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Article
EVALUATION OF SOME HEMATOLOGICAL PARAMETERS AND CLINICAL SIGNS AFTER REPEATED EXPOSURE TO WARFARIN IN DOGS
تقيم العلامات السريريه وبعض القيم الدمويه بعد التعرض المتكرر للوارفرين في الكلاب

Author: Israa Abdul Wadood Muhammad Ali
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 249-259
Publisher: Basrah University جامعة البصرة

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Abstract

Warfarin poisoning in dogs is not unusual which is used as a rodenticide. Competitive inhibition of vitamin K with an incomplete synthesis of the coagulation factors II, VII, IX and X can lead to a significant bleeding tendency. The study was conducted at college of veterinary medicine / Basrah university to Evaluate clinical ,hematological and clotting indices in dogs in case of warfarin poisoning, which include twelve dogs of both sex at age about three years old. The animals divided to three groups equally .first group treated with 3mg warfarin tablet given orally daily,the second group treated with 5mg until the signs of poisoning appears While the third group untreated as a control. The results showed that the first group exhibited signs of warfarin poisoning like hematuria, vaginal bleeding, severe eye congestion, limping, bleeding in toe, excessive salivation, severe pale of mucus membrane in gum, hemoptysis, also the second group exhibited signs of warfarin poisoning after ten days the signs was hematuria and vaginal bleeding ,the animal showed signs of severe eye congestion, depression, weakness, and lameness ,bleeding in toe and then excessive salivation ,sever pale mucous membrane in gum, hemoptysis, blood vomit. weakness, excitement,nose bleeding, eye bleeding,protruded of eye,congestion of gum, melena and incoordination. there is significant decrease (P<0.01) in mean value of red blood cell count in dog treated with 3mg warfarin (4.09 ×106 ± o.254) as well as hemoglobin level( 9.613 g/dl±o.6085 ), packed cell volume (23.133 ±0.592) . and there is increase in mean of MCV (72. 40 fl±1.29) , MCH (23.86pg±0.52) and MCHC (32.78 g/dl ±0.50 ) which indicate macrocytic anemia . Also dogs treated with 5mg warfarin showed that the mean of red blood cell count was 3.81 ×106± 0.2347, mean of Hb was 8.30 ±1.006 g/dl and PCV 28± 2.510 with significant decrease (P<0.01) in these parameters and there is significant increase in mean of MCV,MCH. and MCHC 77.07fl±1.31 , 22.74 pg±0.49, 31.38 g/dl ±0.294 respectively . There is significant increase(P<0.01) in mean of prothrombin time and activated partial Thromboplastin Time in dogs treated with 3mg of warfarin, 1.336 ± 0.146 , 1.036 ± 0.1074 min.Respectively whencompared with control animals 0.601± 0.0863 , 0 .153±0.003min. and There is significant decrease (P>0.05) in mean value of platelets count 253.80 × 103g/l ± 18.31 compared with control group 448.12 ±52.24 . ,also there is significant increase(P<0.01) in PT. and APTT. when treated with 5mg 1.855 ±0.2039 , 1.401 ±0.1051 min.respectively,whereas the mean of platelet 240.40 × 103 g/l ± 5.39 with significant differences(P<0.01) .


Article
The influence of some amino acids,vitamins and anti-inflammatory drugs on activity of chondroitinase produced by Proteus vulgaris caused urinary tract infection
تأثير بعض الأحماض الأمينية والفيتامينات وبعض العقاقير المسكنة للألام على فعالية انزيم ال chondroitinase المنتج من بكتريا proteus vulgaris المسببه لألتهاب المسالك البولية

Authors: Bahaa A. Laftaah AL-Rubaii بهاء عبدالله لفته الربيعي --- Mokhtar J. kadhim AL-Imam مختار جواد كاظم الامام
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 4A Pages: 2413-2421
Publisher: Baghdad University جامعة بغداد

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Abstract

Two hundred and ten specimens include urine, blood and ear swab were collected from different hospitals in Baghdad city; 85 (40%) isolates were diagnosed as Proteus spp. with (82%), (11.7%) and (5.8%) represented in urine, blood and ear swab specimens respectively. PCR technique was shown 30 (35.3%) isolates were positive for specific Urease C gene that used in rapid detection of Proteus vulgaris. The ability for chondritinase production was checked invetro and invevo, 24 (80%) isolates of P. vulgaris were showed ablity to chondritinase production and the isolate (p17) has higher enzyme activity value to (175.2U/ml). The Chondroitinase was purified by three short steps only included precipitate with 60% saturated of ammonium sulfate, dialysis and then filtrated by Sephadex G-150. The enzymatic activity was peaked to (270U/ml) and specific activity (692.3U/mg). The enzymatic activity for purified enzyme was increased significantly (P<0.01) to (563.7U/ml) after treatment with 1M of tyrosine but aspartic acid and glutamic acid were show inhibitory effect on enzyme activity decreased (P<0.01) to (130.U/ml) and (147.3 U/ml) respectively. The enzymatic activity was also increased significantly (P<0.01) reached to (633.6U/ml) and to(610.3U/ml) when treated with 50% of vitamin k and piroxicam respectively. This study was aimed to investigate effect of amino acids, vitamins and some nonsteroidal anti-inflammatory drugs (NSAIDs) with different concentrations on enzyme activity.

