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Article
THE ROLE OF REACTIVE OXYGEN SPECIES IN THE CHEMILUMINESCENCE REACTION OF
دور الجذور الأوكسيجينية الحرة في استثارة التألق الكيميائي المحدث من أكسدة الهايبوكلورايد بواسطة محلول اللومينول

Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2005 Volume: 31 Issue: 1A Pages: 41-54
Publisher: Basrah University جامعة البصرة

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Abstract

The oxidation of luminol by hypochlorite to generate chemiluminescence (CL) has beenstudied by using a multipurpose photon counting system. The processes of light production ,which invovle the reactive oxygen species (ROS), are discussed in terms of the NaOCl-Luminolreaction .The addition of radical scavengers such as ascorbic acid and dimethylsulfoxide (DMSO) leadto decreases in the peak of CL reaction of both system NaOCl-Luminol and FeSO4 – Luminolrespectively, while the addition of persulfate lead to increase in the peak of CL. The resultssuggest that ROS radicals might be constant factors in CL reaction of NaOCl-Luminol system

تم دراسة التألق الكيميائي المحتث من أكسدة اللومينول بواسطة محلول الهايبوكلورايد باستخدامالعداد الفوتوني المتعدد الأغراض البحثية. حيث تم مناقشة العمليات الحادثة في تفاعل اللومينول-هايبوكلورايد والتي تشمل تفاعلات جذور الأوكسي الحرة.أحدثت إضافة قانصات الجذور الحرة مثل حامض الاسكوربيك ومحلول ثنائي مثيل سلفوكسايدنقصان في قمة التألق الكيميائي في كل من تفاعل اللومينول-هايبوكلورايد وتفاعل اللومينول-كبريتاتالحديدوز على التوالي بينما كان تأثير إضافة البرسلفات على تفاعل اللومينول-هايبوكلورايد زيادة فيالتألق الكيميائي. النتائج المقترحة هنا تشير على إن الجذور الحرة ربما تعتبر عوامل أساسية ثابتة فيتفسير تفاعل اللومينول-هايبوكلورايد


Article
BIOCHEMICAL STUDY ON SUPEROXIDE DISMUTASE ENZYME IN PATIENTS WITH DIFFERENT BRAIN TUMORS
العلاقة بين مستضدات التطابق النسيجي- الصنف الثاني والفعالية الوظيفية للخلايا اللمفية بعد تحفيزها بمستضدات الحمات المعوية والحمات الغدية في الاطفال المصابين حديثاً بالسكري من النوع الأول

Author: حذامة رزوقي حسن
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 2 Pages: 191-201
Publisher: Baghdad University جامعة بغداد

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Abstract

The aim of the current study is to in evaluate the role of SOD activity in the previously reported oxidative stress in our laboratory(1), in the patients with different brain tumors. SOD activity was assayed according to riboflavin/NBT method and its specific activity was calculated in patients with benign and malignant brain tumors and control. Moreover the specific activity was compared in these samples according to gender and the occurrence of disease.Non significant elevation (P > 0.05) in SOD specific activity was observed in tissue of malignant tumors in comparison to that of in benign brain tumors. While a highly significant decrease (P < 0.001) of the specific activity was found in sera of malignant patients group in comparison to that of the control group, and it was found lower in female than male in control and malignant groups. An elevation in this specific activity was noticed in patients with secondary brain tumors in comparison to that of primary brain tumors (P<0.05). From the results of the present study we conclude that the observed decrease in SOD activity in sera of patients with different type of brain tumors contribute to the oxidative stress that previously reported in our laboratory to be present in such patients.

