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Article
Immunohistochemical expression of HOXA1, and Ki-67 proteins of oral squamous cell carcinoma

Authors: Ibtehal Qhtan Al-Etbi ابتهال قحطان --- Riyadh Othman Al-Kaisi رياض عثمان القيسي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 2 Pages: 74-78
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral squamous cell carcinoma (OSCC) is the most prevalent malignant neoplasm of the oral cavityand constitutes a major health problem in developing. In the last 30 years, the 5-year survival rate of patients withoral SCC has not improved despite advance in diagnostic techniques. To improve early diagnosis for this deadlydisease, new biological markers are needed. HOX genes encode homeodomain-containing transcription factorsinvolved in the regulation of cellular proliferation and differentiation during embryogenesis. HOX gene expression hasbeen described in several adult tissues, where they performed important roles in maintaining homeostasis. Fewstudies have suggested that HOXA1 plays a role in tumorigenesis. Besides being overexpressed in several tumors,HOXA1 influences numerous cellular processes including proliferation, apoptosis and epithelialmesenchymaltransition (EMT), and HOXA1 overexpression is sufficient for malignant transformation ofnontumorigenic epithelial cells.Ki-67 is a specific marker of proliferation and the expression of which is strictly associated with cell proliferation and iswidely used in pathology as a proliferation marker to measure the growth fraction of cells in human tumors.The aimsof this study were to evaluate the immunohistochemical expression of HOXA1 & Ki-67 in OSCC & to correlate theexpression of the studied markers with the clinicopathological findings and with each otherMaterials and Methods: Thirty formalin-fixed, paraffin- embedded tissue blocks of oral squamous cell carcinoma wereincluded in this study. H&E stain was done for each block for reassessment of histological examination. Animmunohistochimical stain was performed using anti HOXA1 and anti Ki-67 poly clonal antibodies.Results:The expression of HOXA1 and Ki-67were positive in all oral squamous cell carcinoma cases & in all layers(100%), while the expression was restricted to the basal and supra basal layer in normal oral mucosa. Statisticallynon-significant correlation observed between each marker with clinico-pathological parameters. While a statisticallysignificant association was found between the expressions of two markers, (p-value= 0.027).Conclusion: The statistically significant association observed between expressions of HOXA1 with the specific markerof proliferation Ki-67. This suggested important role in oral SCC development and progression.

