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Article
Smoking and its relation to caries experience and salivary lactobacilli count

Author: Athra M. Al-Weheb عذراء الوهب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2005 Volume: 17 Issue: 1 Pages: 92-95
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Smoking has many harmful effects on the body. The aim of this studyis to investigate the effect of cigarettes smoking on count of lactobacilli ,the dental caries, and salivary factors.Subjects and Methods: 15 male smokers and 15 male non-smokers aged(24-29) years were chosen from post graduate students in College of Dentistry, they were interviewed about smoking behavior.Stimulated salivary sample was analyzed for lactobacilli count ,salivary flow rate and salivary pH was determined.Results: Measurment of dental caries was expressed by DMFT/DMFS.Results There was a significant relation between lactobacilli and DMFT/DMFS in smokers group with no significant differences concerning salivary flow rate and salivary pH between the two groups .Conclusion: Long term use of tobacco smoking affect the dental caries but not the salivary flow rate or salivary pH


Article
Assessment of Body Conformation For Carcasses of Broiler Exposure With Lactobacilli Bacteria 2-Body Measurement
تقييم القياسات الجسمية لذبائح فروج اللحم المجرع ببكتريا العصيات اللبنية (Lactobacilli) 2: قياسات الجسم

Author: Bushra . S . R . Zangana بشرى سعدي رسول زنكنة
Journal: Tikrit Journal for Agricultural Sciences مجلة تكريت للعلوم الزراعية ISSN: 18131646 Year: 2017 Volume: 17 Issue: 1 Pages: 111-118
Publisher: Tikrit University جامعة تكريت

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Abstract

This experiment was conducted in order to assessment of body measurement for carcasses of broiler exposure to Lactobacilli bacteria at the age of 1 , 21 days . A total of 90 broiler chicks of ross strain one day old were used and distributed to three treatments , 30 chicks for each treatment , the chicks of each treatment were divided in to two replicates(15chicks for each replicate).The treatments were as follows : (T1)group of chicks treated with Lactobacillus bacteria at 1day (T2)group of chicks treated with Lactobacillus bacteria at 21 day (T3)control group . The chicks were treated with bacteria by oral , the decided doze was counted on the basis of saving no less than 106 colony forming units(CFU) of Lactobacilli bacteria for each chick . Results revealed the following :- The microbial exposure results to lactobacilli bacteria (T1,T2) indicated a significant (P<0.05) improvement in the circumambient of breast , flier repletion of body , long of sternum , body depth at medium and end of sternum and meat weight of main cuts , also improve in body length , circumambient of thigh , flier repletion of breast , long of bones , body depth beginning of sternum and meat yield for main cuts of broiler carcass compared with control group (T3) . No significant effect of microbial exposure observed in relative weight of meat , bon and percentage meat : bon for main cuts of broiler carcass reard to old 32 days compared with control treatment (T3) .

استهدفت الدراسة تقييم القياسات الجسمية لذبائح فروج اللحم والمربى لعمر 35 يوم والمجرع فمويا ببكتريا العصيات اللبنية عند عمر1و21 يوم ، حيث تم استخدام 90 فرخ من فروج اللحم بعمر يوم واحد وزعت الافراخ على ثلاثة معاملات بواقع 30 فرخ/معاملة وقسمت افراخ كل معاملة الى مكررين (15فرخ/مكرر) وكانت المعاملات على النحو الاتي: المعاملة الاولى(T1) مجموعة الافراخ التي جرعت ببكتريا العصيات اللبنية بعمر1 يوم ، المعاملة الثانية(T2) مجموعة الافراخ التي جرعت بعمر21 يوم ، المعاملة الثالثة(T3) مجموعة افراخ السيطرة ، وكانت الافراخ تجرع يدويا بالبكتريا عن طريق الفم ، اذ حسبت الجرعة المقررة على اساس يوفر واحد مل مالايقل عن610 (CFU)Colony Forming Unite . في نهاية فترة التجربة والبالغة 35 يوم تم ذبح وتنظيف الذبائح وجرى حساب القياسات الجسمية والتي تشمل طول جسم الذبيحة ، محيط الصدر والفخذ والظهر ، درجة امتلاء الجسم والصدر، طول العظام وعمق الجسم ، وقد اظهرت النتائج ان التجريع المايكروبي ببكتريا العصيات اللبنية للمعاملتين(T1 وT2) ادى الى تفوق معنوي(P<0.05) في قيم كل من صفة محيط الصدر ودرجة امتلاء الجسم وطول عظم القص وعمق الجسم من بداية عظم القص ، فضلا عن تحسن في صفة كل من طول الجسم ومحيط الفخذ ودرجة امتلاء الصدر وطول عظمي الفخذ والوصلة الفخذية وعمق الجسم من وسط ونهاية عظم القص وكمية حاصل اللحم للقطع الرئيسية مقارنة بمعاملة السيطرة (T3) لذبائح فروج اللحم . لم يلاحظ وجود تأثير معنوي لمعاملتي التجريع المايكروبي (T1 وT2) في الوزن النسبي لكل من اللحم والعظم ونسبة اللحم: العظم للقطع الرئيسية ( الصدر ، الفخذ ، الوصلة الفخذية) لذبائح فروج اللحم مقارنة بمعاملة السيطرة (T3) .


