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Article
Role of TGF-β1 in Urinary Bladder Carcinoma
دور عامل النمو المتحول TGF-β1) ) في سرطان المثانة

Authors: Munther J. Hussain منذر حسين الكاظمي --- Amna N. Jassem أمنة نصيف جاسم --- Hind M. Mousa هندمزهرموسى
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 4 Pages: 1481-1485
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study aimed to evaluate sera TGF- β1 concentration in patients with urinary bladder carcinoma (UBC). All malignant of them was transitional cell carcinoma (TCC) type , patients with urinary bladder disorders (UBD ) and healthy control , and to study the correlation between sera TGF-β1 levels and tumor stages and grades in UBC patients . A direct ELISA test was used to quantify the seraTGF-β1 concentrations in sera of 58 patients with urinary bladder carcinoma UBC of different grades (G) and stages (T) all malignant of them was transitional cell carcinoma (TCC) type , 15 from patients with UBD and 15 healthy subjects . Sera levels of TGF-β1 were elevated in patients with UBC and UBD compared to healthy (P ≤ 0.01) .There was no association between tumor stages and serum level of TGF-β1 (P˃0.05) . Whereas there was an association between serum levels of TFG-β1 and tumor grades (p ≤0.05) . Increased serum level was found in high grades G≥2 of patient with UBC .

هدفت الدراسة الحالية إلى تقدير مستوى تركيز عامل النمو المتحول TGF-β1 في أمصال مرضى سرطان المثانة البولية ومجموعتي (مرضى اضطرابات المثانة البولية والأشخاص الأصحاء)كعينات سيطرة , و دراسة ارتباطه مع مراحل ومراتب مرضى سرطان المثانة البولية . تم استخدام اختبار الاليزا المباشر لتقدير مستوى تركيز البروتين في أمصال ـ58مريض يعانون من سرطان المثانة البولية ضمن مراحل ورتب مختلفة معظم الاورام كانت من نوع سرطان الخلايا الانتقالي الحرشفي ,15مريض يعانون من اضطرابات المثانة البولية و15 أشخاص أصحاء . وقد وجدت الدراسة أن مستوى تركيز بروتين النمو المتحول TGF-β1 في المصل عالي احصائيا في مرضى سرطان المثانة البولية ومرضى اضطرابات المثانة البولية مقارنة بالأشخاص الأصحاء وكانت القیمة الإحصائية (P< 0.01 ) بين المجاميع . وبينت النتائج أيضا عدم وجود علاقة بين مراحل مرضى السرطان وتركيز البروتين وكانت ألقيمه الاحصائية ((P˃ 0.05 في حين أن هناك علاقة بين مراتب مرضى السرطان وتركيز البروتين اذ كانت القيمة الاحصائیة (P< 0.05 )وأظهرت المراتب المتقدمة تركيز عالي للبروتين .


Article
Association of Transforming Growth Factor Beta1 Gene Polymorphism with Diabetes Mellitus Risk in Iraq Patients

Author: Rasha Saleh Nuhiar1 , Ali Naeem Salman2 , Hassan Raysan AL-Rekaby2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 49-56
Publisher: Baghdad University جامعة بغداد

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Abstract

Diabetes mellitus (DM) refers to a group of multifactorial metabolic disorders characterized by elevated blood glucose levels (hyperglycemia) that result from defects in the body's ability to produce and/or insufficiency of insulin action .This study was to investigate the correlation between TGFβ1 polymorphism and Diabetes Mellitus .The present study carried out in the labs of college of education for pure science and Center for Diabetes and Endocrinology of the Health Directorate in Thi- Qar province, the period of research was extended from January- July 2017. To test for the association of Polymorphisms in promoter region (G-800A) and (C-509T ) of transforming growth factor- β 1 (TGF-β1) gene with diabetes mellitus in Iraqian patients. The study included a total of 120 patients with type I and II diabetes and their age between 1-51 years. in addition to 52 healthy controls . DNA has been isolated and RFLP-PCR was performed by using primers specific for genotypes of two region of the TGF β1 gene (C509T)and (G800A). The results showed that only C509T polymorphism of the TGFβ1 gene is significantly different in genotype distribution in allelic frequencies between DM patients and control subjects and association with clinical characteristics. Thus this SNP seems to be related to DM susceptibility. This study supports the involvement of TGFβ1 gene polymorphism in the incidence of DM in Thi-Qar population.

Keywords

Diabetes Mellitus --- TGF-β1 --- G800A --- C509T.


