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Article
The Beneficial Role of Some Bone Markers in Evaluating Women with Osteoporosis under Different Therapeutic Regimens

Authors: Shatha H. Ali شذى حسين علي --- Shaima A. Abbass
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2011 Volume: 20 Issue: 1 Pages: 1-7
Publisher: Baghdad University جامعة بغداد

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Abstract

Osteoporosis is a systemic disease of the skeleton, characterized by low bone mass and alteration in the micro-architecture of the bone tissue that lead to an increase in brittleness with the ensuing predisposition to bone fracture. Global statistics shows that women are more exposed to this disease than men and in particular at menopause. This study was designed to evaluate the use of some bone markers: serum osteocalcin (Ost), alkaline phosphatase (ALP), as bone formation markers, also parathyroid hormone (PTH), calcium and inorganic phosphate level, for the assessment of patients with osteoporosis and to evaluate their role in monitoring of several types of therapeutic interventions (such as bisphosphonates, hormonal replacement therapy, and Ca and vit.D) in postmenopausal women.This study comprised of 36 women (age 51.67±5.14 years) those diagnosed to have osteoporosis, to be allocated randomly into three groups according to the type of therapy to be given as;
group A: received bisphosphonates (sodium alendronate 10mg/day) for twelve weeks (N=12).group B: treated with hormonal replacement therapy (conjugated estrogen 0.625mg/day) for twelve weeks (N=12). group C: received Ca and vit. D (Ca1500mg/day and cholecalciferol 1000IU/day) for twelve weeks(N=12). In addition to 15 perimenopausal healthy women to serve as a control group (age 51.13±7.62 years).The studied parameters were measured in serum obtained before starting treatment and after 12 weeks of therapy. Result indicated that the baseline values of both serum Ost and ALP were significantly higher in postmenopausal patients as compared to controls and serum Ost showed a significant reduction after treatment with alendronate compared to those treated with either HRT or Ca and vit. D.From this study we recommend estimating the baseline bone markers (Ost and ALP) status for newly diagnosed osteoporotic patients to be used as a guide for deciding the initial therapeutic intervention, and detection of non responder instead of waiting until patients develop further fracture while they are on therapy.

يعتبر مرض هشاشة العظام "ترقق العظام" من الأمراض التي تصيب الهيكل العظمي وتسبب خللا في النسيج العظمي ممايؤدي لنقص في كثافة العظام وتغيرات دقيقة في النسيج العظمي والتي تؤدي إلى زيادة هشاشة العظام وزيادة احتمالية التعرض لكسرالعظام. وتدل الإحصائيات العالمية إن معدل إصابة النساء بهذا المرض أكثر من الرجال خاصة بعد سن اليأس. يهدف البحث إلى تقيمفعالية استخدام بعض مؤشرات العظام في تشخيص المرض منها الاستيوكالسين ومحلل الفوسفات القاعدي كمؤشرات لبناء العظام,إضافة إلى قياس مستوى هرمون الباراثيرويد إلى جانب الكالسيوم والفوسفات اللاعضوية وفي متابعة المرض باستخدام علاجاتمختلفة مثل الفوسفونات الثنائية وعلاج الهرمونات التعويضي والكالسيوم وفيتامين دي. تضمنت الدراسة 36 من النساء اللاتي بعانين من± مرض هشاشة العظام وتم تقسيمهن إلى ثلاثة مجاميع وفقا للعلاج الذي أعطي لهن لمدة اثنا عشر أسبوعا .(بمعدل أعمار 51.675.14 سنة).المجموعة اأعطيتعقار الاندرونيت 10 ملغم/اليوم لمدة اثنا عشر أسبوعا وتضمنت 12 مريضة.المجموعة بأعطيتعلاج الهرمونات التعويضي من الاستروجين( 0.625 ملغم/اليوم)لمدة اثنا عشر أسبوعا وتضمنت 12 مريضة.المجموعة جأعطيتالكالسيوم مع فيتامين دي( 1500 ملغم و 1000 وحدة دولية يوميا) وتضمنت 12 مريضة.7.62 سنة ), تم قياس المؤشرات قيد الدراسة في ± إضافة الى خمسة عشر من النساء الاصحاء كمجموعة ضابطة بمعدل عمر( 51.13مصل المريضات قبل وبعد بدء العلاج بأثنا عشر أسبوعا .اظهرت النتائج بان المستويات البدائية للاوستياكالسين ومحلل الفوسفاتالقاعدي في المصل كانت مرتفعة في النساء في سن انقطاع الطمث والمصابات بترقق العظام مقارنة بالمجموعة الضابطة وانخفضمستوى الاوستياكالسين في مصل المريضات بعد علاجهن بالاندرونيت . من نتائج الدراسة يمكننا التوصية بتقييم مستوى مؤشراتالعظام للمريضات حديثات التشخيص بترقق العظام لتكون دليلا على اختيار العلاج المناسب وكذلك للكشف عن عدم الاستجابة بدلا منالانتظار لحدوث الكسور خلال استمرارهم على العلاج.