Article
Capability of Lactobacillus acidophilus supernatant to inhibit production of lipase from methicillin-resistant Staphylococcus aureus
على تثبیط انتاج اللایبیز من العنقودیات الذهبیة المقاومة Lactobacillus acidophilus مقدرة طافي للمیثیسیلین.

Authors: Harith Jabbar Fahad حارث جبار فهد --- Hala Muayad Radeef حلا مؤید ردیف
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN: 19918941 Year: 2011 Volume: 5 Issue: 2 Pages: 1-5
Publisher: University of Anbar جامعة الانبار

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Abstract

The ability of acid supernatants of Lactobacillus acidophilus isolated from a vinegar weretested against methicillin resistant Staphylococcus aureus (MRSA) with lipolytic activity isolated from acnepimple. Results revealed a significant effect of cell free supernatant on MRSA isolates in comparison withcontrol. Furthermore, subinhibitory concentrations of acid supernatants found to be very effective ininhibiting the production of lipase from biofilm and planktonic cells of MRSA isolates. What's more, acidsupernatants of L. acidophilus showed inhibiting effect on the crude lipase in egg yolk agar plates

المعزولة من الخل ضد العنقودیات الذهبیة المقاومة Lactobacillus acidophilus الخلاصة:اختبرت قدرة الطافي الحامضي لبكتریامقارنة مع MRSA المحللة للدهون المعزولة من حب الشباب. اظهرت النتائج تاثی ا ر معنویا للطافي في عزلات (MRSA) للمیثیسیلنالسیطرة. كما وجد ان الت ا ركیز تحت المثبطة للطافي الحامضي كانت مؤثرة جدا في تثبیط انتاج اللایبیز من الخلایا الهائمة و الغشاء الحیاتيفضلا عن ذلك امتد التأثیر التثبیطي الى اللایبیز الخام في اطباق مح البیض. .MRSA لعزلات


Article
Histopathological Effect of Lactobacilli on Murine Spleen

Author: Abed Hassan Baraaj
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 2 Pages: 284-288
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Lactobacilli are ubiquitous in the environment and in human body. They widely used as probiotic therapeutic agents. However, several authors reported that this bacteria may cause several serious infections in various parts of human body.OBJECTIVE:This work is aimed to illustrate the histopathological effect of lactobacilli in murine spleen which play a major role in the immune system.MATERIALS AND METHODS:Three Lactobacillus isolates (namely, L. bulgaricus, L. plantarum and L. acidophilus) were isolated form yogurt, vinegar and vagina, respectively. These isolates were injected intraperitoneally in mice in a dose 1.5 X 108 CFU/ml.RESULTS:Several histopathological changes were caused by lactobacilli represented by degeneration, necrosis, presence of haemosidrin and macrophages and lymphoblasts.CONCLUSION:This non-pathogenic bacteria was able to cause severe damage to the spleen of mice.

Keywords

lactobacilli --- spleen --- red pulp --- white pulp


Article
Comparison between severe haemophilic A and healthy children in Streptococcus mutans, oral Lactobacilli and Candida albicans counts

Authors: Zainab A. AlDhaher زينب الظاهر --- Maha F. Almelan --- Luma M. Alhadi لمى الهادي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 149-153
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Haemophilia is a common hereditary hemorrhagic disorder, however little is known about the oralmicroflora of hemophilic patients.The purpose of this research is to compare between the viable count of Streptococcus mutans, oral Lactobacilli andCandida albicans from saliva of children suffering from haemophilia A aged (6-12) years and the viable count of thesame microorganisms isolated from saliva of healthy subject (healthy supject group) aged (6-12) years.Materials and methods: - Saliva samples were collected from 30 children with severe Haemophilia A (patients groupand 30 healthy children (healthy subject group). Microbial counts of Streptococcus mutans, oral Lactobacilli andCandida albicans were recorded for each group by using colony counter and expressed as colony forming unitmultiplied by the dilution factor per millimeter saliva (CFU/ml).Results: - The present study observed that the viable count of Streptococcus mutans and oral Lactobacilli in patientgroup was higher than the count of the healthy subject group while no significant differences were observedbetween the viable count of Candida albicans in patients group and healthy subject group.Conclusion: - education, prevention and effort among parents and dental professionals can aid in improving the oralhealth of Haemophilia children