الخلفیة:سرطان الخلایا الحرشفیة ھو السرطان السائد في التجویف الفمي ویمثل المشكلة الرئیسیة المؤدیة للوفاة في بلدان العالم الثالث. وبالرغم من تطور التقنیات الطبیة التشخیصیة إلا إنمستوى سنوات البقاء الخمسة المعتمدة في علم الأورام لم یتطور بشكل مفید في السنوات الثلاثین الاخیرة. إن الكشف المبكر لسرطان الخلایا الحرشفیة الفموي مھم جدا للحد من خطورتھالمحفزة للنمو والتمایز اثناء النمو Homeobox التابعة لجینات HOX وھو احد افراد عائلة جین HOXA ولذلك تم التركیز على إیجاد واسمات بیولوجیة جدیدة ومنھا جین 1الجنیني والتكوین العضوي وقد یظھر في الأنسجة البالغة عند الحاجة ایضا. وقد اثبتت دراسات حدیثة دوره المسرطنالفعال في العدید من الامراض السرطانیة حیث یظھر بشكل غیرھو مؤشر التكاثر الرئیسي في النواة وھو المساعد في كشف وجود اي انقسامات في الأنسجة وبالتالي فلھ فائدة عظیمة في التنبؤ والكشف المبكر لسرطان Ki- متوازن في الأنسجة . 67وكذلك ربط ظھور كل منھما مع . Ki- في السرطان الحرشفي للفم وربط ظھور الجین بمؤشر التكاثر 67 HOXA الفم. تھدف الدراسة الحالیة الى التحري والتحقق من ظھور جین 1المعطیات السریریة المرضیة لسرطان الفم الحرشفي.المواد والطرق: تضمنت ھذه الدراسة ثلاثین عینة استرجاعیة لأشخاص مصابین بسرطان الفم الحرشفي والتي استخرجت من المقاطع النسیجیة المثبتة بالفورمالین والمطمورة بشمعالبارافین وجرى صبغ كل عینة بصبغتي الھیماتوكسلین والایوسین لإعادة تقییمھا لغرض الفحص النسیجي المرضي. بعد لك اجریت الصبغات الكیمیائیة النسیجیة المناعیة باستخدامعلى شرائح نسیجیة دقیقة من العینات. Ki- ومضاد 67 HOXA مضاد 1النتائج: أظھرت الدراسة أن اكثر حالات ھذا السرطان تقع في الاعمار التي تفوق الخمسین عاماً وأن معظم تلك الحالات تركزت في الذكور وبنسبة ( 70 %) .اما نسبة إصابة الذكور(% الى الاناث فقد اظھرت ھذه الدراسة إلى انھا تساوي:( 1:2 ). كذلك اظھرت الدراسة ان معظم الحالات كانت في اللسان ( 36,7 %) ومعظمھا ظھرت سریریا بشكل اورا م ( 73,3.اما الفحوصات النسیجیة المرضیة لھذه الدراسة فقد أظھرت ان ( 43,3 %) من الحالات السرطانیة ھي من النوع المتوسط التمایز و( 40,0 %) من النوع الواضح التمایز لسرطان الفمكان ایجابیاً في الطبقة السفلى فقط من النسیج المخاطي الفموي الطبیعي، بینما كان ایجابیاً في كل Ki- ومؤشر التكاثر 67 HOXA الحرشفي . اظھرت ھذه الدراسة ایضاً ان تعبیر 1HOXA مما یدل على الدور المھم لجین 1 Ki- ومؤشر التكاثر 67 HOXA طبقات النسیج الحرشفي لسرطان الفم. كذلك اظھرت ھذه الدراسة وجود علاقة واضحة بین ظھور جین 1كعامل مسرطن وذلك بواسطة تحفیز انقسام الخلایا وبالتالي النمو السرطاني الفموي واخیرا بینت ھذه الدراسة عدم وجود ایة علاقة بین العاملین السابقین والمتغیرات السریریة المرضیةالاخرى المصاحبة لسرطان الفم الحرشفي.في انقسام الخلایا والنمو السرطاني لذلك تقترح ھذه الدراسة اجراء دراسات HOXA دلالة على دور جین 1 Ki- مع مؤشر التكاثر 67 HOXA الاستنتاجات: تلازم وجود جین 1او مع مؤشر تكاثر اخر. Ki- مع مؤشر التكاثر 67 HOX او عضو اخر لجین HOXA جدیدة بعینات اكثر عددا لمعرفھ الدور الحقیقي لجین

Keywords

OSCC --- HOXA1 --- Ki-67


Article
Effect of Aspartame on the Liver of Male Albino Rats: A Histopathological and Immunohistochemical Study

Authors: Nali Abd Al-Qadir Maaruf --- Zana Mostafa Mahmood --- Tenya Tariq Abdulhameed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 558 -566
Publisher: Babylon University جامعة بابل

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Abstract

Aspartame is a common artificial sweetener used today. It has a brand name NutraSweet® and Equal®, is produced by union of amino acids, aspartic acid and phenylalanine.It is widely used in many beverages and food while after metabolites in gastrointestinal tract it causes harmful effect to several organs specially the liver, as the liver considered as the largest organ in the abdomen and its function includes working with the pancreas and intestines to process, absorb food, filtering blood from the digestive tract and detoxification of drugs and alcohol. Ki-67 is a nuclear protein antigen which is related to and may be important for proliferation of cells. The aim ofthe study was to detectthe effect of aspartame on the hepatic tissue of male adult rats by noticing any histopathological change and also to observe the localization of Ki-67 protein after giving aspartame orally. Fiftymale albino ratsused in this study, they were divided into two groups: Group A 10 rats, were received distilled water and regarded as control. Group B 40 rats, administrated 40mg/Kg aspartame powder after dissolving it in 2ml distilled water orally daily for six weeks and regarded as treated group. After completion of the experimentthe animals were sacrificed. Piecesof liver were fixed in 10% neutral buffered formalin and embedded in paraffin wax. Sections, which were 4-5 micrometers thick, made then stained with haematoxylin and eosin for histo-pathological examination. Immunohistochemical examination was done by using Ki-67 antibody and the standard streptavidin-biotin immunoperoxidase method. In the treated group, the hepatic plates losttheir normal radial arrangement around central veins. The hepatocytes lost their polygonal shape and showed many cytoplasmic vacuoles of variable size; while the localization of Ki-67 was significantly increased in treated group rats.In conclusion receiving aspartame at a dose 40 mg/kg causes histopathological alterations of the hepatic tissues and leads to increase in the proliferation rate.