Article
Estimation of Serum Osteocalcin Levels in Osteoporotic Postmenopausal Iraqi Women with Type 2 Diabetes Mellitus
تقدير مستوى الاوستيوكالسين في مصول النساء العراقيات المصابات بهشاشة العظام والسكري النوع الثاني بعد سن الياس

Authors: Sabreen A. Muzal صابرين علي مزعل --- Kismat M.Turaki قسمة محمد تركي --- Kadhim K. Ghudhaib كاظم خضيرغضيب
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 4 Pages: 1549-1555
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Osteoporosis (OP) is a systemic disease characterized by low bone mass and micro architectural deterioration of bone tissue, resulting in an increased risk of fractures and has touched rampant proportions. Osteocalcin, one of the osteoblast-specific proteins, showed that its functions as a hormone improves glucose metabolism and reduces fat mass ratio. This study is aimed to estimate the osteocalcin and glucose level in blood serum of osteoporotic postmenopausal Women with and without Type 2 Diabetes.Materials and methods: 60 postmenopausal women with osteoporosis divided into two groups depending on with or without T2DM, 30 patients for each. Serum samples of 30 healthy postmenopausal women were collected as control group. Osteocalcin was measured by ELISA method using a kit of (CUSABIO. China). Glucose was determined by spectrophotometric method. Results: Mean serum osteocalcin in postmenopausal osteoporotic women without Type II Diabetes is higher than control group and the group with T2DM (p ≤ 0.001). Conclusion: Bone formation marker increases at postmenopausal osteoporosis women; Hyperglycemia also induces osteoblast function and reduces of production osteocalcin at T2DM.

المعلومات الاساسية: هشاشة العظام مرض يصيب الجهاز العظمي الذي يتميز بانخفاض كتلة العظم واعتلال التركيب الهندسي الدقيق لنسيج العظم، الذي يمكن ان ينتج في زيادة خطر نسب استتشراء الاصابة بالتكسرات للعظام. وجد ان الاستيوكالسين الذي يعد احد الهرمونات المتخصصة للخلايا البانية للعظم ، يعزز من ايض الكلوكوز ويقلل من نسبة كتلة الدهن. استهدفت هذه الدراسة تحديد مستوى الاوستيوكالسين والكلوكوز في مصول دم النساء بعد سن الياس المصابات بهشاشة العظم ومرض السكري او المصابات بالهشاشة فقط. المواد وطرائق العمل: قسمت 60 مريضة بهشاشة العظم الى مجموعتين على اساس مجموعة مصابة بالهشاشة والسكري نوع II ومجموعة مصابة بالهشاشة فقط دون السكري وبواقع 30 مريضة لكل مجموعة. تم جمع نماذج من مصول الدم لـ 30 مراة صحية كمجموعة ضابطة. تم قياس الاستيوكالسين بواسطة الايلايزا وباستعمال العدة التشخيصية (CUSABIO. China) فيما استخدمت الطريقة الطيفية في تحديد الكلوكوز. النتائج: لقد وجد ان معدل الاوستيوكالسين في مصل دم مجموعة النساء المصابات بهشاشة العظم بدون السكري يكون اعلى بالمقارنة مع مجموعة المصابات بهشاشة العظم والسكري معا والمجموعة الضابطة بمستوى p ≤ 0.001) ).الاستنتاج: ازدياد دالة تكوين العظم عند النساء المصابات بالهشاشة ، كذلك يحث فرط السكر من وظيفة الخلايا البانية للعظم ويختزل من انتاج الاوستيوكالسين في حالة الاصابة بمرض السكري.

Keywords

Osteocalcin --- Glucose --- Osteoporosis --- T2DM.


