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Article
Study of virulence factors and inhibitory effect of salvia on identified gram positive and gram negative isolated from soil

Author: Zahrah Adnan Dikhel
Journal: Misan Journal of Acodemic Studies مجلة ميسان للدراسات الاكاديمية ISSN: 1994697X Year: 2017 Volume: 16 Issue: 32 Pages: 104-116
Publisher: Misan University جامعة ميسان

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Abstract

Soil is considered one of the most suitable environmental for microbial growth . In this study and 130 bacterial isolates were from soil in musian city the first most common ratio was Escherichia .coli 30 (23.07) % and less of occurrence ratio was Citrobacter freundii 1(0.76) % also study some virulence factors like heamolysin the high ratio was Enterobacter spp 10(4)% and low ratio in Citrobacter freundii 1(1)% , protease was 9(27)% and Actinomycetes spp and Erwinia spp 0% finally in bacitracin was 8(32)% E.coli . Aqueous suspensions of the dried Allium sativum (Liliaceae) bulbs extract was screened for its antimicrobial activity using the agar-well diffusion method. The highest zone of inhibition was estimated with the highest concentration of aqueous suspension in Bacillus cereus was (17 mm) followed by lower inhibition zone in Enteroabacter cloacae was (5mm). It is tested against Gram-positive bacteria and gram-negative bacteria .The suspensions were tested at concentrations of 1, 10, 100 and 1000 μg/ml when using garlic extract as antimicrobial substances, salvia as inhibition factor, and inhabited zone of saliva in Micrococcus spp was 10 mm. Also inhibited adhesion bacterial on epithelial cell by saliva was E.coli group B from 68.5±30.3 to 40.2±18.1 and in group B Staphylococcus aureus inhibited bacteria on epithelial cell from 50.4±1.4 to 30.3±9.3 , conclusion soil contain many bacteria have some virulence factor and use saliva as inhibited adhesion factors


Article
Study Some Virulence Factors of Escherichia coli isolated from Clinical cases and animal origin foods in Babylon province
دراسة بعض عوامل الضراوة لبكتريا Escherichia coli المعزولة من اغذية حيوانية المصدر وحالات سريرية في محافظة بابل

Authors: الهام سعيد بنو --- محمد حسن عبد الكاظم
Journal: journal of the college of basic education مجلة كلية التربية الاساسية ISSN: 18157467(print) 27068536(online) Year: 2016 Volume: 22 Issue: 96/ علمي Pages: 71-82
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Isolates of Escherichia coli were collected from stool of patients with sever diarrhea for both sexes and different ages in Babylon Province hospitals,and also from ground beef meat and localy raw cheeses from different regions in Babylon Province. All isolates were identified according to morphological and biochemical tests and results were confirmed by Api 20E system. Isolates showed two types of adherence factors, 104 Isolates( 71.72%) CFA/I, 77 isolates(53.10%) CFA/III, 109 Isolates(57.17%)showed the ability to produce Biofilm, 122 Isolates(84.13%) produced Beta-Lactamase, 66 Isolates(45.51%) produced hemolysin

تم عزل وتشخيص 145 عزلة من بكتريا Escherichia coli ، 77 عزلة من براز مرضى مصابين بالاسهال من مختلف الفئلت العمرية ومن كلا الجنسين من بعض مستشفيات محافظة بابل، و(33) عزلة من عينات لحم بقري مفروم و(35) عزلة من عينات جبن محلي الصنع من مناطق مختلفة في محافظة بابل ،وشخصت العزلات وفق الاختبارات المظهرية والكيموحيوية فضلا عن استخدام نظام Api 20E.. اذامتلكت العزلات قيد الدراسة عوامل الضراوة وهي عاملي الاستعمار الاول والثالث، 104عزلة وبنسبة71.72 % CFA/I و77 عزلة وبنسبة 53.10%CFA/III ، عامل الغشاء الحيوي109 عزلة 75.17 %،عامل البيتالاكتميز 122 عزلة84.13%،وعامل الفا- الهيمولايسين66 عزلة %45.51.