Keywords

Liver --- aspartame --- Ki-67.


Article
Expression of Ki 67 and P53 immunohistochemical markers in central nervous system astrocytoma
التعبير المناعي النسيجي الكيميائي للمعلمات(Ki 67 and P53) في اورام الخلايا النجمية للجهاز العصبي المركزي

Authors: Khitam R. Al-Khafaji ختام رزاق الخفاجي --- Mahmood Sh. Mahmood محمود شكر
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2014 Volume: 56 Issue: 4 Pages: 376-379
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Astrocytic tumors are the most common primary tumors of the central nervous system. Several grading systems are used to grade astrocytomas. The most widely used system is the World Health Organization (WHO) classification (1979, 1993, 2000, and 2007) that grades astrocytomas (I-IV) based on cytological atypia, mitotic activity, vascular proliferation, and necrosis: pilocytic astrocytoma (grade I), diffuse astrocytoma (grade II), anaplastic astrocytoma (grade III), and glioblastoma (grade IV). Objectives: The aim of this study is to evaluate p53 over expression , Ki-67 expression in astrocytomas and Correlate these two markers with histologic grade of astrocytomas.Methods: Formalin fixed, paraffin-embedded blocks from 40 patients with brain astrocytoma included in this retrospective study. LSAB (Labeled Strept-Avidin, Biotin) method was employed for immunohistochemical detection of Ki – 67 and P53.Results: P53 was detected in (25%) of the cases and was significantly positively correlated with grade IV. Ki-67 labeling index was (>5%) in (50%) of the cases. Both biomarkers were positively correlated with each other, and the grade of astrocytoma; however, Ki67 is a better marker for differentiating (diagnostic marker) between the grades of astrocytoma than p53. Conclusion: P53 overexpression and ki-67 expression plays an important role in pathogenesis of astrocytoma evolution, as they positively associated with higher tumor grade. Key words: Ki – 67, P53, Astrocytoma.

المقدمة: تعد أورام الخلايا النجمية أكثر أورام الجهازالعصبي المركزي شيوعا", الذي يكون فيه نوع الخلية السائد مشتق من الخلية النجمية. إن كل من (Ki-67 و P53) بروتينات خلوية لها دورا" في القابلية الامراضية وتطور درجة خبث أورام الخلايا النجمية. يعد تصنيف منظمة الصحة العالمية للسنوات (١٩٧٩ ١٩٩٣٬ ٢٠٠٠٬و٢٠٠٧)هو التصنيف الاوسع أستعمالا"لاورام الخلايا النجمية ويقسم هذه الاورام الى اربعة درجات.أهداف الدراسة : تقييم التعبير المناعي النسيجي للمعلمات (P53 و Ki-67) في أورام الخلايا النجمية ٬ وعلاقة هذه المعلمات مع درجة الورم النجمي وعلاقتهما فيما بينهما.المواد وطرق العمل : هذه الدراسة تمت بأثر رجعي, جمعت أربعون عينة نسيجية لمرضى مصابين بأورام الخلايا النجمية٬ صبغت العينات بطريقة (Dako- LSAB) التصبيغ المناعي النسيجي الكيميائي للمعلمات (Ki-67 و(P53٬ و اعتبرت قيمة P اقل من (% ٠.05) كقيمة ذات مغزى او دلالة احصائيا. النتائج:نسبة التعبير المناعي للمعلم (P53) كانت (٢٥ %) وأثبتت الدراسة أن هناك فرق ذا مغزى بين التعبيرالزائد ل (P53) والدرجة الرابعة للورم. نسبة التعبير المناعي للمعلم (Ki-67) لأكثر من ٥ % كانت (٥٠ %). هناك علاقة أيجابية بين التعبيرين المناعيين (Ki-67 و(P53 فيما بينهما ،علما" بأن التعبير المناعي Ki-67 هو الافضل للتفريق (تشخيص مناعي) بين درجات أورام الخلايا النجمية من التعبير المناعي P53 .الاستنتاج:من النتائج أعلاه يمكن لنا أن نستنتج بأن (Ki-67 وp53) يلعبان دور مهم في تولد ونشأة أورام الخلايا النجمية ويسند الدليل عن أرتباطهما مع زيادة درجة الورم وقابليته العدوانية الحيوية. مفتاح الكلمات: الاورام النجمية Ki-67 و(P53


