research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
The Correlations between JAK2V617F Mutational Status and Serum Levels of Folate and B12 in a Group of Patients with Chronic Myeloproliferative Neoplasms

Author: Haithem Ahmed Al-Rubaie
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2015 Volume: 14 Issue: 2 Pages: 274-280
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND: The Philadelphia-negative classical chronic myeloproliferative neoplasms are characterized by proliferation of one or more cell lines in the bone marrow and increased number of mature and immature cells in the peripheral blood. These myeloproliferative neoplasms include; polycythemia vera, essential thrombocythemia and idiopathic myelofibrosis, in which JAK2V617F is by far the most prevalent mutation. Because of the rapid turnover of cells in these neoplasms, derangement in the serum levels of vitamin B12 and folate is expected. OBJECTIVE: To assess the possible correlation of serum B12 and folate levels with JAK2V617F mutation in patients with polycythemia vera, essential thrombocythemia and idiopathic myelofibrosis.PATIENTS AND METHODS:This case-control study was conducted from December 2012 to December 2013, and enrolled 54 patients, diagnosed as polycythemia vera (36), essential thrombocythemia (6) and idiopathic myelofibrosis (12), attending Medical City, Baghdad Teaching Hospital. Twenty healthy volunteers were included as a control group. JAK2V617F mutation status had been reviewed at time of sampling. The following investigations were done: automated complete blood counts; serum B12 and folate were measured using the electrochemiluminescence immunoassay. RESULTS:The mean levels of serum B12 and folate were much higher in myeloproliferative neoplasm patients than control group (p-value of 0.028 and 0.004 respectively).Serum B12 level showed significant difference between polycythemia vera patients with positive JAK2V617F and negative mutation (p= 0.04), while no significant difference was found for serum folate level (p= 0.630). Insignificant differences were also found for idiopathic myelofibrosis patients (B12, p= 0.140; and serum folate, p= 0.098), and essential thrombocythemia patients (B12, p= 0.133; and serum folate, p= 0.800).Correlations of serum folate and B12 with hematocrit, white blood cell count, absolute neutrophil count, platelet count, mean cell volume, and mean cell hemoglobin in myeloproliferative neoplasm patients: significant correlations were only found in PV patients between B12 and total white blood cell count, absolute neutrophil count and platelet count (p= 0.009, 0.012 and 0.002 respectively).CONCLUSION:There is derangement in the levels of serum B12 in patients with polycythemia vera in relation to JAK2V617F mutation, thus we may propose that this mutation may have a possible impact on polycythemia vera patients, in particular, reflected by higher levels of serum B12 through the associated increase in absolute neutrophil count.


Article
Estimate of JAK 2 V617F Mutation and Activity of Catalase Enzyme in Myeloproliferative Neoplasm

Authors: Heba Rushdi Abdullah --- Perry Habib Saifullah --- Ziad Ahmed Shebib --- Ahmed Rushdi Abdullah
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2016 Volume: 19 Issue: 3 Pages: 1-8
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background: Myeloproliferative neoplasms (MPNs) are a range of clonal hematological diseases with overlapping features, a specific mutation in the JAK2 gene, which encodes a tyrosine Kinase has been shown to be associated with the myeloproliferative phenotypes (PV, ET and PMF). Aim: Estimate the proportion of JAK2 V617F mutant gene and estimate the significance of antioxidant enzyme (Catalase) is mainly associated with increased oxidative stress this is resulting increase of the free oxygen radicals and relate it with suspected myeloproliferative neoplasms (MPNs) in Iraqi patients. Materials and Methods: Total of (51) patients with suspected myeloproliferative neoplasms (MPNs) and 20 healthy individuals were analyzed for the JAK2 V617F mutation. After DNA extraction, detection of the mutation was done using (ARMS) PCR amplification, activity of Catalase was measured spectrophotometrically. Results: Of 51 patients, the JAK2 V617F mutation (V617F) was detected in 33 out of 42, with PV (81%), and four of patients with ET and PMF (40% and 50%, respectively). The prevalence of this mutation is more associated with male than female about (62%). Catalase activity was found highly significant (P< 0.01) among suspected MPN patients when compared with control group. Conclusion: JAK2 V617F mutation screening can be incorporated in the initial estimate of patients suspected of having MPNs. Catalase enzyme used a biomarker of enzymatic alteration in MPN Iraqi patients.

اورام التكاثر النقيي (MPNs) هي سرطانات تعرف باضطربات نسليه لانتاج الخلايا الدمويه مع زيادة إنتاج نوع واحد او اكثر من خلايا الدم الناضجة من انساب الدم النخاعي, و التي يمكن تصنيفها إلى: زيادة انتاج الكريات الحمراء الاولي (PV)، زيادة الاقراص الدمويه الأساسية (ET)، وتليف نخاع العظم الاولي (PMF).تهدف هذه الدراسة الى دعم تشخيص المرض بطريقة دقيقة وذلك بتقدير نسبة الطفرة الجينيه (JAK2 (V617F و قياس انزيم مضاد الاكسدة (الكاتليز) المرتبط بصورة اساسيه مع زيادة الاكسدة الاجهادية والناتجة من زيادة جذور الاوكسجين الحرة للمرضى المصابين بورم التكاثر النقيي (MPNs) وعند مقارنتهم مع الاشخاص الاصحاء, حيث تم تقدير نسبة الطفرة الجينية (JAK2 (V617F وقيمة انزيم الكتاليز عند (51) مريضا بورم التكاثر النقيي و(20) من الاشخاص الاصحاء.أظهرت نتائج الدراسة عند المرضى المصابين حديثا ان الطفرة الجينية( JAK2 (V617F هي إيجابية في 90٪ من مرضى زيادة كريات الدم الحمراء الاولي و 50٪ من مرضى تليف نخاع العظم الاولي و 40٪ من مرضى زيادة الاقراص الدموية الاساسيه. وظهر لدينا بوجود فرق معنوي عالي لمعدل قيم انزيم الكتاليز (0.01P<) عند المرضى بالمقارنة مع الاشخاص الاصحاء. استنتجت الدراسة الحالية ان طفرة الجين( JAK2 (V617F وجدت في 82% عند مرضى ورم التكاثر النقيي (MPN) وان المشتقات التفاعلية للاوكسجين له دور اساسي في التسرطن وتطور اورام التكاثر النقيي (MPNs).

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2016 (1)

2015 (1)