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Article
Deficiency of Protein C and Protein S in Recurrent Pregnancy Loss

Authors: Hussein Naji Alshammary --- Hadi M.A. Almosawi --- Farah Salih Hadi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 2 Pages: 348-356
Publisher: Babylon University جامعة بابل

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Abstract

Recurrent pregnancy loss (RPL) is a common disorder that affects around 3-5% of pregnant women. It has different causes and in about 50% it is of unknown etiology. Normal pregnancy is associated with increased procoagulants, decreased fibrinolysis and decreased anticoagulants to maintain placental hemostasis during pregnancy. However, hypercoagulability or thrombophilia might be a risk factor with these changes especially in women with deficiency of any of natural anticoagulant factors. Protein C and protein S are natural anticoagulants and their deficiency was found to be associated with placental thrombosis, hypo perfusion, fetal death and fetal loss.The aim of the study is to identify protein C and protein S deficiency in women with recurrent pregnancy loss. 90 women were involved in the study, 45 of them have three or more miscarriages in the first and second trimester considered as patient group, 45 healthy women at time of full term delivery with at least one alive child as control group. Full history was taken from patients and controls. Blood investigations were done for protein C and protein S levels by using ELISA (enzyme linked immune sorbent assay).Platelets count was performed by auto analyzer (Ruby). The results showed a significant relation of low protein S with recurrent miscarriage (P =0.002) OR=2.250 (95%C.I. 1.764-2.870), while the relation of low protein C with recurrent miscarriage was not significant (P>0.05).There was a significant association of low protein S andlow protein C with the abortion occurred in the second trimester (P<0.05). Positive family history for thrombosis was significantly associated with recurrent abortion (p<0.05). Platelets count has non-significant association with recurrent miscarriage.There is a significant association of protein S deficiency with recurrent miscarriage especially in the miscarriages occurring in the second trimester.Thepositive family history of thrombosis is considereda risk factorfor recurrentmiscarriage.Protein C deficiency had no statistical significant.


Article
13- IMMUNOHISTOCHEMICAL ANALYSIS OF P53 PROTEIN IN COLORECTAL CARCINOMA AND ITS RELATIONSHIP TO CLINICOPATHOLOGIC FEATURES

Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2011 Volume: 17 Issue: 1 Pages: 85
Publisher: Basrah University جامعة البصرة

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Abstract

Hassanain H Khudier* & Daria Ismail Ameen@*MB,ChB, FIBMS Path., Department of Pathology, College of Medicine, University ofSulaimania. @MB,ChB, Pathology lab., gynecological and obstetric SulaimaniaTeaching Hospital.Correspondence to: Dr. Hassanain H. Khudair, Sulaimania teaching hospital, E-mail: hhk1970@gmail.comAbstractColorectal cancer regarded as one of the most widespread malignant tumor in the world.It is considered the second leading death factor among people in some developedcountries. Colorectal cancer comprises several distinct histological types includingadenocarcinoma which forms 85%-95% of all colorectal cancer cases. Pathogenesis ofcolorectal cancer is a multistep process characterized by involvement of many geneticalterations, including p53.The aim of this study is to detect the expression of p53 protein in colorectal carcinomaand to show its relationship with some clinicopathological features including age,gender, histological types, histological grades and staging.Forty paraffin-embedded tissue blocks of colectomy specimens were used in thisretrospective study. They were collected from the Department of Pathology in SulaimaniaTeaching Hospital, Shorsh Hospital and Shehid Saifeddin Private Clinic from January2007 to July 2008.Two sections of 4 micrometer thickness were taken from each paraffin embedded tissueblock. First section was taken for hematoxylin and eosin stain and the other one forimmunohistochemistry [anti-p53 monoclonal antibody] by using DakoCytomationEnvision + Dual Link System-HRP (DAB+). The relationship between p53 over expressionand the dependent variable (age, gender, histological types, histological grades, andstaging) were evaluated statistically using an analysis of variance (ANOVA) with STATA 8soft ware (College station, Tx). A positive reaction for p53 was scored on a semiquantitativebase as score 0 (no staining), score 1+ (weak staining), score 2+ (moderatestaining), and score 3+ (strong staining).Staining was negative for p53 (score 0) in 16 cases (40%). Positive cases were scored as(1+) in 4 cases (10.0%); (2+) in 8 cases (20.0%); and (3+), in 12 cases (30.0%). There wereno significant relationships between p53 over expression and age (p=0.682), gender(p=0.924), histological types (p=0.30), histological grades (p=0.516), and the stage of thedisease (p=0.281). Conclusions: Considering the p53 protein over expression in arelatively high percentage of patients, it seems that p53 mutation may play an importantrole in the development of colorectal carcinoma. There were no significant relationshipsbetween p53 protein expression and some clinicopathologic variables such as age,gender, histological types, histological grades, and the stage of the disease.


