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Article
EFFECT OF NITRIC OXIDE DONOR SODIUM NITROPRUSSIDEON SPERM VOLUME OF DILUTED BULL SEMEN
تاثير واهب اوكسيد النتريك صوديوم نايتروبروسايد (SNP) في حجم النطفه لمني الثيران المخفف

Author: Eman Rassol abd Alshaty ايمان رسول عبد الشاطي
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2012 Volume: 11 Issue: 1 Pages: 355-363
Publisher: Basrah University جامعة البصرة

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Abstract

Study the effect of nitric oxide donor on sperms membrane integrity and volume and their relationship with viability and sperm motility. This study was done usingtwo groupseach one contained 10 samples first one exposed to eight different gradient of hypotonic solutions containing Sodium nitroprusside (SNP)and second 10 samples diluted with gradient hypotonic solutions without Sodium nitroprusside,Bull semen tris dilution treated with Sodium nitroprussidein that protected the sperm fromosmocellular changes stress. The results showed tolerance sperm to gradient hypotonic solution in sperm swelling and classic spermatocrit marked significance by the relative volume shift volumetric data. In addition the SNP had sperm protection to osmolarity tested and give improvement viability and sperm motility. Hypotonic media tonicity that may be attributed to direct liberation of Nitric oxide that produced vital regulation of Na-K ATPase and Calcium channels of sperm membrane

Keywords

nitric oxide --- SNP --- osmolarity


Article
Association of Androgen Receptor Gene Polymorphisms at three SNPs and their Haplotypes with Severe Oligozoospermia Risk in Iraqi Patients

Author: Mustafa F. Khadhim, Ismail A. Abdul-Hassan
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 1 Pages: 16-28
Publisher: Baghdad University جامعة بغداد

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Abstract

Infertility in otherwise healthy males be caused by a variety of disorders. These include acquired, as well as heritable conditions. Genetic causes of male infertility are of special interest to reproductive biology, because these disorders can be passed on to the offspring, single nucleotide polymorphisms are considered as one of reason of male infertility Androgens are critical steroid hormones that determine the expression of the male phenotype. Their actions are mediated by a single androgen receptor (AR) which, upon ligand binding, translocate to the nucleus to regulate the expression of androgen-responsive genes. Mutations in androgen receptor gene may lead to a disturbance in the function of the androgen receptor which, in turn, can lead to several forms of infertility. The aim of this study was to determine the frequency of rs962458(SNP1), rs6152(SNP2) and rs2361634(SNP3)and test their associations with severe oligozoospermia risk .This study was conducted using 50 severe oligozoospermia patients and 50 apparently healthy subjects (Control). The frequency of GA genotype of SNP2 was significantly (p≤ 0.05) increased in patients versus control (10 and 0%, respectively, OR=0.561, X2=4.329),while the frequency of GG genotype of SNP2 was significantly (p≤ 0.01) decreased in patients versus control(84 and 100%, respectively, OR=0.871, X2=6.524). The results of TAA and TAG haplotypes were not significant. The frequency of TAG/TAG haplotype combination was significantly (p≤ 0.01) decreased in patients versus control(78 and 100%, respectively, OR=1.072, X2=8.35),while TAG/TAA haplotype combination frequency was significantly (p≤ 0.05)increased in patients versus control(14 and 0%,respectively, OR=0.664,X2=4.69). In conclusion, both GA genotype of rs6152A>G and TAG/TAA haplotype combination may be correlated with severe oligozoospermia risk in Iraqi patients.

Keywords

SNP --- Androgen receptor --- PCR --- Infertility.


