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Article
AGE-RELATED CHANGES IN HUMAN SKIN: HISTOLOGICAL, MORPHOMETRIC AND IMMUNCYTOCHEMICAL STUDY USING S100

Authors: Salih S Kadhim صالح سلمان كاظم --- May F Al-Habib مي فاضل الحبيب
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 2 Pages: 111-118
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Aging has many effects on a person’s skin, from wrinkles and sags to increased risk of certain skin conditions, such as skin cancer. As people age, their skin begins to change due to environmental, genetic, nutrition and other factors.Objectives: Understand some of the changes that occur in aging skin including changes in the general morphological, histological and architectural arrangement, epidermal thickness, basement membrane and histochemical changes in melanocytes.Methods: Skin specimens were taken from the anterior abdominal wall of 30 human males at different ages. General histological preparation for paraffin blocks was performed and the blocks were sectioned at (5-6µ) and stained with H&E. S100 protein was used to demonstrate immunohistochemistry labeled melanocytes changes with age. Histometric measurement of epidermal thickness and basement membrane thickness, using eyepiece graticule was performed on these groups.Results: The young age group showed a uniform arrangement of cells in all stratums of the epidermis while the old age group showed diminished thickness of the epidermis. A significant difference between young- adult age group (A and B groups) and the old age group (C group) was recorded. The epithelial basement membrane thickness was increased with age significantly (P value ≤ 0.001). Melanocytes demonstration using S100 showed that these cells tend to be situated at the tips of rete pages, there number are generally low and didn't varied a lot between young and adult age groups. There was yet marked decline in the number of melanocytes in old age group.Conclusion: Aging as a process have a marked influences on skin morphology, thickness, cellularity and basement membrane.Key Words: Aging, skin, S100, morphometry and basement membrane


Article
Assessment of the S100 Protein of the Common Peroneal Nerves in Diabetic Patients

Authors: Payman Jamal --- Talib Jawad Kazim --- Hewa Banna
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 126-133
Publisher: Diyala University جامعة ديالى

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Abstract

The present work designed to study the Immunohistochemical changes of the common peroneal nerves of 30 diabetic patients (8 females and 22 males), their age ranging between (55-75) years and identical samples from 30 un diabetic cadavers (4 females and 26 males) newly imported to department of forensic medicine, at age of (25-50) years as control group. The multiple regression analysis showed significant effect, of age, male gender and duration of diabetes on the development of neuropathy. The protein S100 was significantly depleted in common peroneal nerves samples taken from diabetic patients. The male gender, age progression and to a lesser extent the duration of the disease observed to be risk factors aggravating the depletion of the S100 protein. The present study concluded that neurological factors were simultaneously responsible for the pathogenesis of diabetic peripheral neuropathy, and the age, male gender and a lesser extent the diabetic period may act as risk factors.


Article
Immunohistochemical Distribution of Prolactin Secreting Cells and Anti-S100 Cells in the Pituitary of the Rabbit Oryctolagus cuniculus and Guinea pig Cavia porcellus
التوزيع الكيمياء نسجي مناعي للخلايا الفارزة للبرولاكتين والموجبة لبروتينOryctolagus cuniculis في الغدة النخامية للارنب S-100 Cavia porcellusمم وخنزير غينيا

Author: Daad A. Al-Sinjary¹, Hussain A.M. Dauod² , Kawkab S. Al- Kaisy³
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2013 Volume: 12 Issue: 2 Pages: 48-53
Publisher: Baghdad University جامعة بغداد

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Abstract

Abstract: The objective of the current study was to determine the immunohistochemical distribution and density of prolactin secreting cells and Anti-S100 cells of the pituitary gland in rabbit, Oryctolagus cuniculus and guinea pig, Cavia porcellus. The Immunohistochemical study indicated that prolactin-secreting cells were arranged in single, double or triple groups in the rabbit pituitary gland while they were mostly single cells in guinea pig. In both animals PRL-secreting cells were polymorphic in shape with centric or acentric nucleus. The Anti S-100 cells were scattered in pairs distalis. The S-100 positive cells sometimes had cytoplasmic processes between endocrine cells. Although PRL-secreting cells represent high percentage (44%) in the rabbit. There was no significant increase with t-value 1.11 and the probability was more than 0.05. Anti S-100 cells significally increased (54%) in guinea pig with t-value 2.46 as compared with the rabbit and the probability less than 0.05.

