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Article
Study of virulence factors and inhibitory effect of salvia on identified gram positive and gram negative isolated from soil

Author: Zahrah Adnan Dikhel
Journal: Misan Journal of Acodemic Studies مجلة ميسان للدراسات الاكاديمية ISSN: 1994697X Year: 2017 Volume: 16 Issue: 32 Pages: 104-116
Publisher: Misan University جامعة ميسان

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Abstract

Soil is considered one of the most suitable environmental for microbial growth . In this study and 130 bacterial isolates were from soil in musian city the first most common ratio was Escherichia .coli 30 (23.07) % and less of occurrence ratio was Citrobacter freundii 1(0.76) % also study some virulence factors like heamolysin the high ratio was Enterobacter spp 10(4)% and low ratio in Citrobacter freundii 1(1)% , protease was 9(27)% and Actinomycetes spp and Erwinia spp 0% finally in bacitracin was 8(32)% E.coli . Aqueous suspensions of the dried Allium sativum (Liliaceae) bulbs extract was screened for its antimicrobial activity using the agar-well diffusion method. The highest zone of inhibition was estimated with the highest concentration of aqueous suspension in Bacillus cereus was (17 mm) followed by lower inhibition zone in Enteroabacter cloacae was (5mm). It is tested against Gram-positive bacteria and gram-negative bacteria .The suspensions were tested at concentrations of 1, 10, 100 and 1000 μg/ml when using garlic extract as antimicrobial substances, salvia as inhibition factor, and inhabited zone of saliva in Micrococcus spp was 10 mm. Also inhibited adhesion bacterial on epithelial cell by saliva was E.coli group B from 68.5±30.3 to 40.2±18.1 and in group B Staphylococcus aureus inhibited bacteria on epithelial cell from 50.4±1.4 to 30.3±9.3 , conclusion soil contain many bacteria have some virulence factor and use saliva as inhibited adhesion factors


Article
Differentiation Between Two Isolates Of Entamoeba histolytica Isolated From Different Clinical cases After Intraceacal Inoculation In Hamsters

Author: Raja ,a.AL -.Alousi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 2 Pages: 199-203
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The aim of this study is to differentiate between virulence of two isolates ,one was isolated fromsevere clinical symptoms of amebiasis isolate (A) the other was from asymptomatic carriers isolate(B).METHODS :12 male hamsters were employed in this study , they were divided into 3 groups . 1st group (5hamsters), inoculated intracecally with isolate isolate (A) from human case of severe amebiasis . 2ndgroup (5 hamsters) inculated with isolate (B) from asymptomatic carriers .3rd group (2hamsters)inoculated with saline solution as control group .RESULTS :1st group of hamsters showed a flask shaped ulcer with sever inflammation of cecum with liverabscesses , after four weeks of infection The 2nd group showed only mild inflammation of cecumwithout ulceration and liver abscess. While 3rd group were normal control group.CONCLUTION:Our study may be Considered as a step for Characterization of Entamoeba histolytica that cause theinvasive intestinal and extraintestinal amebiasis and E.dispar an intestinal commensal parasit in Iraq.


Article
Molecular Detection of Virulence Factors Genes in Pseudomonas aeruginosa Isolated from Different Infections Cases in AlDiwaniyaHospital

Author: Wafaa A. J. Al-Kaaby
Journal: journal of al-qadisiyah for pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2015 Volume: 2 Issue: 20 Pages: 53-58
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Pseudomonasaeruginosa is the most important and dangerous organism in humaninfections due to production of several extracellular and cell-associated virulence factors thatcause severe tissue damage, the most important virulence factors including (exoenzyme S,exotoxinA and lipoprotein), these factors was encoded by (exoS, toxA and oprIgenerespectively). In this study we used polymerase chain traction technique (PCR) for detectionvirulence factor genes producing by P.aeruginosa that isolated from wound, burn andpulmonary tract infections patient swab samples. The PCR results was show that all P.aeruginosa isolates was carried virulence factors genes with difference in prevalence betweenthem. The burn infection P. areuginosa isolates were show high prevalence of virulencefactors genes more than wound and pulmonary tract infections isolates as well as thevirulence factor gene (ToxA) was show high production in most isolates. In this study weconcluded that the production of virulence factors genes in P. aeruginosa is important tohuman infection especially (ToxA) gene and the PCR technique is very specific and fastmethod in detection virulence factor genes in P. areuginosa .