Article
INVESTIGATIONAL STUDY TO CHANGES OCCUR IN CONCENTRATION OF BLOOD SERUM PROTEIN DURING PREGNANCY IN IRAQI COW
دراسة استقصائية للتغيرات الحاصلة في تركيز بروتينات مصل الدم خلال فترة الحمل في الأبقار العراقية

Author: Rashad Fadhil Ghadhban. رشاد فاضل غضبان
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2008 Volume: 7 Issue: 1 Pages: 51-57
Publisher: Basrah University جامعة البصرة

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Abstract

A study was conducted on (35) healthy Frisian cow aged (2-4) years and were grown in farms in Al-zubair region, in Basrah city This study was performed to find the effect of pregnancy on blood serum protein concentration (Total protein, Albumin, Globulin, Globulin fractions (α, β, γ).The result revealed there was significant elevation (P<0.01) in concentration of Total protein, Albumin, Globulin, α, γ, Globulin between non pregnant and pregnant animal in 8th month. And there was no significant change occur on β, Globulin concentration.¬¬When compared the result between the 8th and 9th month of pregnant the result revealed very significant decrease (P<0.01) in the concentration of Globulin, α, γ, Globulin and significant decrease (P<0.05) in the concentration of Total protein, while significant increase in Albumin concentration, in the 9th month of pregnant, and there was no significant change in β, Globulin concentration.

أجريت الدراسة على 35 بقرة بعمر 2-4 سنة نوع فريزيان مضربة ، خالية من الأمراض سريريا ومتواجدة في عدد من مزارع قضاء الزبير- مدينة البصرة لمعرفة تأثير الحمل في تركيز بروتينات مصل الدم البروتين الكلي (Total protein )، الألبومين (Albumin)، الكلوبيولين (Globulin) وأجزاءه (ألفا وبيتا وكاما- كلوبيولين) .أظهرت النتائج ارتفاعا معنويا(P<0.01 ) في تركيز كل من البروتين الكلي والألبومين والكلوبيولين وأجزاءه (ألفا وبيتا وكاما- كلوبيولين) بين الحيوانات غير الحوامل و الحوامل إلى الشهر الثامن من الحمل، بينما تركيز البيتا كلوبيولين لم تظهر النتائج أي تغيير معنوي فيه.أظهرت نتائج مقارنة تركيز البروتين الكلي والألبومين والكلوبيولين وأجزاءه خلال الشهر الثامن والتاسع من الحمل وجود انخفاض معنوي(P<0.01) في تركيز الكلوبيولين وألفا والكاما كلوبيولين وانخفاض معنوي(P<0.05)في تركيز البروتين الكلي ،بينما كان ارتفاع معنوي(P<0.05) في تركيز الألبومين في الشهر التاسع مقارنة مع الشهر الثامن من الحمل ولم تظهر النتائج أي اختلاف في تركيز البيتا كلوبيولين

