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Article
Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood

Authors: Hadeel H. Dawud --- Entesar H. Ali
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 2 Part (B) Engineering Pages: 152-155
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

The activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with various concentrations of nanoparticles rangedbetween (1- 6) μg/ml. The results indicates a decrease in enzymatic activity withincreasing of nanoparticles concentration. After that, the concentration of bloodurea was measured using urease obtained from a standard kit, urease purified fromProteus mirabilis and the urease- gamma iron nanoparticles solution. Thecomparison was then made among the results of urea concentrations by statisticalanalysis using T-test. The results showed that there is no significant differencebetween the results obtained from urease standard kit and urease purified fromthe bacteria, this is due to the efficiency of urease purified from the bacteria. On theother hand, the results showed that there is a significant difference (P≤ 0.01) inurea concentrations obtained from urease- gamma iron oxide nanoparticlessolution due to the inhibition of the enzyme which lost its activity by nanoparticles.


Article
Sensitivity of Streptococcus mutans to Selected Nanoparticles (in Vitro Study)

Authors: Farah Abdul-Razzak Al-Bazaz فرح عبدالرزاق البزاز --- Nada Jafer MH. Radhi --- Kadhim Abed Hubeatir
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2018 Volume: 30 Issue: 1 Pages: 69-75
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Nanoparticles are clusters of atoms in a size range from (1-100) nm. Nano dentistry creates amazing useful structures from individual atoms or molecules (nanoparticles), which provides a new alternative and a possibly superior strategy in prevention and treatment of dental caries through management of dental plaque biofilms. The aim of the study was to test the sensitivity of Streptococcus mutans to different concentrations of hydroxyapatite and iron oxide nanoparticles suspension solutions, in comparison to chlorhexidine, and de-ionized water, in vitro.Materials and methods: Agar well technique was applied to test the sensitivity of Streptococcus mutans to different concentrations of hydroxyapatite and iron oxide nanoparticles compared with chlorhexidine 0.2% as a control positive and de-ionized water as control negative. Zone of inhibitions which is clear zone of no growth of the bacteria were measured across the diameter of each well, no zone indicated a complete resistance of bacteria to the agents.Results: Values of mean of inhibition zone for all concentrations of hydroxyapatite nanoparticles were zero. While for iron oxide nanoparticles, they were zero until reaching the last three concentrations, in which there was a respective increase with a highly significant difference between groups (p>0.01). When making multiple comparisons of the inhibition zones of iron oxide nanoparticles between groups, findings showed that the inhibition zones of 17%, 20% and 22.5% of iron oxide nanoparticles were more than all other concentrations that had no inhibition zones with a significant difference (p>0.05). There was a highly significant difference between each concentration of hydroxyapatite and iron oxide nanoparticles with chlorhexidine and de-ionized water (p>0.01).Conclusion: Streptococcus mutans were not sensitive to hydroxyapatite nanoparticles, as there was a complete resistance for the agent. While for iron oxide nanoparticles, Streptococcus mutans were sensitive to 17.5%, 20% and 22.5% and sensitivity increased with the increase in concentration with a statistically highly significant difference and this indicates an antibacterial activity of this material


Article
Growth inhibition of mice mammary carcinoma cell line with green synthesized Magnetic Iron Oxide Nanoparticles

Authors: Amer T. Tawfeeq --- Ghassan M. Sulaiman --- Amal Naji
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2017 Volume: 10 Issue: 2 Pages: 234-238
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Mammary adenocarcinoma represents one of the most cancers that effect woman in Iraq and worldwide. Finding a definitecure for this type of cancer is a target of considerable research in many laboratories over the globe. In this study, a greensynthesized magnetic iron oxide nanoparticles (MNP) was used to specify their ability to inhibit the growth of mice mammarycarcinoma cell line (AMN3), it comes as a preliminary study of wider ongoing research. After successfully synthesizing magneticiron nanoparticles, it employed in different concentrations to expose AMN3 cells for 24 and 48 hours. The viability of theexposed cells was determined using standard MTT assay. The inhibition concentration 50 was determined afterward and usedto test the possibility of inducing apoptosis in the carcinoma cells using a mixture of fluorescent stains acridine orange andpropidium iodide. Results indicated that green synthesized MNP can inhibit the growth of mammary adenocarcinoma cell lineup to 79% and 89% after 24 and 48 hr of incubation with 1000μg/ml. The IC50 of MNP was capable of inducing apoptosis inthis cell line as shown by fluorescence microscope. The standard hemolysis assay used to test the reaction between the greensynthesized magnetic oxide nanoparticles and normal cells. The IC50 of AMN3 cells growth inhibition only induce 14% ofRBC hemolysis, while the percentage induced by negative control was 10%. In conclusion, green MNP have an effect uponcancer cells greater than normal cells. This material must tested against other types of cancer and normal cells to verify itsaction and study mechanisms of apoptosis induction in detail.


Article
Molecular and Nanotechnical Study for Antibiofilm Formation and CsuE Gene Expression Activities of Synthesized Iron Oxide Nanoparticles Against Multidrug-Resistant Acinetobacter baumannii Isolates

Author: Haider Turky AL-Mousawi1 , Mohammed I. AL-Taee2 , Maarib N. Rasheed2 , Qabas Nima AL-Hajjar3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 201-2015
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to investigate the influence of synthesis of Iron Oxide Nanoparticles (Fe3O4NPs) by co-precipitation method on biofilm formation and CsuE gene expression in multidrug resistance Acinetobacter baumannii (MDRA.b) which represents one of the important causing agents of nosocomial infection. A. baumannii isolates used in current study and isolates from different clinical sources (wounds, burns, urine, sputum, blood and throat) that able to produce strong biofilm. Synthesis and characterisation the physical-chemical nature of Fe3O4 NPs was carried out using UV-visible spectrophotometer, fourier transform infrared (FT-IR), X-ray diffraction (XRD), Atomic force microscope (AFM) and scanning electron microscope (SEM). Results show that they were very fine crystalline sizes reaching to 11±1 nm by XRD ,with a mostly spherical in shape and average of nanoparticles size between (40- 47) nm by SEM and AFM, respectively. Minimum inhibitory concentration (MIC) and sub-MIC test of Fe3O4 NPs at concentrations (15.75 to 2000) μg/ml against A.baumannii isolates had been determined using tube broth method measured by optical densities values at 630 nm. The results of the experiment of the best sub-MIC with concentration of Fe3O4 NPs 125μg/ml showed a significant difference at p˃0.05 the anti-biofilms inhibitory on polystyrene surface of microtiter plates for the bacteria isolate under study. Biofilm formation CsuE gene expression was investigated by using RT-qPCR technique with reference 16SrRNA gene before and after treatment with Fe3O4 NPs. The results showed a significant difference at p˃0.05 in Cycle threshold (Ct) values for CsuE gene expression of the isolates. The study concludes that the synthesized magnetic Fe3O4 NPs with 125µg/ml gave excellent antibiofilm activities and inhibitory efficiency against A.baumannii isolates and the potentiate to down regulate of gene expression fold for biofilm formation encoded by CsuE gene of A.baumannii leading as a result to have low biofilm production.

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