Article
Association of C-allele carrier Genotype of SLCO1B1 gene 521T

Author: Ali Haider Dirjal1, Da'ad Ali Hussain1, Ahmed Salih Sahib2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 237-241
Publisher: Baghdad University جامعة بغداد

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Abstract

The gene SLCO1B1 521 T>C is a crucial gene single nucleotide polymorphism (SNP) concerning of a several medications transport enzyme OATP1B1 action variation such as statins drugs that may cause a serious side effect (myopathy), Iraq lack from recent studies about this variation related to statins side effect myopathy.The study of the SLCO1B1 gene 521 T>C polymorphism effect on Iraqis patients taken statins differ in showing myopathy as a side effect of statin. Settings and Design considered An only treated case-control study. The study of the effect of T521C of SLCO1B1 gene polymorphism and myopathy carried by collect venous human blood (3) ml of (96) samples divided to (48) as control and (48) as case. DNA extraction carried by (wizibiosolutions Korean kit for DNA extraction) and detection by Gel-Electrophoresis, nanodrop device used to adjust purity and concentration proper to next step, TaqMan Real-Time Polymerase Chain Reaction (TaqMan RT-PCR) carried by Real-Time PCR System . Myopathy determined by a history of the patient, clinical examination. Highly significant difference p-value (0.0001>0.01) in genotype carry C-allele between control and case. Genotype (CC) was zero in control compared with cases, all (CC) genotype 5 patients with myopathy was (100%) in case. Genotype (TC) only 5 patients represent (15.6%) was in control parallel to 27 patients represent (84.4%) in case. Genotype (TT) wild type was 43 patients (72.9%) in control compare to only 16 case patients represent (27.1%). All numbers refer to a strong correlation between genotypes carry C-allele (100%, 84.4%) and case (patients with myopathy) against control only (15.6%) of (TC) genotype without myopathy. The significant risk of myopathy of (CC) genotype compare with (TT) genotype when (n=96) odd ratio (17.2) at (95%) Cl between (5.7051 to 51.8551) and P (0.0001) indicate the risk of development of myopathy for C-allele carrier genotype. C-allele considered a risk factor for patients taken statin lead to develop of myopathy in Iraq.


Article
Effect of MDR1 Gene Expression Related with C1236T Polymorphism in Iraqi Acute Myeloid Leukemia patients
تأثير تعبير جين المقاومة المتعددة MDR1 وعلاقته مع تعدد طرز الموقع C1236T في مرضى ابيضاض الدم النخاعي الحاد العراقيون

Author: Abdul Hussein M. AL-Faisal 1 Kifah Jabbar Alyaqubi 2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2014 Volume: 13 Issue: 2-2 Pages: 253-265
Publisher: Baghdad University جامعة بغداد

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Abstract

Single nucleotide polymorphism (SNP) in multidrug resistance gene1 (MDR1) could alter the gene expression level and may have effect role in responses to drug therapy and diseases susceptibility. The aim of the present study is to investigate allele frequency in Iraqi healthy and acute myeloid leukemia (AML) patients to detect the susceptibility of C1236T genotype carrier to develop AML. Also the study aimed to correlate the expression level of MDR1 mRNA with MDR1 C1236T polymorphism in newly diagnosed AML patients to predict clinical outcomes at initial diagnosis. The results showed there was a significant difference in genotype and allele frequency with heterozygous CT in AML while appeared significant with CC wild type in healthy control. Both AML and control showed non-significant in allele frequency. In regard with gene expression the healthy control showed significantly high level of MDR1 mRNAs expression in CC genotype at position 1236 compared with CT and TT. Whereas MDR1 heterozygous 1236CT genotype was showed highly significant difference in MDR1 mRNA expression among AML patients. According to the clinical outcome status MDR1 C1236T genotype showed statistically high significant differences with low level of CC genotype compared to CT/TT genotype at initial diagnosis in NR patients, while CR group was showed non-significantly with MDR1 C1236T polymorphism. In conclusion healthy Iraq populations have predominantly CC genotype and appeared a protective genotype, while MDR1 1236CT/TT genotype were indicated affected genotype and associated with poor prognosis in de novo AML patients, while CC appeared good prognosis.