الهدف من هذه الدراسة هو تحديد دور فعالية انزيم SOD في الجهد التأكسدي والذي سبق وان سجل وجوده في أنسجة ومصل الدم من قبل الباحثين في مختبرنا في المرضى المصابين بأورام الدماغ المختلفة. لقد تم تقدير فعالية انزيم SOD بإعتماد طريقة riboflavin/NBT والفعالية النوعية لهذا الانزيم في المرضى المصابين بأورام الدماغ الحميدة والخبيثة والأصحاء، فضلا عن ذلك فقد تمت مقارنة الفعالية النوعية في المجاميع المدروسة إعتماداً على الجنس.أظهرت النتائج ارتفاع غير ملموس في الفعالية النوعية لانزيم SOD في انسجة المصابين بأورام الدماغ الحميدة P>0.05 في حين لوحظ انخفاض ملموس جداً في هذه الفعالية النوعية في مصول المصابين بأورام الدماغ الخبيثة مقارنة مع الاشخاص الأصحاء P<0.001. كما وجد ان الفعالية النوعية لهذا الانزيم منخفضة في النساء مقارنة بالرجال لمجاميع الاصحاء والمصابين بأورم الدماغ الخبيثة، وعند مقارنة الفعالية النوعية لهذا الانزيم في مصول الأشخاص المصابين بأورام الدماغ الخبيثة الابتدائية مع تلك للمصابين باورام الدماغ الخبيثة الثانوية، أظهرت النتائج وجود زيادة فيها في مصول أولئك المصابين بأورام الدماغ الخبيثة الثانوية (سرطان الدماغ) مقارنة مع أولئك المصابين بالأورام الإبتدائية فيها. ممكن الاستنتاج من نتائج الدراسة الحالية ان الانخفاض الملحوظ في فعالية الانزيم SOD في مصول المرضى المصابين بأنواع مختلفة من أورام الدماغ يساهم في الجهد التأكسدي الذي سبق وان سجل عند هؤلاء المرضى في مختبرنا.


Article
Study of Some Markers of Oxidative Stress Statues in Cardiovascular Disease Patients

Authors: Yasmeen Muhialdeen H. --- Alaa H. Jawad --- Wisam Kadhum H. Al-Hashemi
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2015 Volume: 18 Issue: 3 Pages: 38-43
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The oxidative stress is one of the main cause for cardiovascular diseases and also one of the results of these diseases, its development. The aim of this study was to investigate the relationship between of some oxidative stress markers and cardiovascular diseases. This study involved 84 samples matched ages and sex divided into groups; 28 samples cardiovascular and 28 hypertensive in addition to 28 healthy control group. The following analysis were done: lipid profile, malondialdehyde (MDA), and albumin. The results show there is a significant increase in lipid profile between each group and control group. MDA was significantly higher in patients compared to controls whereas no significant difference was found in albumin between patient and control groups. The increase in MDA which is a product of lipid peroxidation is attributed to destruction in body cell caused by free radicals and resulting oxidative stress. It has been concluded that the assessing the lipid ratios is important even in a normal individual as it is one of the factors for development of cardiovascular diseases and their complications. There is a positive relation between oxidation results from these diseases and their developments.

الجهد التاكسدي هو احد الاسباب الرئيسية لامراض القلب واوعيته وهو ايضا احد نتائج تقدم هذه الامراض والتحطم الناتج منها. هدف هذه الدراسة هو بحث العلاقة بين مؤشرات الجهد التاكسدي وامراض القلب واوعيته. هذه الدراسة استلزمت 84 عينة من النساء قسمت الى 28 عينة مرضى بامراض القلب واوعيته و 28 بأمراض ضغط الدم بالإضافة الى 28 من الاصحاء كمجموعة سيطرة. التحليلات التالية اجريت لكل العينات: صورة الدهون، المالون داي الديهايد، والالبومين. النتائج اظهرت ان هناك زيادة معنوية في مستويات صورة الدهون بين كل مجموعة ومجموعة السيطرة. المالوندايالديهايد اعلى بشكل معنوي في المرضى مقارنة بمجموعة السيطرة بينما لم يظهر اي فرق معنوي في الالبومين. الزيادة في المالون داي الديهايد والذي هو ناتج فوق اكسدة الدهون يعزى الى التحطم في خلايا الجسم بسبب الجذور الحرة الناتجة من الجهد التاكسدي. الاستنتاج: اهمية تقييم نسب الدهون حتى في الاصحاء كونها احد عوامل تطور امراض القلب واوعيته وتعقيداته. هذه العلاقة الموجبة بين الاكسدة الناتجة من هذه الامراض وتفاقمها. وبالتالي يقترح زيادة تناول مضادات الاكسدة للحد من هذه الامراض او تفاقمها.


Article
Evaluation of antioxidants and reactive oxygen species (ROS) levels after combination exposure to chromium (III) and atrazine on liver in Wister Albion male rats

Author: Sabah H. Enayah
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 67-77
Publisher: Baghdad University جامعة بغداد