Keywords

: Globulin --- Serum --- Protein


Article
MEASURMENT OF SOME TRACE ELEMENTS AND PROTEIN OF HYDATID CYSTS FLUID IN SHEEP AND COW
قياس بعض العناصر النزره وكمية البروتين السائل لأكياس المائية في الأغنام والأبقار

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Abstract

The amount of four minerals (Magnesium, Calcium, Selenium and copper) and total protein of hydatid cysts fluid obtained from lung and liver of sheep and cow was checked. Magnesium (Mg+2) and calcium (Ca+2) showed higher levels in cysts of sheep than those of cow which was (8.38, 29.11, 7.90, 25.70 mmol/L) respectively whereas the selenium (Se+) and copper (Cu+) showed a little differences in both species. Total protein amonts were found to have wide range in both animal species.

الخلاصةشملت الدراسة قياس اربعة عناصر نزره وهي (المغنسيوم ، الكاليسوم، السيلينيوم والنحاس) على التوالي وكذلك كمية البروتين الكلية لسائل الأكياس المائية المأخوذ من رئات وأكباد الأغنام والأبقار ظهر آن نسبة المغنسيوم والكالسيوم في سائل الأكياس المائية للأغنام أعلى منه في الأبقار حيث بلغ (8.38 ،29.11 ، 7.90 ، 25.70) على التوالي بينما لم يظهر السيلينيوم والنحاس اختلافاً كبيراً في كلا النوعين كذلك فقد ظهرت كمية البروتين الكلية مستويات عالية من الاختلاف حيث بلغت (137.20 ، 145,40 ، 185.80 ، 90.35) في رئات وأكباد كلا النوعين وعلى التوالي.

Keywords

Protein --- Hydatid Cyst --- Magnesium.


Article
Changing in Collagen Protein Due to Aging
التغيرات الحاصلة ببروتين الكولاجين عند التقدم بالعمر

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Abstract

This study involved the elucidation of aging effects on the some biochemical characters of collagen that has been isolated from rabbit's lungs extract. Twenty-one albino rabbits aged between 1month-3.5 years, classified into 7 groups with 3 rabbits in each.Our results showed biochemical changes in collagens content which increased in rabbit's lung extractfrom 0.012g to 0.079gwhile the percentage of total collagen dry weight/lung dry weight increased in the group 1.5 years 0.094 compared with 1 month group 0.062 then return to decrease in 3.5 years group 0.062 compared to the 1.5 years group.This study also revealed structural changes of purified collagen from rabbits lung extracts with aging,purified collagen showed an increase of carbohydrates content and a decrease in fluorescents absorbance intensity.After using fluorescent spectrophotometric technique,while IR-technique didn't give us any differences in theimportant groups of collagen with aging, but it gave us a finger print similar to that of collagen type I.

شملت الدراسة الحالية بيان تأثير تقدم العمر على بعض الصفات الكيموحيوية للكولاجين الذي تم عزله من مستخلص رئات الارانب، حيث تم استخدام واحد وعشرون ارنباً نوع Albino تراوحت اعمارهم بين شهر واحد- ثلاث سنوات ونصف، صنفت الى سبع مجاميع بمعدل ثلاث ارانب لكل مجموعة. اظهرت النتائج تغيرات بايوكيمائية في محتوى الكولاجين التي ازدادت في مستخلصات رئات الارانب حيث تراوحت بين 0.012- 0.079غم بينما اظهرت نسبة وزن الكولاجين الجاف الكلي / وزن الرئة الجاف زيادة في مجموعة الارانب بعمر سنة ونصف حيث كانت 0.094 مقارنة بها في مجموعة الارانب بعمر شهر واحد 0.062 ثم عادت الى الانخفاض في مجموعة الارانب بعمر ثلاث سنوات ونصف0.062 مقارنة بمجموعة الارانب بعمر سنة ونصف.كما بينت الدراسة ايضاً وجود تغيرات تركيبية للكولاجين الذي تم تنقيته من المستخلصات الرئوية بتقدم العمر, فقد ازدادت نسبة الكربوهيدرات المرتبطة مع الكولاجين وقلت شدة الامتصاصية الفلورنسية والتي تم قياسها باستخدام تقانة قياس الشدة الفلورنسية Fluorescence Spectrometry Technique بتقدم العمر في حين لم نلاحظ اختلافاً واضحاً في المجاميع الفعالة باستخدام تقانة IR Infra-red بتقدم العمر ولكن هذه التقنية اوضحت بان الكولاجين المستخلص من انسجة رئات الارانب ذا بصمة اصبع مماثلة لبصمة الكولاجين نوع1.


