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The main objectives of this study were to elucidate the influence of ascorbate and salicylate in alleviating oxidative stress induced cadmium toxicity in mung bean Vigna radiata L. Wilczek grown hydroponically during rooting responce. Supplying of AsA simultaneously with cadmium chloride (CdCl2) was detoxified Cd by approximately duplicating the roots number (10.9 root) compared to general control (6 root ) as well as to CdCl2 control (1 root) . Mean -while,the same rooting response was equalled to that developed by the effect of AsA alone 11.5 root . However, supplying of AsA after CdCl2 treatment (post treatment) was detoxified Cd by raising the root number to the level of control whereas ,the pretreatment with AsA didn΄t have the potency in Cd detoxification . SA treatment at any of the previous timing and the treatment with AsA+ SA did not able to detoxify Cd in cuttings which have no synergistic action between AsA and SA in alleviating Cd toxicity.
تهدف هذه الدراسة الى تبيان تاثير حامضي الاسكوربيك(AsA) و السالسليك (SA) في تخفيف الاجهاد التاكسدي المتسبب عن سمية الكادميوم خلال عملية التجذير في عقل الماش Vigna radiata L. Wilczek باستخدام تقنية المزارع المائية . ان تجهيز AsA سوية مع CdCl2 ازال السمية بمضاعفة معدل عدد الجذور تقريباً 10.9 جذر مقارنة بعينة السيطرة العامة 6 جذر و مقارنة بعينة CdCl2 1 جذر وهي مساوية معنوياً لمعدل عدد الجذور المتكشفة بتأثير AsA لوحده 11.5 جذر. اما تجهيز AsA بعد المعاملة بالكادميوم post treatmentفقد ازال سمية الكادميوم برفع معدل عدد الجذورالى العدد نفسه في عينة السيطرة العامة , في حين لم تكن للمعاملة ﺒ AsA قبل الكادميوم pre treatment القدرة على ازالة سمية CdCl2. ان استخدام SAمع أي من التوقيتات السابقة (قبل,بعد, الوقت نفسه) وكذلك بالنسبة للمعاملة AsA+SA لم تفلح في ازالة سمية اﻟ Cd في العقل اذ وجد انه ليس هناك فعل تآزري بين الحامضين في تخفيف سمية الكادميوم .
:- Adventitious root formation --- Ascorbic acid --- Auxin --- Cadmium --- Detoxification --- Mung bean --- Salicylic acid .
Abstract The role of Zinc in alleviating B-toxicity in terms of adventitious root formation (ARF) in cuttings of mung bean was studied. The toxic level of B in addition, to the promotory conc. of Znsalt was determined. Two of Zn-salts were tested (sulphate, and chloride), auxin was supplied as inductive treatment to enhance rooting response. 1. The toxic level of boron was 150 (µg/ml), this reduced growth parameters in terms of rooting response to 50% compared to control. 2. Zinc sulfate at conc. 1 (ppm) and Zinc chloride at 5 (ppm) developed the highest rooting response in mung bean cuttings. 3. For Boron detoxification, Zn-(sulphate and chloride) were supplied as pre-treatment, posttreatment or simultaneously with toxic-B. The best treatment for boron detoxification occurred completely by supplying Zinc sulphate and chloride prior to toxic-B treatment (pre-treatment), but after supplying auxin inductive treatment. 4. The protective role of Zinc-sulphate and chloride significantly enhanced the average of root number/cutting to its highest level in control treatment.
