مقياس 53 .( اكثر من ٠,٠٥ p الطلائية الكبيرو سمك الطبقة الطلائية فى القاعدة وعدد الأوعية الدموية فى النسيج ال ا ربط للثة (قيمة .( اقل من ٠,٠٥ p وعلى العكس علاقة معنوية وجدت مع عدد الخلايا الإلتهابية (قيمة له دور مهم في إلتهاب أنسجه ما حول الاسنان المزمن. p الاستنتاجات: أشارت نتائج الد ا رسة الحالية أن بروتين p53 له دور مهم في إلتهاب أنسجه ما حول الاسنان المزمن.


Article
Clinicopathological, immunohistochemical charachtaristic and the outcome of Hodghkin lymphoma patients in Erbil city, Iraq

Authors: Lilan Bahzad Mohammedzaki --- Kawa Muhammedamin Hasan --- Ranan Kardagh Polus --- Ahmed Khudair Yassin
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2019 Volume: 8 Issue: 1 Pages: 14-20
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

BACKGROUND: Hodgkin lymphoma (HL) has unique epidemiological features with diversifiedpathologies and exhibits considerable clinicopathological variations in different parts of the world.OBJECTIVES: We aimed in this study to assess clinic-pathological features, immunohistochemistryand outcome of HL patients treated in Erbil, northren Iraq.PATIENTS AND METHODS: This was a retrospective study conducted in Nanakaly Hospital forblood diseases and oncology in Erbil, North Iraq; a total of 125 patients diagnosed between January2012 and December 2016 were involved; they were assessed for their clinical characteristics andhistopathology and immunophenotyping findings and their outcome was evaluated as well.RESULTS: The median age was 28 years (range: 18–71 years); 55% were male and 41% had StageII disease; common histological type is nodular sclerosis (51.2%) followed by mixed cellularity (43.2%);CD30 was positive in nearly all cases of classical HL, and CD15 was positive in 98.7% and CD20was positive in 75% in nodular lymphocyte predominant subtype. Most of the patients receivedadriamycin, bleomycin, vinblastine, and dacarbazine chemotherapy, and the 5‑year overall survivalin our study is 70%. Advanced stage (IV), high lactate dehydrogenase level, low hemoglobin, andsplenomegaly are significant predictors for poor survival.CONCLUSION: Our patient exhibited outcomes that were lower to those reported in developedcountries.


Article
Digital Image Analysis versus Visual Scoring for Bcl-2 and P53 Protein Expression in Glioma

Author: Hayder F. Ghazi
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 2 Pages: 200-204
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Traditionally, evaluation of the results of immunohistochemistry was done by visual quantification. Materials and methods: for reliable evaluation, more time-efficient and user friendly method we used simple computer program with image analysis options as independent parameters for reading positive results. To test the validity of visually scored results, we compare and correlate the results of Digital image analysis (DIA) variables with the visual scores of 280 pictures taken from entire stained glioma tumor sections for Bcl-2 and P53 oncoproteins in different glioma tumor grades.
Results: In this study, rates expression of both oncoproteins was evaluated visually in glioma tumor samples (Bcl-2=72.41% and P53=82.76%), no statistical significant differences were observed according to pathologic grades. Similarly, these results were also explained by data obtained by DIA variables that has been closely correlated with visual scores. More importantly, the DIA variables explained little discrepancy in the visual scores of both oncoproteins. Conclusions: the quantitative DIA measurements of the immunohistochemically stained sections made the results more objective and supported pathomorphological diagnosis.