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Abstract

Atrazine (ATZ)is remaining as one of the most widely broad spectrum pesticide used to control on annual grasses and broadleaf weeds. While Chromium Cr (III) it has been used currently as food supplemented. The current study was designed to determine impact of Cr at 7,30 and 300ppm on liver was in absence or in the present of (25 and 50mg/kg) of ATZ for 6 weeks in rats. Results indicated that deficiency or supplementation with Cr did not cause measurable toxicity and has no obvious effect on oxidative stress or anti-oxidant enzymes and lipid metabolism. Furthermore, the results showed that the mixture increased level of Aryl Hydrocarbon Receptor (AhR), Reactive Oxygen Species (ROS). Moreover, the mixture had effects on antioxidants such as super oxide dismutase (SOD), Catalase (CAT), Reduced glutathione (GSH), activity where demonstrated more sever in liver tissue compared to Cr alone, especially as dose of ATZ increased. Moreover, as dose of Cr in the mixture was increased, the ROS, AhR, CAT, GSH activity was increased compared to low Cr values. However, SOD activity showed reduction in elevation of mixture concentrations. The results of this study suggest that there is synergic impact of co-exposure to ATZ and Cr on the parameters used as markers for liver toxicity in the current study. Further studies are warranted to investigate the interact of Cr and ATZ toxicity in more detail and to avoid having the Cr supplement where ATZ exposure is identified.


Article
Evaluation of Protective Effect of Different Doses of Terminalia arjuna Bark Ethanolic Extract on Cisplatin Induced Oxidative Nephrotoxicity in Rats
Evaluation of Protective Effect of Different Doses of Terminalia arjuna Bark Ethanolic Extract on Cisplatin Induced Oxidative Nephrotoxicity in Rats

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Abstract

Cisplatin (CP), a platinum compound, is one of the most active cytotoxic drugs used for cancer treatment. Nephrotoxicity is severe dose limiting side effect of this drug. Abnormal production of reactive oxygen species (ROSs) leading to oxidative stress has been implicated in kidney toxicity by Cisplatin. Here the study was aimed to evaluate nephroprotective effect of ethanolic extract of Terminalia arjuna bark (EETAB) at the doses (200 & 400 mg/kg, body weight) against Cisplatin (7.5 mg/kg, i.p) induced nephrotoxicity in rats. The evaluation was done by measuring % change in body weight, renal function tests such as Blood Urea Nitrogen (BUN), Serum Creatinine (Cr), Serum Total Protein (TP) and also Kidney SOD (Superoxide dismutase),CAT (Catalase), GSH (Reduced glutathione) and MDA (Malondialdehyde) levels altered by Cisplatin administration. Rats treated with EETAB2 (400mg/kg) significantly (P<0.001) reduced the elevated levels of BUN, Cr, TP, MDA and significantly (P<0.001) increased the levels of SOD, GSH, and CAT by restoring kidney architecture. In conclusion EETAB2 (400mg/kg) attractively showed the protection against Cisplatin nephrotoxicity.

Cisplatin (CP), a platinum compound, is one of the most active cytotoxic drugs used for cancer treatment. Nephrotoxicity is severe dose limiting side effect of this drug. Abnormal production of reactive oxygen species (ROSs) leading to oxidative stress has been implicated in kidney toxicity by Cisplatin. Here the study was aimed to evaluate nephroprotective effect of ethanolic extract of Terminalia arjuna bark (EETAB) at the doses (200 & 400 mg/kg, body weight) against Cisplatin (7.5 mg/kg, i.p) induced nephrotoxicity in rats. The evaluation was done by measuring % change in body weight, renal function tests such as Blood Urea Nitrogen (BUN), Serum Creatinine (Cr), Serum Total Protein (TP) and also Kidney SOD (Superoxide dismutase),CAT (Catalase), GSH (Reduced glutathione) and MDA (Malondialdehyde) levels altered by Cisplatin administration. Rats treated with EETAB2 (400mg/kg) significantly (P<0.001) reduced the elevated levels of BUN, Cr, TP, MDA and significantly (P<0.001) increased the levels of SOD, GSH, and CAT by restoring kidney architecture. In conclusion EETAB2 (400mg/kg) attractively showed the protection against Cisplatin nephrotoxicity.


Article
- Studying the relationship between oxidative stress malondialdehyde and ß-carotene in the serum of asthmatic patients in Basrah Governorate-Iraq
دراسة علاقة إجهاد التأكسد المالونداي الديهايد مع بيتا – كاروتين في مصل دم مرضى الربو في محافظة ألبصره – العراق

Author: S. Gh. Sayyah ساهرة غريب صياح
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2011 Volume: 37 Issue: 1A Pages: 58-69
Publisher: Basrah University جامعة البصرة