Article
Effects of light smoking on salivary levels of alkaline phosphatase and osteocalcin in chronic periodontitis patients

Authors: Lubaba A. Abdul Ameer لبابة عبد الامير --- Basima Gh. Ali باسمة غفوري علي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 2 Pages: 110-114
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic periodontitis is an inflammatory disease that affects the supporting tissues of the teeth and it’scommon among adults. Smoking is an important risk factor for periodontitis induces alveolar bone loss. Alkalinephosphatase enzyme is involved in the destruction of the human periodontium. It is produced by many cells such aspolymorphonuclear leukocytes, osteoblasts, macrophages and fibroblasts within the area of the periodontium andgingival crevice. Osteocalcin is one of the most abundant matrix proteins found in bones and the only matrix proteinsynthesized exclusively there. Smaller Osteocalcin fragments are found in areas of bone remodeling and are actuallydegradation products of the bone matrix.The purpose of this study was to evaluatethe effect of smoking on thesalivary alkaline phosphatase and Osteocalcin in subjects with chronic periodontitis compared to control subjects.Materials and Methods: Five ml of unstimulated whole saliva samples and full-mouth clinical periodontal recordings(plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level) wereobtained from study groups (25 light smokers and 33 non-smokerssubjects, both with chronic periodontitis) andcontrol groups (8 light smokers and 13 non-smokers subjects, both with healthy periodontium). All subjects weresystemically healthy males, with age range (30-50) years. Salivary Alkaline phosphatase and Osteocalcin levels weredetermined by Colorimetric and Enzyme-linked Immunosorbent Assays, respectively.Results: Smoker chronic periodontitis patients revealed non-significant differences in clinical periodontal parameterswith non-smoker counterparts (P o.o5) in terms of Plaque index, Probing pocket depth and Clinical attachment loss,with slight increase in plaque index value in smoker chronic periodontitis group(1.42±0.46) than non-smoker chronicperiodontitis group, while there were highly significant differences in terms of Gingival index and Bleeding onprobing(P ≤ 0.01).Osteocalcin levels were lower in smoker chronic periodontitis group (0.13±0.20) than non-smokerchronic periodontitis group (1.09±2.26) with significant difference (0.05 ≥ P > 0.01). Mean of Alkaline phosphataselevel was lower in smoker chronic periodontitis (11.14±4.53) than non-smoker chronic periodontitis (11.45±4.17) with anon-significant difference, while there was a significant difference inAlkaline phosphatase concentrations betweensmoker and non-smoker control groups.There were non-significant differences between smoker chronic periodontitisand smoker control groups in terms of Osteocalcin and Alkaline phosphatase concentrations. There were nonsignificantdifferences between non-smoker chronic periodontitis and non-smoker control groups in terms ofOsteocalcin and Alkaline phosphatase concentrations.Conclusion: Within the limits of this study, it may be suggested that suppression of salivary Osteocalcin levels bysmoking and slight increase in alkaline phosphatase in smokers groups, may explain the deleterious effects ofsmoking on periodontal health status.


Article
Periodontal Health Status and Assessment of Osteocalcin levels in Saliva of Diabetic Patients and Systemically Healthy Persons (Comparative study)

