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Article
9- ISOLATION OF 9-STREPTOCOCCUS AGALACTIAE FROM WOMEN WITH UTERINE TUMORS

Authors: Kudus E Omaro --- Haifa ALShaheen --- Hadeel T AL-Hadithi
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2009 Volume: 15 Issue: 1 Pages: 53-60
Publisher: Basrah University جامعة البصرة

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Abstract

Streptococcus agalactiae was isolated from eight out of 42 cases of uterine tumors andendometrial hyperplasia from inpatients underwent total abdominal hysterectomy in BasrahMaternity and Child Hospital, due to irregular vaginal bleeding not responding to medical andhormonal treatment. Six isolates were from uterine tumors, and two from endometrialhyperplasia. Isolates were identified by being Gram positive, negative for catalase, givingpositive CAMP test, ability to grow in 6.5% NaCl but not 40% Bile salts and resistance toBacitracin.In vitro studies were conducted to investigate virulence factors of isolated S. agalactiae bytesting their ability to produce haemolysin, yellow pigmentation, capsule, resistance totetracycline which is indicative for the existence of sialic acid (antiphagocytosis material).Measurement of the content of sialic acid and lipotechoic acid revealed differences betweenisolates.All isolates, were able to adhere to plastic surfaces and formation of biofilms in both neutral(pH=7) and acid (pH=4.5) media. The present study has recorded for the first time, resistance offour isolates of S. agalactiae (50%) to Vancomycin. This finding is worrisome from the clinicalpoint of view, as these isolates may become a potential source for transmission of Vancomycinresistance to other bacteria.


Article
Biochemical Study of Ribonuclease in Serum and Tissues of Patients with Uterine Tumors
دراسة كيموحيوية لأنزيم الرايبونيوكليز RNase في مصل دم وأنسجة المصابات بأورام الرحم

Authors: Saba Z. Al-Abachi صبا زكي العباجي --- Duaa H. Omar دعاء حسين عمر
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2013 Volume: 24 Issue: 5A Pages: 64-80
Publisher: Mosul University جامعة الموصل

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Abstract

The activity of alkaline and acidic ribonuclease (RNase) was measured in serum and tissues of women with benign and malignant uterine tumors, compared to women with a curettage as a control group. The results indicated that there is a significant increase in alkaline and acidic RNase activity in sera and tissues of patients with uterine tumors when compared with the control group. The research also, includes the isolation and purification of the alkaline RNase from the sera of normal women. It has been found that the specific activity of enzyme in proteinous precipitate, after dialysis and gel filtration, was increased and the enzyme shows two peaks. In addition, it has been found that the enzyme has approximate molecular weight of (28774±1000) dalton. The results also showed that the optimum conditions of the RNase enzyme are: (50) µg/ml of enzyme concentration with reaction (15) minutes at (pH= 8.2) and at (37˚C), while the substrate concentration was about (0.125) mmol/L. When the Lineweaver-Burk plot was used, the value of Michaelis-Mentens constants (Km) is (0.06 mmol). When the duration of storage was studied, the activity of the enzyme is not affected during two months, after that the activity was decreased gradually.

تم قياس فعالية أنزيم الرايبونيوكليز RNase القاعدي والحامضي في مصل ونسيج النساء المصابات بأورام الرحم الحميدة والخبيثة مقارنة مع مصل ونسيج نساء تعرضن لعملية قشط جدار الرحم بوصفها مجموعة سيطرة. لوحظ وجود زيادة معنوية في فعالية الأنزيم القاعدي والحامضي لدى النساء المصابات بأورام الرحم مقارنة مع مجموعة السيطرة. تضمن البحث أيضا عزل وتنقية أنزيم RNase القاعدي من مصل الدم لإمرأة سليمة، وأشارت النتائج الى وجود زيادة في الفعالية النوعية للأنزيم في الراسب البروتيني بعد الترسيب بكبريتات الامونيوم المشبعة وبعد الترشيح باستخدام تقنية الترشيح الهلامي ظهر للأنزيم حزمتين بروتينيتين. تم تقدير الوزن الجزيئي التقريبي للأنزيم إذ بلغ (1000±28774) دالتون. وتم ايضا دراسة تأثير بعض العوامل على فعالية أنزيم RNase القاعدي وتبين أن الظروف المثلى للأنزيم كانت عند تركيز (50) مايكروغرام/مل من الأنزيم وبزمن تفاعل (15) دقيقة عند (pH=8.2) ودرجة حرارة (37˚C)، عندما يكون تركيز مادة الاساس (0.125) ملي مول/لتر وباستخدام رسم لاينويفر- برك وجد أن قيمة ثابت مكيلس هي (0.06) ملي مول. وعند دراسة تأثير مدة التخزين على فعالية الأنزيم وجد أن الفعالية تبدأ بالانخفاض بعد شهرين من التحضير.

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