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Article
Vascular Endothelial Growth Factor as Predictive Marker for Hypertension in Iraqi Adults Patients

Authors: Huda Jaber Waheed --- Huda Abdul Ridha Hadi --- Mostafa Abdulfatah Shafek
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 2 Pages: 56-59
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Vascular endothelial growth factor (VEGF) is considered the most potent angiogenic growth factor for the stimulation of collateral vessel growth in peripheral and myocardial ischemia and other coronary heart disease. Aim: The aim of the present study was first to assess the levels of VEGF in adult subjects with hypertension, second to find any correlation between VEGF and other studied biochemical markers. Patients & Methods: Thirty four adults with hypertension (age mean 34.55 ± 3.79 year) from outpatients of Al-yarmook Teaching Hospitals in Baghdad were selected randomly from March to April 2015. For comparison, (30) normotensive subjects which were matching to patients in age and BMI. VEGF levels were measured. Results: Biochemical parameters include FBG, HDL, and cholesterol, TG, HbA1c and PCV. Anthropometric measurements were also recorded. VEGF significantly higher (p ˂ 0.05) in patients group when compared to healthy group, as well as, significant elevation also shown in FPS, HDL and HbA1c, while TG, cholesterol, Creatinine and PCV not affected. Conclusion: VEGF can be used as diagnostic marker for the hypertension in adult patients.

Keywords

Hypertension --- VEGF


Article
Vascular endothelial growth factor gene expression and polymorphism C936T in Iraqi patients with ovarian carcinoma

Author: Maisaa G. Jumaa1 , Khalid K. Tobal2 , Mohammed Ghanim3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 3 Pages: 137-148
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study aimed to shed light on the association between the angiogenic factor VEGF gene expression and its genetic polymorphism VEGF C936T in the angiogenesis of ovarian cancer. A total of 44 Paraffin-embedded tissue blocks from patients with different stages of newly diagnosed ovarian cancer were provided by certain Iraqi hospitals as well as 14 samples of patients with benign ovarian tumors tissues as a control group were used in this study. The results detected that VEGF mRNA in ovarian cancer samples was statistically significant compared to benign tumors(p value=0.039<0.05). The samples were divided into high and low mRNA-expression depending on the mean value of VEGF gene expression in benign tumors which used as a cutoff, the results showed that high statistical significant differences (P value = 0.0023<0.01) between high mRNA-expressing samples 30(76.9%), and low mRNA-expressing samples 9(23.07%). Statistical no significant differences were detected in compare with other histopathology tumor types. In correlation with stages, statistically significant difference was found between 31 (79.4%) of samples with stage I which showed the highest level of expression(P=0.0210<0.05). The result of VEGF C936T polymorphism showed that out of 42 ovarian cancer patients 24(57.14%) were homozygous for the C/C genotype, 11(26.2%) were heterozygous C/T and 7(16.66%) were homozygous T/T. High significant prevalence of the VEGF 936C allele was detected in both ovarian cancer patients (P value 0.0019 <0.01) and benign ovarian tumors (P value 0.0026 <0.01) as compared with VEGF 936T allele. The study showed that the average of VEGF gene expression in ovarian cancer patients carrying the +936CT+TT genotypes (1.815116, 2.298769) was significantly lower than that in ovarian cancer patients with the VEGF+936CC genotype (4.395884). Same results were obtained from patients with benign ovarian tumors. In conclusion the present study investigated that the presence of the VEGF+936 T allele is associated with a decreased risk of ovarian cancer since the C936T polymorphism has been reported to be associated with lower VEGF plasma levels.


Article
Evaluation of Vascular Endothelial Growth Factor-A (VEGF-A) in Iraqi patients with urinary bladder cancer
قياس عامل النمو الوعائي البطاني في المرضى العراقيين المصابون بسرطان المثانة

Authors: Asaad A. Al-Janabi --- Arshad N. Al-Dujaily --- Hanan D. Abbas
Journal: kufa Journal for Nursing sciences مجلة الكوفة لعلوم التمريض ISSN: 22234055 Year: 2015 Volume: 5 Issue: 1 Pages: 42-48
Publisher: University of Kufa جامعة الكوفة