Article
Study Some Virulence Factors of Escherichia coli isolated from Clinical cases and animal origin foods in Babylon province
دراسة بعض عوامل الضراوة لبكتريا Escherichia coli المعزولة من اغذية حيوانية المصدر وحالات سريرية في محافظة بابل

Authors: الهام سعيد بنو --- محمد حسن عبد الكاظم
Journal: journal of the college of basic education مجلة كلية التربية الاساسية ISSN: 18157467(print) 27068536(online) Year: 2016 Volume: 22 Issue: 96/ علمي Pages: 71-82
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Isolates of Escherichia coli were collected from stool of patients with sever diarrhea for both sexes and different ages in Babylon Province hospitals,and also from ground beef meat and localy raw cheeses from different regions in Babylon Province. All isolates were identified according to morphological and biochemical tests and results were confirmed by Api 20E system. Isolates showed two types of adherence factors, 104 Isolates( 71.72%) CFA/I, 77 isolates(53.10%) CFA/III, 109 Isolates(57.17%)showed the ability to produce Biofilm, 122 Isolates(84.13%) produced Beta-Lactamase, 66 Isolates(45.51%) produced hemolysin

تم عزل وتشخيص 145 عزلة من بكتريا Escherichia coli ، 77 عزلة من براز مرضى مصابين بالاسهال من مختلف الفئلت العمرية ومن كلا الجنسين من بعض مستشفيات محافظة بابل، و(33) عزلة من عينات لحم بقري مفروم و(35) عزلة من عينات جبن محلي الصنع من مناطق مختلفة في محافظة بابل ،وشخصت العزلات وفق الاختبارات المظهرية والكيموحيوية فضلا عن استخدام نظام Api 20E.. اذامتلكت العزلات قيد الدراسة عوامل الضراوة وهي عاملي الاستعمار الاول والثالث، 104عزلة وبنسبة71.72 % CFA/I و77 عزلة وبنسبة 53.10%CFA/III ، عامل الغشاء الحيوي109 عزلة 75.17 %،عامل البيتالاكتميز 122 عزلة84.13%،وعامل الفا- الهيمولايسين66 عزلة %45.51.


Article
Genetic Comparative Study of Staphyolococcus aureus& Staphylococcus epidermidis Isolated from Wounds, Burns and Skin Flora
دراسة وراثية مقارنة لبكتريا المكورات العنقودية الذهبية و العنقوديه البشرونيه المعزولة من أخماج الجروح و الحروق و الفلورا الطبيعية

Authors: Hala S. Abdul-Kareem هالة سالم عبد الكريم --- Ali S. Husain علي صالح حسين
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1C Pages: 708-712
Publisher: Baghdad University جامعة بغداد

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Abstract

One hundred specimens from wounds, burns, and skin swabs were collected from patients laying and attended to Balad general hospital. It was found that 50 isolates belong to Staphylococcus spp., 38 isolates were identified as S. aureus and 12 isolates were identified as S. epidermidis according to microscopic, cultural and biochemical testing. The study of seven extracellular enzyme as virulence factors including the enzymes: urease, lipase, DNase, haemolysin, coagulase, β-lactamase, and lecithinase. Reavealed that 100% of S.aureus had the ability to produce these enzymes, while S. epidermidis isolates were unable to produce the enzymes DNase, lipase, coagulase, but they were capable to produce haemolysin, urease, lecithinase, and β-lactamase, the range for production of these factors were 58.33 %,80,%, 10,%, and 45% respectively.Eighteen Staphyolococcus isolates were selected according of their ability for production most of studies virulence enzymes for detection of genes encoding for the enzymes heamolysine (hly) and coagulase (coa) by using of polymerase chain reaction (PCR) technique. The Gene hIy was detected in all isolates and coa was detected in 14 isolates only

جُمعت 100 عينة من أخماج جروح وحروق ومسحات جلدية من مرضى مراجعين وراقدين في مستشفى بلد العام , وبواقع 50مسحة من الجروح و 25 مسحة من الحروق و 25 مسحة من جلد المرضى . وجد ان 50 عزلة تعود لجنس Staphylococcus منها 38 عزلة تم تشخيصها على انها S. aureus و 12 عزلة تعود الى النوع S. epidermidis , شُخصت العزلات أعتماداً على الصفات المظهرية والزرعية والاختبارات الكيموحيوية . بينت دراسة أنتاجية بعض عوامل الفوعة و لسبعة إنزيمات خارج خلوية شملت كل من الإنزيمات : urease, lipase, DNase, haemolysin, Coagulase, β –lactamase, lecithinase أن كل عزلات S.aureus كانت لها القدرة على أنتاج كل تلك العوامل وبنسبة %100 , بينما كانت عزلات S.epidermidis غير منتجة للإنزيمات DNaseو coagulaseو lipase, لكنها أنتجت كل من انزيمات haemolysin,urease,lecithinase,β-lactamase وبنسب انتاج تراوحت %58.33,%80,%10.%45 على التوالي. انتخبت 18 عزلة من عزلات staphylococci اعتمادا على قدرتها في أنتاج أغلب عوامل الفوعة لتقيم وجود الجينات المشفرة للانزيمات , coagulase(coa) (haemolysin(hIy بواسطة تقنية تفاعل البلمرة التضاعفي المتسلسل polymerase chain reaction(PCR) .تبين وجود الجين hIy في جميع العزلات المدروسة ,بينما تواجد الجين coa في 14 عزلة فقط .


