research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
THE INFLUENCE OF N-RAS GENE MUTATIONS ON THE RESPONSE TO INDUCTION THERAPY IN AML IRAQI PATIENTS

Authors: Nahidh K. Alwan ناهض كامل علوان --- Raad J. Musa رعد جابر موسى --- Ban A. Abdul-Majeed بان عباس عبد المجيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 3 Pages: 230-237
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:N-RAS mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those of myeloid origin.Objective:Current study aimed to determine the frequency of N-RAS mutation; and its influence on response to induction therapy in patients with acute myelogenous leukemia (AML) in Iraq.Methods:Peripheral blood and bone marrow samples were taken from 58 newly diagnosed AML patients and 30 individuals with reactive bone marrow were selected as a control group. Samples screened for N-RAS gene mutations using nested PCR were followed by mutation sensitive digestion analysis (MSDA).Results:N-RAS mutations at the time of diagnosis were found in 10/58 (17.24%) patients with AML and no mutation in control individuals. Patients with mutant N-RAS showed lower complete remission (CR) than wild type, the difference was not significant (60% vs. 72.92%, P = 0.414).Conclusion:The current results provide clues for activation of RAS-signaling cascade in AML patients, supporting their role in molecular pathogenesis of leukemia. N-RAS mutations show no influence on CR rate in AML patients. Further studies on larger scale to define the prognostic significance of N-RAS mutations are recommended.Keyword:AML, N-RAS mutation, MSDA, complete remission.

Keywords

AML --- N-RAS mutation --- MSDA --- complete remission


Article
ANALYSIS OF N-RAS GENE MUTATIONS AND P21N-RAS PROTEIN EXPRESSION IN IRAQI PATIENTS WITH IN AML

Authors: Nahidh K. Alwan ناهض كامل علوان --- Raad J. Musa رعد جابر موسى --- Ban A. Abdul-Majeed بان عباس عبد المجيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 4 Pages: 329-339
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:N-RAS mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those of myeloid origin.Objective:Current study aimed to determine the frequency of N-RAS mutation and its correlation with P21N-RAS protein expression in patients with acute myelogenous leukemia (AML) in Iraq.Methods:Peripheral blood, bone marrow aspirate and biopsy samples were taken from 58 newly diagnosed AML patients (57 de novo and 1 therapy related AML) and 30 individuals with reactive bone marrow conditions were selected as a control group. Samples screened for N-RAS gene mutations using nested PCR followed by mutation sensitive digestion analysis (MSDA), and immunohistochemical analysis of P21N-RAS protein expression by using anti N-RAS monoclonal antibody.Results:N-RAS mutations at the time of diagnosis were found in 10/58 (17.24%) and P21N-RAS expression found in 5/58 (8.62%) patients with AML. There was a significant difference (P = 0.001) in P21N-RAS expression between mutant and wild type N-RAS patients with AML. No N-RAS mutations or P21N-RAS expression detected in the control group individuals.Conclusion:It can be suggested that there is activation of RAS-signaling cascade in AML patients, this is may support their role in molecular pathogenesis of acute leukemia. Also, there was a significant difference between N-RAS gene status and P21N-RAS protein expression in patients with AML.Keyword:AML, N-RAS Mutation, MSDA, P21N-RAS expression, Digital analysis.

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2013 (2)