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Article
A comparative Study to Evaluate the Effect of Sodium hypochlorite Temperature on Cyclic Fatigue Resistance of Three Types of Rotary Instrument Prof.

Authors: Dr. Ayad Mouayad Mahmood B.D.S, M.Sc. د. اياد مؤيد محمود --- Dr. Abdul-karim Jassim Al_Azzawi B.D.S, M.Sc. د. عبد الكريم جاسم العزاوي
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2013 Volume: 10 Issue: 2 Pages: 193-199
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: The fracture of instruments within root canal during endodontictreatment is a common incidence, fracture of fatigue through flexure occursbecause of metal fatigue, NaOCl used for irrigation may have an effect oncorrosion that decrease fracture resistance. This study aimed to assess the effect ofNaOCl temperature on the cyclic fatigue of different type of Endodontic NiTiRotary Instruments and compare among them.Materials and method: three types of rotary instruments with tip size 0.25: ProTaPerF2 (Densply,Malifier) Revo-S SU ( 0.06 taper, MicroMega) and RaCe system(0.06 taper, FKG,Dentaire), Thirty file of each instrument type were used withincanal with 60° angle of curvature , undiluted NaOCl (5.25% ) were used. Ten filewithout immersion in NaOCl, ten were immersed at room temperature (21°C) for5 minute and ten with immersion in NaOCl at 60°C for the same time. Nine groupwere formed for all instruments (total number=90). The testing canal customizedwithin stainless steel block covered with glass face, the time to fracture recordedand the mean of cycles to fracture (MCF) detected for each instrument .Data wereanalyzed statistically by ANOVA, LSD and Independent T-test at 5% significantlevel.Result: there was a highly significant difference in mean of cycles to fracture for eachrotary instrument when temperature of NaOCl increased that the Number of cyclesto fracture decreased as the temperature increased. RaCe revealed the best fractureresistance followed by ProTaper then Revo-s that showed the less resistance.Conclusion: the rotary instruments more prone to fracture when temperature ofNaOCl increased, as well as the rotary instruments differ from each otheraccording to manufacturing process, taper, cross section and other factors.


Article
Efficiency of diode laser and sodium hypochlorite as root canal disinfectant against Enterococcus Faecalis: An In vitro study

Authors: Mahmoud Y.M. Taha --- Nawfal A. Zakaria --- Nagwan F. Shehab
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 330-338
Publisher: Mosul University جامعة الموصل

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Abstract

Objectives: The main objective of the current in vitro study is to compare the antimicrobial efficiency of diode laser 1064nm at different output powers and times with sodium hypochlorite at different concentrations and times against E. faecalis when used as root canal disinfectant. Materials and methods: 140 human extracted single-rooted teeth were decoronated to a length 14mm from the apical foramen to the cervical border of the root, then adjusted working length to 13mm, autoclaved and inoculated with a suspension of E. faecalis at a concentration 4×105 cfu and incubated at 37˚C for 24 hours. Samples were divided into 14 groups each group consisted of 10 prepared roots, sixty samples were irrigated with NaOCl solution at different concentrations and times. Sixty samples were irradiated at different powers at the total irradiation time 30 and 60 sec, the remainder twenty samples were control positive and negative groups. Results: All concentrations, powers and times for both diode laser 1064nm and NaOCl had significant antimicrobial effects against E. faecalis. 5.25% NaOCl at both 2 minutes and 5 minutes had the highest antimicrobial effect with no significant differences from 3W laser/10sec and 2.5W/10sec for (6 cycles) but significantly different from 3W/5sec (6 cycles). The lowest effect was 0.5% NaOCl/2min that significantly different from all other groups. Conclusion: Diode laser device seems to be highly suitable for killing E. faecalis as NaOCl irrigant in root canals if appropriate energy and irradiated time used


Article
The effect of various endodontic irrigants on the sealing ability of Biodentine and other root perforation repair materials (In vitro study)