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Abstract

Objective: The aim of this work is to study the alterations in the vascular endothelial growth factor (VEGF-A), and its role in pathogenicity of bladder cancer in serum of men who are initially diagnosed with this disease.Methods: The current study was applied in pathology department of British United Kingdom kings college hospital during April 2012 to August 2013 by using Enzyme Linked Immuno Assay (ELISA) on serum taken from 42 men infected with bladder cancer , and other 38 benign considered as a control group to detect vascular endothelial growth factor type A (VEGF-A).Data analysis: SPSS computerizing system has been used for statistically analysis of data.Results: The results revealed a highly significant (P<0.05) level of VEGF-A in serum of patients in comparison with the control group.Conclusion:It was concluded from the results of the present study that VEGF-A was good prognostic biomarker for urinary bladder cancer in men ,and these results significantly correlate with the early stages of disease. Recommendation : It may be recommended that using VEGF-A to predict and diagnosis the bladder cancer.

هدف الدراسة:إيجاد العلاقة بين مرض سرطان المثانة وعامل النمو الوعائي البطاني ودوره في تشخيص هذا المرض.منهجية البحث:أجريت الدراسة الحالية في بريطانيا في قسم الباثولوجي في المستشفى الملكي للفترة من نيسان 2012 ولغاية آب 2013 على عينات الدم المأخوذة من المرضى الذكور العراقيين المصابون بمرض سرطان المثانة البالغ عددهم 42 مريض وآخرين أصحاء عددهم 38 استخدموا كمجموعة سيطرة.الوسائل الإحصائية:تم استخدام نظام (SPSS) الكومبيوتري لتحليل النتائج إحصائيا. النتائج :أظهرت نتائج الدراسة الحالية زيادة معنوية عالية(p < 0.05) في مستوى عامل النمو الوعائي البطاني لدى المرضى مقارنة بمجموعة السيطرة.الاستنتاجات: من خلال الدراسة الحالية تم الاستنتاج بالإمكان اعتماد عامل النمو الوعائي البطاني كعامل تشخيص لمرض سرطان المثانة لاسيما في المراحل المبكرة من المرض.التوصيات :1- قد يوصى من خلال الدراسة الحالية إجراء دراسة جينية متقدمة لمعرفة العلاقة بين عامل النمو الوعائي البطاني والتغييرات الجينية لسرطان المثانة. 2- إجراء قياس العامل المذكور أعلاه للمرضى المصابون بسرطان المثانة في بداية ظهور المرض للتنبؤ بحدوثه.

Keywords

VEGF-A --- bladder cancer


Article
Immunohistochemical expression of VEGF in relation to VEGFR and CD34 in NHL using digital image analysis system

Authors: Bassam Mohammad Hameed --- Hind Shaker Aboob --- Raad Jaber Musa
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2016 Volume: 5 Issue: 1 Pages: 19-31
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Lymphoma growth and progression appear to be promoted by at least two distinctangiogenic mechanisms: autocrine stimulation of tumor cells via expression of Vascular EndothelialGrowth Factor(VEGF) and Vascular Endothelial Growth Factor Receptor(VEGFR) by lymphomacells, and paracrine influences of the proangiogenic tumor microenvironment on local tumorvascularity.Objectives: To assess autocrine effect of VEGF, by studying the correlation of VEGF expressionwith its receptor VEGFR expression in NHL. And to assess paracrine effect of VEGF, by studying thecorrelation of VEGF expression with CD34 expressed on endothelial cells in Non-HodgkinLymphoma (NHL).Materials and Methods: A cross sectional study was designed. A total of 66 bone marrow tissuesamples were included in the study, all diagnosed as having NHL according to working formulation.From each block, 3 sections were taken, and were immunohistochemically stained for CD-34, VEGFand VEGFR. Scoring of Immunohistochemical staining was performed using specialized automatedcellular image analysis system, Digimizer software, version 3.7.0.Results: VEGF Immunohistochemical digital parameters named digital labeling index (DLI) wassignificantly correlated with the followings; VEGFR (DLI) [P =0.042, r =0.324], CD34 stained area(A) [P=0.037, r =0.556]. Also VEGFR (DLI) was significantly correlated with CD34 (A).Conclusion: Autocrine and paracrine effect of VEGF is evident in NHL, as there is positivecorrelation between VEGF expression and VEGFR expression, and as tumor vascularity increase withthe increase in VEGF expression.