تم جمع مائتين وعشرة عينات من البول والدم ومسحات الاذن من مختلف المستشفيات في مدينة بغداد. تم تشخيص(85عزلة) من بكتريا Proteus spp. وبنسبة (40%) وشكلت عزلات الProteus spp نسب (82%) من عينات البول و(11.7%) دم و(5.8%) من مسحات الاذن على التوالي .اظهر التشخيص بتقنية تفاعل البلمرة التسلسلي PCR وجود ( 30 عزلة ) تابعة لبكتريا الProteus vulgaris بنسبه (35.3%) عند استخدام الجين التشخيصي Urease C. تم التحري عن قابلية البكتريا لإنتاج انزيم الchondroitinase واظهرت النتائج (80%) 24 عزلة من الP. vulgaris منتجة للأنزيم . وتم انتخاب العزلة ( p17) الاكفأ بالإنتاج ذات فعالية انزيمية بلغت ( 175وحدة /مل) . نقي الانزيم بثلاث خطوات قصيرة تضمنت الترسيب بأملاح كبريتات الامونيوم بنسبة اشباع بلغت 60% و الديلزة ومرر الراشح بعمود التنقية Sephadex G-150 وكانت الفعالية الانزيمية (270 وحدة/مل ) وبفعالية نوعية بلغت اقصاها (692.3 وحدة /مليغرام). ازدادت الفعالية الانزيمية للأنزيم المنقى معنويا(P<0.01) اذ بلغت ( 563 وحدة /مل) بعد معاملة الانزيم بالحامض الاميني (التايروسين) عند التركيز( 1 مولاري) بينما اظهر حامضي الاسبارتك و الكلوتامك ثأثيرا مثبطا للفعالية الانزيمية بلغت ادناها (130.2وحدة/مل) و(147.3وحدة/مل) على التوالي. كذلك ازدادت الفعالية الانزيمية معنويا (P<0.01) الى (633 وحدة/مل) و(610.3 وحدة /مل) بعد المعاملة بفيتامين K و العقار بايروكسكام بتركيز(50%) على التوالي. هدفت هذه الدراسة بيان تأثير بعض الاحماض الامينية والفيتامينات وبعض العقاقير المسكنة للآلام بمختلف التراكيز على الفعالية الانزيمية.


Article
The relationship between levels of osteocalcin, calcium, vitamin K and vitamin D in sera of patients with bone fractures compared to apparently healthy control

Author: Yasir A. Al-Sudani1 , Essam F. Al-Jumaily1 , Ban A. AbdulMajeed2 , Munwar A. Al-Naqqash3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 42-46
Publisher: Baghdad University جامعة بغداد

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Abstract

The production of osteocalcin is stimulated by 1, 25 dihydroxy vitamin D and depends on vitamin K. Vitamin K increases the carboxylation of osteocalcin, but it does not increase its overall rate of synthesis. Although its function is not completely understood, osteocalcin may exist as a deposition site for hydroxyapatite crystals. It may also affect energy metabolism via the production and action of insulin. The present study aimed at measuring serum levels of osteocalcin, calcium, vitamin k and vitamin D in sera of patients complaining from different types of fractures compared to levels in apparently healthy controls. Serum osteocalcin was within normal limits in patients and controls. Serum calcium generally lower than normal, vitamin D was lower than normal and vitamin K was within normal limits. The results indicated the importance of measurement of vitamin D and serum calcium in patients with fracture.


Article
Case Report of Chondrodysplasia Punctata: A Rare Complication of Hyperemesis Gravidarum

Author: Mahdi Abdul Sahib
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2018 Volume: 11 Issue: 2 Pages: 4064-4067
Publisher: Kerbala University جامعة كربلاء

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Abstract

Chondrodysplasia punctata is a very rare congenital disorder characterized by abnormal calcification within growing cartilage at the ends of the long bones affecting their growth. It has seen in inherited and acquired disorders. Acquired cases may be seen in infants of mothers with deranged vitamin K metabolism during pregnancy as in severe hyperemesis gravidarum. We report 2.5 years girl with features of chondrodysplasia punctata in whom maternal prenatal pregnancy history was remarkable of hyperemesis gravidarum. According to my information, this is the first recorded case in holy Karbala governorate.

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