Authors: Ahmed K. Al-Zubaidi احمد الزبيدي --- Abdul Karim J. Al-Azzawi عبد الكريم جاسم العزاوي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 3 Pages: 1-8
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: This in vitro study was carried out to evaluate the effect of various endodontic irrigants (sodiumhypochlorite, ethylene diaminetetracetic acid and normal saline) on sealing ability of (Biodentine, mineral trioxideaggregate, and amalgam) used to repair furcal perforations.Material and methods: One hundred and twenty extracted human molars with divergent roots were used in thisstudy. A standard root canal access cavity was prepared in each tooth and furcal perforation was made and wasstandardized by using k file size 100 instrument to get a perforation of (1.32mm) in diameter .The teeth were randomlydivided in to three groups of 40 teeth according to the type of material used to repair the perforations (Group A: Thefurcal perforations were repaired with Biodentine, Group B: The furcal perforations were repaired with MTA ,GroupC: The furcal perforations were repaired with Amalgam). Each group was then subdivided into 4 subgroupsaccording to irrigation regimens applied over the repair site (Subgroup 1: without irrigation, Subgroup 2: the pulpchamber was gently irrigated with 10 mL 5.25% Sodium hypochlorite for 10 minutes, Subgroup 3: Pulp chamber wasgently irrigated with 10 mL 17% Ethylene Diaminetetracetic acid for 10 minutes, Subgroup 4: pulp chamber wasgently irrigated with 10 mL normal saline for 10 minutes. Each tooth was coated with two layers of nail varnish andthen sticky wax except 1 to 2 mm around the perforation site. Each tooth was placed in glass vial containing 3 ml ofbuffered Methylene blue dye at (37°C, pH 7) and kept in an incubator for 72 hour at 100% humidity. After dyeapplication, the teeth were washed in running water for 5 min. Each tooth was sectioned longitudinally in abuccolingual direction.Results: The results showed that group A has least mean of dye penetration and the difference was highly significantwith group C and non-significant with group B.Saline and NaOCl increase the sealing of all groups while EDTAsignificantly increased the dye penetration of Biodentine and MTA respectively.Conclusions: Biodentine has the best sealing ability of the tested materials while amalgam showed the highest dyepenetration of all tested materials. Saline and NaOCl increase the sealing ability of Biodentine and MTA where asEDTA decreased the sealing efficacy of MTA and Biodentine

الھدف من ھذه الدراسة كان لتقییم تأثیر مواد مختلفة لغسل قنوات الجذور (محلول الاثیلین دایمین تیترا استك اسد , محلول الھایبوكلوراید و المحلول الملحي العادي ) على قدرة الختملمواد (البایودنتین , مادة تجمع المعدن ثلاثي الاوكسد و الاملكم ) المستعملة في ترمیم ثقب مفترق الجذور .استخدمت في ھذه الدراسة مئة و عشرون ضرس سفلیة حدیثة القلع , متباعدة الجذور .تم تحضیر قناه الجذر القیاسیة لكل سن ثم عمل انثقاب لمفترق الجذر و تم معایرتة بحیث یصبحمقیاس 100 , قسمت الاضراس عشوائیا الى ثلات مجموعات تحوي على اربعین رحى استنادا لنوع المادة المستعملة في ترمیم انثقاب k بقطر ( 1,32 ملم ) بواسطة استخدام مبردمفترق الجذر كالاتي :مجموعة أ : رممت انثقابات الجذور بمادة البایودنتین .مجموعة ب : رممت انثقابات الجذور بمادة تجمع المعدن ثلاثي الاوكسد .مجموعة ج : رممت انثقابات الجذور بمادة الاملغمثم تم تقسیم كل مجموعة إلى 4 مجموعات فرعیة وفقا لنظم الري التي تم تطبیقھا على موقع إصلاح مفترق الجذور على النحو التالي:المجموعة الفرعیة الاولى : لم یجري أي ري .المجموعة الفرعیة الثانیة : تم ري غرفة اللب بلطف مع 10 مل من محلول الھایبوكلوراید 5.25 ٪ لمدة 10 دقائق .المجموعة الفرعیة الثالثة :تم ري غرفة اللب ب 10 مل من محلول الاثیلین دایمین تیترا استك اسد لمدة 10 دقائقالمجموعة الفرعیة الرابعة : تم ري غرفة اللب ب 10 مل من محلول الملحي المتعادل لمدة 10 دقائق2 ملم - تركت جمیع الأسنان لتجف لمدة 24 ساعة ثم ملئت الاسنان بالحشوة المؤقتة. كل سن تم تغطیتھ بواسطة طبقتین من مادة طلاء الاظافر تلیھا طبقة من الشمع اللاصق باستثناء 1حول موقع انثقاب مفترق الجذور .وضع كل سن في قارورة زجاجیة تحتوي على 3 مل من صبغة المیثیلین الزرقاء مخزنة في ( 37 درجة مئویة، ودرجة الحموضة 7 ) و حفظ في حاضنة لمدة 72 ساعة وبعد تطبیقالصبغة، تم غسل الأسنان في المیاه الجاریة لمدة 5 دقائق . تم قطع كل رحى طولیا على طول المحور الطولي في الاتجاه الدھلیزي اللساني من خلال الانثقاب باستخدام القرصالماسي . اظھرت النتائج ان المجموعة الاولى المرممة بمادة البایودنتین كان لھا القیمة الوسطیة النسبیة الاوطا لاختراق الصبغة و كان الاختلاف ذو فرق معنوي احصائي عال جدا معالمجموعة الثالثة المرممة بواسطة مادة الاملكم و كان الفرق بین المجموعة الاولى و الثانیة المرممة بمادة تجمع المعدن ثلاثي الاوكسد غیر معنوي .كما اظھرت النتائج ان المحلول الملحي العادي و و محلول الھایبوكلورایت یزید قابلیة الختم جمیع المجموعات في حین محلول محلول الاثیلین دایمین تیترا استل اسد سبب في زیادةكبیرة في اختراق الصبغة من البایودنتین و مادة و تجمع المعدن ثلاثي الاوكسد على التوالي.