Article
Antibacterial and anti-ulcerogenic effects of Punicagranatum peel extract against ethanol-induced acute gastric lesion in rats

Authors: Sheila M. Nuraddin --- Zahra A. Amin --- Sargul H. Sofi --- Shokhan Osman
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2019 Volume: 23 Issue: 3 Pages: 308-314
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objectives: Punicagranatum (pomegranate) is a fruit-bearing deciduous shrub or small tree from the family Lythraceae. It has been cultivated since ancient times throughout the Mediterranean region. Different parts of it have been used for research, such as fruits, peels, and juice. This study aimed to investigate the antibacterial and anti-ulcer effect of Punicagranatum peel extract and to screen the expression of transforming growth factor β1 (TGF β1) in rat’s blood serum after stomach ulcer was induced by ethanol.Methods: Twenty four rats were divided randomly into 4 groups; GroupI(ulcer positive group) rats were (experimental) orally administered 5 ml/kg sterilized distilled water (the vehicle). Group II (ulcer negative group) rats were orally administered 5 ml/kg of 20 mg/kg esomeprazole. Groups III and groups IV were orally administered 5 ml/kg of 250 and 500 mg/kg of Punicagranatum plant extract, respectively.Results: Data showed an antibacterial activity of Punicagranatum peel extract against gram positive and gram negative bacteria and the best antibiotic in which both bacteria were sensitive to was norfloxacin(10 µg). Treatment with Punicagranatum peel extract and esomeprazole had protective effects on stomach gastric mucosa, which indicate an anti-ulcer effect confirmed by the high levels of TGFβ1 in serum.Conclusion: We conclude that Punicagranatum peel extract exhibit antibacterial effect and possess a protective role against ethanol induced gastric ulcer in rats.


Article
Validity of the Ratio of Serum Concentration of Interleukin 6 to Transforming Growth Factor-Beta1 in a Sample of Patients With Rheumatoid Arthritis

Author: Nizar Abdulateef Jassim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2015 Volume: 14 Issue: 2 Pages: 296-301
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Interleukin 6 (IL-6), a pro-inflammatory cytokine, plays a key role in the development of rheumatoid arthritis (RA). Transforming growth factor-beta1 (TGF-β1) is an anti-inflammatory cytokine which is considered to be an important (down) regulator of inflammation in RA.OBJECTIVE:To evaluate serum concentration of IL6 to TGF-β1 ratio in a sample of Iraqi patients with RA. METHODS: A case control single center study was conducted for 11 months. A total of 50 patients with RA diagnosed according to the 1987 American College of Rheumatology (ACR) and 30 individuals matched in age and sex as control group were included. Serum concentration of IL-6 to TGF-β1 ratio and serum IL-17 were determined in both RA groups using enzyme-linked immunosorbent assay (ELISA). The cut off value was assessed by receiver operating characteristics (ROC) test and correlation by spearman's Rholinear correlation coefficient.RESULTS: Frequency of females was more than males in patients and controls (88% and 76.7% respectively). Ages of patients range between 20-70 years with a median 43.76 years while in controls, ages range between 20-60 years with a median 37.67 years. Serum concentration of IL-6 toTGF-β1 ratio was significantly more in patients than those in controls (p=0.018). Area under the curve (AUC) at value 0.686 was statistically significant (p=0.018) and had intermediate accuracy. Serum IL-6 to TGF- β1 ratio≥ 2.4pg/ml was the optimum cutoff value that can differentiate between RA and healthy controls with accuracy 71.9%. There was a significant positive linear correlation between IL-6 to TGF-β1 ratio and IL-17( r=0.56, p<0.001) and simple linear regression analysis showed that for each 1unit increase in IL6 to TGF-B1 ration there was a significant increase of 0.7 pcg/ml in IL-17(R2=0.467, P < 0.001)CONCLUSION: Serum concentration of IL6 to TGF-β1 ratio was significantly higher in Iraqi sample of RA patients compared to controls. This may help in early diagnosis of RA and suggest potentially an early effective treatment.


Article
Relationship between Transforming Growth Factor-Beta 1 gene polymorphism and hypertension
في الكودون 25 ومرض ضغط الدم TGF-β العلاقه بين الطفره لجين عامل النمو 1

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Abstract

This study intends to evaluate the association between Transforming Growth Factor - Beta1(TGF-β1) SNP codon 25 and hypertension in holy Kerbala city, Iraq. A case control studyfor one hundred and four subjects. Seventy four hypertensive patients (23 male and 51female), already diagnosed with essential hypertension, and 30 control subjects (16 maleand 14 female). The links between genotype and hypertension were examined then possibleSNP related variances in the blood pressure were checked. The present study suggested thatthere is no significant association between transforming growth factor beta one (TGF-β1)and hypertension in its Iraqi population sample. However, there is a significant associationbetween Arginine (Arg25) and hypertension compared with control group clearly shown inthe male gender (p < 0.05). In this study, the associations between the SNP of TGF-β1codon 25 and hypertension was not significant while the SNP showed genotype-relateddifferences in gene allele (Arg25).

