research centers


Search results: Found 5

Listing 1 - 5 of 5
Sort by

Article
Immunological Effect of Polysaccharide Extracted from Pseudomonas aeruginosa against Leishmania donovani in mice

Author: Hasna H. Jumah
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 3 Pages: 265-278
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Visceral leishmaniasis is a serious disease with high pathoogenicity. It was noticed that Polysaccharide extracted from Pseudomonas aeruginosa had the ability to induce both cellular & humoral immunity response against bacteria, fungi & parasites.OBJECTIVE:The aim of this study was to know the immunological effects of polysaccharide extracted from Pseudomonas aeruginosa before & after the infection of BALB/c mice with Leishmania donovani in trial to provide a method for prevention of the disease in human.METHODS:174 male BALB/c mice were divided into control & experimental groups with each group consisting of six animals. The experimental groups injected intraperitoneally (i.p) with 0.2 ml phosphate buffer saline (PBS) containing 1×108 parasite. Cerdain groups were injected intraperitoneally by polysaccharide (PS) at doses of 100, 150, 250 μg on day 3, 6, and daily for 6 days before infection with Leishmania donovani. These animals were sacrified after 8 weeks. Other groups were injected intraperitoneally with polysaccharides on day 7 post infection at the doses of 100, 150, 250, μg, 6 animals were sacrified after 2, 4, 6, 8 weeks. Total & differential count of leukocytes, measurement of spleen & liver weight and index, measurement of cellular immune response, histopathological changes liver and spleen were carried out.RESULTS:This study showed an increase in the total count of leukocyte accompanied by an increase in lymphocyte with decrease in monocyte percent in mice treated with polysaccharide before and after infection with Leishmania donovani which were statistically significant. Reduction in liver & spleen weights & their index in all treated groups was noticed before & after infection. The cellular immune response represented by delayed hypersensitivity has been enhanced in the mice treated with polysaccharide before &after infection. In treated groups, histopathological studies of mice showed hepatocyte hypertrophy, infiltration of the inflammatory cells in liver before & after infection. In spleen showed an increase in lymphocytes & monocytes in red pulp, while the infected mice (non treated) showed a necrotic foci, loss of architecture of both liver & spleen & obvious granulomatous inflammation in liver.CONCLUSION:Polysaccharidde extracted from Pseudomonas aeruginosa induced immunological activity by activation of cell mediated responses, stimulated an increase in WBC count & lymphocytes with minor histoptholgical changes in liver and spleen.


Article
Immune Effect of Viable and Heat Killed Lactobacillus acidophilus in Mice Infected with Salmonella typhimurium

Author: Zahraa K. Zedan
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2012 Volume: 15 Issue: 4 Pages: 156-160
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

This study was designed to evaluate the immunological effect of viable and heat killed Lactobacillus acidophilus in mice infected with Salmonella typhimurium. The immunological parameters were total and absolute counts of leucocytes and phagocytic index of peritoneal cells. Twelve mice were divided into four groups. Each group consists of 3 mice. Group 1 was used as a negative control. Group 2 was infected with Salmonella typhimurium and use as positive control. Group 3 was fed with viable Lactobacillus acidophilus culture, and infected with culture of Salmonella typhimurium. Group4 was fed with heat killed Lactobacillus acidophilus culture, and infected with Salmonella typhimurium. Results indicated that treatment mice with viable Lactobacillus acidophilus were effective in enhancing the immune responses against Salmonella typhimurium as compared to negative (distilled water) and positive (Salmonella typhimurium) controls. While, mice fed with heat killed Lactobacillus acidophilus showed no enhancing immune effect on mice infected with Salmonella typhimurium

اجريت الدراسة لمعرفة التأثير المناعي لبكتريا acidophilus Lactobacillus المقتولة بالحرارة على الفئران المصابة ببكتريا السالمونيلاSalmonella typhimurium. درس التأثير المعزز المناعي الناتج من بكترياacidophilus Lb الحية والمقتولة بالحرارة من خلال دراسة التعداد الكلي والتفريقي لكريات الدم البيضاء وعملية البلعمة داخل الجسم الحي على 12 فأرة بيضاء والتي تم تقسيمها عشوائيا الى اربعة مجاميع 1 و2 و3 و4 وقد تضمنت كل مجموعة على ثلاثة فئران متساوية بالاعمار والاحجام. عدت المجموعة (1) التي تم تغذيتها على العليقة الاساس (مجموعة سيطرة سلبية)، اما المجموعة (2) ، ثم اصابتها بـ 0.1 مل من بكتريا S.typhimurium بعدد خلايا (2.5×10 7cfu/ml) ، بينما تم تغذية مجموعة (3) بـ 0.1 مل من بكترياacidophilus Lb الحية بعدد خلايا (1×10 9 cfu/ml )، وتم اصابتها بـ 0.1 مل من بكتريا S.typhimurium بعدد خلايا (2.5×10 7 cfu/ml) اما المجموعة (4) فقد تم تغذيتها بـ 0.1 مل من بكتريا acidophilus Lb المقتولة بالحرارة بعدد خلايا (1×10 9 cfu/ml)، ثم اصابتها بـ 0.1مل من بكتريا S.typhimurium بعدد خلايا (2.5×107 cfu/ml). اظهرت النتائج ما يأتي: التأثير المعزز المناعي في الفئران التي جرعت ببكترياacidophilus Lb الحية كان فعالا مقارنة مع مجموعة السيطرة بينما اظهرت الفئران المعاملة ببكترياacidophilus Lb المقتولة بالحرارة انخفاضا معنويا في التعداد الكلي والتفريقي لكريات الدم البيضاء وعملية البلعمة بالمقارنة مع مجاميع السيطرة.