Keywords

Biodentine --- MTA --- EDTA --- NaOCl --- root perforations


Article
An Evaluation of Antimicrobial Efficacy of Steralium, co+steralium, and 5% Sodium Hypochlorite against Enterococcus Faecalis Biofilm Formed on Tooth Substrate: (An in Vitro Study)

Author: Ayad M. Al-Kadhi
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2016 Volume: 28 Issue: 1 Pages: 11-16
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Enterococcus faecalis is emerging as an important endodontic pathogen, which can persist in theenvironment for extended periods after treatment and may cause endodontic failure. It is known to producebiofilms, a community of bacteria enclosed within a protective polymeric matrix. This study aimed to establishwhether the biofilm formation by Enterococcus faecalis can be inhibited with steralium, co+steralium, and 5%sodium hypochlorite in the root surface environment.Materials and Methods: Extracted human teeth were biomechanically prepared, vertically sectioned, placed in thetissue culture wells exposing the root canal surface to E. faecalis to form a biofilm. At the end of the 3rd and 6thweeks, all groups were treated for 10 minutes with the previously mentioned tested solutions and control and wereanalyzed qualitatively and quantitatively.Results: Qualitative assay with 3-weeks biofilm showed a complete inhibition of bacterial growth with co+steraliumand NaOCl, except steralium and saline, which showed presence of bacterial growth. In quantitative analysis,steralium and saline- treated tooth samples have 57.74 ± 5.39* CFU/ml., 140.71 ± 8.49 CFU/ml. respectively.Qualitative assay with 6-week biofilm showed growth when treated with steralium and saline treated tooth sampleshave 346.0 ± 78.88* CFU/ml., 127.18 ± 17.84 CFU/ml. respectively whereas co+steralium and NaOCl has showncomplete inhibition.Conclusions: co+steralium and 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalisbiofilm formed on tooth substrate. co+steralium showed statistically significant antibacterial activity. The use ofco+steralium as a root canal irrigant might prove to be advantageous considering the several undesirablecharacteristics of NaOCl


Article
Novel Approach of Oxidative Coupling Reaction with 1-Naphthol of the Simultaneous Metformin Drug Determination in Either Pharmaceutics Formulations or Environmental Samples of Water Using Homemade CFIA- Merging Zones Techniques

Author: Bushra Basheer Qassim
Journal: Ibn Al-Haitham Journal For Pure And Applied Science مجلة ابن الهيثم للعلوم الصرفة والتطبيقية ISSN: P16094042/ E25213407 Year: 2017 Volume: 30 Issue: 2 Pages: 54-68
Publisher: Baghdad University جامعة بغداد