Article
EFFECTS OF USING FISH BIOSILAGE AS FISH MEAL REPLACER ON FEEDING, GROWTH AND GUT HISTOLOGY IN COMMON CARP Cyprinus carpio L. FINGERLINGS

Author: Basim M. Jasim Sajed S. Al-Noor Salah M. Najim
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 35-47
Publisher: Basrah University جامعة البصرة

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Abstract

This study was carried out to evaluate the effects of locally produced fish biosilage as fish meal alternative on feeding, growth efficiency and gut histology in common carp C. Carpio fingerlings. Biosilage was prepared by fermenting marine by-catch fish with date fruit residues, domestic vinegar and citric acid. The produced biosilage was incorporated in feeds to replace 0, 25, 50 or 75% of fish meal protein. Fish were fed for 14 weeks and feeding and growth parameters were close in the four feed groups so as fish survival rate during the experiment (88.9-93.3%). Histological examination of intestine and liver sections has showed improvements when fish fed on the four different feeds. Initial fish group showed signs of nutritional deficiency through limited size of intestinal villi and hepatocytes. However, the histological structure of gut was improved after fish were fed on the experimental feeds without significant differences between fish meal or fish biosilage feeds. The study concluded that fish silage could replace fish meal without adverse effects on feeding, growth efficiency and gut histology.

Keywords

Biosilage --- fingerlings --- protein.


Article
Evaluation the antioxidant effect of α-L- Fucose injection into rabbit periodontium

Authors: Chenar A. Mohammad --- Khlood A. Al-Safi خلود الصافي --- Bakhtiar M. Ahmed
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 119-124
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: α-L-Fucose is a methyl pentose sugar similar to L-galactose except for the loss of alcohol group oncarbon number 6. The objective of this study is to evaluate the biochemical and antioxidant effect of intracrevicularinjection of fucose into rabbits periodontium, throughout measuring the levels of total protein (TP), total fucose (TF),protein bound fucose PBF) , Malondialdehyde (MDA) , and vitamin C in sera of fucose injected rabbit groups.(Materials and Methods: The existing study was carried out on 55 male rabbits and were divided randomly into threegroups ; first group was injected with 50μl of 150mM fucose solution into gingival sulcus ; second group was injectedwith 50 μl of normal saline ; while the third group was not received any injection ( normal group) .Blood sampleswere collected from injected groups at time intervals of 1, 3 ,24 ,72, and 168 hours after injection, for measuring ofserum TP, TF, , PBF , MDA, and vitamin C and compared with normal group.Results: The results showed a significant increase in the mean concentration of TF and PBF reaching its maximumvalue 3hrs after injection, then it decline until reached its normal value 168 hours after injection, whereas serum totalprotein increased significantly only 3 hours after injection. Also serum MDA level did not change after injection, whileserum vitamin C increased immediately after fucose injection, even 72 hours after injection.Conclusion: Intracrevicular injection of α-L- Fucose has an observable effect on TF and PBF this may give anindication about its effect on periodontal tissue and has a role in the body defence against oxidative stress,throughout increasing the production of vitamin C.