ان تعدد طرز النيوكليوتيدة المفردة SNP في جين المقاومة المتعددة MDR1 قد يؤدي الى تغيير تعبير الجين MDR1 مما له تأثير على الاستجابة للعلاج الكيميائي وعلى حساسية المرض. أن هدف هذه الدراسة هو تحديد تكرار اليلات الجين في مرضى ابيضاض الدم النخاعي الحاد العراقيون ولمعرفة حساسية الطراز الوراثي للموقع C1236T وعلاقته بالإصابة بالمرض. كما هدفت الدراسة الى ايجاد العلاقة بين مستوى تعبير جين المقاومة المتعددة و طرزه الوراثية في المرضى المشخصين حديثا للاستفادة منها في التشخيص والتوقع الطبي للمرض. بينت الدراسة بوجود اختلاف معنوي في المظاهر الوراثية و تكرار الاليلات مع الزيجة المغايرة للطراز الوراثي MDR1 C1236CT في المرضى مقارنة مع معنويه في الطراز الوراثي MDR1 C1236CC في الاصحاء. كما بينت النتائج بأن كلا المرضى والاصحاء بينوا عدم معنوية تكرار الاليلات. بينما كان تعبير الجين MDR1 معنوي عالي في طرازه الوراثي MDR1 C1236CC مقارنة مع طرزه الوراثية MDR1 C1236CTوMDR1 C1236TT عند الاصحاء بينما كان المظهر الوراثي مغاير الزيجةMDR1 C1236CT عالي المعنوية عند المرضى. واستنادا للتوقع الطبي للمرض فأن الطراز الوراثي MDR1 C1236CT كان مختلف معنويا وعاليا مع انخفاض الطرز الوراثية MDR1 C1236CC و MDR1 C1236CCTT عند التشخيص الاول للمرض. بينما كانت مجموعة ذات تعدد طرز وراثية غير معنوي. وكخلاصة فأن تكرار الطراز الوراثي MDR1 C1236CC شائع لدى الاصحاء العراقيون ويبدو انه مرتبط مع الحماية بينما تمثل الطرز MDR1 C1236CTTT الطرز المتأثرة والتي ترتبط مع تشخيص ضعيف للمرض مقارنة مع الطرازMDR1 C1236CC الذي يمثل طراز جيد لتشخيص المرض.

Keywords

C1236T --- MDR1 --- AML --- Genotype --- SNP --- Iraq.


Article
Association Between Polycystic Ovary Syndrome and Genetic Polymorphisms of CYP 17 Gene in Iraqi Women

Author: Marwa B. Mohammed1, Salwa J. AL-Awadi2, Mahfoodha A. Omran3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2015 Volume: 14 Issue: 2 Pages: 99-110
Publisher: Baghdad University جامعة بغداد

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Abstract

Polycystic ovary syndrome (PCOS), whose genetic basis is not completely well understood, it is the most common endocrine disorder in women in their reproductive years. Blood samples were collected from two groups. The first group included 61 females with PCOS and the second group included 30 normal females to detect the presence of mutation in the CYP17 gene. The two groups were genotyped and a comparison was done between them. The results showed a significant difference (p<0.05) in FSH levels in patients and normal females and there is no significant difference in the levels of Testosterone, prolactin and LH. Moreover, it showed a significant difference (p<0.05) in the levels of HDL in both patients and normal females and no significant difference in the levels of (LDL, VLDL, Triglyceride and cholesterol). Two comparisons for genotype were done: one between age and genotype and the second between BMI and genotype for each group. The results showed two types of genotype, which were a TT wild type and a heterozygote TC mutant type. Furthermore, the results showed a significant difference (p<0.05) in genotype TT and TC in the group of age less than 25 years old and no significant difference in these genotypes in age groups (25-35) and those with more than 35 years old. It is concluded from this study that this single nucleotide polymorphism in the CYP 17 gene was not associated with PCOS in Iraqi women.

Keywords

PCOS --- BMI --- CYP17 --- SNP --- RFLP --- genotype.


Article
Study the effects of STRA 6 gene polymorphism on the incidence of T2DM in a sample of Iraqi patients