Authors: Zina Ali Daily --- Ayser Najah Mohammed
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2017 Volume: 29 Issue: 1 Pages: 89-95
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Diabetes and periodontitis are complicated prolonged disorders through a recognized two-way association. There is elongated-conventional mark that hyperglycaemia in diabetes is affected on immune-inflammatory response and disturb the action of osteoclast and in balance bone turnover, which might rise the person vulnerability to the progress of prolonged periodontitis. Osteocalcin is one of the greatest plentiful matrix proteins originate in bones and produced absolutely there. Small osteocalcin crumbles are noticed in regions of bone remodeling and are in fact degradation products of the bone matrix, that is released outside cells into the Gingival Crevicular Fluid (GCF) and saliva after destruction of periodontal tissue during periodontitisMaterials and Methods: Eighty patients with Type2Diabetes Maleates (T2DM), males and females, were recruited for the study, with an age range of (30-50) years were divided into four groups, (20 subjects each): poorly controlled Type 2Diabetes Mellitus with chronic periodontitis group (CP+pT2DM ) and well controlled Type 2Diabetes Mellitus with chronic periodontitis group(CP+wT2M) , group of patients with only chronic periodontitis (CP )and control group with healthy periodontium and systemically healthy. From all subjects five ml of unstimulated whole salivary samples were collected, then, the samples were centrifuged and the supernatants were collected and kept frozen until the biochemical analysis to measure OC concentrations then clinical periodontal parameters (plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment loss) were recorded for all subjects at four sites per tooth except for the third molars.Results: The results of this study revealed highly significant differences among all study and control groups for all the clinical periodontal parameters (plaque index, probing pocket depth, clinical attachment loss) ,and OC concentrations. Additionally patients had chronic periodontitis with poorly controlled Type 2Diabetes Mellitus(CP+pT2DM )demonstrated the highest median values of all clinical periodontal parameters and highest increase in levels of salivary OC followed by CP+wT2M group then CP and Control groups. The current study demonstrates the correlation between OC concentrations with each one of the clinical parameters.It revealed highly significant strong positive correlations with PLI, GI and BOP score 1, while highly significant strong negative correlations with PPD. Also, non-significant weak positive correlation existed with CAL in CP+pT2DM group. Also, high significant strong positive correlation with PLI, GI, BOP and CAL; while, non-significant weak positive correlation with PPD in CP+wT2M group . High-significant strong positive correlation with BOP and CAL, as well as, high significant moderate positive correlation with PPD and significant weak positive correlation with PLI, while non-significant weak positive correlation with GI existed in CP group .Finally, high significant moderate positive correlation with PLI and GI existed in the Control group.Conclusion: Patients with poor glycemic control had more severe periodontal tissue break down with increase in levels of OC than well controlled type 2 diabetic patients and non-diabetic patients all of them with chronic periodontitis. So, this biochemical marker may be useful of periodontal tissue destruction and allowed practitioners for early diagnosis, prognosis and efficient management of periodontal diseases and type 2 diabetes mellitus


Article
Effect of Atorvastatin on Bone biochemical markers in dyslipidemic patients, Basra, Iraq
تأثير عقار الا تورفاستاتين على المؤشرات الحيوية للعظام في المرضى الذين يعانون من داء الشحام،في البصرة-العراق

Author: Falah H. Shari فلاح شاري
Journal: nursing national Iraqi specility المجلة العراقية الوطنية للعلوم التمريضية ISSN: 18122388 Year: 2016 Volume: 29 Issue: 1 Pages: 51-62
Publisher: Baghdad University جامعة بغداد

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Abstract

Objective: The aim of the study was to estimate the action atorvastatin(20mg/day) on bone biochemical markers dyslipidemic men.Methodology: This study was conducted between May 2015 and November 2015 in Al-Basrah General hospital in Basra, Iraq, to evaluate important role of atorvastatin (20mg/day)(Lipitor® Pfizer Pharma GmbH.Germany) on bone biochemical markers. Thirty men patients who had been admitted for a variety of medical problems included in the study. All the patients had previously been diagnosed with Dyslipidemia by specialist physician in internal medicine and all patients age below 55 years . the inclusion criteria for the experimental group was use of atorvastatin for at least three months; the study group was then compared to age-matched healthy men .bone biochemical markers were compared between groups. Results are expressed mean ± SD; statistically Student’s t test was used to compare variables.Results: The mean age of the statin group was 44.5 ± 5.9 and control group was 45.7 ± 4.4.After 3 months treatment with atorvastatin, as in table (1) and figures (1-8) Surprisely level of serum total cholesterol remain significantly higher than value of normal healthy group. LDL value significantly reduced by treatment but still significantly higher than values of normal group. HDL value were significantly higher than that of its initial values, but level of HDL ;after treatment; was not significantly different from that of normal group. Triglycerides was not significantly changed by treatment and its value still significantly higher than normal group. Vit D level was not significantly change by treatment and its value was significantly lower than normal group. Osteocalcin was elevated significantly by treatment; and this elevation was significantly as compared with its initial value and with that of normal group. Amino Terminal telopeptide procollagen type I (NTX-I) was not significantly change by treatment and its value still significantly higher than normal group and before treatment values. Also body mass index value was not significantly changed by treatment. Recommendations: Further studies are recommended to confirm the positive effects of atorvastatin and other statins on bone biochemical markers with larger number of patients and longer period of follow up. Also other bone formation and resorption markers are recommended to determine the expected mechanisms of action of statins on bone remodeling processes.