Article
A Study of Serum Protein Electrophoresis in Patients with Multiple Myeloma

Author: Hind Shakir Ahmed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 4 Pages: 494-498
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: Multiple myeloma (MM) is caused by the malignant proliferation of plasma cells through the bone marrow. The clinical features are due to disordered immunoglobulin synthesis and/or secretion from the cell. The amount of protein in the vascular compartment depends on the balance between the rate of synthesis and catabolism or loss. Electrophoresis is a technique that separates compounds such as proteins according to their different electrical charge. In this way, five main groups of proteins namely albumin and the α1, α2, β, γ-globulins, may be distinguished after protein staining and may be visually compared with those in a normal control serum. OBJECTIVE: To evaluate total serum protein, serum albumin, serum globulin, and serum protein electrophoresis in Iraqi MM patients. PATIENTS AND METHODS: This study was conducted during the period from August 2012 until the end of November 2012. The patients were admitted to Medical City Hospital and all the measurements were performed in the Medical Teaching Laboratories in Baghdad. Total serum protein was measured by colorimetric Biuret method, while serum albumin was measured by bromocresol green method, serum globulin, and serum protein electrophoresis were measured in 25 patients with MM; their age range was (50-70) years and compared with 25 healthy controls.RESULTS: Total serum protein and serum globulin were significantly increased (86.76±17.05 g/l and 55.36±20.54 g/l respectively) in patients with MM, as compared with their controls, (P=0.0001). While a significant decreased was found in serum albumin (29.6±8.77 g/l) and albumin to globulin ratio (0.64±0.36 g/l) for patients with MM as compared with their controls, (P=0.0001). CONCLUSION:Abnormal concentrations of total serum protein, serum albumin, serum globulin, and M-band are associated with MM


Article
Study The Interaction between LH, FSH, and TSH with New Synthesized Magnetic Nanoparticles Coated with Dextran

Authors: Fatin Fadhel Al-Kazazz --- Hussain Kadhem Al-Hakeim --- Habiba Khdair Al-Aobaid
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 2 Pages: 421 -434
Publisher: Babylon University جامعة بابل

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Abstract

Nanoparticle (NP) was used in vivo and in vitro in contact with the body fluids that contain different types of proteins. The proteins have the ability to adsorb on NPs surface, and alter its activity due to the modification of the secondary and tertiary structures. In this study, the interaction between coated magnetic nanoparticles (MNP@Dextran) and the hormones (LH, FSH, and TSH) were studied in details. The possible change in the secondary and tertiary structure of these parameters is discussed. The (MNPs) were prepared using co-precipitation method of Fe3O4 followed by reacted these MNPs with Dextran under an optimum conditions to produce coated MNP@Dextran. Different concentrations of each hormone were incubated with a known amount of MNP@Dextran and the quantity of the hormones adsorbed was calculated. The experiments were repeated at different temperatures to calculate the thermodynamic parameters. Secondary structures of hormones were examined using circular dichroism (CD).Tertiary structures of protein were measured using fluoroscopy technique.The adsorption process obeyed Freundlich adsorption isotherm indicating heterogeneity of the MNP@Dextran surface,and it associated with the changing in the secondary and tertiary structure of hormone after adsorption on MNP@Dextran.


Article
PURIFICATION AND CHARACTERIZATION OF SALIVARY AMYLASE INHIBITOR EXTRACTED FROM BARLEY
تنقية وتوصيف مثبطات ألفا- اميليز لعاب الانسان من محصول الشعيرHordeum vulgare

Authors: S. C. Abood صبري جثير عبود --- I. M. Hakeem ابتهاج مصطفى حكيم
Journal: Iraqi Journal of Agricultural Science مجلة العلوم الزراعية العراقية ISSN: 00750530/24100862 Year: 2016 Volume: 47 Issue: 4 Pages: 1039-1049
Publisher: Baghdad University جامعة بغداد

