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ELFA and IFAT Techniques to Detect Chlamydial Infections in Baghdad Women and Its Effect on the Immunoglobulins Level.

Authors: Khamael M. Kadaem* MSc --- Raad K. Al-Husseinei --- Lamya A. AL-Janabi --- Mun'im M. Fathei
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2009 Volume: 51 Issue: 2 Pages: 192-197
Publisher: Baghdad University جامعة بغداد


Background: Chlamydia trachomatis is one of the most common human pathogens and considered asone of the causative agents of STDs. This organism cause acute and recurrent pelvic infections andinfertility.Patients and Methods: Two hundred and seventy three females were included in the present study,attending infertility department, AL-Elwiya hospital, AL-Jarah private hospital, central public healthlaboratory and STDs clinic to whom IFAT, ELAF and immunoglobulins concentration were done.Results: Females were divided into three age groups <20; 20-39 and ≥40 years. Single and repeatedabortions were 44.9%, 55.1% respectively. Primary and secondary infertility were 55.6% and 44.4%.Higher abortions rate were in the age group 20-39 years which represents 31.5% and 41.6% in singleand repeated abortions. Primary and secondary infertility were high in the same age group whichrepresents 42.6% and 33.3% of the total investigated females, also the multipartners within the sameage group constitutes 56.7%.Chlamydial infections detected by IFAT technique constitute 12.8% whichwere represented as 14.6%, 11.1%, 36.7% and 5% in abortions, infertility, multipartners and fertilitywith no abortion groups respectively. While by ELFA technique the percentages were 12.4%, 9.3%,30% and 4% in the same mentioned groups.Immunoglobulins mean value in females with chlamydialinfections wee as follows IgG = 2102.1 mg/dl; IgA = 317.9 mg/dl and IgM = 272.5 mg/dl. Which weremore than the normal values of the immunoglobulins.Conclusions: Chlamydial infections were distributed largely among multipartners than other abortedfemales. High abortions were noted in age group 20-39 years. IFAT technique was more reliable thanELFA technique to detect chlamydial infections.IgG and IgM concentrations were higher than normal concentrations, while IgA remains normal.

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