Article
Balance of Vaginal System
توازن نظام المهبل

Author: Zahra M. Al-Khafaji , Khairiya Jaber
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2013 Volume: 12 Issue: 1 Pages: 1-21
Publisher: Baghdad University جامعة بغداد

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Abstract

In general the success of biosystem depends on its flora . Human represents superorganism as it contains variable microbial flora which called human microbiome. The latter divided into core microbiome and variable microbiome . Distribution of human microbiome concentrated in systems and organs exposed to the outer environments, such as ; skin, mouth , nose, digestive channel , urogenital channel .Vagina represents one of the secondary environment in the body responsible for reproductive health . and since it exposes to the external environment it should have some sorts of defenses . The components of vaginal defenses are: vaginal epithelial cells and its associates which excretes some defensins. Vagina has its own innate immunity, presence of IgG, IgA. In the vagina, there is some enzymes or enzyme inhibitors such as Stable serine protease inhibitor (SLPI) . In addition there are some more defense facilities such as Mannose binding lectins (MBL) , Chaperone (HSPs) which increased upon infections and inflammations , antimicrobial peptides. The main natural flora of the vagina is Lactobacilli, which classified into Lactobacillus acidophilus complex using routine rout and can be differentiated more using molecular route . These bacteria are responsible for setting the acidic reaction of the vagina . It can protects vagina against different infections and can be used to restore the unbalanced vaginal conditions if it selected carefully according to certain criteria .

الخلاصة: ان مكنون الانسان الميكروبي يمثل مجموع التراكيب الوراثية للأحياء المجهرية المختلفة التي يحويها والمنتشرة في الاجهزة والاعضاء المعرضة الى البيئات التي يعيش فيها الانسان مثل :الجلد والفم والقناة الهضمية والقناة البولية التناسلية. ويعتبر المهبل واحدا من البيئات الثانوية في الجسم ويكون مسؤولا عن صحة الجهاز التناسلي. ومن مكونات الجهاز الدفاعي للمهبل خلايا بطانة الرحم، وتنتج هذه الخلايا موادا مضادة للميكروبات مما يساعد المهبل على تقوية دفاعاته الذاتية، وكذلك الانزيمات الموجودة في المهبل وانتاج مثبط البروتيزات السيرينية (SLPI) وبروتين رابط للمانوز (MBL) وغيرها. ان اهم البكتريات الموجودة في الرحم هي بكتريا Lactobacilli وهذه البكتريا مسؤولة على الحفاظ على الوسط الحامضي للمهبل، وهي تحمي المهبل من الاصابات الجرثومية.


Article
Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells
تأثير مصدر بكتريا Lactobacilli في التصاق بكتريا Escherichia coli و Staphylococcus aureus على الخلايا الطلائية البولية

Authors: Alaa' Mohammed Hasan الاء محمد حسن --- Harith J. F. Al-Mathkhury حارث جبار فهد المذخوري
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2011 Volume: 8 Issue: 3 Pages: 723-727
Publisher: Baghdad University جامعة بغداد

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Abstract

The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fermentum and L. plantarum) were more competitive than food lactobacilli (L. acidophilus and L. bulgaricus).

اختبر الفعل التثبيطي لأربع عزلات بكتريا حامض اللاكتيك وهي L. bulgaricus و L. acidophilus و L. plantarum و L. fermentum عزلت من اربعة مصادر مختلفة و هي اللبن و الخل و اللعاب و المهبل على التوالي لمنع التصاق E. coli و S. aureus بالخلايا الطلائية البولية. اظهرت النتائج ان عزلات Lactobacillus جميعها او طافي الزرع استطاعت خفض اعداد الممرضات البولية الملتصقة على الخلايا الطلائية البولية. في حين كان مستوى التثبيط بالنسبة لخلايا Lactobacillus اكثر من الطافي للخلايا نفسها. و من ناحية اخرى كانت بكتريا حامض اللاكتيك المعزولة من مصادر بشرية (L. fermentum و L. plantarum ) قادرة على التنافس أكثر من تلك المعزولة من الاغذية (L. acidophilus و L. bulgaricus).


