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Article
The association between acne vulgaris and Tumor Necrosis Factor- alpha gene promoter polymorphism at position-308

Author: Hamadi Hadi Abttan Al-Hilali حمادي عبطان الهلالي
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2014 Volume: 10 Issue: 17 Pages: 86-95
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Results; genotyping of TNF-α revealed three genotypes; the wild homozygous GG type, the heterozygous GA & the mutant homozygous AA type, the genotype frequency for these three types in acne patients was (29%, 67% & 4%) respectively, in control group, however, it was (58%, 30% & 12%) respectively. The frequency for GA type was found statistically significantly increased in acne patients compared to the healthy controls (P<0.001), significant association was found between the minor A allele and females patients(40.6%) compared to healthy females(28.8%) ,P value 0.001,also all the female that had severe and very severe acne carry the GA genotype. Background ;This study was carried out on (100) patients (64 females & 36 males) with different severity of acne vulgaris with an age range(11-40 )year, seen in AL-Diwaniya Teaching Hospital/ Department of Dermatology, from December 2011 to February 2012, another (50) apparently healthy subjects were taken as a control group. Materials; Blood samples were collected from both groups, genomic DNA was extracted from peripheral blood leukocytes for further molecular study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used .

أجريت هذه الدراسة على (100) مريض من مرضى حب الشباب بمختلف درجات الشدة (64 إناث و 36 ذكور) من الفئة العمرية (11-40 سنه) من مراجعي قسم الأمراض الجلدية في مستشفى الديوانية التعليمي للفترة من كانون الأول 2011 حتى شباط 2012, إضافة إلى (50) شخص سالم ظاهريا كمجموعة تحكم.تم جمع عينات من الدم لاستخراج الحمض النووي من الكريات البيض لكلتا ا لمجموعتين للكشف عن وجود أي ارتباط بين تعدد الأشكال الجينية لعامل نخر الورم-ألفا في الموقع 308 من المنطقة المروجة والاستعداد للاصابة بحب الشباب باستخدام تفاعلات البلمره المتسلسلة وتقنية الأجزاء المتكسرة المتعدد باستخدام إنزيم NCOI والتي تعطي أجزاء ذات حجوم جزيئيه مختلفة تعبر عن الأصناف الجينية المختلفة .أظهرت تقنية الأجزاء المتكسرة المتعدد ثلاثة اصناف جينية لعامل نخر الورم-الفا في الموقع 308 وهي: صنف متماثل اللواقح GG, والصنف المتخالف GA, ونوع متحول متماثل اللواقح AA وكان تردد هذه الأصناف الجينية الثلاثة عند المرضى كالاتي :(29% , 67% و 4%) على التوالي, إما في مجموعة الاصحاء فقد كانت كالاتي :(58%, 30% و 15%) على التوالي.وجدت الدراسه ان هناك فرق احصائي مهم بين الصنف الجيني من نوع GA بين مرضى حب الشباب مقارنة مع الاصحاء P < 0.001)). اضافة الى العثور على ارتباط مهم بين الاليل A عند الاناث المرضى(40.6%) مقارنة بالاناث الاصحا(28.8%) اضافة الى ان جميع النساء المصابات بحب الشباب الشديد وجدن انهن حاملات للنوع الجيني GA.نتيجة هذه الدراسه تشير الى ان تعدد الاشكال الجينيه لعامل نخر الورم من الممكن ان تسهم في زيادة الاستعداد للاصابه بحب الشباب مع ملاحظة وجود ارتباط مهم بين الاليل A الطفيف وشدة المرض عند الاناث.


Article
Detection of Toxoplasma Antibodies and TNF-α in Rheumatoid Arthritis Patients Treated with Methotrexate
التحري عن الاجسام المضادة للمقوسات الكونيدية و عامل التنخر الورمي في مرضى التهاب المفاصل الرثياني المعالجين بالميثوتريكسيت