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Abstract

A newly developed FIA-merging zones spectrophotometric system, the method is rapid, accurate and sensitive for metformin hydrochloride determination through the oxidation of 1- naphthol by sodium hypochlorite and coupling with metformin.HCl in the presence of sodium hydroxide to form a blue soluble ion pair and this product was determined using homemade CFIA-Merging zones techniques , at 580 nm. Data treatment shows that linear range is (0.5-35) μg/ ml. The optimization conditions for various chemical and physical conditions of [MTF- NaOCl-α-naphthol-NaOH] system were investigated. The LOD was 0.01μg / ml and LOQ 0.1μg/ml from the lowest concentration of the calibration graph with r2% 99.18 and RSD% did not exceed 3%, sample through put 48 sample/h while percentage recovery (Rec.%) were from 97-100% , indicate no interferences of the tablet excipients. The method was applied successfully, for estimation of metformin in environmental samples of water and pharmaceutical drugs. Statistical analysis of results was compared, shows the method can be regarded as an alternative analytical way for determining of metformin. hydrochloride in bulk and dosage forms samples


Article
The antibacterial effect of 10% Salvadorapersica (Siwak), 10% green tea, and 50% lemon juice on Enterococcus faecalis: an invitro study
التأثير المضاد للجراثيم لسلفادوريسا بنسبة 10٪ و سواك بنسبة 10٪ و شاي أخضر، و 50٪ من عصير الليمون على Enterococcus faecalis: دراسة خارجية

Authors: Venus Dilshad Najeeb --- Nsar Muhyaddin Aziz
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2018 Volume: 22 Issue: 3 Pages: 367-371
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Background and objective: Shaping and disinfection of the root canal with a combination of chemical agents and endodontic instruments play an important role in the success of endodontic therapy. This study aimed to evaluate the antibacterial properties of herbal alternatives as possible irrigates against Enterococcus faecalis during endodontic treatment compared with the conventional irrigation solutions such as NaOCl 5%.Methods: A 10% Siwak extract, 10% green tea extract and 50% fresh lemon juice were prepared separately to make antibiotic disks and seeded on the culture media respectively and compared with 5% NaOCl, distilled water as the control. Plates were left to incubate for 48 hours at 37Cₒ. Zones of inhibition of the bacterial growth were calculated to measure the antibacterial effect of the tested irrigants.Results: All irrigants were active against this bacterium. The 5% NaOCl had the highest mean value (27.70±0.66), followed by 10% Siwak (Salvadorapersica) (21.60±0.7), 50% lemon juice (15.10±0.56), and 10% green tea (10.50±0.5).Conclusion: Herbal extracts can be used as the possible irrigant to compensate 5% NaOCl during endodontic treatment.


Article
Effect of NaOCl and EDTA as Post Space Cleansing Solutions on the Bond Strength of Resin Cements to Root Canal Dentin (An in vitro Study)

Author: Raghad A Al-Askary
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2013 Volume: 13 Issue: 2 Pages: 266-274
Publisher: Mosul University جامعة الموصل

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Aims: To evaluate the regional bond strength of four resin-based luting cements to post space dentin after irrigating with different irrigant solutions regimens. Materials and Methods: Eighty extracted human lower premolars were decoronated, instrumented, and obturated. Post space was prepared in each root, then randomly divided into four groups (n=20) according to the final irrigation regimen for the post space, Gp.1: distilled water (DW) control; Gp.2: 5.25% NaOCl+DW; Gp.3: 17%EDTA+DW; Gp.4: 5.25%NaOCl+17% EDTA+DW. Each group was further subdivided into four subgroups (n=5) according to the luting cements, Gp.(1,2,3,4)A (Vivaglass®Liner); Gp.(1,2,3,4)B (PermaCem®-Dual); Gp.(1,2,3,4)C (Variolink II); Gp.(1,2,3,4) D (LuxaCore®Z-Dual), then each post-space was sectioned horizontally into three slices. A push-out test was performed to measure regional bond strengths and the fracture modes was evaluated using a stereomicroscope. Results: The use of 5.25% NaOCl+17% EDTA+DW result in higher bond strength than other treatments. While the control group gives the lowest. (LuxaCore®Z-Dual) showed the highest bond strength than other resin cements. (Vivaglass®Liner) gives the lowest bond strength. The regional bond strengths decreased significantly toward the apical third. The failure mode recorded was mostly adhesive in nature except for groups (3D,4D) showed some mixed failure at the coronal third. Conclusions: NaOCl+ EDTA flush had a profound effect on resin bond strength to radicular dentin. (LuxaCore®Z-Dual) composite core buildup material exhibited the higher regional bond strength to radicular dentin. The regional bond strength decreased from coronal to apical direction.

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