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Abstract

Amylase inhibitors were purified by many sequential steps included concentration by gradual addition of ammonium sulfate at saturation ratios. ranged from 0 to 90% . The best ratio of saturation was found to be 70% as the specific activity and inhibition activity toward Human alpha-amylase(HAS) were the highest ( 8 U/mg and 6 U/ml respectively as compared to those of the rest ratios, the ratio of saturation with ammonium sulfate 60 % and then 50%, (5.8 ,5.5 )U/ml and( 7.7 ،7 )U/mg respectively for inhibition activity and specific activity and for 40% ,30%20% saturation the inhibition activity and specific activity were(5 ،4.8 ،4 ) u/ml (6.6 ،6 ،5.8) u/mg respectively .The precepitation step was followed by ionic exchange chromatography technique by DEAE-cellulose column( 3×11 )cm and the results showed that there was one peak with inhibition activity toward (HAS). Further purification steps were conducted using gel filtration on Sephacryl S-200 column (1.5 × 60)cm; the purification folds was5.59 times with outcome of 46.5%.The results of alpha-amylase inhibitors characterization showed that the molecular weight was about 23.44 and 22.9 kDa as determined by electrophoresis and gel filteration respectively.

نقي مثبط اميليز اللعاب من بذور الشعير بعدة خطوات متسلسلة اشتملت على التركيز بأستخدام كبريتات الامونيوم بنسب اشباع متدرجة تراوحت بين (0-90 )% اذ تبين زيادة الفعالية التثبيطية والفعالية النوعية لمستخلص الشعير بزيادة نسب الاشباع بكبريتات الامونيوم اذ نجد ان افضل نسبه اشباع بكبريتات الامونيوم كانت عند 70%. اذ اعطت اعلى فعالية تثبيطية وفعالية نوعية لمستخلص الشعير( 6 وحده/مل ، 8 وحده/ملغم بروتين على التوالي ) بالمقارنة مع نسب التشبع الاخرى 60% ثم 50% اذ بلغت الفعالية التثبيطية 5.8 و5.5 وحده/مل والفعالية النوعية (7.7 ،7) وحده/ملغم بروتين على التوالي اما نسب الاشباع 40% ، 30% ، 20% فقد اعطت قيماً اقل اذ بلغت الفعالية التثبيطية (5 ،4.8 ،4) وحده /مل والفعالية النوعية (6.6 ،6 ،5.8) وحده/ملغم بروتين على التوالي، وانخفضت ايضاً الفعالية التثبيطية والنوعية عند نسب الاشباع 80% ، 90% اذ بلغت (5 ،4.5) وحده/مل (6.2 ،5.1) وحده /ملغم بروتين على التوالي ، واعقب خطوة الترسيب استخدام تقنية كروماتوكرافيا التبادل الآيوني بعمود DEAE-cellulose بأبعاد ( 3×11 )سم تمت الموازنة بأستخدام محلول الترس الداريء بتركيز 0.005 مولار ورقم هيدروجيني 8 استردت الاجزاء المرتبطة بالمبادل بمحلول الموازنة نفسه والمحتوي على تركيز متدرجة من كلوريد الصوديوم واسفرت النتائج عن وجود قمة واحدة في مرحلة الاسترداد امتلكت فعالية في تثبيط فعالية انزيم اميليز اللعاب ، واجريت خطوات تنقية اضافية متمثلة بأستخدام تقنية الترشيح الهلامي على عمود السيفاكريل S-200 بأبعاد ( 1.5 × 60 ) سم فكان عدد مرات التنقية 5.59 وبحصيلة مقدارها 46.5 % ، اظهرت نتائج توصيف مثبط الألفا-اميليز المنقى من الشعير ان القمةاعطت حزمة واحدة عند ترحيلها بأستخدام تقنية الترحيل الكهربائي وقد بلغ الوزن الجزيئي حوالي 23.44 كيلودالتون لقمة المثبط من الشعير في حين بلغ الوزن الجزيئي لقمة المثبط بأستخدام تقنية الترشيح الهلامي على عمود السيفاكريل S-200 تقارب 22.9 كيلودالتون.

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