Article
Virulence factors genes and phylogenic groups of Escherichia coli isolated from High Vagina and Endo-cervix of Women from Kerbala

Authors: Enaas K .AL-Khalide --- Mohannad Mohsin Ahmed --- Zuhair Hameed Abood
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2015 Volume: 8 Issue: 2 Pages: 2292-2296
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Eschrechia coli (E. coli) is the most common colonizing bacteria of the genitaltract however, its status as a cause of genital tract infections is questionable.Aims of the study: To study the virulence and phylogenic groups of E coli strains isolated from high vagina and endo-cervix.Materials and Methods: A total of 100 female patients were enrolled in this study. All patients were attendants of the Gynecology and Obstetrics Teaching Hospital in Kerbala , holy kerbala. Iraq in the period from December 2013 to January 2015. E. coli isolates were identified by standard methods. Molecular diagnosis and characterization for virulence and phylogenic genes were detected by Multiplex PCR. Results and Discussion: Among 65 isolates recovered in this study, the most frequent phylogenic group of E. coli was "B2" which comprised 61.5% (no= 40) followed by "Phylogenic group A" that comprised 33.8% (no= 22). Previous studies have shown that phylogenetic groups B2 and D are virulent because these groups are associated with the presence of several virulence factors. Interestingly, 9 out of 10 isolates from women who their husband using condoms were shown to be of B2 phylogenic group. Frequencies of virulence genes of adhesion Pap, Sfa and Afa, among E.coli isolates were 14, 42 and 5, respectively. In addition, Pap C and Sfa were found in higher rates in phylogenetic group B2 isolates, and this gives more support to the hypothesis that B2 is the pathogenic group. Conclusions: Most of the E. coli isolates were belong to the virulent phylogenic group “B2” and that those isolates harbor several virulence factors. Accordingly, E coli may be considered as a true genital tract pathogen and its colonization entails great risk for vertical transmission to the fetus and fetal membrane.


Article
Genotyping of Staphylococcus aureus Isolates Based on Methicillin-Resistance Genes and its Relatedness to some Putative Virulence Factors
التنميط الوراثي لعزلات بكتريا المكورات العنقودية الذهبية على اساس الموروثات المشفرة للمقاومة للمثيسلين وعلاقتها ببعض عوامل الضراوة الشائعة

Authors: Nuha. J. Kandala نهى جوزيف قندلا --- Mahammad Abdulateef محمد عبد اللطيف --- Nadia Imad ناديه عماد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 2A Pages: 626-638
Publisher: Baghdad University جامعة بغداد

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Abstract

The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) was a public health problem worldwide that causes nosocomial and community infections. Forty three isolates (71.66%) were characterized as S.aureus, were isolated from 60 different clinical specimens (blood, nose, wound, urine and vaginal) collected from patients from different hospitals of Baghdad. All isolates were resistant (100%) to Aztreonam, Carbenicillin, Cifixime, Cefoxitin, Ceftazidime, and showed high resistance to each of Methicillin, Oxacillin, Ampicillin and Penicillin . the MRSA isolates were typed based on (SCCmec) typing ,the result revealed that SCCmecIVa was the most common in isolates (41.86%), following type IVc (20.93%), type II(16.27%). Virulence factors were identified to detect genes encoding for Hlg,Pvl,ClfA,Tsst-1 and Eta, and the result showed the most prevalent gene was hlg (65.11%), following pvl (53.48%), clfA (51.16%), tsst-1(18.60%),eta (11.62%). The virulence genes profiles were observed, and the most frequent was clfA-hlg-pvl (23.25%), clfA-hlg-pvl-tsst-1 (6.97%),clfA-hlg-tsst-1(4.65%).Analysis of genetic similarity relationship, showed the isolates of S.aureus were classified into two main clusters . This result indicates that there a diversity in virulence genes profiles among MRSA isolates according to SCCmec types, and SCCmec IVa carried hlg, pvl , clfA genes was the most prevalent in Baghdad hospital isolates .