الهدف : وكان الهدف من الدراسة لتقييم عمل الأتورفاستاتين (20 ملغ / يوم) على المؤشرات الحيوية لعظام الرجال المصابين بداء الشحام. المنهجية: وقد أجريت هذه الدراسة بين ايار 2015 وتشرين الاول من 2015 في مستشفى البصرة العام في البصرة، العراق، لتقييم الدور الهام للاتورفاستاتين 20ملغم من شركة فايزر الدوائية(لبتور,المانيا) على المؤشرات الحيوية للعظام. وقد شارك بالدراسة ثلاثين رجل من الذين ادخلوا للمستشفى نتيجة المشاكل الطبية المتنوعه. وقد تم تشخيص جميع المرضى المصابين بالشحام مسبقا من قبل الطبيب المختص في الطب الباطني. وجميع المرضى تقل أعمارهم عن 55 سنة من العمر. وكانت معايير الشمولية للمجموعة التجريبية للمرضى الذين استخدموا الأتورفاستاتين لمدة ثلاثة أشهر على الأقل. ثم قارن فريق الدراسة إلى تطابق سن الرجال الأصحاء . تمت مقارنة بين المؤشرات الحيوية بين المجاميع. تم التعبير احصائيا باستخدام المعدل ± الانحراف المعياري تم استخدام اختبار t للمقارنة بين المتغيرات. النتائج: وكان متوسط العمر لمجموعة الستاتين هو 44.5 ± 5.9 ومجموعة الاصحاء او السيطرة هو 45.7 ± 4.4 بعد ثلاثة اشهر من علاج الأتورفاستاتين، كما في الجدول (1) والاشكال من (1-8) تبين بصورة مفاجئة ان مستوى مجموع الكولسترول في الدم أعلى بكثير من مستوى مجموعة الاصحاء او السيطرة. كما تبين ان مستوى الدهون قليلة الكثافة انخفض انخفاضا كبيرا باستخدام العلاج ولكن لا يزال أعلى بكثير من قيم مجموعة الاصحاء. كما ان قيمة الدهون عالية الكثافه أعلى بكثير من القيم الأولية. ولكن مستوى هذه الدهون ، وبعد العلاج؛ كان لا تختلف كثيرا عن مجموعة الاصحاء الطبيعية. اما الدهون الثلاثية. لم تتغير بشكل كبير عن طريق العلاج وقيمته لا يزال أعلى من مجموعة الاصحاء. لم يتغير مستوى فيتامين دي بشكل كبير عن طريق العلاج وكان قيمتها قلت بشكل ملحوظ من مجموعة الاصحاء. بينما ارتفع مستوى الاوستيوكالسين بشكل ملحوظ عن طريق العلاج. وكان هذا الارتفاع بشكل ملحوظ بالمقارنة مع القيمة الأولية ومع أن مجموعة الاصحاء الطبيعية. مستوى التايلوبيبتيدات ذات الامينات الطرفية لم تتغير بشكل كبير عن طريق العلاج وقيمتها لا تزال اعلى من مجموعة الاصحاء والقيم الاولية (قبل بدء العلاج).التوصيات: اوصت هذه الدراسة بوجود الآثار الإيجابية للأتورفاستاتين (20 ملغ / يوم) على المؤشرات الحيوية للعظام على الاقل بعد ثلاثة أشهر من العلاج.وكذلك اوصت باستخدام عقار الاتورفستاتين لتقليل خطر الكسور بالنسبة لمرضى داء الشحام.


Article
In vivo immunohistochemical investegation of bone deposition at collagen coated Ti implant surface

Authors: Ban A. Al-Ghani بان الغاني --- Athraa Y.Al-Hijazi عذراء يحيى الحجازي --- Thair L.AL-Zubaydi Thair L.AL-Zubaydi
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2011 Volume: 23 Issue: special issue Pages: 47-52
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental implants provide a unique treatment modality for the replacement of lost dentition. Functionalsurface modifications by organic material such as collagen coating seem to enhance early peri-implant boneformation, enhancing the initial cell attachment. The aim of the study was to study the expression of osteocalcin andgrowth hormone receptor as bone formation markers in collagen coated and uncoated implant in intervalperiods(2,and 6 weeks).Materials and Methods: Commercially pure Titanium(cpTi) implants, coated with collagen protein, were placed inthe tibias of 32 New Zealand white rabbits, immunohistochemical tests for detection of expression of osteocalcin andgrowth hormone receptor were performed on all the implants of both control and experimental groups for (2, and 6weeks) healing intervals. Mechanical test (torque removal test) was performed as an indicator for the presence ofosseointegration and as a test for the mechanical property of bone-implant interface, because the torsion appearsto be primarily probing the interface mechanics.Results: Removal torque mean values in all studied groups (uncoated and coated with collagen at two and sixhealing intervals were increasing with advancing time (higher at 6 than 2 weeks periods). And coated implantshowed high value in comparison to control. Results have shown that positive reaction for OC&GHR was expressedby osteoblast cells (OB)at implants coated with collagen ,indicating that bone formation &maturation wasaccelerated by adding biological materials as a modification modality of implant surface..Conclusion: Immunohistochemical findings revealed high positive expression range from strong to moderate ofosteocalcin and GHR in coated implant in comparison to uncoated specialy in early periods.