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Abstract

The present study was designed to evaluate the relationship between ß-carotene as antioxidant with malondialdehyde (oxidative stress) in the serum of asthmatic patients. In this study, a total number of (50) asthmatic patients (24 males, 26 females) visiting Allergic and Asthma Medical Center in Basrah city were involved and according to age, sex, severity, smoking and family history. From each patient serum blood samples were collected to estimate the malondialdehyde (MDA) and ß-carotene. In addition, (50) healthy subjects were investigated as a control group. We found that a highly significant increase lipid peroxidation measured as MDA (p< 0.01) for all asthmatic patients as compared to healthy control. Also the (MDA) serum level increases significantly (p< 0.05) with age and severity, while a highly significant (p< 0.01) is with smoker patients and positive family history. The study also revealed a statistically significant decrease (p< 0.01) in ß-carotene serum level in all asthmatic patients as compared to healthy control. In regard to age and severity, serum ß-carotene level decreased significantly (p< 0.05) while with smoker patients and positive family history a highly significant decrease (p< 0.01). There were no significant changes in the (MDA) and ß-carotene levels between male and female patients. Moreover, a negative correlation was also observed between ß-carotene with (MDA) level in the patient group with these variables. In conclusion, asthmatic patients suffer a high degree of reactive oxygen species (ROS) formation causing considerable oxidative stress indicated by high level oxidant (MDA) and low level of the antioxidant ß-carotene.

صممت الدراسة على أساس تقييم العلاقة بين بيتا- كاروتين كمضاد للاكسدة مع المالونداي الديهايد (فرط الاكسدة) لمصل دم مرضى الربو, حيث تضمن البحث دراسة (50) حالة مرضية (24 ذكور, 26 إناث) حيث تم جمع العينات من مركز الحساسية والربو في محافظة ألبصره – العراق. تم تقسيم المرضى على وفق مجموعة متغيرات تشمل العمر‚الجنس‚ شدة المرض‚ التدخين‚ والتاريخ العائلي. مقارنة مع (50) حالة للأصحاء (24 ذكور, 26 إناث) كمجموعة سيطرة. أظهرت نتائج الدراسة ارتفاعا معنويا في بيروكسيد الشحوم (فرط الأكسدة) المقاس كمالونداي الديهايد في مصل دم مرضى الربو ( (p< 0.01 مقارنة مع الاصحاء.كذلك ارتفع مستواه معنويا (p< 0.05) بتقدم العمر وزيادة شدة المرض. بينما مع المرضى المدخنين والذين لديهم تاريخ عائلي موجب يكون (p< 0.01) .كما أظهرت النتائج انخفاضا معنويا في مستوى بيتا – كاروتين كمضاد للأكسدة (p< 0.01) في مصل دم مرضى الربو مقارنة مع الأصحاء. ويزداد هذا الانخفاض معنويا (p< 0.05) بتقدم العمر وشدة المرض‚ بينما مع المرضى المدخنين والذين لديهم تاريخ عائلي (p< 0.01). علاوة على ذلك لوحظ ارتباط سالب بين مستوى بيتا – كاروتين مع مستوى المالونداي الديهايد في مجموعة المرضى مع هذه المتغيرات. نستنتج من خلال نتائج هذه الدراسة بان عند المرضى المصابين بالربو درجة أعلى من تكوين الأنواع النشطة للأوكسجين والتي تسبب إجهادا مؤكسدا وزيادة في بيروكسيد الشحوم مصحوبا بتناقص في مضادات الأكسدة مثل بيتا – كاروتين.


Article
4-Hydroxy-2-Nonenal, Indused Nitric Oxide Synthase Statues in Hypertension Patients

Authors: Ali D. Hammed --- Alaa H. Jawad --- Perry H. Saifullah
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2017 Volume: 20 Issue: 3 Pages: 6-11
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The oxidative stress is one of the main cause for cardiovascular diseases (like Hypertension) also one of the results of these diseases. This study involved 56 subjects matched ages and sex divided into two groups; 28 hypertensive subject and 28 healthy subject as control group. The following analysis was done: 4-Hydroxy-2-nonenal (4HNE), Induced nitric oxide synthase (iNOS) and albumin. The results show that there is a significant increase in (4HNE) between patients group and control group. INOS was significantly higher in patients compared to controls while there were no significant difference found in albumin between patient and control group. The increase in 4HNE which is a product of lipid peroxidation is attributed to destruction in body cell caused by due to the increase in stress events. And the increase in iNOS because iNOS produces large amounts of NO as a defense mechanism in response to cytokines and is an important factor in the response of the body to attack by parasites, bacterial infection, and tumor growth. It has been concluded that is important nappy on ideal weight, because obesity considered main factors for heart disease and hardening of the arteries. There is a positive relationship between oxidation results from hypertension and their developments. The aim of this study was to investigate the relationship between of some oxidative stress markers and cardiovascular diseases


