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The effects of bisphosphonate administration on teeth development and growth of the jaw bones in neonatal rats (histological and immunohistochemical study)

Authors: Suhail Labeeb Hasoon سهيل لبيب حسون --- Nada M. H. Al-Ghaban ندى الغبان
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: special issue 1 Pages: 77-82
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Bisphosphonates are potent inhibitors of osteoclastic bone resorption and widely used for the treatmentof osteoporosis, and osteogenesis imperfecta in children. Clinical and experimental studies have demonstrated thatBisphosphonates delay or inhibit tooth eruption. This study tries to focus on the effect of bisphosphonate on teethdevelopment and jaw bones growth.Materials and methods: The present study includes 65 neonatal rats during lactation period from 15 Albino Wister ratsmother. Alendronate (one type of Bisphosphonates) was administrated orally (15 mg/kg) into 10 pregnant rats twotimes a week, while other 5 rats regard as control. Then the neonatal rats sacrificed in I, 6, 11, 16 and 21 days. Thelower first molar were examined histologically and immunohistochemical for amelogenin expression. Biochemicalserum analysis for calcium and alkaline phosphatase level were down for 11, 16 and 21days group. All histological,immunohistochemical, and biochemical results are compare with their controls.Results: The histological results illustrate retardation in tooth and root development, impairment in maturation ofenamel and retardation in tooth eruption of the first molar tooth germ in alendronate treated neonatal rats than theircontrols. Also immunoreactivity for amelogenin at early stages of tooth development was somewhat more intense inexperimental group than that in their controls .Moreover, calcium and alkaline phosphatase serum levels inexperimental rats are less than that of their controls.Conclusion: This study concludes that treatment with alendronate during tooth development has the potential toinhibit tooth eruption, impair tooth formation, may induce some types of dental abnormalities, and increase thebone trabecule thickness by decreasing osteoclastic activity.


Article
USE OF TEETH SAMPLES FOR GENDERDETERMINATION BY PCR IN IRAQI CASE
PCR إستعمال عينات الأسنان لتحديد الجنس بتقنية

Author: عبد الأمير محمد غريب
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 4 Pages: 819-827
Publisher: Baghdad University جامعة بغداد

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Abstract

ABSTRACT
Identification by DNA analysis has proven valuable when the use of traditional
forensic identification methods such as fingerprints or dental radiographs is difficult
or impossible in situations such as explosions. Identification is even more difficult
because human remains are often fragmented and may be commingled. Teeth are a
useful source of DNA and can often survive extreme environmental conditions. DNA
were extracted from 10 male and 10 female teeth samples ranging in age from 24 to 47
year subjects for dental treatments were used as material that contains the
Amelogenin gene(amel), which is the key of sex determination in our study .PCR
reaction was performed for a series of dilutions prepared from 10ng DNA to assess
the minimal amount of DNA required for PCR amplification which is the primary
concentration of the extracted DNA from dental materials of all 20 samples. The PCR
products of the 1:100 DNA dilutions which is consider the optimum concentration.
The polyacrylamide gel showed two bands with molecular weight (218bp and 212bp)
for male samples and one band with molecular weight (212bp) for female samples.
The results shown that the amel gene serves as a good marker for sex determination in
the Iraqi population and the PCR-based method was sensitive and proved to be
successful for sex determination with a complete specificity.
Key words: Gender determination, PCR, Amelogenin, Teeth.
*To whom correspondence should be addressed (E-mail:amgb772005 @yahoo.com)

الخلاصةتبرز أهمية الإعتماد على تحاليل الدنا للتعريف بالأشخاص الذين تعرضوا لحوادث مأساوية مثل الإنفجاراتعندما يكون إستعمال طرائق التحقيق الجنائي مثل التصوير الإشعاعي للأسنان أو بصمات الأصابع صعباً أومستحيلاً. تٌعد الأسنان من أهم المصادر الحيوية للحصول على الدنا لما تتميز به من قوة ومقاومة للظروفالقاسية التي ترافق الحوادث وخاصة عند تمزق وإختلاط أشلاء الضحايا. تم إستخلاص الدنا من 20 عينة لأسنان47 ومن المفترض - مصابة بالتسوس تعود لذكور وإناث خضعوا لمعالجة طبيب الأسنان تتراوح أعمارهم بين 24أن تكون عينات الدنا تحتوي على جين الأميلوجنين الذي تم إعتماده في تحديد الجنس. اُجريت التفاعلات السلسليةلبلملرة الدنا المستخلص بعد إجراء سلسلة من التخافيف لتقدير أقل كمية من التركيز المطلوب للتفاعل إذ كانالتخفيف الأمثل 1:100 بالنسبة للدنا ذات التركيز الأولي ( 10 نانوغرام). أظهرت نتائج ترحيل هلام متعددالأكريلمايد وجود حزمتين للعينات المأخوذة من ذكور بوزن جزيئي 212 و 218 قاعدة نايتروجينية على التواليبينما ظهرت حزمة واحدة مع العينات المأخوذة من إناث بوزن جزيئي 212 قاعدة نايتروجينية. بينت النتائجإمكانية إعتماد جين الأميلوجنين كمؤشر جيد في تحديد الجنس في المجتمع العراقي كما أظهرت مدى حساسيةونجاح تقنية التفاعل السلسلي لبلمرة الدنا في إجراء هذا الإختبار

Keywords

Gender determination --- PCR --- Amelogenin --- Teeth.

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