يعد انتشار العزلات المقاومة للمثسيلين من بكتريا المكورات العنقودية الذهبية مشكلة صحية خطيرة في العالم مسببة ظهور اصابات في المستشفيات والمجتمع تم الحصول على 43 %(71.66) عزلة بكتيرية تعود الى بكتريا S. aureus من مجموع 60 عينة, جمعت من عينات سريريه مختلفة شملت (الدم, الانف, الجروح, الادرار, المهبل) وبنسب تواجد مختلفة كانت % (60, 66.66, 71.42, 77.77, 90) على التوالي, من مستشفيات مدينة بغداد .أجري اختبار فحص الحساسية لبكتريا S. aureus تجاه 19 مضادﺁ حيويآ , اظهرت النتائج أن جميع العزلات كانت مقاومة (%100) للمضادات Aztreonam Carbenicillin, Cifixime Cefoxitin, Ceftazidime, . واظهرت هذة العزلات مقاومة عالية لكل من المضادات الاتية Methicillin, Oxacillin, Ampicillin Penicillin . أجري التحليل الجزيئي لانواع SCCmec لعزلات MRSA باستخدام 8بادئات متخصصة وقد أظهرت النتائج أن النوع SCCmecIVa (% 41.86)هو أكثر شيوعآ في عزلاتMRSA ويتبعه النوع type IVc (% 20.93) (%16.27) type II,والانواع type III, type IVd, typeVبنسبة (%2.32) , بينما لم يتم الكشف عن النوع typeI والنوع typeIVd في عزلات MRSA. أجري التحليل الجزيئي للمورثات المشفرة لعوامل الضراوة ا باستخدام 5 بادئات متخصصة للمورثات المشفرة إلى Pvl, Hlg, Eta, ClfA, Tsst-1 . أنتجت هذة البادئات حزمة واحدة ذي وزن جزيئي(433bp) للمورث المشفر pvl و535bp)) hlg eta (190bp), clfA(292bp), و 559bp)) TSST-1.أوضحت النتائج أن المورث المشفر hlg هو اكثر انتشارآ (%65.11)مقارنة مع المورثات المشفرة لعوامل الضراوة الاخرى (pvl clfA,,TSST-1 eta) حيث اظهرت انتشارآ بنسب 11.62, 18.60, 51.16, 53.48)) %على التوالي. تم التحري عن العلاقة بين الانواع SCCmec لعزلات MRSA والمورثات المشفرة لعوامل الضراوة , أوضحت النتائج بأن انتشار المورثات المشفرة لعوامل الضراوة كان مختلفآ ضمن انواع عزلات SCCmec واكثر الانواع شيوعا clfA-hlg-pvl (23.25%), clfA-hlg-pvl-tsst-1 (6.97%),clfA-hlg-tsst-1(4.65 . أوضحت نتائج التحليل الوراثي لعلاقة التشابه بين العزلات والتي قسمت الى مجموعتين اساسية بأن هنالك تنوع في المورثات المشفرة لعوامل الضراوة ضمن عزلات MRSA اعتمادآ على الانواع SCCmec , حيث وجدت أن معظم عزلات النوع SCCmec Iva تحمل أكثر من مورث مشفر للضراوة وأن معظم عزلات النوع SCCmec IVc تحمل مورثآ واحدآ , بينما معظم عزلات النوع SCCmec II لم تحمل مورثات مشفرة للضراوة .أشارت النتائج بأن النوع أكثر أنتشارآ كان النوع SCCmec Iva الحامل للمورثات المشفرة لعوامل الضراوة hlg, pvl, clfA في عزلات مستشفيات بغداد.


Article
Isolation of Pathogenic Escherichia coli O78:K80 Serotype From Broiler Chicks with Spontaneous Pathological Conditions in Basra Province

Authors: Ali A.S.AL-Mayah --- Rajaa A.Z .Ali
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2015 Volume: 6 Issue: 1 Pages: 109-117
Publisher: University of Kufa جامعة الكوفة

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Abstract

Escherichia coli (E. coli) is responsible for a variety of disease conditions which cause high economic losses in poultry due to high mortalities, decreased food conservation rate and condemnation of whole affected carcass or organs after slaughter. In the present study, 50 out of 70 broiler birds which have been collected from diseased chickens were exhibited lesions of fibrinous perihepatitis, fibrinous pericarditis and/or airsacculitis .Birds with these lesions were subjected for bacteriological examination.. The examination revealed isolation of 23 E. coli isolates with incidence of 46%. Concerning the virulence factors , the Congo red binding activity of these isolates reveled detection of 6 positive isolates with incidence of 26.08%. Serotyping showed that out of 6 Congo red positive strains, only 3 strains were serologically typed and were belonged to the serotype O78:K80. Detection of pathogenic E. coli serotypes was confirmed by PCR technique with specific primers for fimA and fimH genes. All these 3 isolates of the serotype O78:K80 were reacted with these 2 genes. Antibiogram suscebtibility pattern displayed sensitivity of these isolates to Chloramphinicol and Gentamicin and their resistance to Amoxicillin ,Erythromycin and Nalidixic acid.