اﳋلاﺻة ﺇﻥ ﺩﻭﺭ ﺍﻟﺰﻧﻚ ﰲ ﲣﻔﻴﺾ ﺳﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ ﺑﺪﻻﻟﺔ ﺗﻜﻮﻳﻦ ﺍﳉﺬﻭﺭ ﺍﻟﻌﺮﺿﻴﺔ ﰲ ﻋﻘﻞ ﺍﳌﺎﺵ ﻗﺪ ﺩﺭﺱ ﺑﻌﺪ ﲢﺪﻳﺪ ﺍﻟﱰﻛﻴﺰ ﺍﻟﺴﺎﻡ ﻣﻦ ﺍﻟﺒﻮﺭﻭﻥ ﻭﺍﻟﱰﺍﻛﻴﺰ ﺍﳌﺤﻔﺰﺓ ﻟﻜﻞ ﻣﻦ ﻛﱪﻳﺘﺎﺕ ﻭﻛﻠﻮﺭﻳﺪ ﺍﻟﺰﻧﻚ ﺣﻴﺚ ﺟﻬﺰ ﺍﻻﻭﻛﺴﲔ ﻛﻤﻌﺎﻣﻠﺔ ﺍﺳﺘﺤﺜﺎﺛﻴﺔ ﻟﺘﺤﻔﻴﺰ ﺍﻟﺘﺠﺬﻳﺮ ﻭﻗﺪ ﺑﻴﻨﺖ ﺍﻟﻨﺘﺎﺋﺞ ﺍﻵﰐ: ( ﺣﻴﺚ ﺃﺧﺘﺰﻝ ﻣﺆﴍ ﺍﻟﻨﻤﻮﺍﳌﺘﻤﺜﻞ ﺑﻌﺪﺩ ﺍﳉﺬﻭﺭ ﺍﻟﻌﺮﺿﻴﺔ .1 1- )ﻣﺎﻳﻜﺮﻭﻏﺮﺍﻡ. ﻣﻞ150ﺍﻥ ﺍﻟﱰﻛﻴﺰ ﺍﻟﺴﺎﻡ ﻣﻦ ﺍﻟﺒﻮﺭﻭﻥ ﺍﳌﺘﻜﺸﻔﺔ ﰲ ﺍﻟﻌﻘﻞ ﺍﱃ ﺍﻟﻨﺼﻒ ﺗﻘﺮﻳﺒﺎ ﻣﻘﺎﺭﻧﺔ ﺑﻌﻘﻞ ﺍﻟﺴﻴﻄﺮﺓ. ﺃﻋﲆ ﺍﺳﺘﺠﺎﺑﺔ ﻟﺘﺠﺬﻳﺮ ﻋﻘﻞ ﺍﳌﺎﺵ. .2 (ppm) 5 ﻳﻠﻴﻬﺎ ﻛﻠﻮﺭﻳﺪ ﺍﻟﺰﻧﻚ ﻋﻨﺪ ﺍﻟﱰﻛﻴﺰ (ppm) 1ﺣﻘﻘﺖ ﻛﱪﻳﺘﺎﺕ ﺍﻟﺰﻧﻚ ﻋﻨﺪ ﺍﻟﱰﻛﻴﺰ ﺍﻭﲠﺪﻑ ﲣﻔﻴﻒ ﺳﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ ﻓﻘﺪ ﺟﻬﺰﺕ ﻛﱪﻳﺘﺎﺕ ﻭﻛﻠﻮﺭﻳﺪ ﺍﻟﺰﻧﻚ ﻗﺒﻞ ﻭﺑﻌﺪ ﺍﻟﺘﻌﺮﺽ ﻟﻠﺴﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ ﻭﺳﻮﻳﺔ .3 ﻭﻛﺎﻧﺖ ﺍﳌﻌﺎﻣﻠﺔ ﺍﻷﻓﻀﻞ ﰲ ﺍﺯﺍﻟﺔ ﺳﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ ﻋﻨﺪ ﲡﻬﻴﺰ ﺍﻟﻜﱪﻳﺘﺎﺕ ﺍﻭﻛﻠﻮﺭﻳﺪ ﺍﻟﺰﻧﻚ ﺑﻮﺟﻮﺩ ﺍﻻﻭﻛﺴﲔ ﻗﺒﻞ ﺍﻟﺘﻌﺮﺽ ﻟﻠﱰﻛﻴﺰ ﺍﻟﺴﺎﻡ ﺣﻴﺚ ﲤﺖ ﺍﺯﺍﻟﺔ ﺳﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ ﺑﺎﻟﻜﺎﻣﻞ ﻟﻜﻦ ﻣﻦ ﺧﻼﻝ ﲡﻬﻴﺰﻫﺎ ﻻﺣﻘﺎﹰ ﺑﺎﻻﻭﻛﺴﲔ. ﹰ .4 ﺍﻥ ﺍﻟﺪﻭﺭ ﺍﻟﻮﻗﺎﺋﻲ ﻟﻠﻜﱪﻳﺘﺎﺕ ﻭﻛﻠﻮﺭﻳﺪ ﺍﻟﺰﻧﻚ ﲢﻘﻖ ﻣﻦ ﺧﻼﻝ ﺍﻟﺘﺸﺠﻴﻊ ﺍﳌﻌﻨﻮﻱ ﳌﻌﺪﻝ ﻋﺪﺩ ﺍﳉﺬﻭﺭ ﺍﳌﺘﻜﺸﻔﺔ ﻭﺻﻮﻻ ﺍﱃ ﻣﺴﺘﻮﺍﻫﺎ ﰲ ﻣﻌﺎﻣﻠﺔ ﺍﻟﺴﻴﻄﺮﺓ.
Zinc --- B-toxicity --- adventitious root --- mung bean. --- ﺍﻟﺰﻧﻚ، ﺳﻤﻴﺔ ﺍﻟﺒﻮﺭﻭﻥ، ﺍﳉﺬﻭﺭ ﺍﻟﻌﺮﺿﻴﺔ، ﻋﻘﻞ ﺍﳌﺎﺵ
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