research centers


Search results: Found 3

Listing 1 - 3 of 3
Sort by

Article
Expression of RANKL by dental cells during eruption of mice teeth

Authors: Lubna K. Jassim لبنى جاسم --- Athraa Y. Al- Hijazi عذراء يحيى الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 1 Pages: 76-81
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background : In order for a tooth to erupt, two obvious requirements are needed. First, there has to be alveolar boneresorption of the bone overlying the crown of the tooth such that an eruption pathway is formed. Second, resorptionof bony crypt and apposition of new one, third, there has to be a biological process that will result in the toothmoving through this eruption pathway.The amniotic sac contains a considerable quantity of stem cells. Theseamniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for humanapplication. Receptor activator of nuclear factor kappa B ligand (RANKL) is concentrated on bone biology, morespecifically bone metabolism. RANKL plays a vital role in osteoclastogenesis for bone resorption. This study aimed toevaluate the expression of RANKL marker by dental cells during eruption of the teeth.Materials and Methods: : forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cellsin the anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 micefor each period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: The present results localized and identified RANKL marker in 3 areas of developing tooth of the studied groupsincludes overlying, surrounding and apical bone. Positive RANKL with high significant value expressed by osteoclastof overlying bone in Amnion group followed by Control at day 4. In surrounding bone positive expression of RANKLillustrated to be highest in Control followed by Amniotic fluid at day 10.Apical bone shows positive expression ofRANKL in amniotic fluid group and it records to be the highest value in comparison to studied groups at day 10.Conclusion Expression marker RANKL illustrates that amniotic fluid group has a high expression of RANKL in osteoclastsurrounding and apical bone areas while control expressed RANKL in osteoclast of overlying bone. The present resultsopened clinical hopes in dental tissue engineering by application of autologous amniotic fluid and chorion cells

Keywords

RANKL --- tooth eruption


Article
Salivary level of RANKL and OPG in chronic periodontitis

Authors: Batool Hassan Al-Ghurabi بتول حسن الغرابي --- Sara Mohammed Mohssen سارة محمد محسن
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 1 Pages: 189-194
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Periodontal diseases are initiated by microbial plaque, which accumulates in the sulcular region andinduces an inflammatory response. The Receptor activator of nuclear factor-kappa B ligand / osteoprotegerin(RANKL/OPG) axis is involved in the regulation of bone metabolism in periodontitis, in which an increase in receptoractivator of nuclear factor-kappa B ligand or a decrease in osteoprotegerin can tip the balance in favor ofosteoclastogenesis and the resorption of alveolar bone that is the hallmark of periodontitis. This study was performedto investigate the role of salivary levels of RANKL and OPG in pathogenesis of chronic periodontitis.Subjects and Methods: Fifty five subjects with chronic periodontitis with ages range from 24-64 years and 25apparently healthy volunteers their ages and sexes were matched with the patients were participated in this study.Periodontal parameters used in this study were plaque index, gingival index, probing pocket depth, clinicalattachment level and bleeding on probing. Saliva samples were collected from all patients and controls. Enzymelinkedimmunosorbent assay was carried out for estimation the salivary level of RANKL and OPG in studied groups.Results: The present data revealed that the median salivary levels of RANKL was elevated in patient as comparedwith control group (P<0.001), whereas the salivary levels of OPG doesn’t show any significant differences betweenthe study groups (p>0.05). In contrast the ratio of RANKL/OPG ratio was significantly higher in patients whencompared with the ratio in control group. Furthermore, negative significant correlation was noticed between RANKLand OPG. Regarding correlation between salivary (RANKL and OPG) and clinical periodontal parameters, RANKLlevels was showed significant positive correlation with each of probing pocket depth and clinical attachment level.Otherwise no association between OPG levels and clinical parameters of periodontitis was found. Moreover; theratio RANKL/OPG was showed significant positive correlation with each of gingival index, probing pocket depth andclinical attachment level.Conclusion: This study demonstrates that salivary levels of RANKL and OPG play a crucial role in pathogenesis ofperiodontitis, and the relative RANKL/ OPG ratio appears to be indicative of disease occurrence

Keywords

chronic periodontitis --- RANKL --- OPG


Article
A Comparative Study of Clinicopathological and Immunohistochemical Expression of CD1a, RANK and RANKL in Langerhans Cell Histiocytosis of Jaw and Skull Lesions

Authors: Alyaa Kadhim Mohammed علياء كاظم محمد --- Bashar Hamid Abdullah بشار حميدعبد الله
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2016 Volume: 28 Issue: 1 Pages: 78-83
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Langerhans' cell histiocytosis (LCH) is a group of conditions affecting the reticuloendothelial system. Itincludes Letterer-Siwe disease, Hand-Schuller-Christian disease and eosinophilic granuloma and most often presentsin childhood.Materials and methods: Twenty-five cases of LCH were diagnosed histologically and confirmed by CD1a antibodyand assessed immunohistochemically using anti-RANKL and anti-RANK antibodies to evaluate osteoclastogenicmechanism.Results: Regarding jaw cases, there was a significant correlation between CD1a and RANK (P=0.016). While in theskull, highly significant correlation existed between RANK and RANKL (p=0.001). Among the sites, there was nostatistically significant difference found for each the immunohistochemical markers used.Conclusion: LCH of jaws and skull bear similar osteoclastogenic mechanism when quantified with RANK and RANKLimmunostaining respectively. With a significant correlation between CD1a and RANK for jaw cases, while in the skulllesions, there was a high significant correlation between RANK and RANKL.

Listing 1 - 3 of 3
Sort by
Narrow your search

Resource type

article (3)


Language

English (3)


Year
From To Submit

2016 (1)

2015 (1)

2013 (1)