Authors: Rasha H. Kuba رشا حسين كبة --- Khawla H. Zghair خوله حوري زغير --- Mohammed H. Alosami محمد هادي العصامي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 4A Pages: 1535-1540
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was conducted to evaluate the prevalence rate of toxoplasmosis among 294 rheumatoid arthritis (RA) patients treated with methotrexate (MTX), 50 RA patients without treatment and 50 samples as healthy control. Blood samples were collected and the presence of T.gondii IgG and IgM antibodies was determined by using Enzyme linked immunosorbent assay (ELISA). Tumor necrosis factor alpha (TNF-α) was also estimated in serum of all subjects by using ELISA method too. The seroprevalence of toxoplasmosis IgM and IgG in RA+MTX was 60(20.408%), and 98(33.33%), in RA patients 4(8%), and 18(36%) while, it was 2(24%), 6(12%) in healthy group. Tumor necrosis factor alpha (TNF-α) was also estimated in serum of all subjects by using ELISA method too. The mean levels of TNF-α in seropositive anti-Toxoplasma IgM and IgG of RA+MTX patients were 3.781 pg/ml ± 0.571) and (36.98 pg/ml ± 0.58), in RA patients (25.404 pg/ml ± 1.748) and (40.12 pg/ml ± 1.7) while, they were (5.04 pg/ml ± 0.643) and (10.7 pg/ml ± 1.7) in healthy group. The results showed significant difference (P<0.05) was found between treated and untreated patients.

أجريت هذه الدراسة لتقييم معدل انتشار داء المقوسات الكونيدية في294 مريض بمرض التهاب المفاصل الرثياني المعالجين بالميثوتركسيت, و50 مريض بمرض المقاصل التهاب الرثياني غير معالجين و50 عينة كعينات سيطرة. تم جمع العينات وتحديد وجود الأجسام المضادة لطفيلي المقوسات الكونيدية من النوع (ج) و (م) باستخدام تقنية الاليزا. كذلك تم قياس عامل التنخر الورمي بتقنية الاليزا أيضا. وكانت الأجسام المضادة لطفيلي المقوسات الكونيدية من النوع (ج) و (م) في مجموعة التهاب المفاصل الرثياني المعالجين بالميثوتركسيت 60(20.408%)و 98(33.33%), ولمرضى التهاب المفاصل الرثياني غير المعالجين 4(8%)و 18(63%) أما في مجموعة السيطرة 2(24%)و6(12%). وكان معدل مستوى عامل التنخر الورمي في الأشخاص المصابين بطفيلي المقوسات الكونيدية من النوع (ج) و (م) في مجموعة التهاب المفاصل الرثياني المعالجين بالميثوتركسيت ( (3.871pg/ml±0.571و (36.98pg/ml±0.58), ولمرضى التهاب المفاصل الرثياني غير المعالجين (25.404 pg/ml ±1.748), (40.12 pg/ml ± 1.7) وفي مجموعة السيطرة 0.643) (5.04 pg/ml ±, و (10.7 pg/ml ± 1.7). أوضحت النتائج وجود فروق معنوية (P<0.05 ) بين المرضى المعالجين وغير المعالجين .


Article
THE PROTECTIVE EFFECTS OF FELODIPINE ON METHOTREXATE-INDUCED HEPATIC TOXICITY IN RABBITS

Authors: Noor H. Swayeh نور حسون صويح --- Ahmed R. Abu-Raghif احمد رحمه ابو رغيف --- Ban J. Qasim بان جمعة قاسم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 166-173
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Methotrexate is folic acid antagonist, used in the therapy of various types of diseases. Oxidative stress and inflammation have the major role in methotrexate toxicity.Objective:To study the protective effects of felodipine against methotrexate-induced hepatotoxicity in rabbits.Methods:Twenty four rabbits divided randomly into three groups. Group I was left without treatment, group II received a dose of 20 mg/kg methotrexate (MTX) intraperitoneally from 3rd day for three successive days and group III received a dose of 0.5 mg/kg felodipine orally for 7 days in addition to MTX therapy similar to group (2). On 8th day the following parameters (liver enzymes, liver tissue homogenate of glutathione, malondialdehyde and tumor necrosis factor-α) were monitored. As well as assessment of histological changes on liver tissue sections after scarification.Results:Administration of felodipine significantly decreased the elevated levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), malondialdehyde and tumor necrosis factor-alpha. It also elevated glutathione levels significantly, with improvement of histological features related to MTX exposure in group III compared with group II.Conclusion: Felodipine can protect hepatic tissue against MTX-induced hepatotoxicity.Keywords: Hepatotoxicity, Methotrexate, Felodipine, Glutathione, Malondialdehyde, Tumor Necrosis Factor-alpha.