Article
Antibiotic resistance patterns and adhesion ability of uropathogenic Escherichia coli in children

Author: Hadi R. R. Al-Taai1 , Zaman A. S. Al-Jebouri1 , Ban H. Khalaf2 , Yasir Q. Mohammed1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 18-26
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to isolation and characterization of E.coli from Children urine , the phenotypic detection of biofilm formation , bacteria ability to adhesion, and the detection of fimH gene using PCR-based molecular diagnostic methods. In order to isolate E.coli, 250 urine samples were collected from children in camp saad of displaced / Diyala.Fifteen isolates(30%) of Escherichia coli were recovered according to the bactereiological , biochemical tests , used Api20E system and VITEK 2 system for confirmation. The study found that bacterial growth in females higher than in males and the incidence of infection in children under the age of 3 years is more common than in older age groups. The sensitivity test was performed for Ten antibiotics (Ampicillin, Piperacillin/tazobactam, Cefoxitin, Cefixime, Ceftazidime, Imipenem, Amikacin, Gentamicin, Ciprofloxacin, Trimethoprim /sulfamethoxacole) Where the percentage of resistance were (93.3% , 20.0% , 26.7% , 80.0% , 80.0% , 0.0% , 0.0% , 46.7% , 20.0% , 73.3%) respectively.Minimal inhibitory concentrations (MIC) were determined for Ceftazidime,Piperacillin /tazobactam, Gentamicin and Trimethoprim/ sulfamethoxacole. The MIC values were (4-32), (4-128), (<16), (20-320)μg / mL.Some of the virulence factors were detected, that included detection of biofilm formation and isolates ability to adhesion, all isolates showed ability to produce a biofilm and adhesion .Gene fimH was detected by Polymerase Chain Reaction(PCR), the percentage of isolates containing the gene was 100%.


Article
Isolation, identification and detection of some virulence factors in yeasts from local cheese in Mosul city
عزل وتوصيف وتحديد بعض عوامل الفوعة للخمائر من عينات الجبن المحلي في مدينة الموصل

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Abstract

Fifty samples of local cheese were purchased from Mosul city markets during the period from April 2012 to November 2013 to identify and characterize yeast species in these samples. Fifty-eight yeast isolates were identified and confirmed biochemically. They were Candida albicans (13.8%), Candida krusei (9.6%), Candida tropicalis (10.3%), Candida parapsiloosis (25.8%), Geotrichum candidum (20.6%), Rodotorella spp. (10.3%) and mixed yeasts (12.9%). Virulence factors (Hemolytic, Phospholipase, Aspartylprotinase and Estrase activities) of Candida isolates were determined. All isolates show one or more of these activities except Candida krusei. The presence of Candida isolates in this type of cheese refer to the importance of this genus to the health of consumers was discussed.

تم فحص خمسون عينة من عينات الجبن محلية الصنع والتي تم جمعها من اسواق مدينة الموصل خلال الفترة من نيسان 2012 ولغاية تشرين الثاني 2013 لتحديد وتوصيف أنواع الخمائر في هذه العينات. ثمانية وخمسون عزلة من الخمائر تم عزلها وأكدت بالفحوص الكيميائية. شكلت Candida albicans (13,8%)، Candida krusei (9,6%)، Candida tropicalis (10,3%)، Candida parapsiloosis (25,8%)، Geotrichum candidum (20,6%)، Rodotorella spp. (10,3%)، والخمائر المختلطة (12,9%). تم تحديد عوامل الفوعة (Hemolytic, Phospholipase, Aspartyl and Estrase) من عزلات المبيضات. اظهرت كل العزلات فعالية لواحد أو أكثر من هذه العوامل ماعدا Candida krusei. تمت مناقشة أهمية وجود جنس المبيضات في هذا النوع من الاجبان على صحة المستهلكين.

Keywords

Isolation --- Virulence factors --- Yeasts --- Cheese


Article
Virulence Factors of Proteus Mirabilis Isolated From PatientsOtitis Media in Baquba And it’s Peripheries

Authors: Abdul-Razak SH. Hasan عبد الرزاق شفيق حسن --- Nadhum G. Nauman عباس الدليمي --- Abbas A. Al-Duliami عباس الدليمي
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2011 Volume: 1 Issue: 1 Pages: 69-75
Publisher: Diyala University جامعة ديالى

