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Article
PREPARATION OF C-REACTIVE PROTEIN LATEX REAGENT OF HUMAN SERUM
تحضير محلول لاتكس لبروتين Cالمنفعل من مصل الإنسان

Authors: سحر عبد الوهاب الشالجي --- ليلى خالد مهدي --- منى صبار
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2006 Volume: 5 Issue: 1 Pages: 112-122
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACTC-reactive protein (CRP) is an acute phase reactant the concentration ofwhich increases significantly following tissue injury or inflammation. It ispresent in the albumin fraction of the serum of patients and is antigenic uponinjection into rabbits. We report here the development of a local latex reagentcoated with rabbit anti-CRP. For this purpose CRP was isolated from pooledsera of patients and injected into rabbits. CRP rabbit antiserum was purified byusing ammonium sulfate precipitation. Followed by passing on ion exchangechromatography (Diethylaminoethyl "DEAE" cellulose). Then, latex particleswere coated with purified antisera. The prepared CRP latex reagentagglutination was observed upon the addition of patients' sera and comparedwith that of normal sera. In addition, it was evaluated against a standard CRPlatex reagent. The prepared reagent gave positive results (100%) with patients'sera and the positive control of the standard kit. While, the negative control ofthe standard kit and 30% of the normal sera gave negative results.

الخلاصةاحد بروتينات الطور الحاد الذي يزداد تركيزه بشكل ملحوظ بعد اصابة C يعد البروتين المنفعلالنسيج والالتهاب. يوجد هذا البروتين في جزء الالبومين من مصل المرضى وذو فعالية مستضدية عندC حقنة في الارنب. تم في هذا البحث تطوير محلول لاتكس محلي مغلف بالاضداد النوعية لبروتينمن خليط امصال مرضى و تم C المنفعل المحضر في الارنب ولهذا الغرض تم عزل البروتين المنفعلالمحضرة في الارنب C حقنة في الارنب, و من ثم تمت تنقية الاضداد النوعية للبروتين المنفعلبعد ذلك تم تغليف محلول الاتكس ,DEAE باستخدام الترسيب بكبريتات الامونيوم ثم بالمبادل اللايونيبالاضداد النوعية النقبة. لوحظ حدوث التلازن مع محلول الاتكس المحضر بعد اضافة مصل المرضى لهبالمقارنة مع المصل الطبيعي. قييست بعد ذلك العدة المحضرة مع محلول لاتكس قياسي, اذ اعطىالمحلول المحضر محليا تفاعلا ايجابيا مع امصال المرضى والسيطرة الايجابية للعدة القياسية بنسبة%100 بينما اعطت تفاعلا سلبيا مع امصال المرضى بنسبة 30 % وكذلك مع السيطرة السالبة للعدةالقياسية.PDF created


Article
Early Detection of Bacteremia and Bacteriuria after Extracorporeal Shock Wave Lithotripsy By C-Reactive Protein Measurements

Author: Ehab Jasim Mohammad
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2017 Volume: 16 Issue: 1 Pages: 41-46
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT :BACKGROUND:Extracorporeal shock wave lithotripsy (ESWL) has long been accepted as the least invasive and reliable method in the treatment of urolithiasis. The success rates have been very high in renal and upper ureteral stones . OBJECTIVE:Is early detection of bacteriuria and bacteriemia after extracorporeal shock wave lithotripsy (ESWL) of calcium and infection stones by measurement of plasma C-reactive protein (CRP) levels.METHODS: A total of 150 patients who had infection stones (n 54) and calcium stones (n 96) were included in the study. All patients had sterile urine before ESWL. The mean age was 41.6+_ 4.85 and male/female ratio was 2.12. Blood cultures were obtained within 1 hour post-ESWL period. Urine cultures were obtained 3 times just after and on the first and seventh day of ESWL. RESULTS: Post-ESWL evaluations showed 6 positive blood cultures with 4(5.32%) patients in infection stone and 2 (2.66%) patient in calcium stone groups, whereas urine cultures revealed 12 (16%) positive results in infection stones and 8(10..66%) in calcium stones. The patients who had positive cultures also had elevated plasma CRP levels when compared to the levels in patients with negative cultures (p 0.000). CONCLUSION: Bacteriuria and bacteriemia after ESWL have been well-identified entities and may be responsible from some of the post-ESWL complications. CRP can be useful for early detection of such complications.


Article
Elevation Salivary C - Reactive Protein (CRP) Levels in Iraqi Women with Polycystic ovary syndrome (PCOS ) and Oral diseases

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Abstract

Background: Polycystic ovary syndrome (PCOS) is the most commonendocraniopathy in women of reproductive age, affecting (5-10) % of populationand it is the leading cause of female infertility.C-reactive protein CRP, is a member of a group of acute phase of protein whichincrease their concentrations during certain inflammation disorders and used as abiomarker of inflammation in the body.Aim of the study: is to assess the salivary level of CRP with were oral disease wasstimulated by evaluate gingival inflammation, plaque accumulation in some Iraqiwomen with PCOS disease in compared with women not have PCOS.Methods: 19 females with PCOS and 26 females with systemically healthy include inthis study. Saliva were collected and CRP were examined and evaluated by usingELISA method in both study and control group. For all groups we examine theoral inflammation was measured depending on gingival and plaque index.Results: the results reported all PCOS women have significant increase in the salivaryCRP than women with no PCOS) mean ± SD (226.8±34SD, 173.8±22.5respectively). All women with PCOS had significantly increased in gingivitis thanwith no PCOS women.Conclusion:An increase in salivary CRP and gingival inflammation were found in womenwith PCOS. Also suggested measurement of salivary CRP may be helpful inpredicting women with oral disease in PCOS women.

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