المحمول في كودون 25 وارتفاع ضغط الدم TGF-β تعتزم هذه الدراسة إلى تقييم العلاقة بين عامل النمو الجيني 1لعينة بشرية من مدينة كربلاء المقدسة، العراق. تضمنت هذه الدراسه مائه واربعه عينه. اربعة وسبعون من مرضىارتفاع ضغط الدم ( 23 من الذكور و 51 من الإناث)، تم تشخيصهم بارتفاع ضغط الدم مسبقاً، و 30 عينة من الاصحاءذات الصلة SNP 16 ذكور و 14 إناث). تم فحص الروابط بين النمط الجيني وارتفاع ضغط الدم ثم تم فحص الفروق )وارتفاع (TGF-β في ضغط الدم. وأشارت الدراسة أنه لا توجد علاقة ذات دلالة احصائية بين عامل النمو بيتا واحد ( 1وارتفاع ضغط الدم مقارنة (Arg ضغط الدم في عينة السكان العراقية. ومع ذلك، هناك علاقة وثيقة بين أرجينين ( 25.(p< مع مجموعة التحكم يظهر ذلك بوضوح في جنس الذكور ( 0.05


Article
IMMUNOLOGICAL PROFILE IN DIFFERENT GROUPS OF END STAGE RENAL DISEASE
النمط المناعي في مجاميع مختلفة من الطور النهائي للفشل الكلوي

Authors: Madha Mohammed Sheet Saleh مضاء محمد شيت صالح --- Zahraa Ali Ahme زهراء علي احمد
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2016 Volume: 8 Issue: 3 Pages: 245-252
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background and aim of study: End Stage Renal Disease (ESRD) is a worldwide problem in which patients are in hemo-dialysis and/or awaiting for kidney transplantation. However, the actual mechanism (s) of ESRD pathogenesis is ill-defined. The aim of this study is to investigate the role of certain immunological markers in the pathogenesis of ESRD. Materials and methods: Sixty eight blood samples were collected from hospitalized ESRD patients with different etiology (hypertensive, diabetics, hypertensive + diabetics, and small size kidney). Twenty healthy volunteers as control group was enrolled in the study. Serum IL-10, IL-17, MCP-1 and TGF-β were estimated in all subjects. Result: A significant elevation in the serum IL-10, MCP-1 and IL-17 mean concentration in all ESRD patients groups. TGF-β mean concentration exhibited decreasing level in the hypertensive, hypertensive + diabetes and small size kidney groups and a slight elevation in the diabetes group. The IL-10: IL-17 ratio expressed elevation in all ESRD patients groups. Conclusion: There is a progress of inflammatory reactions in all ESRD patients groups in which IL-17 and MCP-1 are playing major roles. TGF-β1 is not played its anticipated pro-fibrotic role and anti-inflammatory function in the studied group. The ratio of IL-10: IL-17 point out a slight shifting of the immunosuppressive reaction over the inflammatory reaction in all ESRD patients groups.

خلفية واهداف البحث: المرحلة النهائية من الفشل الكلوي مشكلة عالمية سببها عدم قيام الكلية بعملها الطبيعي وعلاجها هو الديلزة الدموية او الزرع الكلوي. الالية الدقيقة في نشوء وتطور الجانب الامراضي لهذه المشكلة لا يزال مجهول. هدف الدراسة هو تقصي دور بعض المعلمات المناعية في امراضية هذه العلة.المواد وطرائق العمل: ثمانية وستون عينة دم جمعت من مرضى المرحلة النهائية للفشل الكلوي الراقدين في المستشفيات لاسباب مختلفة (ارتفاع ضغط الدم, السكري, ارتفاع ضغط الدم والسكري في ان واحد, الكلية الصغيرة والاسباب والاخرى). أشرك 20 متطوعا سليما كمجموعة سيطرة في البحث. تم قياس مستوى الانترلوكينات 10 و17 اضافة الى عامل النمو التحولي بيتا (TGF-β) وبروتين-1 االمستدعي الكيمياوي للبلاعم الكبيرة (MCP-1) لجمع الافراد المشاركين بالبحث.النتائج: ارتفاعا مصليا معنويا في المعدل الحسابي لمستويات (IL-10, IL-17 , MCP-1) في جميع المجاميع. من جهة اخرى كان هناك انخفاضا مصليا معنويا في المعدل الحسابي لمستوى (TGF-β) في جميع المجاميع الدراسية ما عدى مجموعة السكري. كان هناك ارتفاعا في ال (IL-10) فاق الارتفاع الحاصل في ال (IL-17) مما ادى الى ارتفاع نسبة ال (IL-10: IL-17) في جميع المجاميع المرضية.الاستنتاجات: وجود تفاعل التهابي لجميع المجاميع المرضية يلعب (IL-17) و (MCP-1) دورا اساسيا. اشارت النتائج ايضا الى عدم لعب العامل المناعي (TGF-β) دوره المتوقع كعامل تليف ومضاد للالتهاب في المجانيع المرضية فيما عدا مجموعة السكري. كانت نسبة العامل (IL-10: IL-17) تشير الى تحول جزئي في التوازن لصالح تفاعلات الكبت المناعي على حساب تفاعلات الالتهاب في جميع المجاميع.

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