Article
Effect of orthodontic tooth movement on salivary levels of Interleukin-1beta, Tumor Necrosis Factor-alpha, and Creactive protein

Authors: Noor Saadi --- Nidhal H. Ghaib نضال حسين غايب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 4 Pages: 120-125
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Orthodontic force is considered to stimulate cells in the periodontium to release many mediators suchas cytokines which play a responsible role for periodontal and alveolar bone remodeling, bone resorption and newbone deposition. Aim of this study was carried out to estimate changes of the (interleukin-one beta, tumor necrosisfactor – alpha and C-reactive protein) levels in unstimulated whole saliva during the leveling stage of orthodontictooth movement.Materials and methods: The sample consisted of thirty adult patients (12 males and 18 females) with ages ranges (19-23) years. Each sample had Class I and Class II malocclusion dental classification and required bilateral extraction oftheir maxillary first premolars, underwent a session of professional oral hygiene and received oral hygiene instructionsbefore and during the period of study, one month later fixed orthodontic appliance were placed in the upper archby using 0.014 nickel titanium arch wire. The unstimulated whole saliva was taken from each sample immediatelybefore placement of the appliance (baseline), and at (after1hour, after one week and after two week) followingplacement of the fixed orthodontic appliance. In addition the plaque index and gingival index were recordedduring the interval periods of this study to assess oral cleanliness. The interleukin – one beta and tumor necrosis factoralphawere determined by enzyme linked immunosorbent assay, while the C-reactive protein was determined bylatex agglutination.Results: The results of the present study found the mean value of both salivary (interleukin-one beta and tumornecrosis factor -alpha) were highest at (after1hour) followed by at (after one week) then at (after two week) thanthe baseline with highly statistical significant differences (P< 0.01) among males, females and total samples, inaddition there were no statistical significant differences between males and females (P>0.05). While the mean valuesof C-reactive protein were higher at (after 1hour) only with highly significant differences (P< 0.01) among femalesand total samples while only significant difference (P<0.05) for males, in addition there were no statistical significantdifferences between males and females. Regarding the correlation between salivary (interleukin – one beta andtumor necrosis factor -alpha), there were positive correlation between them at all periods of study. Moreover therewere positive correlation between salivary (interleukin – one beta and tumor necrosis factor -alpha) and salivary Creactiveprotein. On the other hand there were no association between the salivary (interleukin – one beta andtumor necrosis factor -alpha and C-reactive protein) and clinical parameter (plaque index and gingival index).Conclusion: From this clinical study we conclude that orthodontic force induces increasing the levels of (interleukin –one beta, tumor necrosis factor -alpha, C-reactive protein) in unstimulated whole saliva during orthodontic toothmovement.


Article
Tumor Necrosis Factor Alpha Synergetic with Insulin Resistance Potentially Contributes to the Development of Polycystic Ovary Syndrome.

Authors: Noor-Alhuda M. Al-Assadi --- Suhaila* f. AL-Shaikh --- Suhayr A. Al-Qaysi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 4 Pages: 732 -737
Publisher: Babylon University جامعة بابل

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Abstract

Introduction: polycystic ovary syndrome (PCOS) is one from record common endocrine disorders, which is complicated in the multi system disease and its etiology is silent not clearly understood. The environment factors and the genetic factors production a crucial role in the pathogenesis of PCOS. The obesity is android in type with greater waist to hip ratio and obese in posterior abdominal wall and mesentery. Due to that (50%) of women with PCOS are fat, additional the possibility of insulin resistance is the development of type 2 diabetes. Aims of the study to estimate the role of TNF-α and insulin resistance in pathogenesis of PCOs and identify the relationship of obesity with IR and TNF-α in PCOs. IR and TNF-α concentration was assessed by competitive ELISA method. Significant of differences were detected between PCOS patients and controls. Each the PCOS had raised up BMI, WHR, fasting insulin, homeostatic model assessment (HOMA) score, and serum TNF-α when matched with controls (p<0.05). The conducted examination indicated the contribution of TNF-α in path mechanism of PCOS being the basis of increase body weight which lead to development of insulin resistance.


Article
Levels of Cytokines Profile in Polycystic Ovary Syndrome

Author: Shafaq H. Hussein Al‑Musawy, Ihsan E. Al‑Saimary, Maysoon Sherif Flaifil
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 124-128
Publisher: Babylon University جامعة بابل

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Abstract

Background: Polycystic ovary syndrome (PCOS) is one of the commonest endocrine disorder of female at reproductive age and it's prevalencein general population as 20%–33%. Objective: The aim of this study was to measure the level of interleukin (IL) 18, IL 6, and tumor necrosisfactor (TNF) in polycystic ovary women and compare their levels with apparently healthy control group. Materials and Methods: A case‑controlstudy was carried out in Basra. In this study, blood samples from 73 women with PCOS and 73 healthy control women were collected fromoutpatients and private gynecological clinics and primary health care centers from different area of Basra during August 2016–March 2017for estimation of their serum levels of IL6, IL18, and TNF alpha by using enzyme‑linked immunosorbent assay technique. Results: It is foundthat serum levels of IL6, IL18, and TNF alpha were elevated in PCOS women, and we conclude that serum levels of IL6, IL18, and TNFalpha are highly statistically significance in PCOS women than in healthy control group. Conclusion: Levels of IL6, IL18, and TNF alphawere highly statistically significant in PCOS comparing to normal women, and these high levels were related to PCOS independent on thepresence of obesity or hyperandrogenism.