Article
The relationship between levels of osteocalcin, calcium, vitamin K and vitamin D in sera of patients with bone fractures compared to apparently healthy control

Author: Yasir A. Al-Sudani1 , Essam F. Al-Jumaily1 , Ban A. AbdulMajeed2 , Munwar A. Al-Naqqash3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 42-46
Publisher: Baghdad University جامعة بغداد

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Abstract

The production of osteocalcin is stimulated by 1, 25 dihydroxy vitamin D and depends on vitamin K. Vitamin K increases the carboxylation of osteocalcin, but it does not increase its overall rate of synthesis. Although its function is not completely understood, osteocalcin may exist as a deposition site for hydroxyapatite crystals. It may also affect energy metabolism via the production and action of insulin. The present study aimed at measuring serum levels of osteocalcin, calcium, vitamin k and vitamin D in sera of patients complaining from different types of fractures compared to levels in apparently healthy controls. Serum osteocalcin was within normal limits in patients and controls. Serum calcium generally lower than normal, vitamin D was lower than normal and vitamin K was within normal limits. The results indicated the importance of measurement of vitamin D and serum calcium in patients with fracture.


Article
Partial Purification and Study of Osteocalcin from Blood of Human and Rats
تنقية جزئية ودراسة الاوستيوكالسين من دم الانسان والجرذان

Authors: Thikra A. Allwsh ذكرى علي علوش --- Liqa'a S. Abdulla لقاء سالم عبد الله
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2018 Volume: 27 Issue: 4E Pages: 19-28
Publisher: Mosul University جامعة الموصل

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Abstract

This study is concerned with attempts to isolate and purify osteocalcin from healthy human blood plasma and healthy rats blood serum using different biochemical techniques. This included precipitation by cold acetone and gel filtration chromatography. It was found that only the second peak (peak B) from human and rats had concentration of osteocalcin, and showed that fold of purification of isolated osteocalcin 57 and 43 for human and rats respectively.The results obtained from electrophoresis showed that one single band for isolated osteocalcin (peak B) from human and rats, and from high performance liquid chromatography showed that there was a good identical in retention time between the standard and isolated osteocalcin (peak B) from human and rats. The comparative molecular weight of partially purified osteocalcin from human plasma and rats serum was (6011.73 ± 400 Da) and (5248.07 ± 400 Da) respectively using gel filtration chromatography, and (5807.64 Da) and (4786.30 Da) respectively using electrophoresis technique.

تضمن البحث محاولة عزل وتنقية الأوستيوكالسين من بلازما دم شخص سليم ومصل دم جرذان سليمة باستعمال التقنيات الحياتية المختلفة، التي تضمنت الترسيب بالأسيتون البارد وكروماتوغرافيا الترشيح الهلامي، وقد وجد تركيز للأوستيوكالسين في الحزمة الثانية (الحزمة B) من الإنسان والجرذان ذو عدد مرات تنقية 57 و43 للهرمون المفصول من الانسان والجرذان على التوالي.بينت النتائج ظهور حزمة منفردة واحدة باستعمال الهجرة الكهربائية للأوستيوكالسين (الحزمة B) المعزولة من الإنسان والجرذان، وأن هناك تطابقاً في وقت الاحتباس باستعمال كروماتوغرافيا السائل عالي الأداء للأوستيوكالسين القياسي والأوستيوكالسين (الحزمة B) المعزولة من الإنسان والجرذان، وقد وجد ان الوزن الجزيئي التقريبي للأوستيوكالسين المعزول جزئياﹰ من بلازما دم الانسان ومصل دم الجرذان، هو (6011.73 ± 400 دالتون) و(5248.07 ± 400 دالتون) على التوالي باستعمال كروماتوغرافيا الترشيح الهلامي و(5807.64 دالتون) و(4786.30 دالتون) على التوالي باستعمال تقنية الهجرة الكهربائية.