Article
Ki 67 Expression in Various Diseases of Gallbladder

Authors: Salman Shafeeq Salman --- Alaa Hani Raziq --- Kareem Mohammed Ahmed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 3 Pages: 712 -721
Publisher: Babylon University جامعة بابل

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Abstract

Cell proliferation is an important prerequisite for the development of a neoplasm. Ki-67 is one of the most important cell proliferation markers. Its expression is correlated with the aggression of various histopathologic changes in the epithelium of gallbladder. This study aims to assess the expression of Ki-67 in various pathological processes that affects the epithelium of gallbladder.Gallbladders from 146 patients were studied histopathologically. Then sections from the paraffin embedded tissues were evaluated for the expression of Ki-67 by using immunohistochemical method. The results of Ki-67 labeling index were estimated for each pathologic category and were analyzed for their significance using Mann-Whitney test.The mean value of Ki-67 LI in the epithelium of normal gallbladder was (0.7%), in acute cholecystitis (1.14%), epithelial hyperplasia (8.38%), metaplasia (21.18%), dysplasia (38%) and (70.6%) in the carcinoma group. There was no statistically significant difference with regard to ki-67 between the epithelium of normal GB and those with acute inflammation (p= 0.904) and the difference was highly significant when the normal gallbladder epithelium was compared with carcinoma group. There was statistically highly significant difference when the comparison was performed between normal epithelium and metaplasia group (p< 0.001) and the group of dysplasia (p= 0.001).From the present study a conclusion can be made is that Ki-67 expression increases in chronic inflammation and further increment occurs in premalignant and malignant lesions of the gall bladder epithelium and can be used as a marker of aggression of histopatholgic lesions.


Article
Proliferative potential in benign mixed salivary gland tumors, using Ki67 marker in relation to different clinicopathological parameters
إمكانات تكاثرية في أورام الغدة اللعابية المختلطة الحميدة ، باستخدام علامة Ki67 بالنسبة إلى المعلمات الإكلينيكية المختلفة

Authors: Henan A. Abdulqadir --- Chenar M. Mousa --- Layla M. Muhammad
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 1 Pages: 1241-1248
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Background and objective: Pleomorphic adenoma is the most common benign tumor of the salivary gland. It shows a remarkable degree of morphological diversity. Immunohistochemical Ki67 expression is used to predict the proliferative potential of the growth. The aim of this study was to evaluate the expression of the proliferation marker Ki-67 in benign mixed salivary gland tumors. Methods: Fifty five paraffin embedded tissue blocks were included in the study. After histopathological reassessment of hematoxylin and eosin stained section for each block, an immunohistochemical staining was performed using anti-Ki67 protein. Results: From total number of 55 cases, 58.1% of patients were in group aged ≤ 40 years. The male to female ratio was 1.037:1. The parotid gland was the most affected (56.37%) gland. The mean duration of lesions before treatment was 17.96 months and the mean size of tumors was 3.76 cm. Histologically, cell-rich subtype constituted 54.54%, followed by stroma-rich (34.55%), and only 10.91% of cases were classic. Ki67 expression was positive in 18.18% predominantly in epithelial variant with a weak score in nine cases (16.36%). Six of positive cases were in parotid gland and two cases in the submandibular gland. Only one case was in minor salivary gland. Moderate Ki67 expression score was seen in one epithelial variant case (14.28 %) associated with parotid gland. Conclusion: Immunohistochemical expression of Ki-67 in pleomorphic adenoma was characterized by low proliferative rate and predominantly seen in epithelial tumor variants.