Article
Evaluate Of Tumor Necrosis Factor - Α and Interleukin-10 of a Sample of Pregnant Women in Diyala Province During The Three Stages of Pregnancy
تقييم عامل التنخر الورمي الفا والبين الابيضاضي العاشر لعينة من النساء الحوامل في محافظة ديالى خلال مراحل الحمل الثلاثة

Authors: Mohammed A. Saleh محمد عبد الدايم صالح --- Inas S. Sabih أيناس سعد صبيح
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2017 Volume: 13 Issue: 2 - part 3 Pages: 208-221
Publisher: Diyala University جامعة ديالى

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Abstract

This study was aimed to evaluate the immunological role of the tumor necrosis factor - alpha and Interleukin- 10 in some sample of pregnant women during three stages in Diyala province. A total of 90 blood samples were collected from pregnant women, also 30 blood samples were collected from non-pregnant apparently healthy women as a control group during the period (1/2015/10 to 1/2/2016) from the Al-Batool Teaching hospital for Gynecology and Children. The results showed that the mean age for pregnant group was 25.94±6.12 year, While for control group was 27.03±6.34 year a additionally the pregnant women group. Pregnant women's group was divided into three stages (each stage included 30 blood sample). On the other hand the results of this study showed a significant decrease (p≤0.001) in the TNF-α level in pregnant women compared to the control group with highly significant differences (p≤ 0.001) through three periods of pregnancy. Also, results revealed a significant increase (p≤ 0.001) at the level of IL-10 in pregnant women compared to the control group with highly significant differences (p≤0.001) in the level of IL-10 during the three stages of pregnancy. Shows through a these results that interleukin - 10 was his a positive role in maintaining pregnancy, while notes of tumor necrosis factor alpha negative role in the process of pregnancy.

هدفت هذه الدراسة الى تقييم الدور المناعي لعامل التنخر الورمي الفا والبين الابيضاضي العاشر لعينة من النساء الحوامل خلال مراحل الحمل الثلاثة في محافظة ديالى . جمعت 90 عينة دم من النساء الحوامل و30 نموذج دم من النساء غير الحوامل الاصحاء ظاهرين (كمجموعة سيطرة ) خلال الفترة الممتدة (1 /2015/10 الى2016/2 /1) من المراجعات مستشفى بتول التعليمي للنسائية والاطفال. أظهرت نتائج الدراسة بان معدل العمري للمجموعة النساء الحوامل كانت (25.94 ± 6.12 ) سنة ، بينما كان معدل مجموعة السيطرة 27.03)± 6.34 ) سنة . بالاضافة الى ذلك قسمت مجموعة النساء الحوامل الى ثلاث مراحل كل مرحلة من مراحل الحمل تتضمن 30 نموذج دم) . من ناحية اخرى اوضحت نتائج هذه الدراسة انخفاض معنوي (p≤0.001) في مستوى تركيز (TNF-α) في النساء الحوامل مقارنة بمجموعة السيطرة ، بينما أظهرت الدراسة فرق معنوي عالي عند مستوى الدلالة (p≤ 0.001) خلال فترات الحمل الثلاثة في مستوى (TNF-α)، وأظهرت الدراسة ارتفاع معنوي (p≤ 0.001) في مستوى تركيز (IL-10) في النساء الحوامل مقارنة بمجموعة السيطرة ، في حين اظهرت الدراسة ايضا فرق معنوي عالي p≤0.001)) في مستوى تركيز (IL-10) خلال المراحل الحمل الثلاثة ، ويوضح من خلال هذه النتائج ان الانترلوكين - 10 له دور ايجابي في المحافظة على عملية الحمل بينما يلاحظ للعامل التنخر الورمي الفا دور سلبي في عملية الحمل.


Article
Immunological study among Tuberculosis patients in Baghdad
دراسة مناعية بين مرضى التدرن في بغداد.