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Abstract

The objective of this study is to evaluate the impact of prostate-specific antigen density ( PSDA ) when serum levels of prostate-specific antigen ( PSA ) are less than 10 ngml in predicting prostate cancer. We retrospectively analyzed 134 patients who underwent trans rectal ultrasound ( TRUS )-guided prostate biopsies according to Cooner's algorithm. Histopathological examination revealed prostate cancer ( PCa ) in 22 ( 16% ) and begin prostatic hypertrophy ( BPH ) in 112 ( 84% ) patients.Five patients ( 23% ) with PCa had PSAD<0.15 of whom 3 had PSA<4 ngml and 2 had PSA between 4 and 10 ngml. In the BPH group, 60 patients ( 54% ) had PSAD below 0.15 whereas 52 patients ( 46% ) had PSAD over 0.15 . with 0.15 as the cutoff level of PSAD, the sensitivity and specificity of PSAD was found as 77 and 54%, respectively. In this patient population, PSA with the cutoff level of 4 ngml has sensitivity and specificity level of 77 and 33% respectively. According to these result, a statistically significant difference was found between PSA and PSAD only in terms of diagnostic specificity ( chi-square, P<0.05 ) . There were 29 patients with negative digital rectal examination ( DRE ) and TRUS and PSA 4-10 ngml who underwent biopsy because of PSAD>0.15 . no cancer was detected in this group of patients, suggesting that biopsy in this subgroup may be unnecessary.


Article
Determination of some Virulence Factors of Pasteurella multocia isolated from Human and Farm Animals infections
تحديد بعض عوامل الضراوة لبكتريا Pasteurella multocida المعزولة من إصابات الإنسان والحيوانات الحقلية

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Abstract

Twenty one Isolates of Pasteurella multocida were obtained from different clinical specimens of human and farm animals' infections. Human specimens included wounds swabs taken after cats and dog's bits beside the urine and sputum samples. The animal samples were nasal swabs and blood samples taken from chattels, also the poultry tissues of infected chicken with fowl cholera were collected. Bacterial isolates were isolated using Pasteurella multocida selective agar (PMSA) then identified doing different morphological, biochemical tests followed by api 20E diagnosis. The ability of the bacterial isolates to produce different virulence factors were studied, 18 isolates 85.7% were able to produce Lipase enzyme. Results of pathogenicity study on Lab. animals (mice) showed that there were 9 highly virulent isolates among the 21 (42.8%). The killing time was in between (10-24) hrs after injected the mice’s intraperitoneally. Some of the isolates showed their ability to produce the dermonecrotic toxin and the positive result appeared as highly purulent, dermonecrotic lesions after injection Genia pig intradermally. Results of antibiotic sensitivity test revealed that there were considerable variations in isolates susceptibility. Some of the isolates were highly resisted most of the used antibiotics while others were not. The crude bacterial extract from PMA20 isolate was tested to determine its ability to stimulate human lymphocytes division in vitro, results showed that the extract was able to stimulate cells division when the mitotic index was 1.2% as compared with the positive control.

أمكن الحصول على 21 عزلة من بكتريا Pasteurella multocida من نماذج مختلفة شملت مسحات من الجروح الناجمة عن عضات الكلاب والقطط وعينات القشع والادرار من الإنسان فيما شملت العينات الحيوانية أخذ مسحات الأنف وعينات من دم قطعان الماشية فضلا عن اخذ عينات من أنسجة دواجن ميتة مصابة بكوليرا الدجاج . عزلت البكتريا باستخدام وسط اكار الباستورلا-مالتوسيدا الانتخابي ثم شخصت اعتمادا على عدد من الفحوص المورفولوجية والبايوكيميائية فضلا عن التشخيص بنظام api 20E . تم التحري عن قابلية العزلات عن انتاج عدد من عوامل الضراوة اذ أظهرت النتائج قابلية 18 عزلة 85.7% على إنتاج الأنزيم المحلل للدهن Lipase كما بينت الدراسة أن هنالك 9 عزلات شديدة الضراوة للفئران من مجموع 21 (42.8%) ، وقد تراوح وقت الهلاك ما بين (24-10) ساعة بعد حقن الحيوانات بغشاء البريتون . استطاعت بعض من العزلات من إنتاج السموم المنخرة للجلد ، وظهرت النتيجة الموجبة من خلال حصول تقيح وتنخر شديدين بعد حقن خنازير غينيا تحت الجلد . فيما يخص نتائج فحص الحساسية للمضادات الحيوية فقد ظهر هنالك تفاوت في استجابة هذه العزلات اذ قاومت بعض منها وبشدة اغلب المضادات المستعملة في الوقت الذي استجابت بعض منها لهذه المضادات . اختبر المستخلص الخام للعزلة PMA20 للتحري عن قابليته لتحفيز خلايا دم الإنسان اللمفاوية على الانقسام في الزجاج اذ بينت النتائج قدرته المحفزه وبلغ معامل الانقسام %1.2 مقارنة مع نموذج السيطرة .