Keywords

NHL --- VEGF --- VEGFR


Article
mRNA in situ hybridization analysis of VEGF in chronic lymphocytic leukemia

Authors: Haythem A. MJ Al-Rubaie --- Ban A. Abdul Majeed بان عباس عبد المجيد --- Abdulkareem M. Jaafar
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2011 Volume: 53 Issue: 2 Pages: 186-189
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Several factors render chronic lymphocytic leukemia (CLL) an interesting subject for study by researchers. These include marked progress in understanding the molecular biology of normal and neoplastic lymphocytes and recent advances in molecular genetics techniques. Among molecular markers, vascular endothelial growth factor (VEGF), have been widely studied.Objective: The aim of the study is to evaluate the role of VEGF in the pathogenesis of CLL and its role in disease progression.
Patients, materials and methods: A retrospective cross-sectional study was done on 60 patients with chronic lymphocytic leukemia (45 males & 15 females) compared with 20 controls (anemic patients), all recruited at the Medical City Hospital/ Teaching Laboratories/ Baghdad from January 2004 to December 2007. The bone marrow biopsy of each was re-examined histologically. In situ hybridization was performed utilizing biotin labeled VEGF cDNA probes.
Results: The frequency of VEGF positivity was 95% (57 of 60 cases). A statistically significant inverse correlation was found between increasing VEGF scores and lower packed cell volume level and lower platelet count. A statistically significant association was found between advanced modified Rai stage and patients with high score. In addition, there was a statistically significant positive correlation between increasing scores of VEGF and advancing clinical stage.
Conclusions: The score of VEGF is high among patients with advanced clinical stage of the disease.


Article
Immunohistochemical expression of D2-40, VEGF and PCNA as biological markers of lymphangiogenesis, angiogenesis and proliferation in pleomorphic adenoma of salivary gland origin

Authors: Hiba Jasim Rassol هبة جاسم رسول --- Kadhim Al-Soudani كاظم السوداني
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: special issue 1 Pages: 53-58
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Pleomorphic adenoma is the most common benign salivary gland tumor and shows a pronouncedmorphological complexity and diversity; for this The immunoprofiles and clinical course of PA differed according tocellular differentiation. Therefore, it is important to assess potential biomarkers in diagnostic and therapeutic trials. Thisstudy evaluates the immunohistochemical expression of D2-40, VEGF and PCNA as markers of lymphangiogenesis,angiogenesis and proliferation of PA and their correlation with clinicpathological parameters and with each other.Materials and Methods: Twenty five formalin – fixed, paraffin – embedded tissue blocks were included in this study.After histopathological reassessment of haematoxylin & eosin stained sections for each block, animmunohistochemical staining was performed using anti D2-40, anti VEGF and anti PCNA monoclonal antibodies.Results: Positive immunohistochemical of D2-40, VEGF and PCNA was found in (100%), (92%) and (88%) of the casesrespectively. No statistically significant correlation neither among the markers immunoexpression nor with theclinicopathological finding, except a statistical significant correlation was found between (D2-40andVEGF)expression with the histopathological presentation of the cases.Conclusion: D2-40, VEGF and PCNA immunoexpression showed significant correlation with respect to thehistopathological presentation of the cases. While no significant correlation seen regarding the expression ofaforementioned markers with each other, suggests that each marker might affect on tumor behavior independently

Keywords

Pleomorphic adenoma --- D2-40 --- VEGF --- PCNA


Article
Histological evaluation of local application of collagen I and /or vascular endothelial growth factor (An experimental study in rats)

