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Article
Serum MicroRNA-21 High Level Expression in Breast Cancer in Relation to Advance Stage

Author: Shoroq Mohammed AL-Temimi *, Adel Mosa AL-Rekabi **
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2017 Volume: 16 Issue: 1 Pages: 9-17
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:MiR-21 plays an important role in tumor formation and development , and it has been shown to be a key regulator of oncogenic processes and it may be a useful biomarker for breast cancer .OBJECTIVE: Estimation of miR-21 gene expression levels in both fresh tissues and serum of same breast cancer patients before and after operation by using stem-loop followed by Taq-Man Real Time PCR (RT-PCR) technique and correlate the miR-21 gene expression with stages of breast cancer and lymph node involvement. MATERIAL AND METHODS:Stem-loop RT-PCR was performed to identify the level of miR-21 gene expression in both fresh tissues and serum of same breast cancer patients before and after operation . The expression levels of miR-21 relative to mRNA of GAPDH were determined by using the livak method. RESULTS:Mean fold change of miR-21 was significantly higher in breast cancer than that of paracancerous tissues . Before operation ,the mean fold change of serum miR-21gene expression significantly statistical difference from healthy control ,the mean fold change of miR-21 in advance stage( III,IV) was significantly higher than that early stage (I,II) and mean fold change of miR‑21 in positive lymph node was higher than that of negative lymph node . After operation the mean fold change of serum miR-21gene expression was significantly higher than healthy control .A statistically significant difference was noted between advance stage with lymph node involvement and early stage with negative lymph node involvement .In contrast , the mean fold change of serum miR-21 was significantly lower from that before operation .CONCLUSION:Circulating miR-21 before and after operation can serve as a good biomarker for breast cancer detection and progression .


Article
Immunohistochemical Localization of Epstein Barr Virus- Latent Membrane Protein 1 in Breast Cancer Tissues

Author: Saad Hasan Mohammed Ali *,Shakir H. Mohammed Al-Alwany**, Ghanim I.A. Al-Wadi *
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 234-240
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACK GROUND:Epstein- Barr virus (EBV) is well known to infect the vast majority of the world's adult population and has recently been reported in many literatures for its association with a broad spectrum of benign and malignant tumors, including breast cancer. OBJECTIVE: To analyze the relations and impact of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) expression on a group of tissues with primary invasive breast cancers.PATIENTS AND METHODS: Forty- nine (49) formalin-fixed, paraffin- embedded breast tissues were obtained in this study; (34) biopsies from breast cancers (BC) and (15) from apparently normal breast autopsies control group. Detection of expression of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) was done by HRP/DAB immunohistochemistry detection kit (an immunoenzymatic antigen detection system for immunohistochemistry techniques) using specific primary antibodies for EBV-LMP 1.RESULTS: Detection of EBV-LMP 1 - immunohistochemical (IHC) reactions in tissues with BC was observed in 11 out of 34 (32.4%), while in healthy breast tissues in the control group was detected in 13.3% (2 out of 15). The difference between the percentages of EBV-LMP 1 detection in BC tissues & control group was statistically not significant (P >0.05).While no significant differences were observed between EBV-LMP 1 detection in BC tissues & the age of patients as well as grade of invasive breast cancers (P value > 0.05).CONCLUSION: Our results indicate that EBV could have a role in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis. .

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