الخلاصة:هدفت الدراسة الحالية لاحتساب توزيع وكثافة الخلايا الفارزة للبرولاكتين و الموجبة لبروتين oryctolagus cuniculus في الغدة النخامية لكل من الارنب وخنزير غينا. لقد اشارت الدراسة الى ان الخلايا الفارزة للبرولاكتين تنتظم على شكل تجمعات احادية وثنائية وثلاثية في الغدة النخامية للارنب بينما تنتظم على شكل تجمعات احادية غالبا في الغدة النخامية لخنزير غينيا. وكانت الخلايا الفارزة للبرولاكتين في كلا الحيوانين متعددة الشكل مع نواة مركزية او غير مركزية و كانت الخلايا الموجبة لبروتين S-100 لكلا الحيوانين منتشرة في الجزء القاصي والحدبي ولقد لوحظ ان الخلايا الموجبة لبروتين S-100 تمتلك احيانا بروزات سايتوبلازمية تمتد بين الخلايا الافرازية على الرغم من ان الخلايا الفارزة للبرولاكتين شكلت النسبة الاعلى في الارنب الا ان الخلايا الموجبة لبروتين S-100 قد ارتفعت معنويا في خنزير غينيا عند مقارنتها بالارنب.


Article
S100A4 and S100A6 Proteins Expression Promote Migration of Bladder Cancer Cells in Zebrafish

Author: Hanaa Al‑Mahmoodi, Ibtihal Alshamarti1, Qais Ibraheem Al‑Ismaeel2, Ahmed Mohamed Salih3, Hishyar Azo Najeeb4, Rusul Mohammed Al‑Rubaay5
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2019 Volume: 16 Issue: 3 Pages: 192-198
Publisher: Babylon University جامعة بابل

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Abstract

Background: Bladder cancer (BC) is one of the major causes of cancer‑related mortality in the world. S100 family of small Ca‑bindingproteins has been implicated in the progression of different cancer types, including BC. Objective: The goal of this study is to develop a morerigorous understanding of the role of S100 proteins in BC progression and also to what extent S100s have the ability to increase the metastaticpotential for BC. Methods: A selected panel of bladder cell lines (J82, T24, HT1376, and RT112) was transplanted into zebrafish embryosto investigate their migration behavior and metastatic potential. Characterizing the expression pattern of S100 proteins including (S100A4and S100A6) in different BC cells was performed using quantitative polymerase chain reaction and Western blot. Assess the effect of S100proteins on bladder cell migration in vivo was carried out using a zebrafish xenotransplant model. S100 proteins expression was modulated bysmall‑interfering RNA approach. Results: High expression of mRNA and proteins levels of S100A4 and A6 were detected in T24 and J82 celllines, which displayed the highest migration rate in zebrafish embryos. In addition, our data showed that the average migration rate of T24 cellstransfected against S100A4, S100A6, and S100A4 and A6 were 17.3%, 10%, and 17.3%, respectively, which was lower than from siControl41.9%. Likewise, the same effect of protein silencing on cell migration was observed in J82 cell lines. S100A4, S100A6, and S100A4 andA6 knockdown reduced the migration rate of J82 cells to 14.6%, 17%, and 14% compared to the control 37.8%. Furthermore, overexpressedS100A4 expression in RT112 cells significantly increased in migration ability by 29%, compared to control cells 7.3%. Conclusion: S100A4and S100A6 play a role in BC cells invasion and migration. Silencing of these proteins affected dissemination of BC cells in zebrafish embryoshighlighting their role in tumor progression.

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