Article
Correlation between caries related bacteria in plaque and saliva in different age group children

Authors: Zainab J. Ja’far زينب جعفر --- Yasameen A.A. Al-Bayati ياسمين البياتي --- Ghada I. Taha غادة طه
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 140-144
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental plaque contains bacteria that are both acidogenic and acidoduric. Different types ofstreptococci were identified in saliva. Although many bacterial subspecies have been shown to be associated withcaries, streptococcus mutans is still believed to be the most important bacterium in the initiation of this disease whilelactobacillus is correlated with the active caries episode. This study was conducted in order to estimate thecorrelation between caries related bacteria in plaque and saliva in different age group children.Materials and methods: Fifty three children aged 3-10 years old were chosen for this study. Recording of dental carieswas carried out by the dmfs index for primary teeth and DMFS index for the permanent teeth according to thecriteria suggested by the WHO. One ml of unstimulated (resting) whole saliva was collected from the children usingspitting method then diluted and applied on the surface of agar media specific for streptococcus mutans andlactobacilli growth. Dental plaque sample was taken from the buccal surface of the maxillary second primary molarby a clean toothpick and store in Epindorf tube which contain 1 ml. normal saline then inoculated in the samegrowth media that were used with the salivary samples. Colonies of the bacteria were counted with the aid ofdissection microscope (15 X) on the basis of their characteristic morphology.Results: Strong positive significant correlation was found between dmfs and ds components of the primary teeth,Positive results was found when correlating dmfs or ds with streptococcus mutans in dental plague while a negativecorrelation was found with lactobacilli. Negative correlation was found when correlating dmfs with streptococcusmutans in saliva while the relation is positive with lactobacilli. In dental plaque and in saliva there was a strongpositive highly significant correlation between the DMF and the DS. Correlation coefficient between DMFS with thebacterial counts of the caries related microorganisms (streptococci and lactobacilli) in the dental plaque and insaliva revealed weak, negative not significant correlations.Conclusion: The relation is not significant between the caries related microorganisms with each other in differentmedia; either the dental plaque or saliva


Article
Dental caries, Mutans Streptococci, Lactobacilli and salivary status of type1 diabetic mellitus patients aged 18- 22 years in relation to Glycated Haemoglobin

Authors: Juman D. Al- Khayoun جمان الخيون --- Ban S. Diab بان صالح ذياب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 1 Pages: 153-158
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: diabetic mellitus is one of the serious systemic diseases that may cause general systemic changes,which may be reflected in the oral cavity. The aims of this study were to assess the severity of dental caries, MutansStreptococci and Lactobacilli in addition to flow rate and pH among uncontrolled and controlled diabetic groups incomparison with non-diabetic control group.Materials and Methods: Study groups consisted of 25 uncontrolled diabetic patients (HbA1c > 7), 25 controlleddiabetic patients (HbA1c ≤ 7), in addition to 25 non-diabetic healthy looking individuals. Their age was (18-22) yearsfrom both genders. The diagnosis and recording of dental caries was according to severity of dental caries lesionthrough the application of D1_4MFS (Manji et al, 1989) and stimulated salivary samples were collected. Salivary flowrate and pH were estimated. Viable count of mutans streptococci (on Mitis- Salivarius Bacitracin Agar) andlactobacilli (on Rogosa) was determined.Results: The mean values of caries-severity were recorded to be highest among study groups compared to thecontrol with statistically highly significant difference (p<0.01). Lowest values of salivary pH and flow rate were amongstudy groups compared to the control with highly significant difference (p<0.01). Concerning Mutans Streptococciand Lactobacilli were found that the mean values of them for uncontrolled diabetic group were highly significanthigher than both mean values of controlled diabetic group and control group.Conclusion: Dental caries revealed higher percentage of occurrence and severity among uncontrolled diabeticgroup. Furthermore there was significant influence of the diabetic and the poor metabolic control on the salivaryflow rat, pH, mutans streptococci and Lactobacilli that have an effect on caries occurrence and severity


Article
ASSESSMENT OF QUALITY PROPERTS OF CARCASSES AND INTESTINAL MICROBIAL GUT OF BROILER EXPOSURE TO LACTOBACILLI BACTERIA
تقييم الخصائص النوعية للذبائح والنبيت المعوي للقناة الهضمية لفروج اللحم المجرع ببكتريا العصيات اللبنية (Lactobacilli) 1: قطعيات الذبيحة

Author: بشرى سعدي رسول زنكنة
Journal: Tikrit Journal for Agricultural Sciences مجلة تكريت للعلوم الزراعية ISSN: 18131646 Year: 2013 Volume: 13 Issue: 2 Pages: 92-97
Publisher: Tikrit University جامعة تكريت