Author: Sara N. Obaid , Basima Q. AL-Saadi
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 9-17
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was aimed to detect STRA6 gene polymorphism in Iraqi type 2 diabetes mellitus patients (T2DM) to found the correlation between the SNP (rs 736118) polymorphism in STRA6 gene and lipid metabolism and impact on the incidence of type 2 diabetes mellitus (T2DM) . Genomic DNA was extracted from the blood samples of T2DM patients and apparently healthy as a control group by using genomic DNA purification kits (Geneaid), the purity of the DNA was between 1.7 -1.9 and the concentration between 30 -100 ng / μl,. total cholesterol TC , high density lipids HDL, low density lipids LDL and Triglycride and fasting blood sugar FBS were measured by kits supplied by (Biolabo, France), while glycosylated hemoglobin HbA1C measured by kit supplied by (Nycocard, Norway). Mean value of triglycerides in diabetic patients was significantly (p<0.001). The result show high Significant difference was observed in FBS level (p<0.001) in patient group (183.47 ± 6.32) and in control (88.61 ± 3.70), in other hand high Significant difference was observed in HBA1C level was (p<0.001) in patient group ( 8.87 ± 0.21 ), while in control group was (4.86 ± 0.10). Cholesterol mean level value in diabetic patients was significantly higher than those of control group (p<0.001) , LDL-Cholesterol mean value in diabetic patients was statistically significant (p<0.005) higher than the mean value of control group, (p<0.001), Serum HDL-Cholesterol mean value was significantly (p<0.001), VLDL-Cholesterol mean value in diabetic patients was significantly (p<0.001) increased compared to the mean of control group. Real time PCR (Taq man ) RT-PCR were used to detect SNP (rs 736118) C>T in STRA6 gene by using specific probes and primers, As a related with SNP (rs 736118) C>T in STRA6 gene , the percentage of those CT polymorphism genotype in the patients group was significant (p<0.001) compare with control group (58% versus 0% respectively) and the individuals with the CT genotype in the patients group is significantly higher than those with CC genotype, the percentage of those with TT polymorphism genotype in the patients group is significantly higher as compared with control group (6% versus 0% respectively . The percentage of those with CC polymorphism genotype in the patients group show highly significant differences as a compared with control healthy group (28%, 0% respectively), as a conclusion of this study there was a relationship between polymorphism of STRA 6 gene SNP (rs736118) and the incidence of T2DM in sample of Iraqi patients.


Article
Influence of multi-drug transporter gene ABCG2 polymorphism (C421A) in clinical out care in some Iraqi chronic myeloid leukemia patients treated with imatinib mesylate

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Abstract

In CML patients, Imatinib Mesylate (IM) treatment is a good choice with an excellent efficacy outcome. But unfortunately, a significant obstacle of IM resistance has emerged relating to the genetic polymorphism in drug transporter genes such as ABCG2 which effects the metabolism and pharmacokinetic IM. This study investigate the influence of ABCG2 C421A SNP in IM response among some Iraqi CML Patients (71 patients : 43 Male , 28 Female) aged between ( 20-70 ) years in chronic phase with positive Philadelphia (Ph) chromosome, including 11 newly diagnosed , and 60 treated with standard dose IM (400mg) on frontline treatment , 30 of them were IM response and 30 resistance to IM drug , on the other hand 25 apparently healthy individuals were included as control group. After the patients were informed about the details of the research, they were approved to take samples of their blood for study , 3ml of peripheral blood was withdrawn from CML patients and control groups . The frequency of homozygous mutant genotype (AA) of SNPabcg2 C421Awas significant higher in IM resistant CML patients as compared to IM good response CML patients with (O.R=1.309 and p < 0.01) . The SNP abcg2 C421A was found to contribute to the genetic susceptibility of CML when evaluated with healthy control subjects. These reconnoitering results give a reasonable cause to explore such SNPs to be used as a biomarker in prediction the response to IM treatment before getting started.


Article
Effect of G1705A SNP in Growth Hormone Gene on the Productive and Physiological Performance in Broiler Chicken

Author: Bassam G. M. Al-khatib, Dihya H. H. Al-Hassani
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 1 Pages: 33-45
Publisher: Baghdad University جامعة بغداد

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Abstract

In this study two commercial broiler hybrids were used (Cobb500 and Hubbard-F15) to detect the G1705A SNP in the third intron of chicken GH gene and investigate its effect on the productive and physiological performance in broiler chicken. PCR- RFLP method was used to identify this SNP, three genotypes were found when using of Eco RV restriction enzyme wild genotype GG, heterozygous GA and homozygous AA. Highly significant difference (p<0.01) was found between the distribution of the different genotypes, the genotype GG had the highest percentage followed by GA then AA and allele G had the superiority over allele A in both broiler breed, no significant effect of the various genotypes on the productive traits in both broiler breeds of study, significant effect (p<0.05) of the various genotypes were found on the serum total protein and triglycerides concentrations of Cobb500 at 14 days of age and the genotypes GG and GA were gave highest mean followed by the genotype AA.

