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Study of Phylogenetic Tree and Morphology of Aporrectodea Based on Mitochondrial Marker (16S rRNA gene) in Some Area South of Baghdad/ Iraq

Author: Najwa Sh. Ahmed1, Nebrass Faleh Chacain2, Falih Hamzah Edan3,Saad M. Nada1, Anas Noori Ibraheem1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2015 Volume: 14 Issue: 2 Pages: 47-54
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to show the phylogenetic structure of Aporrectodea genus in order to verify its cladistics nature and its taxonomic validity. In this work, collection of Aporrectodea genus from three locations from South of Baghdad, (AL-Karrada, AL-Zafranya and New Baghdad) are studied. First, we used usual morphological characteristics to identify each species than molecular phylogenetic analyses are based on the sequences of mitochondrial 16S rRNA gene regions and used software MEGA6 and Raptorx software. Rresults of the two methods (MEGA 6 and Raptorx software) were cluster groups (organisms of 8 sample from Group1A and Group3) in one group and with distance equal to 0.006, clustering of group 2 as a single group, and reached the highest value between group 2 and group 1(B) with distance equal to 0.272 and to move away genetic traits, Raptorx software, conformation of protein for 16SrRNA appeared as a result of the similarity of Mega6. The marker mitochondrial 16S rRNA gene is a powerful tool for identifying species of earthworms and provides a useful complement to traditional morphological taxonomy.


Article
Study of Signal Transduction Hypothetical Proteins of Helicobacter pylori
دراسة للبروتينات الافتراضية الناقلة للاشارات في البكتريا Helicobacter pylori

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Abstract

Abstract: Hypothetical proteins of Helicobacter pylori strain 26695 were screened using MBGD database. There were about 714 (41.58 %) hypothetical proteins, 82.2 % were virulent proteins. Hypothetical proteins were categorized according to Gene Ontology grouping using ProtFunc server. Proteins in signal transduction group were selected for studying their characters. Post-translation modifications (PTMs) such as phosphorylation gave unclear results, Glycosylation such as N-linked Glycosylation was more frequent than O-linked Glycosylation among proteins. Secondary and tertiary structures were determined and pbd homologous (assigned with X-ray and NMR techniques) were assigned. Most proteins showed high interaction with cellular proteins and components.

الخلاصة: اجري مسح للبروتينات الافتراضية للبكتريا Helicobacter pylori السلالة 26695 وكان عددها 714 (41.58 %) من بروتينات / جينات البكتريا المدرجة في قاعدة البيانات MBDG . مسحت البروتينات لتحديد ضراوتها وكان 82.2 % هي بروتينات ضراوة . استعمل ProtFunc Server لتحديد مجاميع البروتينات الوظيفية وفقا لتصنيف Gene Ontology ، اختيرت البروتينات العاملة في نقل الإشارات من البروتينات الافتراضية نظرا لأهميتها وهي (HP0085 ، HP0079، PS235، HP0203، HP0155 ، HP0249، HP0583، HP0856، ST515 ، HP1527، PS1632، HP1163 ، PS1350، HP1085 ، HP1436). درست إمكانية حدوث التحويرات بعد الترجمة مثل الفسفرة التي لم تظهر نتائج واضحة لعدة أسباب ، درس ايضا إضافة السكريات N-Glycosylation و O-Glycosylation وكانت الحالة الأولى أكثر تكرارا . تم تحديد التراكيب الثنائية والثلاثية للبروتينات وإيجاد البروتينات الأقرب التي لها تراكيب محددة بالأشعة السينية والرنين النووي المغناطيسي . أظهرت نتائج التداخل مع مكونات الخلية ان هناك تداخلا كبيرا لمعظم البروتينات المدروسة .

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