Authors: May. Y. Abdul-Hussein مي يحيى عبد الحسي --- Oruba Kh. Abbas عروبة خالد عباس --- Ahmed A. Mankh احمد اسمر منخي
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2015 Volume: 28 Issue: 4 Pages: 202-206
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Mycobacterium tuberculosis (Mtb) infection may cause overt disease or remain latent. Interferon gamma release assays (IGRAs) detect Mtb infection, both latent infection and infection manifesting as overt disease, by measuring whole blood interferon gamma (IFN-Ɣ) responses to Mtb antigens such as ESAT-6, CFP-10 and TB7.7. Biomarkers are crucial to the development of new diagnostic tools for tuberculosis diagnosis and could be instrumental in reducing morbidity and mortality and curtailing spread of tuberculosis. In this study, cytokines such as Tumor necrosis factor –alpha (TNF-α) and Interleukine-10 (IL-10) in addition to IFN- Ɣ were assessed as biomarkers of tuberculosis infection and reactivation; tuberculosis disease and tuberculosis cure.Objective: This study was designed to evaluate the immune status of patients with respect to Mycobacterium tuberculosis. A prospective study was conducted to obtain more clarification about the impact of causative agent . The study included immunological diagnosis by Quantiferon –TB Gold-Test (QFT) and estimation the serum levels of IFN-ɣ, TNF-α and IL-10 in TB patients and control.Methods: The present study included fifty TB patients attending the National Reference Laboratory of Tuberculosis /Baghdad during the period April to July 2014 were recruited for this study. For the purpose of comparison, 50 control samples (respiratory disease and healthy ) matched by age and gender were also included. The patients and control groups were examined for the presence of TB by Quantiferon TB Gold test.Results: Forty five samples of TB patients with significance difference (p≤0.01) were positively detected by Quantiferon-TB Gold Test. The present study revealed significant difference in IFN-ɣ, TNF-α and IL-10 levels between TB patients and control groups.Conclusion: High levels of Interferon-gamma, Tumor necrosis factor-alpha and Interleukine-10 in tuberculosis patients serum indicate an important role of this cytokines in the pathogenesis of tuberculosis, so they could be considered as a good biomarkers for diagnosis and considered as a target for future therapy.

الخلفية: ان الاصابة ببكتريا التدرن ممكن تسبب مرض ظاهر او تبقى الاصابة كامنة. حساب الانترفيرون كاما المتحرر يستخدم لتشخيص الاصابة سواء كانت كامنة ام ظاهرة من خلال قياس استجابة الانترفيرون المتحرر لمستضدات البكتريا مثل ESAT-6 ,CFP-10,TB 7.7 . المؤشرات الحيوية ذات اهمية لتطوير ادوات تشخيصية لتشخيص مرض السل ويمكن ان تكون مفيدة للحد من الوفيات وللحد من انتشار المرض. في هذه الدراسة تم حساب حركيات خلوية (سايتوكينات ) مثلTumor necrosis factor- alpha,Interleukine-10 بالاضافة الى Interferon-gamma كمؤشرات حيوية للاصابة ولتنشيط المرض او الشفاء منه. الهدف: تم تصميم هذه الدراسة لتقييم الحالة المناعية عند المرضى فيما يتعلق ببكتريا التدرن وقد اجريت هذه الدراسة لتسليط الضوء اكثر حول تأثير البكتريا المسببة لهذا المرض. تضمنت الدراسة تشخيص مناعي باستخدام اختبار Quantiferon-TB Gold Test وتقييم المستوى المصلي للحركيات الخلوية (IL-10,TNF-α,IFN-ɣ) في مرضى التدرن ومجاميع السيطرة(مرضى الجهاز التنفسي و الاصحاء) .المواد و طرائق العمل : أجريت الدراسه على50 مصاب بمرض التدرن ممن كانوا يراجعون المختبر المرجعي للامراض الصدرية والتنفسية في بغداد , ولغرض المقارنه اعتمد 50 شخصا من السيطرة (مرضى الجهاز التنفسي و الاصحاء) متوافقين بالعمر و الجنس مع المرضى. كل من المرضى و مجاميع السيطرة تم التحري عن المرض باستخدام اختبار Quantiferon-TB Gold test.النتائج : اظهرت النتائج ان 45عينة من عينات المصابين كانت موجبة باستخدام اختبار . QFTكما اظهرت النتائج فروق معنوية في تراكيز IFN-Ɣ, TNF-α , IL-10 بين المرضى و مجاميع السيطرة

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