Article
P53 expression in ovarian tumors: (an immunohistochemical study)

Authors: Shuaib H. Saleem شعيب هاشم سليم --- Elaf Abdul-Wahab Hamdi ايلاف عبد الوهاب حمدي
Journal: Annals of the College of Medicine Mosul مجلة طب الموصل ISSN: 00271446 23096217 Year: 2012 Volume: 38 Issue: 2 Pages: 73-79
Publisher: Mosul University جامعة الموصل

Loading...
Loading...
Abstract

ABSTRACTObjectives: The aim of this study, first, to evaluate the frequency of immunohistochemical expression of p53 in different types of ovarian tumors, second, to correlate p53 expression with age of patients, histological type and grade of tumors, and third, to compare the results with those of others.Methods: This study was performed on 60 primary ovarian tumors. Samples were obtained in a prospective and retrospective fashion (cross sectional study). The samples were collected from Al-Jumhuri Teaching Hospital, Al-Salaam General Hospital, Al-Khansaa Teaching Hospital, and some private Laboratories, during a period of eight months, from June 2009 through January 2010. P53 expression was assessed immunohistochemically. Results: The patients' age ranged from 13 to 80 years, with a mean of 43.88 year. Most of them were in the fifth decade (28.3%). Data concerning the age were missing in five patients. P53 immunoreactivity was observed in 48.1% of the malignant tumors. It was positive in 13 of 20 cases of serous adenocarcinoma, in all of the 5 cases of mucinous adenocarcinoma, in 3 of 12 granulosa cell tumors, in 2 of 4 endometrioid carcinomas, in 1 of 3 clear cell carcinoma, in 1 of 2 dysgerminomas, and in the one case of Sertoli-Leydig cell tumor. It was negative in malignant thecoma, malignant teratoma and in all borderline and benign tumors. Statistically p53 expression was not significantly related to the age of the patients, grade, or to the histological type of the tumors. It was mainly found in malignant serous tumors (50%), in the poorly differentiated tumors (47.6%), and in the 6th decade of age (30.8%).Conclusions: P53 was expressed in 48.1% of malignant ovarian tumors, 80.8% were epithelial tumors, 15.4% were sex cord-stromal tumors, and 3.8% were germ cell tumors, and it was negative in benign and borderline tumors.

الخلاصةالأهداف: تم إجراء هذه الدراسة لتقييم حالة الظهور المناعي لبروتين p53 في أورام المبيض في مدينة الموصل والربط بينها وبين مختلف الصفات المرضية السريرية للورم ومقارنة هذه النتائج مع نتائج لدراسات أخرى.الحالات والطرق: إن هذه الدراسة مستقبلية ورجعية تم من خلالها جمع 60 حالة من أورام المبيض (49 حالة من سرطان المبيض، 5 حالات من أورام المبيض المتوسطة، بالإضافة إلى 6 حالات من أورام المبيض الحميدة). تم جمع هذه الحالات من مستشفى الجمهوري، السلام العام، الخنساء التعليمي، وبعض المختبرات الخاصة خلال فترة 8 أشهر امتدت من شهر حزيران 2009 إلى شهر كانون الثاني 2010. لقد تم التحري عن بروتين p53 بطريقة مناعية-نسيجية-كيميائية.النتائج والاستنتاجات: لقد تراوحت أعمار المرضى بين 13 و 80 سنة. وتم تقسيم الحالات السرطانية نسيجيا إلى 32 حالة لسرطان المبيض السطحي الظهاري و 3 حالات لسرطان الخلايا الجنسية (التناسلية) و14 حالة للسرطان المتعلق بالسدى والجنس و 5 حالات من أورام المبيض المتوسطة. بالإضافة إلى 6 حالات من أورام المبيض الحميدة. وقد ظهر بروتين p53 في 26 حالة (48.1%) من حالات سرطان المبيض و أورام المبيض المتوسطة. لم يكن هناك علاقة معنوية بين ظهور بروتين p53 وعمر المريضة أو مرتبة الورم أو النوع النسيجي المرضي للورم (P=0.2806، P=0.8710 ، P=0.0681 بالتعاقب). كما أظهرت النتائج عدم ظهور بروتين p53 في أورام المبيض المتوسطة والحميدة.

Listing 1 - 10 of 82 << page
of 9
>>
Sort by
Narrow your search

Resource type

article (82)


Language

English (76)

Arabic and English (6)


Year
From To Submit

2019 (3)

2018 (10)

2017 (14)

2016 (16)

2015 (9)

More...