Authors: Rafal Rashid رفل رشيد --- Athraa Y. Al-Hijazi عذراء يحيى الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 3 Pages: 79-84
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The study was designed to evaluate the effect of local application of exogenous VEGF/collagen Iseparately and as a combination in socket healing. Sixty male Albino Wistar rats were subjected for a surgical toothextraction of upper 1st molar of both sides (right side was considered as experimental site, while left be the controlone, treated with 1μL of normal saline). The rats were scarified at 3, 7, 14, 28 days post extraction. Socket healing washistologically examined with immunohistochemistochemical localization of ALP&FGF2.Materials and Method: Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1stmolarof both sides (right side was considered as experimental site, while left be the control one). The animals were dividedinto following groups according to the applicable of biomaterials.A. Control group the tooth socket treated with 1μL of normal salineB. Experimental group includes• Group I contains (20) rats, the tooth socket treated with 1μL of VEGF• Group II contains (20) rats the tooth socket treated with 1μL of collagen type I• Group III contains (20) rats, the tooth socket treated with 1μL of a combination of VEGF and collagen I.Results: At 28 days all groups show re-epithelization but in different thickness, and with newly bone apposition andwith different maturity. For positive cells expressed ALP, VEGF group records a high mean values at 3, 14, 28 daysperiods and with high differences in comparison to other groups while control group reports a high mean value at 7days. For positive cells expressed FGF2. Control group illustrates a high record for the mean of positive cellsexpressed FGF2 at 3,7days periods and with high differences in comparison to other groups, while combinationgroup reports a high mean value at 14 days .Conclusion: Results, high lighted on the effect of local application of VEGF in extracted tooth socket that facilitateepithelization, while combination of (collagen and VEGF) shows a high mineralization zone


Article
Assessment of Periodontal Health Status and Serum Level of Vascular Endothelial Growth Factor in Women with Breast Cancer

Authors: Areej A. Abdul-Hafidh اريج عبد الفتاح --- Maha Sh. Mahmood مها شكري محمود
Journal: Tikrit Journal for Dental Sciences مجلة تكريت لعلوم طب الاسنان ISSN: 20731213 Year: 2017 Volume: 5 Issue: 2 Pages: 11-18
Publisher: Tikrit University جامعة تكريت

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Abstract

Periodontal diseases are inflammatory diseases that affect theperiodontium which lead to alveolar bone and attachment loss.Breast cancer is the most common cancer in women worldwide.Expression of (VEGF) is elevated in both diseases and it is themarker of angiogenesis related to cancer development. 1. Todetermine the periodontal health status and the serum levels of(VEGF) in women with breast cancer in comparison to womenwithout breast cancer. 2. To correlate between serum levels ofVEGF with clinical periodontal parameters (PLI, GI, BOP, PPD,and CAL) in women with breast cancer in comparison to womenwithout breast cancer. Eighty women with age ranged from (30-60) years old, 40 women with breast cancer and another 40 womenwithout. Periodontal health status were taken for all participantsand then they were subdivided into four groups: the (BC +CP)group which included participants with breast cancer and chronicperiodontitis (n=20), the (BC+ G) group included participants withbreast cancer and gingivitis (n=20), the (CP) group which includedparticipants with chronic periodontitis only (n=20) and the (G)group which included participants with gingivitis only (n=20).Other than breast cancer, all subjects were systemically healthyfemales. Periodontal health status was determined by clinicalperiodontal examination of (PLI, GI, BOP, PPD and CAL). Bloodsamples were collected from each participant, serum levels ofVEGF were determined by enzyme -linked immune-sorbent assay(ELISA). The results of this study showed that the median valuesof PLI and GI were slightly increased in BC+G group incomparison to other groups with non-significant differences, thepercentages of BOP showed highly significant differences amongthe groups (P <0.01). The median values of PPD showed slightincrease in BC+CP group than CP group with non-significantdifferences. Median values of CAL were increased in CP groupthan BC+CP group with non-significant differences. VEGF serumlevels were elevated in BC+CP group than other groups with nonsignificantdifferences. Also by using Spearman’s rank CorrelationCoefficient, serum levels of VEGF were correlated positively withall clinical periodontal parameters. VEGF serum level could beassociated with progression of both periodontal diseases and breastcancer.


Article
•Pathological Study Of Renal Cell Carcinoma By Expression Of Vascular Endothelial Growth Factor (VEGF) And P53 (Immunohistochemical approach…