Keywords

GH gene --- PCR-RFLP --- Eco RV --- G1705A SNP.


Article
Association between Resisten Gene Polymorphism (420C/G) and Lipid profile in Ischemic Heart Diseases

Authors: Asraa Ali Kadhum --- Majid Kadhum Hussain --- Riyadh. Dh. Al-zubaidi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2018 Volume: 11 Issue: 1 Pages: 3937-3942
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: (resistin is considered as a pro-inflammatory molecule and plays a role in the inflammatory response that lead to atherosclerosis)Aim: To evaluate the risk of resisten gene polymorphism (420C/G) in the development of Ischemic Heart Disease. To verify the relationship of the investigated SNPs with the metabolic changes related to (IHD), in particular, serum lipid profile.Methods: A case control study was performed at which 150 patients with IHD and 150 healthy individuals. Genotyping for SNP 420C>G in the resistin gene was performed by the polymerase chain reaction –restriction fragment length polymorphism method. Lipid profile were measured.Results: The genotype and allele frequencies of resistIn gene polymorphism in IHD and control persons were examined under the co-dominant, dominant and recessive models with the use of multi nominal logistic regression analysis. Neither genotype distribution nor the minor allele frequency showed significant changes among the comparison of the of IHD patients with the control group. The frequency of the G allele of 420(CG) polymorphism was significantly higher in ischemic heart diseases (IHD). There are significant increases in the level of LDL, triglyceride, VLDL, BMI and a significant decrease in the level of cholesterol in the group of patients with the GG+CG genotypes when they were compared with those of the CC genotype. It shows significant differences in BMI, Age, Cholesterol, LDL, and HDL in the group of IHD, and no significant difference was seen in VLDL and sex.Conclusion: The −420C>G SNP of resistin gene is not associated with ischemic heart disease in the population of Kerbala and Najaf. The G allele is seemed to increase serum lipid concentrations so it could be considered as an atherosclerotic parameter.


Article
Genetic Polymorphisms SNP (rs5925) of LDLR Gene Associated with Familial Hypercholesterolemia in Iraqi Patients

Author: Alaa Riehan1 , Wiaam Ahmed Al-Amili 2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 75-81
Publisher: Baghdad University جامعة بغداد

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Abstract

The low density lipoprotein receptor (LDLR) allele status is the predominant hypercholesterolemia genetic risk factor. Functional single nucleotide polymorphisms (SNPs) in human LDLR gene receptors represent an excellent nominee for association with hypercholesterolemia. So, a common SNP ( c.1959T>C ; p.Val653Val , exon 13 , rs5925) in LDLR gene was studied using Real-Time PCR and restriction fragment length polymorphism (PCR-RFLP) techniques to show association between LDLR SNP with Familial hypercholesterolemia . Seventy of Familial hypercholesterolemia patients who were clinically diagnosed by physician and 30 apparently healthy individuals were conducted within this study. Blood samples were collected from all subjects after 12-14 hour fasting. Genomic DNA was extracted from blood samples and analyzed for rs5925 SNP in LDLR gene with specific primers and probes using Real-Time PCR technique. Also, genomic DNA was amplified by conventional PCR with specific primers for detection of this SNP using PCR-RFLP technique. Using two methods in identification of rs5925 SNP for LDLR gene in this study come in different performance success percentage of the methods. Where, the Real-Time PCR gave 100% performance success for all subjects, while PCR-RFLP gene gave only 64% performance success for FH patients and 70% for control group. On the other hand, when the two methods were success to be done they gave fairly close results. In Real-Time PCR and PCR-RFLP, FH patients appeared CC homozygous genotype and TC heterozygous genotype significantly higher than in control group while the control group showed significantly increasing in TT homozygous when compared with FH patients. In comparison of the allele frequencies of C and T of LDLR gene, FH patients showed that the variable allele C was higher than T allele within this group. This association may be observed between allele polymorphism and risk of FH.

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