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Article
Prevalence of Pseudomonas aeruginosa producing Metallo-β- lactamases in wounds and burns infections

Authors: Sabah Hasan Rhadi --- Intidhaar N.Abid
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2017 Volume: 6 Issue: 3 Pages: 58-67
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Metallo β -lactamases (MBLs) producing Pseudomonas aeruginosa have been detected from clinical isolates in worldwide with increase in emergence in the last years . The spread of MBLs positive isolates in a localized hospital environment causes not only a therapeutic problem but as well as a serious concern for infection control handling , hence , this study was aimed to determine the prevalence of P. aeruginosa producing MBLs isolated from some skin infections (wounds and burns). A total of 57 P. aeruginosa were isolated from wounds and burns infections (24 wound swabs and 33 burn swabs) in Al-Hussain Teaching Hospital .Primary screening of carbapenems resistant isolates appeared that 63% (36 isolates) were resistant to imipenem and meropenem .Among the 36 carbapenems resistant isolates that were tested for production MBLs by phenotypic test (CDT)with EDTA inhibitor (as chelating factor ), 34 (94%) were MBLs positive and 59.6% from 57 isolates were positive to MBLs production . MICs values of MBLs producers were higher in imipenem ((≥16-64) μg/ml)) than that in meropenem (( ≥16-32) μg/ml)) . Out of 34 MBLs producer , 24 (72.7%) isolates were founded in specimens of burn swabs , while the wound swabs specimens registered 41.6% . MBLs producing isolates were also tested for antibiotics susceptibility , all isolates (100%)were sensitive to aztreonam and 70.5% to ciprofloxacin . 100% of isolates were resistant to cefoxitin and amoxycillin – clavulanic acid.


Article
Bacteriological Study of Escherichia coli Isolated from Different Infections in Diyala
دراسة بكتريولوجية لبكتريا E. coli المعزولة من أخماج مختلفة في مدينة ديالى

Authors: Eman Abass Ali أيمان عباس علي --- Ibtihal Hameed Mohsin أبتهال حميد محسن --- Izdehar M. Jasim ازدهار محمد جاسم
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2017 Volume: 13 Issue: 3 - part 2 Pages: 249-261
Publisher: Diyala University جامعة ديالى

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Abstract

The goal of present study was to Isola and Identifi--cation of "Escherichia coli" from different Infections and detection the sensitivity and resistance of Ecoli to antimicrobials Additionally detection of virulence factors . This study was conducted from the period from 1 / 6 / 2016 to 1/ 9 / 2016 in Baquba city in Iraq.. It included; Fiftin samples were collected from different infections from Baquba General Hospital and AL-Batool Hospital. Twenty isolates were found to be Escherichia coli .The susceptibility test was applied on these isolates against deferent antibiotics. The results revealed that the highest resistances were for Piperacilline(93.3%) and highest Sensitive Imipenem with 100%, While the lowest resistance were for Tobramycin (9%) . Moreover the results of virulence factors that had E. coli showed possession of all isolates many virulence factors and a high production of which increases the pathoginicity of it. All isolates were unable to produce urease and gelatinase, but heamolycin (35%) . As well as, Tow isolate (10%) were able to production Extended Spectrumβ-Lactamases enzyme. Furthermore, four isolate (20%) were able to production metalloβ-Lactamases Finally, six isolate(30%) were able to production Bacteriocin.Conclusion: E. coli isolates highest resistances were for Piperacilline and highest Sensitive Imipenem with 100%, While the lowest resistance were for Tobramycin . additionally some of isolates production Extended Spectrumβ-Lactamases enzyme, metallo β-Lactamases and Bacteriocin.

الهدف من الدراسة الحالية هو عزل وتشخيص بكتريا الايشريكيا القولونية من أخماج مختلفة وكشف حساسية ومقاومة هذه البكتريا للعوامل الضد مايكروبية ,بالاضافة الى الكشف عن عوامل الضراوة .جمعت النماذج للفترة من1/6/2016 ولغاية 1/9/2016 في مدينة بعقوبة في العراق.وتضمنت:جمع 50 عينة من أخماج مختلفة من مستشفى بعقوبة التعليمي ومستشفى البتول.20 عزلة كانت بكتريا الايشريكيا القولونية .كما أجري أختبار الحساسية للعزلات تجاه مضادات حياتية مختلفة .أظهرت النتائج أن أعلى مقاومة كانت تجاه المضاد الببراسيلين وبنسبة (93.3%) ,وأعلى حساسية للمضاد الاميبينيم وبنسبة(100%) بينما أقل مقاومة للمضاد التوبرامايسين وبنسبة (9%).كما أوضحت نتائج عوامل الضراوة لبكتريا الايشريكيا القولونية امتلاك كل العزلات لعوامل الضراوة والانتاج العالي يزيد من امراضيتها. كما ان كل العزلات منتجة لانزيمي اليوريز والجيلاتينيز ,أما أنتاجيتها للهيمولايسين فقد كان بنسبة (35%) .بالاضافة الى ذلك عزلتان (10%) كانت منتجة لانزيمات البيتالاكتاميز واسعة الطيف. وأربع عزلات وبنسبة (20%)قادرة على أنتاج أنزيمات البيتالاكتاميز المعدنية,وأخيرا"ست عزلات وبنسبة (30%) منتجة للبكتريوسين. الاستنتاجات: كانت لعزلات بكتريا الايشريكيا القولونية مقاومة عالية تجاه المضاد الببراسلين وأعلى حساسية تجاه المضاد الاميبنيم وبنسبة 100%بينما أقل مقاومة كانت تجاه المضاد التوبرامايسين .بالاضافة الى ذلك كانت بعض العزلات منتجة لانزيمات البيتالاكتاميز واسعة الطيف ,وانزيمات البيتا لاكتاميز المعدنية والبكتريوسين.

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