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Article
Evaluation of Significance of Prolonged Liquefaction Time of Semen in Hypofertile Men

Author: Siddeek B Mar'ie
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 3 Pages: 328-333
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Until now most of the references adopt a sixty-minute liquefaction time as a guideline for normality in general semen analysis. During the last twenty years of interest in these patients, we found that there is a correlation between primary hypofertility, prolonged liquefaction time and therefore delayed conception worthy for this study. This study represent a clinical biometric case series on (284) patients consulting an outpatient urology clinic with primary hypofertility during the last ten years in Mosul.OBJECTIVE: To signify the impact of prolonged liquefaction time of semen on fertility potential of primary hypofertile men, including its relation with the delay of conception and other semen parameters.PATIENTS AND METHODS: Married males who are sexually healthy supposed to have healthy partners with no pregnancy who were not using any method of contraception. Patients should give their semen for analysis in the laboratory by masturbation. All patients should have at least two separate samples to be analyzed, with not less than 7 days apart. Collected data included age in years and duration of delayed conception in months. Semen parameters collected were: volume in milliliters (ml), liquefaction time in minutes, concentration (density) in millions/milliliters, motility in percentage, and morphology in percentage.RESULTS: The mean age of people in our sample was 29.7 years, mean volume of semen was 2.9 ml, mean liquefaction time was 26 minutes, the mean sperm density was 32.9 millions/ml, while the mean activity percentage was 31.2%, the mean percentage of the normal sperms was 61.9% and the mean duration of delay in conception was 33.7 months. There was a very highly significant correlation of prolonged liquefaction time with impaired motility, and morphology, and a significant correlation with sperm concentration. Also there was a linear positive relationship between prolonged LT in minutes and delayed conception in months, but it was more prominent in those hypofertile men who failed to conceive for ≥ 36 months.CONCLUSION: The prolonged liquefaction time has a possible role as a cause of delay in conception in hypofertile men, and has a significant relationship to defects in other semen parameters.


Article
The study of Oxidative stress, Sperm chromatin abnormality and seminal parameters in different groups for infertile patients
دراسة الجهد التاكسدي وكروماتين النطف غير السوي والمعالم المنوية لمجاميع مختلفة من مرضى العقم

Authors: Mohsin K. AL-Murshdi محسن كاظم المرشدي --- Yehya K. AL-Sultani يحيى السلطاني
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2016 Volume: 8 Issue: 3 Pages: 425-433
Publisher: University of Kufa جامعة الكوفة

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Abstract

The study was carried out between January 2014 and October 2014 including one hundred and fifty five infertile men who attended the Fertility center in AL-Sadr Medical City. The Objective of this study was to study of oxidative stress, sperm chromatin abnormality and semen quality including ( semen volume, Liquefaction time, sperm concentration, motility, and normal morphology. The results of the study showed that there was a significant increase (p<0.05) in the semen volume, sperm concentration, progressive motility and normal morphology for unexplained compared to five abnormal semen groups. Also it was a significantly decrease (p<0.05) in liquefaction time, leukocyte concentration, MDA concentration and sperm chromatin abnormality for unexplained compared with abnormal semen groups. This study supports the conclusion that oxidative stress in seminal plasma is the main causes of sperm chromatin defect with defect of semen parameters and related with male infertility.

اجريت هذه الدراسة بين شهر كانون الثاني لسنة 2014 وتشرين الاول لسنة 2014 وشملت مائة وخمسة وخمسون من الرجال العقيمين الذين راجعو مركز العقم في مدينة الصدر الطبية . هدفت الدراسة الى معرفة العلاقة بين الجهد التاكسدي وكروماتين النطف الغير السوي ومعالم المني المتضمنة ( حجم المني و زمن الاماعة وتركيز النطف والحركة التقدمية للنطف والشكل السوي للنطف). اظهرت نتائج هذه الدراسة زيادة معنوية (p<0.05) في حجم المني وتركيز النطف والحركة التقدمية للنطف والشكل السوي للنطف لمجموعة العقم غير المفسر مقارنة بخمسة مجاميع اخرى من العقم ، كذلك اظهرت نتائج الدراسة انخفاضا معنويا ( p<0.05) في زمن الاماعة وتركيز كريات الدم البيض وتركيز الـ MDA ونضوجية كروماتين النطف غير السوي لمرضى العقم غير المفسر مقارنة بالمجاميع الاخرى من العقم . استنتجت هذه الدراسة بان الجهد التاكسدي هو سبب رئيسي لضرر كروماتين النطف وكذلك ضرر معالم المني مما يؤدي الى حصول العقم .

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