Article
Evaluation of epidermal growth factor receptor (EGFR), proliferation (Ki-67) and apoptosis (P53) in salivary mucoepidermoid carcinoma in relation to tumor grade

Authors: Saad A. Al-Ani سعد العاني --- Bashar Hamid Abdullah بشار حميد عبد الله
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 43-47
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. Theydisplay a variety of biological behaviours, and several systems have, therefore, been proposed to gradethis neoplasm. Today, the most popular grading systems are Auclair et al, (1992) and Brandwein et al,(2001)grading systems. Assessment of cellular proliferation, apoptosis and growth molecules are important factorsin tumor kinetic which may reflect tumor biological behaviour.Materials and methods: Immunohistochemical analyses of seventeen cases of -fixed paraffin-embeddedtissue blocks of MEC of salivary gland origin using (Ki-67, P53 and EGFR) monoclonal antibodies.Results: The samples comprised of ten males and seven females to give male to female ratio (1.4:1). Themean age was (47.06±8.5) years. The submandibular salivary gland was the most predominant affected site(5 cases). 100% of cases were EGFR immunopositive. Only 47% of MEC cases showed Ki-67 immunopositivity,while P53 immunopositivity were shown in 94% of MEC cases. There was no statistically significantcorrelation regarding P53 or EGFR markers in relation with grading systems. There was a statisticallysignificant correlation between the expression of Ki-67 marker and Auclair grading system. There were nosignificant statistical correlation among markers except between Ki-67 expression and P53.Conclusions: Assessment of tumor biology in term of apoptosis (p53), proliferation (Ki-67) and EGFR are notreflected on tumor grade


Article
Expression of Ki67 and p53 as proliferation and apoptosis markers in adenoid cystic carcinoma

Author: Lehadh M. Al-Azzawi لحاظ العزواي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 76-79
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Adenoid cystic carcinoma (ACC) constitutes about 4% of salivary epithelial tumors and is the secondcommon malignant epithelial salivary gland tumor involving both the major and minor salivary glands. Aims of thestudy is to evaluate immunohistochemical expression of Ki67 and p53 proteins in ACC.Materials and Methods: immunohistochemical analyses of fifteen cases of formalin – fixed paraffin – embeddedtissues blocks of ACC of salivary glands using ki67 and p53 antibodies.Results: ki67 was expressed in 6 of 15 ACC (40%) while p53 aberration was demonstrated in 11 of tumor (73.3%). Therewas a statistically significant difference between the expression of ki67 and p53 proteins in ACC cases (p value =0.041). Pearson’s correlation test demonstrated a significant positive correlation between the numbers ofpercentage of ki67 and p53 positive cells in ACC cases (r = 0.042).Conclusions: This study suggests that ki67 positive actively proliferating cells and p53 aberrations may play a role inACC development and progression


Article
The Application of ImmunohistoChemical Markers for Diagnosis, Classification and Grading of Neuroendocrine Tumors
تطبيق المعلًمات المناعية الكيميائية النسيجية لتشخيص وتصنيف وتحديد درجةأورام الغدد الصم العصبية

Authors: Kaswer Musa Al.Turaihi --- Roaahameed Al-Waidh
Journal: kufa Journal for Nursing sciences مجلة الكوفة لعلوم التمريض ISSN: 22234055 Year: 2014 Volume: 4 Issue: 3 Pages: 63-70
Publisher: University of Kufa جامعة الكوفة

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Abstract

objectives: To study the sensitivity of Chromogranin A in the diagnosis of neuroendocrine tumors from various locations in the body and its association with clinicopathological parameters like site and grade of tumors . methodology: This study is conducted in the Department of Pathology, Faculty of Medicine, University of Kufa from January - October 2013.A total of 50 patients with neuroendocrine tumors from different locations. their ages ranging between 22-80 years with a mean age of 48.3 years. Two step Envision TM flex method was employed forimmunohistochemical detection of ki67 antigen (MIB -1) and Chromogranin A .Results: Main site for patients with neuroendocrine tumors included in this study is gastroenteropancreatic system and there is female predominance , from 50 patients 44 (88%) cases was positive for Chromoranin A expression , there was no significant differences in expression of the Chromogranin A with location and grade of tumors (P value >0.05)Conclusion: Chromogranin A is highly sensitive marker in diagnosis of neuroendocrine tumors irrespective to tumors location and tumor grade .Recommendation: We advise to use Chromogranin A immunohistochemical marker as a main marker in diagnosis of neuroendocrie tumors in addition to that this marker can be used as target for new modality in tumor therapy .