Article
Virulence factors of enterococci species isolated from nosocomial and community acquired infections

Authors: Abbas A. Al-Duliami --- Nadhum G. Nauman --- Afak R. Salman --- Abdul-Razak SH. Hasan
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 3 Pages: 174-182
Publisher: Diyala University جامعة ديالى

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Abstract

Several virulence and pathogenicity factors have been described from enterococci thatenhances their ability to colonize patient's tissues, increase resistance to antibiotics, andaggravate the infection outcome. The present study aimed to investigate virulence andpathogenicity factors among enterococci species isolated from nosocomial and communityacquired infection in Diyala. The study was conducted in Baquba General Hospital and Al-Batool Hospital for Maternity and children during the period from 1st. September/2005 to 30th.September /2006. A total of 343 specimens were collected from 213 inpatients and 130outpatients. 200 (58.3%) were females and 143 (41.7%) were males. The mean age of patientswas (32.8 ± 17.2) years. 44 isolates of enterococcal species were recovered from differentclinical specimens and identified according to standard bacteriological and biochemicalcriteria. The presence of certain virulence and pathogenicity factors, namely; gelatinase andhemolysin production, biofilms formation, agglutination of erythrocytes, presence of capsule,and adherence to epithelial cells were detected. Data were statistically analyzed.The results showed that all isolates of E. gallinarium and E. avium were biofilmformer compared to 76.7% and 70% of E. faecalis and E. faecium respectively. Furthermore,all isolates of E. gallinarium and 76.7% of E. faecalis were β-lactamase producer.Additionally, all isolates of E. avium and 76.7% of E. faecalis were agglutinated RBCs. Thepresence of capsule was highest among E. faecalis isolates (26.7%). The results also revealedthat all E. galinarium and E. avium isolates were non-hemolytic. Furthermore, among 12isolates which express β- hemolysis, 10 (33.3%) and 2 (20%) were E. faecalis and E. faeciumrespectively. α-hemolysis were found among 10 (26.7%) isolates of E. faecalis and 2 (20%)isolates of E. faecium. It can be concluded that Local isolates of enterococci species recoveredfrom different clinical specimens are multi-virulence bacteria.


Article
Virulence factors study of Alcaligens isolated from urinary tract infection of diabetic patients
دراسة عوامل ضراوة البكتريا Alcaligenes المعزولة من إصابات المجاري البولية لمرضى داء السكر

Author: Al-Rassam , Z .T زياد ذنون الرسام
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2006 Volume: 11 Issue: 2 Pages: 247-252
Publisher: Tikrit University جامعة تكريت

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Abstract

The study showed that, all isolates of Alcaligenes were able to produce - lactamase and Slime layer while they were incapable to produce Lecithenase, Lipase, DNase and Neurotoxin. They were also exhibited a sepecific affinity to adhere to epithelial cells which were isolated from diabetic urine. Moreover , Alcaligenes isolates showed absolute resistance to several antibiotics such as Amoxcillin, Sulfonamides and Cefotaxime, while they were sensitive to Amikacin, Ciprofloxacin and Neomycin .

أظهرت نتائج الدراسة قدرة جميع عزلات بكتريا Alcaligenes على إنتاج إنزيمات -lactamase والمادة المخاطية و عدم قدرتها على إنتاج إنزيمات الليسثنيز ، اللايبيز ، البروتيز و الانزيم الحال للحامض النووي منقوص الأوكسجين والذيفان العصبي . بينت النتائج قدرة عزلات البكتريا A. faecalis على الالتصاق بسطوح الخلايا الطلائية البولية المعزولة من إدرار مرضى داء السكر دون سواهم . أبدت عزلات البكتريا Alcaligenes مقاومة تجاه العديد من المضادات الحيوية ومنها Amoxcillin و Sulfonamides و Cefotaxime ، بينما كانت حساسة تجاه المضادات الحيوية Amikacin و Ciprofloxacin وNeomycin .


Article
Detection of Virulence Factors hla and hlb of Staphylococcus aureus using PCR Technique

Author: Adil Abead Hassuny
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 4 Pages: 1000-1006
Publisher: Babylon University جامعة بابل

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Abstract

Thirty isolates were collected from patients sputum and throat swabs who admitted at Teaching General Hospital in Hilla city during a period of two months lasting from (Febraury to March 2014). The morphological characterization and biochemical reactions showed 18 isolates diagnosed as Staphylococcus aureus, of which obtain only six isolates, two from sputum samples (25%) and four from throat swabs (0.4%) have hlb gene and only four isolates, two from sputum samples(25%) and two from throat swabs (0.2%) have hla gene using PCR techniques.