Authors: Alaa. S. Jumaa --- As' ad A. Al-Janabi --- Zainab A.AL-Ali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2011 Volume: 4 no 2 Issue: 10 Pages: 1128-1132
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Renal cell carcinomas (RCC) represent about 1-3% of all visceral cancers. There are 30,000 new cases per year and 12,000 deaths from the disease in USA (1).Aim: To estimate the rate of VEGF & P53 immunohistochemical overexpression in RCC in relation to histological types of tumor.Methods: Fomalin fixed, paraffin-embedded blocks from 48 patients (28 male and 20 female) with RCC included in this study. A group of 12 patients with non specific inflammation –pyelonephritis were included as a control group. LSAB+ (Labeled Strept-Avidin Biotin) method was employed for immunohistochemical detection of VEGF & P53.Results: In assessment of the correlation of VEGF overexpression in RCC with histological types, there is correlation between the intensity of VEGF expression & classical type as (R= 0.618) while in other histological types no correlation & no significant difference (p>0.05) between them. In assessment of the correlation of P53 overexpression in RCC there is a negative correlation (R=-0.843) in classical, in papillary (R=-0.674) & no significant difference between them (p>0.05).Conclusion: these finding provide further evidence for the role of VEGF & P53 in the carcinogenesis of RCC. However both markers not well correlated with histological types of the tumor.


Article
VEGF EXPRESSION IN TROPHOBLASTIC TISSUE OF WOMEN WITH SPONTANEOUS MISCARRIAGE AND INFECTED BY TOXOPLASMA GODNII USING INSITU HYBRIDIZATION
التعبير الموقعي لعامل النموVEGF في النسيج المغذي للجنين (التروفوبلاست) لنساء تعرضن للإجهاض الفجائي نتيجة الإصابة بداء المقوسات

Author: Nidhal A. AL-Mohaimen
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2012 Volume: 11 Issue: 2 Pages: 494-502
Publisher: Baghdad University جامعة بغداد

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Abstract

A total of fifty women, aged between (16-42) years, were involved in this study. Enzyme linked immunosorbent assay (ELISA) test was used for the detection of specific IgM (using serum samples) and insitu hybridization method was used for the detection of VEGF m RNA in trophoplastic tissue. Samples were classified into three groups: Group A-patients with spontaneous miscarriage and Toxoplasma godnii positive (n= 20 women), with a mean age of(23.8±1.631);Group B-patients with spontaneous miscarriage and Toxoplasma godnii negative (n=20 women), with a mean age of (25.5± 1.60); Group C- control group, women with induced abortion for medical causes (n=10 women), with a mean age of (26.4± 1.628). The highest percentage of Vascular Endothelial Growth Factor mRNA expression (65.30± 3.587)% was within the induced abortion group, while the lowest expression was within (A) and (B) group (35.45± 2.184)% and (37.65± 2.964)% respectively. These results indicated that VEGF is essential factor in the process of blood vessel formation in the successful pregnancy, and its low level in miscarriage groups indicated its role in the etiology of miscarriage irrespective of toxo status.

تضمنت الدراسة الحالية خمسون إمرأة يعانين من الاجهاض التلقائي وعشرة نساء عندهن إجهاض مُحث لأسباب مرضية. قسمت الدراسة المرضى الى مجاميع على أساس نتائج الفحوص الى:المجموعة الاولى(A) والتي شملت 20 إمرأة تعاني من الاجهاض التلقائي وهي موجبة لمُستضدات طفيلي داء المقوسات. المجموعة الثانية(B) وشملت 20 إمرأة تعاني من الإجهاض التلقائي وهي سالبة لمُستضدات طفيلي داء المقوسات. المجموعة الثالثة(C) (مجموعة السيطرة) وشملت عشرة نساء عندهن إجهاض مُحث لأسباب مرضية. تم جمع الدم الوريدي من النساء لغرض إستخراج المصل وفحص نسبة المضاد المناعي نوع IgM بواسطة فحص ELISA. وفي الوقت نفسه تم جمع نسيج "التروفوبلاست " أثناء عملية الكشط (الكرتاج), وقد أُستعمل التعبير الموقعي للحامض الرايبوزى الناقل (ISH) لعامل النمو(VEGF mRNA). بينت النتائج إن التعبير الموقعي لـVEGF mRNA ظهر بأعلى نسبة في المجموعة(C) (65.30±3.587)% , أما النسب الأقل فهي في المجموعة(B)(37.65±2.964)% و(A),(35.45±2.184)% على التوالي وكان هناك فرق معنوي عالي بين المجموعة (A) والمجموعة (C) (P<0.001). ومن هذه النتائج يظهر أن عامل النموVEGF له دور مهم في عملية الإجهاض التلقائي.

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