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MICROSPECTROPHOTOMETRIC QUANTIFICATION OF THE SKELETAL MUSCLE GLYCOGEN CONTENTS WITH AGING

Author: Huda R Kareem هدى رشيد كريم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 1 Pages: 27-35
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundSkeletal muscle fibers contain about 2% of its weight is glycogen, this glycogen used to keep the muscle functioning if it fails to receive sufficient oxygen. PAS stain is useful in detecting cytoplasmic accumulation of glycogen. Glycogen had been studied in skeletal muscles under various state of muscle activities and nutritional states but glycogen quantification with aging is not clearly defined till now.ObjectivesQuantification of the mean glycogen concentration in skeletal muscles fibers stained with PAS stain in various age groups by microspectrophotometry.MethodsThe tibialis anterior muscle of 20 Albino male rats (rattus rattus norvegious) of neonate, 3, 6, 9, 12, and 18- months were selected. Paraffin blocks were performed, sectioned and stained with PAS stain.Analysis of the PAS stained sections by microspectrophotometry at 510 nm wave length. For the test group, mean absorbance, standard deviation, maximum, minimum, and mode values were estimated and compared with the control groups.ResultsA significant difference in PAS absorbtion between test and control groups, and among different age groups, being increased with age.ConclusionThe variation in PAS absorbtion with aging indicates that the glycogen content in skeletal muscle increase with aging, this could be due to the influence of age on skeletal muscle glucose transport and glycogen metabolism.Key wordsSkeletal muscle, PAS, Glycogen, Microspectrophotometry


Article
DNA Quantitation in Pediatric Acute Leukemia

Authors: Najiha A. Ameen --- Safa A. Faraj --- Hasanein H. Ghali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2015 Volume: 8 Issue: 2 Pages: 2229-2234
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Deoxyribose Nucleic Acid (DNA) ploidy, and immunophenotyping are now established as prognostic markers, however provide vulnerable information regarding long term outcome of acute leukemia.Aims of study: To establish a histochemical quantitation of nuclear DNA content of acute leukemia patients by Microspectrophotometry (MSP).Patients and methods: Prospective study on (55) patients with newly diagnosed acute leukemia from Children Welfare Teaching Hospital / Medical City Complex during the period from October 2002 to June 2003 and (10) patients with viral lymphocytosis as control group. MSP technique was used to determine the DNA content in leukemia blasts nuclei. Data were tabulated using SPSS (Statistical package for the social sciences) version 18.0 for windows. P-values equal or less than 0.05 were considered significant.Results: DNA cytochemical quantitation was studied after applying Feulgen stain. The amount of DNA per nucleus were expressed as means optical density × 10-3. The results showed that the mean DNA value was (0.545) while that of control group was (0.418). The ALL group was shown to have significantly higher mean OD compared to other groups.The frequency distribution of the different groups shows that there is homogeneous population of the mean OD of Feulgen stained nuclei for the control, AML, and AUL groups while it shows a heterogeneous population for ALL group. In comparison with the range of OD of nuclei of the control group, leukemia cases can be differentiated into diploid and aneuploidy classes. DNA aneuploidies were identified in (12/55) cases analyzed, thus accounting for (21.8%). For the ALL group, the mean OD readings in (18) patients (60%) were within the diploid region while (12) were outside the range (aneuploidy type) (40%).Conclusions: DNA quantitation determined by MSP may represent an additional factor to improve the definition of risk groups of acute leukemia, it will continue to be a valuable tool for understanding tumor growth heterogeneity.

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