Article
دراسة بعض عوامل الضراوة لبكتريا Escherichia coli المعزولة من اغذية حيوانية المصدر وحالات سريرية في محافظة بابل

Author: الهام سعيد بنو محمد حسن عبد الكاظم جامعة بغداد/ كلية التربية للعلوم الصرفة-ابن الهيثم/قسم علوم الحياة
Journal: journal of the college of basic education مجلة كلية التربية الاساسية ISSN: 18157467(print) 27068536(online) Year: 2016 Volume: 22 Issue: 96/ علمي Pages: 71-82
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Study Some Virulence Factors of Escherichia coli isolated from Clinical cases and animal origin foods in Babylon provinceIlham Saeed Banno, Mohammed Hasen AbdulkadhumBiology Department,Education College for pure sciencse-Ibn Alhaitham,Baghdad UniversityAbstract Isolates of Escherichia coli were collected from stool of patients with sever diarrhea for both sexes and different ages in Babylon Province hospitals,and also from ground beef meat and localy raw cheeses from different regions in Babylon Province. All isolates were identified according to morphological and biochemical tests and results were confirmed by Api 20E system. Isolates showed two types of adherence factors, 104 Isolates( 71.72%) CFA/I, 77 isolates(53.10%) CFA/III, 109 Isolates(57.17%)showed the ability to produce Biofilm, 122 Isolates(84.13%) produced Beta-Lactamase, 66 Isolates(45.51%) produced hemolysin.

ثبت منذ سنين عديدة بان بكتريا Escherichia coli مسببا رئيسيا لامراض الاسهال Diarrheal diseases، وحدوث العديد من الاوبئة على نطاق عالمي كان سببها تناول الغذاء والماء الملوثين بهذه البكتريا،ويعد وجودها في الماء والغذاء دليلا على التلوث البرازي اذ تستوطن القناة الهضمية للانسان والحيوان (1). تمتلك بكتريا E. coli غير الممرضة في أمعاء الأنسان البالغ أهمية خاصة وذلك لانها تقوم بدور وظيفي مهم جداً متمثلاً بالمحافظه على الفعاليه الفسلجيه ولها فوائد جمة في موازنة البيئة في أمعاء الانسان من خلال انتاجها الفيتامينات وانواع المضادات الحيوية كالكولسين والأحماض الدهنيه قصيرة السلسله (2). ونتيجة لتواجدها مع الأجناس القريبه منها والممرضه في الأمعاء، انتلقت اليها البلازميدات والعاثيات الحامله لجينات الذيفانات المعوية او عوامل الغزو، وبذلك اصبحت مفوعه (virulent) وقادرة على أحداث الالم والأسهال المائي او الديزانتري البكتيري الألتهابي وهذه الأمراض شائعة ومعروفة باسهال المسافرين وهي مشكلة صحية في الاقطار الفقيرة والنامية وبالاخص اسهال الأطفال الرضع ( 3 و4 (، وقد أشار (5) ان بكتريا E.coli المسببة للاسهال تقسم الى مجاميع ثانوية اعتماداً على عوامل الفوعة التي تمتلكها كل مجموعة، اي الصفه المميزه لها، لذلك فعندما يتم تشخيص هذه المجاميع، يجب اولاً عزل بكتريا E.coli قبل الكشف عن عوامل الفوعة المحدده لكل مجموعة التي توجد بعضها ضمن التركيب الخلوي وبعضها الاخر يفرزخارج الخلية(6) ، وعليه قسمت بكتريا E.coli المسببة للاسهال الى ست مجاميع ثانوية اعتمادا على عوامل الضراوة التي تمتلكها كل مجموعة ،أي الصفة المميزة لها وهي : المجموعة الممرضة للامعاء Enteropathogenic E.coli (EPEC) ،المجموعة الغازية للامعاء Enteroinvasive E.coli (EIEC) ، المجموعة المعوية النزفية Enterohemorrhagic E.coli (EHEC) ، المجموعة المعوية المتجمعة EnteroAggregative E.coli (EAggEC) ،المجموعة ذات الالتصاق المنتشر DiffuselyAdhering E.coli (DAEC) ،المجموعة السامة للامعاء Enerotoxigenic E.coli (ETEC) . (5 و7)تهدف الدراسة الحالية الى الحصول على عزلات لبكتريا E.coli من براز مصابين بالاسهال واغذية حيوانية المصدرفي محافظة بابل ودراسة عوامل الضراوة للعزلات.

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