Article
In vivo study of the effect of collagen protein coated implant as compared with implants coated with a mixture of partially stabilized zirconia and collagen on osseointegration

Authors: Thair L .AL-Zubaydi ثائر الزبيدي --- Athraa Y. Al-Hijazi عذراء يحيى الحجازي --- Ban A. Al-Ghani بان الغاني
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2011 Volume: 23 Issue: special issue Pages: 108-116
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Biocompatibility of orthopaedic surgical implants with bone tissue allows adequate osseointegrationbetween the bone and implant. To achieve this, implants are coated with biocompatible materials Commerciallypure titanium is widely used as dental implant material because of its suitable mechanical properties and excellentbiocompatibility,and to enhance osseointegration of the implant,organic and inorganic materials are used as abioinert coating material.Aim of the study: Comparison of the influence of the implant coated by biological material(collagen),and implantcoated by both bioinert ceramic(zirconia) and( collagen), on osseointegration by immunohistochemical , andradiographical studies with mechanical test.Materials and Methods: Commercially pure Titanium(cpTi) implants, coated with PSZ powder by electrophoreticdeposition (EPD) method, collagen protein, and mixture of collagen and partially stabilized zirconia(PSZ) wereplaced in the tibias of( 12 )New Zealand white rabbits, immunohistochemical tests for detection of expression ofosteocalcin and growth hormone receptor were performed on all the implants of both control and experimentalgroups for(3days,1, 2and6weeks) healing intervals.Mechanical test (torque removal test) was performed as anindicator for the presence of osseointegration and as a test for the mechanical property of bone-implant interface.Results: have illustrated that removal torque mean values, in all studied groups (uncoated and coated with both PSZand collagen at (2&6weeks) healing intervals were increasing with advancing time (higher at 6 than otherperiods),also results have shown that positive reaction for osteocalcin(OC)& growth hormone receptoe(GHR) wasexpressed by osteoblast cells(OB)at implants coated with collagen and implants coated with zirconia andcollagen,indicating that bone formation &maturation was accelerated by adding biological materials as amodification modality of implant surface..Conclusion: It is concluded that coating of implant with collagen and coating with both PSZand collagen showed anincrement in osseointegration in short interval period.


Article
Immunohistochemical detection to evaluate the biological role of Ti implants coated by a combination of fibronectin protein and hydroxyapitate (EPD) (in vivo study)

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Abstract

Background: physicochemical and biochemical coating techniques that are investigated now a day to enhancebone regeneration at the interface of titanium implant materials. The combination, however, of both organic andinorganic constituents is expected to result into truly bone-resembling coatings and as such to a new generation ofsurface-modified titanium implants with improved functionality and biological efficacy. This research was conductedto study the expression of osteocalcin and growth hormone receptor as bone formation markers in coated anduncoated implant in interval periods (3days,1,2and 6 weeks).,Materials and methods: Commercially pure titanium (CpTi) implants coated with hydroxyapatite by EPD method andwith fibronectin protein, were placed in the tibia of (16) New Zeland white rabbits , immunohistochemical tests fordetection of expression of osteocalcin and growth hormone receptor were performed on all the implants of bothcontrol and experimental groups (3days,1,2 and 6 weeks) healing intervals. Mechanical test (torque removal test)was performed as an indicator for the presence of osseointegration and as a test for the mechanical property ofbone-implant interface to be primarily propping the interface machanics.Results: The removal torque mean values in all studied groups uncoated and coated were increasing withadvancing time (higher at 6 than 2 weeks periods) and coated implant showed high value in comparsion to control.Result shows that Immunohistochemical findings revealed high positive expression range from strong to moderate forosteocalcin and growth hormone receptor in coated implant in comparison to uncoated. These results indicatingthat a mixing of bioactive HA ceramic and FN increased the activity of coated layer which improved the boneformation and maturation in bone-implant interface and enhance mechanical interlocking with bone.Conclusions: The present study concludes that organic and inorganic surface modification for titanium implantsurface by HA and FN enhances bone formation and increase osseointegration

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