Article
Micronucleus formation assay and phagocytic index in mice administered water leaf extracts of Camellia sinensis

Authors: Bushra H. Saleh بشرى صالح --- Rukaya M. Ebraheem رقية محمد ابراهيم --- Shahlaa M. Salih شهلاء صالح
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 3 Pages: 355-358
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Green tea Camellia sinensis is non fermented tea. The tea is an infusion of flavorful leaves that has been consumed for centuries as a beverage and is valued for its medicinal properties. Over the last few decades green tea has been subjected to many scientific and medical studies to determine the extent of its long-purported health benefits, with some evidence suggesting regular green tea drinkers may have lower chances of heart disease and developing certain types of cancer. Green tea has also been claimed useful for weight loss management.Methods: Three doses of the hot water extract were used (19.2, 38.4 and 57.6 mg/kg) to investigate micronucleus formation and phagocytic index. Mice were divided into two groups. Group one; control and group two; mice were administered with three doses of the green tea extract orally as a single dose (0.1 ml) per day for 7 days. Then the mice were sacrificed in day 8 for immunological and cytogenetic assessments.Results: Results revealed that the first and third doses of green tea hot water extract were significantly effective in enhancing the values of phagocytic index and reducing micronucleus formation.Conclusions: Hot water may be a good solvent to extract active component from Camellia sinensis especially catechin, polyphenols, polysaccharides, flavonoids and vitamins. The high dose of hot water extracts of Camellia sinensis showed an excellent enhancing effects on the function of immune system of mice, moreover, reduction in micronucleus formation was recorded at such dose.Key words: Green tea, Cytogenetic, Immunological effect


Article
Effect of IL-17Single Nucleotide PolymorphismsGene on the Risk of Developing Colorectal Cancer in Iraqi Patients

Author: Haidar J. Muhammed1, Nassir N. Mohsun2,Aroub A.R. Al-Kaisi2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 2 Pages: 51-58
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Interleukin -17 pathway plays an important role in development of inflammatory bowel diseases (IBD). Moreover it has been shown that this interleukin involved in anti-tumor or pro-tumor of colorectal cancer (CRC). This study determined (70) patients male to female (60% - % 40)inflicted with colorectal cancer,range aged (<16-80) years which were collected from Baghdad Teaching Hospitals/ Medical City.The patients detected for anti – IL17 by enzyme linked immune sorbent assay (ELISA)and compared with (10) individuals as a healthy control.The results showed significant differences (p<0.05). The gene encoding for the cytokine IL17 has genetically polymorphic, which has (30%) single nucleotide polymorphisms (SNPs) (12out of 40) patients by single strand conformation polymorphism technique (SSCP). In conclusion, polymorphic gene IL17 has been revealed to be associated with its susceptibility of colorectal cancer by showing there was a relationship between polymorphic gene IL17and the occurrence of colorectal cancer as well as the clinical features of the disease especially in agedpatients.


Article
Pathological and Immunological changes induced in male rats treated with therapeutic doses of sustanon
التغيرات المرضية والمناعية المستحدثة في ذكور الجرذان المعاملة بالجرعة العلاجية للسستانون

Authors: Kh. H. Aljeboori خليل حسن زناد الجبوري --- A. B. Majhool احمد بهبول مجهول
Journal: Al-Anbar Journal of Veterinary Sciences مجلة الانبار للعلوم البيطرية ISSN: 19996527 Year: 2017 Volume: 10 Issue: 1 Pages: 52-57
Publisher: University of Fallujah جامعة الفلوجة

Loading...
Loading...
Abstract

This study was designed by using twenty male rates (Rattus Rattus) and divided equally into two groups each one (10 animels). First group treated with mixing of Sustanon and sesam oil (5 mg/kg.b.wt) by injection i/m for 60 days, Second group treated with 1ml of sesam oil only i/m. Both groups vaccinated with BCG vaccine. At 30th, 60th day animals respectively were sacrificed and lungs, liver, spleen, kidney and testes were taken for histopathology. mild lesions were seen in these organs during these periods in the sustanon treated group only. Good Immune response (humoral and cell mediated immunity) were found in both groups of rats at the end of first month and second months. Conclusion, mild pathological lesions together with good immune response in the sustanon treated group and in control- BCG group

في دراسة صممت على 20 جرذاً من الذكور, قسمت إلى مجموعتين الأولى 10 جرذاً حقنت بالعضلة بمزيج الأندروجين النباتي (السستانون) مع زيت السمسم كمذيب بجرعة والمجموعة الثانية 10 جرذاً حقنت بالعضلة بزيت السمسم 1 مل (5mg/kg.b.wt). بجرعة 0.1 مل داخل الجلد BCG منعت المجموعتين باللقاح. بعد (30 و60) يوماً من الحقن قتلت الحيوانات واخذت الاعضاء الكبد, الرئتين, الطحال, الكليتين, والخصيتين. لغرض الدراسة المرضية حيث لحضت افات طفيفه في كل الاعضاء في المجموعة المحقونة بالسستانون في تلك الفترات واستجابة مناعية خلوية وخلطية كذلك. نستنتج من الدراسة وجود تغيرات مرضيه طفيفة في أعضاء الفئران المحقونة بالساستانون مع استجابة مناعية خلوية وخلطية

Listing 1 - 5 of 5
Sort by
Narrow your search

Resource type

article (5)


Language

English (4)

Arabic and English (1)


Year
From To Submit

2017 (1)

2016 (1)

2012 (1)

2010 (1)

2006 (1)