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Morphometric study of kidney and suprarenal gland development after super ovulation injection in mice

Authors: Mohammad Oda Selman --- Ban Thabit Al-Ani --- Rana R. Al-Saadi --- Usama I. M. AL-Zubaidi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 6 Pages: 49-53
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Superovulation in mice causes a delayed embryonic development in vitro and in vivo, an increased abnormal blastocyst formation, pronounced fetal growth retardation.Objective:Morphometric measurements the dimensions (thickness, length) of fetal kidney and suprarenal gland during the later intrauterine life, after superovulation induction.Methods:Twenty fivematurefemalemiceSwiss-Webstermice ,thesemiceweredividedintotwogroups:experimental (G1) (15animal/group) and control group(G2) (10 animal/group). Superovulation was induced by intraperitoneal (IP) injection of 7.5 IU of PMSG ,then followed by 7.5 IU of HCG after 48 hours. Ovulation was taken place between 122± hours after injection of HCG. One mature male mice was placed with three superovulated female mice for mating. Pregnant females were sacrificedongestational day eighteen in all experimental groups. For morphometric study 20 mice embryos were fixedinBouin>sfixative,longitudinalsectionsofwholeembeddedembryowerestainedbyH&E,thespecimens independently read. The dimensions (thickness, length) of fetal kidney and suprarenal gland were evaluated.Results:The statistical analysis showed significantdecrease(P<0.01)inweightsoffetusesincomparisonwithcontrol group. The study was showed significantdecrease(P<0.01)infetalkidney dimensions atday 18 of intrauterine life, when it compared to the fetal kidney of non superovulated group(control group). There was also significantdecrease(P<0.01) inthemorphometricmeasurementsof fetalsuprarenal gland dimensions.Conclusion:The study improve that there is a delay in growth of kidney and suprarenal gland in fetal mice that belongs to mother undergo to superovulation induction before pregnancy.

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