Article
The Effect of Regular Exercise Training on Serum Level of Malondialdehyde
تأثير التدريب التمرين المنتظم على مستوى مصل Malondialdehyde

Author: Tayfoor Jalil Mahmoud
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2009 Volume: 13 Issue: 1 Pages: 6-11
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objectives: Poly unsaturated fatty acids(PUFAs ) that contain two or more double bonds are particularly susceptible to oxidation by free radicals and other highly reactive species. Malondialdehyde( MDA) is one of many low molecular weight endproducts of lipid hydroperoxide decomposition and is the most often measured as an index of lipid peroxidation. The aim of the present study was to measure the serum level of MDA in healthy non athletics and athletics.Methods : This study was carried out during the period from April 2007 to September 2007 on 53 healthy non athletics (27 males and 26 females), and 31 healthy athletics (16 males and 15 females). Serum MDA level was measured colorimetrically using thiobarbituric acid method .Results: The mean value of serum MDA was significantly higher in healthy athletics than that of healthy non athletics (p<0.05). The mean value of serum MDA in females was non significantly higher than that of males (p>0.05) in both groups . The mean value of serum MDA was significantly higher in healthy non athletic smokers than that of non smokers (p<0.001) .Conclusions: Based on the findings of the present study , it can be concluded that regular exercise training causes excess lipid peroxidation and generation of significant amounts of MDA, one of the most important harmful free radicals .Therefore athletics should take a diet rich in antioxidants or appropriate amount of antioxidant vitamins ( A, C,and E) .


Article
Estimation and Evaluate of uric acid , Cholesterol and Triglycerides Levels in Hypertension patients in Basra Governorate – Iraq

Authors: Haneen S. Khalil --- Sahera Gh. Sayyah
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2019 Volume: 45 Issue: 1A Pages: 32-44
Publisher: Basrah University جامعة البصرة

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Abstract

The study included the measurement of the mean serum Uric acid, Cholesterol, Triglycerides levels in hypertension patients in Basra Governorate – Iraq. In this study a total number of (80) hypertensive patients (41 males, 39 females) classified according to (age, sex, family history, severity and smoking) as compared with (60) healthy (34 males, 26 females) subjects were investigated as control group. It was found that a highly significant increase is in uric acid, triglycerides and cholesterol in the blood of hypertensive patients as compared with healthy subjects. In accordance with age, severity, family history and smokers. There were significant changes in these variables between males and females. In conclusion hypertensive patients suffer a high degree of reactive oxygen species (ROS) formation causing considerable oxidative stress indicated a high levels of the serum uric acid, triglycerides and cholesterol


Article
Malondialdehyde Level in the Patients Subjected to Open Heart Surgery in Association with Lipid Profile

Authors: Muntaha Abdulmaged Arif --- Mutaz Sabah Ahmeid --- Salih A. Allaw
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2019 Volume: 18 Issue: 1 Pages: 30-35
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Reactive oxygen species are usually produced by the living cell and have different functions in its normal activity and considered as one of the factors that involved in heart disease. Malondialdehyde (MDA) considered as one of the most indictors of oxidative stress and damage produced as a result of lipid peroxidation. Objectives: The main objective is to evaluate serum oxidative stress by measuring the changes in the level of MDA as a marker of oxidative stress and demonstrate the correlation of MDA with lipid profile in patients subjected to open‑heart surgery. Methods: A case–control study was carried out in the Department of Chemistry and Biochemistry, College of medicine,Tikrit University, Tikrit, Iraq. The study was carried out for 50 patients subjected to open‑heart surgery recruited from the Medical City/IraqiCenter for Heart Disease and Ibn Al‑Bitar Cardiac Surgery Center, Department of Cardiac Surgery, Baghdad, Iraq between October 1, 2017and March 1, 2018. The levels of MDA and lipid profile were measured in the serum of 50 patients, in three different interval preoperative, early postoperative, and late postoperative and compared with 30 age‑ and gender‑matched controls. Results: The results revealed a statistically significant difference in the serum MDA level between patient during preoperative, early postoperative, and late postoperative stage against control group and significant differences in their MDA levels among all patients’ stages. Furthermore, there was a significant positive correlation between serum MDA level and total cholesterol in the early postoperative stage. Conclusions: There was an increase in the level of MDA in the early postoperative stage as an indicator of reperfusion damage that occurs immediately after open‑heart surgery which then decreases dramatically with decrease in total cholesterol, triglyceride, low‑density lipoprotein‑cholesterol (LDL‑C), and very‑LDL‑C.

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