Keywords

Escherichia coli --- serotyping --- virulence --- PCR


Article
Antibiotic resistance patterns and adhesion ability of uropathogenic Escherichia coli in children

Author: Hadi R. R. Al-Taai1 , Zaman A. S. Al-Jebouri1 , Ban H. Khalaf2 , Yasir Q. Mohammed1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 18-26
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to isolation and characterization of E.coli from Children urine , the phenotypic detection of biofilm formation , bacteria ability to adhesion, and the detection of fimH gene using PCR-based molecular diagnostic methods. In order to isolate E.coli, 250 urine samples were collected from children in camp saad of displaced / Diyala.Fifteen isolates(30%) of Escherichia coli were recovered according to the bactereiological , biochemical tests , used Api20E system and VITEK 2 system for confirmation. The study found that bacterial growth in females higher than in males and the incidence of infection in children under the age of 3 years is more common than in older age groups. The sensitivity test was performed for Ten antibiotics (Ampicillin, Piperacillin/tazobactam, Cefoxitin, Cefixime, Ceftazidime, Imipenem, Amikacin, Gentamicin, Ciprofloxacin, Trimethoprim /sulfamethoxacole) Where the percentage of resistance were (93.3% , 20.0% , 26.7% , 80.0% , 80.0% , 0.0% , 0.0% , 46.7% , 20.0% , 73.3%) respectively.Minimal inhibitory concentrations (MIC) were determined for Ceftazidime,Piperacillin /tazobactam, Gentamicin and Trimethoprim/ sulfamethoxacole. The MIC values were (4-32), (4-128), (<16), (20-320)μg / mL.Some of the virulence factors were detected, that included detection of biofilm formation and isolates ability to adhesion, all isolates showed ability to produce a biofilm and adhesion .Gene fimH was detected by Polymerase Chain Reaction(PCR), the percentage of isolates containing the gene was 100%.


Article
Isolation, identification and detection of some virulence factors in yeasts from local cheese in Mosul city
عزل وتوصيف وتحديد بعض عوامل الفوعة للخمائر من عينات الجبن المحلي في مدينة الموصل

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Abstract

Fifty samples of local cheese were purchased from Mosul city markets during the period from April 2012 to November 2013 to identify and characterize yeast species in these samples. Fifty-eight yeast isolates were identified and confirmed biochemically. They were Candida albicans (13.8%), Candida krusei (9.6%), Candida tropicalis (10.3%), Candida parapsiloosis (25.8%), Geotrichum candidum (20.6%), Rodotorella spp. (10.3%) and mixed yeasts (12.9%). Virulence factors (Hemolytic, Phospholipase, Aspartylprotinase and Estrase activities) of Candida isolates were determined. All isolates show one or more of these activities except Candida krusei. The presence of Candida isolates in this type of cheese refer to the importance of this genus to the health of consumers was discussed.

تم فحص خمسون عينة من عينات الجبن محلية الصنع والتي تم جمعها من اسواق مدينة الموصل خلال الفترة من نيسان 2012 ولغاية تشرين الثاني 2013 لتحديد وتوصيف أنواع الخمائر في هذه العينات. ثمانية وخمسون عزلة من الخمائر تم عزلها وأكدت بالفحوص الكيميائية. شكلت Candida albicans (13,8%)، Candida krusei (9,6%)، Candida tropicalis (10,3%)، Candida parapsiloosis (25,8%)، Geotrichum candidum (20,6%)، Rodotorella spp. (10,3%)، والخمائر المختلطة (12,9%). تم تحديد عوامل الفوعة (Hemolytic, Phospholipase, Aspartyl and Estrase) من عزلات المبيضات. اظهرت كل العزلات فعالية لواحد أو أكثر من هذه العوامل ماعدا Candida krusei. تمت مناقشة أهمية وجود جنس المبيضات في هذا النوع من الاجبان على صحة المستهلكين.

Keywords

Isolation --- Virulence factors --- Yeasts --- Cheese

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