الهدف: لدراسة حساسية ال( Chromogranin A) في تشخيصاورام الغدد الصم العصبية من مواقع مختلفة في الجسم وارتباطه مع المعايير المرضية والسريرية مثل موقع الورم والصف.المنهجية : طبقت هذه الدراسة على خمسين مريض مصاب بأورام الغدد الصم العصبية في قسم علم الأمراض، كلية الطب , جامعة الكوفة من كانون الثاني 2013 الى تشرين الأول 2013.. أعمارهم تتراوح ما بين 22-80 سنة وكان المعدل العمري 48.3. استخدمت طريقةTwo step Envision TM flexلتحديد التعبير المناعي لمستضد ki67 (MIB-1)و Chromogranin A. النتائج :كان الموقع الرئيسي للمرضى الذين يعانون من أورام الغدد الصم العصبية المنخرطين في هذه الدراسة هو الجهاز الهضمي المعوي والبنكرياسوكانت هناك هيمنة الإناث، كان التعبير المناعي لل Chromogranin Aهو 44 (88%) ولاتوجد علاقة ذات مغزى بين التعبير المناعي لل AChromograninمع الموقع والصف للأورام.الاستنتاجات :Chromogranin A معلم حساس للغاية في تشخيص أورام الغدد الصم العصبية بصرف النظر عن الموقع والصف.التوصيات : ننصحباستخدام المعلم المناعي AChromograninكمعلم رئيسي في تشخيص الأورام الغدد الصم العصبيةبالإضافة الى إمكانية استخدامه كهدف لنمط جديد في علاج الأورام.


Article
The Expression of P53 and ki67 in Colonic Polyps
ظهور المعلمات السرطانية p53 و Ki-67 في بولب القولون

Author: Liwaa H. Mahdi
Journal: kufa Journal for Nursing sciences مجلة الكوفة لعلوم التمريض ISSN: 22234055 Year: 2015 Volume: 5 Issue: 2 Pages: 173-180
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background: Colonic polyps are slow-growing overgrowth that projects above the surrounding mucosas of the colon that carry a small risk (< 1%) of becoming malignant. However, because colonic polyps are highly prevalent in the general population (especially with increasing age), they confer an important predisposition to colon cancer and are therefore removed when detected. The distribution of Ki67 and p53 as markers of cell proliferation is very studied in Colorectal Carcinoma. In Iraq, IHC studies to detect p53 & ki-67 expression in GIT malignancy has been conducted.Aim of the study: To detect the expression of p53 & ki-67 in colonic polyps patient by immunohistochemistry .To assess the possible correlation between p53 &ki-67 in colonic polyps patient.To detect the possibility of conversion of pre-malignant colonic polyps to malignant form.Methodology: Fifty cases(27 male and 23 female ) were selected randomly with different types of colonic polyps were included in this study and using discreptive study. The age of patients were ranging from 4 up to 80 years, with mean age of [ 46.3 ] years. Cases were collected from the laboratory of histopathology in Al-sadder Medical city in Al-Najaf and from some private laboratories in the same governorate(Al-Najaf) .the data analysed by qi sequare.Result: There is a significant correlation between P53 & age ,p53 & type of polyp also there is a significant correlation between Ki-67& age, Ki-67 & type of colonic polyp .There is a correlation between ki67 & p53.Conclusion :The study represent a trial to understand the probable conversion of polyps to colorectal carcinoma and the role of P53 & KI-67 in genesis of colorectal carcinoma. Recommendation: Screen and follow up to all patient with colonic polyps specially to patient with multiple polyps. All type of polyps should be removed ,because polypectomy currently constitutes the best strategy for preventing colorectal cancer