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Abstract

This experiment was conducted in order to assessment of quality characteristics for carcasses and intestinal microbial of broiler exposure to Lactobacilli bacteria at the age of 1 , 21 days . Atotal of 90 broiler chicks of Ross strain one day old were used and distributed to three treatments , 30 chicks for each treatment , the chicks of each treatment were divided in to two replicates (15chicks for each replicate) . The treatments were as follows : the first and second treatment (T1,T2) is the group of chicks treated with Lactobacillus bacteria at 1 and 21 day old respectively , the three treatment (T3) is the control group . The chicks were treated with bacteria by oral , the decided doze was counted on the basis of saving no less than 106 colony forming units(CFU) of Lactobacilli bacteria for each chick . Results revealed the following The microbial exposure treatment(T1) have shown significant increase (P<0.05)in the body weight and carcass weigh of broiler at age 35 days , the everage of live body and carcass weight were1735.00g compared with 1462.5 and 1432.50g respectively with preced percentage 15.71 , 17.44% for body weight and 17.57 , 20.95% for carcass weight respectively also improved dressing percentages compared with control group . Microbial exposure treatment (T1,T2) had no effect on relative weight of carcass cuts percentages and edible internal organs without liver compared with control group (T3) The bacterial treatments (T1,T2) improved significant (P<0.05) over the ontrol treatment (T3) increased the counts of Lactobacillus bacteria in the crop , cecal and decreasing the counts of coliform bacteria in the crop , jejunum and cecal region compared to control group (T3) at the end of experiment .The result indicated that microbial exposure by oral were the best for performance .

استهدفت الدراسة تقييم الخصائص النوعية والنبيت المعوي لذبائح فروج اللحم بعمر 35 يوم والمجرع فمويآ ببكتريا العصيات اللبنية عند عمر1و21 يوم ، اذ استخدام 90 فرخ من فروج اللحم بعمر يوم واحد وزعت الافراخ على ثلاثة معاملات بواقع 30 فرخ/معاملة وقسمت افراخ كل معاملة الى مكررين (15فرخ/مكرر) وكانت المعاملات على النحو الاتي: المعاملة الاولى(T1) مجموعة الافراخ التي عوملت ببكتريا العصيات اللبنية بعمر1 يوم ، المعاملة الثانية(T2) مجموعة الافراخ التي عوملت بعمر21 يوم ، المعاملة الثالثة(T3) مجموعة افراخ السيطرة ، وكانت الافراخ تعامل بالبكتريا عن طريق التجريع بالفم ، اذ حسبت الجرعة المقررة على اساس يوفر واحد مل مالايقل عن610 (CFU)Colony Forming Unite . وقد بينت النتائج مايلي :- اظهرت معاملة التعرض ببكتريا العصيات اللبنية(T1) تفوق معنوي(P<0.05) في وزن الجسم والذبيحة لفروج اللحم عند عمر35 يوم مقارنة بالمعالتين(T2 وT3)، فقد بلغت المعدلات1735.00 غم مقارنة بـ1462.5 و1432.50غم على التوالي وبنسبة تفوق 15.71 و17.44% لوزن الجسم و17.57 و20.95% لوزن الذبيحة على التوالي ، فضلا عن تحسن نسبة التصافي مع الاحشاء الداخلية الماكولة وبدونها للمعاملتين(T1 وT2) مقارنة بمعاملة السيطرة(T3) لفروج اللحم. لم يلاحظ وجود تأثير معنوي للمعاملات المايكروبيه(T1 وT2) في الوزن النسبي للقطعيات الرئيسية والثانوية والاحشاء الداخلية الماكولة باستثناء الكبد مقارنة مع معاملة السيطرة (T3) . تفوقت معاملات التعرض المايكروبي (T1 وT2) ببكتريا العصيات اللبنية معنويا(P<0.05) وحسابيآ في زيادة معدل اعداد بكتريا العصيات اللبنية في منطقة الحوصلة والاعورين وانخفاض معدل اعداد بكتريا القولون معنويآ(P<0.05) في مناطق الحوصلة والصائم والاعورين على معاملة السيطرة(T3) لفروج اللحم نهاية فترة التجربة . يستنتج من هذه الدراسة ان التعرض المايكروبي ببكتريا العصيات اللبنية من خلال التجريع الفموي كان افضل في صفات وزن الجسم الحي ووزن الذبيحة ونسب التصافي والقطعيات الرئيسية لفروج اللحم المربى لعمر35 يوم .

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