خلفية البحث: بولب القولون من أكثر الأمراض انتشارا في العراق ويعد سببا لحدوث سرطان القولون .ان كل من (( ki67&p53 بروتينات خلوية تلعب دورا في حدوث السرطان .KI-67:بروتين خلوي يظهر في نواة الخلايا المتكاثرة في الجسم في جميع الأطوار التكاثرية للخلية بدرجات متفاوتة ويفقد في الطور الصفري.P53:هو مورث يقع على الكر وموسوم السابع عشر يسيطر على انقسام الخلية ويعمل كمورث محبط للورم.هدف الدراسة:تقييم التعبير المناعي لعاملي P53,KI-67 في بولب القولون وإيجاد إمكانية تحول بولب القولون إلى سرطان القولون.المنهجية : طبقت الدراسة على خمسون مريض لديهم بولب القولون للفترة من 2011ولغاية 2014,تم جمع العينات من مستشفى الصدر التعليمي وبعض المختبرات الخاصة في النجف وكانت اعمار المرضى تتراوح بين 4-80 سنة استخدمت طريقة ال((En Vison لتحديد التعبير المناعي النسيجي ل P53,KI-67.النتائج :أظهرت النتائج إن هناك علاقة بين كل من العمر ونوع البولب مع زيادة في تعبير كل من المعلمين P53,KI-67 بينما لا توجد علاقة بين المعلمين ونوع الجنس .أظهرت الدراسة ان كلا المعلمين يرتبطان ببعضهما بعلاقة ايجابية.الاستنتاجات : من النتائج يمكن إن نستنتج إن العلاقة بين المعلمين P53,KI-67 وكل من العمر ونوع البولب يوكد إمكانية حدوث سرطان القولون مع ازدياد العمر وأيضا إمكانية حدوثه في النوعين الأكثر شيوعا هما Adenomatouse وHyperplastic اللذان تظهر بهما فرط التعبير في المعلمين P53,KI-67لذا فهما أكثر عرضة لحدوث سرطان القولون .التوصيات: الكشف المبكر لبولب القولون مع الإسراع باستئصاله مما يقلل إمكانية حدوث سرطان القولون وعلى المرضى الذين يعانون من البولب المتضاعف عليهم القيام بالمتابعة الصحية وإجراء الفحوصات باستمرار لمنع حدوث السرطان.

Keywords

Colonic Polyp --- Expression --- KI-67 --- P53.


Article
Protective Role Of Honey on The Dorsal Surface of The Tongue of Methotrexate Treated Rats (Histological and Immunohistochemical Study)

Authors: Ali Sultan Al-Refai --- Hassan Ali Al-Barazenchy --- Hanan Abdulla Abdulqader --- Ameera Kamal Khalil
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 3 Pages: 670 -678
Publisher: Babylon University جامعة بابل

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Abstract

The most common side effect of chemotherapy is oral mucositis and the effective treatment is considered important in cancer patient. This study was aimed to evaluate the effect of honey as a treatment for dorsaltongue mucositis caused by the methotrexate.Forty Albino rats were usedin the present study and grouped randomly into control and study groups(20 animals each). The rats in the control group were divided randomly into two groups: 10 animals were treated by distilled water and 10 animals were treated by (2.5gm/kg) honey two times daily for eight days using gavage needle, and a physiological salinewas intraperitoneally injected at day four for all the animals. In the study group, they were intraperitoneally injectedby60 mg/kg of methotrexate at day four and the animals were treated in the same way like control group. At day eight, all the animals were sacrificed;and a cross section fromthe tongue was removed for histopathological and immunohistochemical (Ki-67 and Bcl-2 immunolabeling) analysis.Honey can partially protect the tongue from methotrexate induced cytotoxicity, and attenuate the associated injury. Ki-67 immune expression was non significantly increased (p>0.05), but Bcl-2 immune expression was significantly increased in (p<0.05) in comparison with methotrexate treated group.Honeycan produce protection against methotrexate induced dorsal tongue mucositis.Flavonoids and phenolic compounds in honey can accelerate the healing process by its anti-oxidant and free radical scavenging properties.

Keywords

Honey --- Mucositis --- Chemotherapy --- ki-67 --- Bcl-2

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