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Article
Kinetics of &#945;-amylase enzyme inhuman serum

Author: Rehab J Mohhmod
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2010 Volume: 8 Issue: 3 Pages: 237-244
Publisher: Kerbala University جامعة كربلاء

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Abstract

Objective: The present study was conducted to study the general characterization of α-amylase (AMS) enzyme by studing of the effect of various factors which affected on the activity of the enzyme (α-amylase ) by caraway method, in control group and patients group who was studied . Method: Twenty five healthy subject were depended in this study, and indicated the normal level of the enzyme, the factors effect of the activity α-amylase enzyme are: pH, temperature, substrate concentration, incubation duration. The evolution of the factors exhibit significant effect on the activity of the enzyme, and the Vmax and Km were found 100,25 mg/dl respectively, in 20 patients with pancreatitis., the Vmax and Km were found in this group 71.42, 30mg/dl.Results: α-amylase activity was observed to be very stable enzyme and effective at pH (5- 7.8), temperature (37,40), 0.06 mg/dl substrate concentration. Vmax and Km were found 25 mg/dl,100 in healthy group but in patients group were found 30 mg/dl,71.42. AMS activity in serum patients of pancreatitis was very stable in pH 7-7.6, temperature 37C° substrate concentration. The results of serum enzyme activity measurement revealed significant (p<0.005) elevation of AMS activity in patients of pancreatitis when compared with those of the healthy subject.

أجريت هذه الدراسة لدراسة الخواص العامة لأنزيم α –أميليز α-amylase من خلال دراسة العوامل المؤثرة على فعالية الإنزيمات بصورة عامة والتي هي الدالة الحامضية pH, درجة الحرارة, وتركيز المادة الأساسن،و من خلال قياس فعاليته بطريقة كاراوي Carraway و تم اعتماد 25شخص سليم ومعرفة المدى الطبيعي لهذا الإنزيم, في مصل الانسان ومعرفة الظروف المثلى لفعاليته , حيث وجد أن لهذه العوامل تأثير واضح على فعالية أنزيم α –أميليز, كما درست فعاليات هدا الانزيم في مصل 20 مريض بالتهاب البنكرياس حيث وجد ان هناك فرق معنوي عند مقارنتهم بمجموعة الأصحاء (السيطرة) مقداره <0.005 كما وجد كل من قيمتي Vmax و Km للأنزيم والتي كانت 100و 25mg/dl في مجموعة الأصحاء بينما كانت قيمة كل من Km وVmax في مجموعة المرضى المصابين بالتهاب البنكرياس هما 30mg/dlو71.42 وهذا يدل على ان ميل الانزيم للارتباط بالمادة الأساس لمجموعة الأصحاء اكبر من ميله في مجموعة السيطرة.


Article
Diagnosis of Early Diabeticby The Uses of Salivary Amylase as a Detector in Al Suleimania Provinceat Shaheed Shawkat Hospital

Author: Raida Noori Hamid
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 3 Pages: 731 -733
Publisher: Babylon University جامعة بابل

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Abstract

Diabetes mellitus is one disease which can cause death . The objective from this work is the use of salivary amylase as the detector for the presence of diabetes.In Al Suleimania at Shaheed Shawkat hospital this small pilot study was made comparison for the amount of salivary amylase by taking saliva sample from 30 diabetic patients and 30 no diabetic as control and measure the amount of amylase to compare the results between them.We find Significant increase of salivary amylase in diabetic group in comparison with control group.This study represented a continuation for another research which use saliva in the diagnosis of diabetic rather than the uses of blood or urine. I suggest to consider these results as a base for further wide researches for samples from different cities in Iraq.


Article
Improvement of thermostable productivity α-amylase from local isolate Bacillus licheniformis H14.
تحسين انتاجية انزيم الفا –اميليز( (α-amylaseالثابت حراريا˝ من العزلة المحلية .Bacillus licheniformis H14

Authors: Subhi J. Hamza صبحي جواد حمزة --- Ghazi M . Aziz غازي منعم عزيز --- Hala M . Ali هالة مشعل علي
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2010 Volume: 7 Issue: عدد خاص بمؤتمر العلمي النسوي 1 Pages: 349-356
Publisher: Baghdad University جامعة بغداد

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Abstract

(28)Bacterial local isolates of Bacillus sp. were obtained from soil samples. Isolates were tested for thermostable alpha- amylase production on solid media; fifteen isolates were able to develop clear zone around the bacterial growth after floating the plates with iodine reagent (Lugol's solution). There were further tested in submerged culture which led to selection of Bacillus sp. H14since it was the most efficient .Microbial and biochemical tests showed that the local isolate Bacillus sp.H14was refered to the species B.licheniformis that signed as H14 was refered to the species B.licheniformis H14 .,To get ahigher yield of alpha – amylase(48.70unit/mg protein) production from the local isolate B.licheniformis H14 . This study used different mutation ways such as physical way by using the physical mutagen (ultraviolet light) and chemical way by using the chemical mutagen (nitrosoguanidine). Physical mutation results showed that the local isolate B.licheniformis HM14 get higher yield of alpha – amylase production(102.10 unit/mg protein) according to killing percentage (90%) while the chemical mutation results showed that the local isolate B.licheniformis HM4 get higher yield of alpha –amylase production(100.94 unit/mg protein) from the two mutant local isolates (HM14 and HM4)were the best carbon source starch (1.5%), peptone (1.5%) as nitrogen source, calcium chloride (0.02%), sodium chloride (0.05%), magnicium phosphate (0.05%), sodium di –hydrogen phosphate (0.16%), at initial pH (5) and inoculum size 1*108 cfu/ml at (50C) For (72) hours, using shaking incubator at (150) rpm.

تم الحصول على(28) عزلة من البكتريا العائدة الى جنس الـBacillusالمعزولة من التربة,واختبرت قابليتها على انتاج انزيم الفا –اميليزالثابت حراريا باستخدام الوسط الانتاجي الصلب ، وتميزت (15) عزله بقابليتها المختلفة على انتاج الانزيم وذلك بتكوينها هالة شفافة حول المستعمرة البكتيرية بعد تغطية الطبق بمحلول اليود (كاشف لوكال ), ثم انتخبت العزلة المحليةBacillus sp.H14 كونها الاغزر انتاجا للانزيم بفعاليه نوعيه (48.70وحدة /ملغم بروتين) باستخدام المزارع المغمورة واظهرت فحوص التشخيص ان العزلة Bacillussp.H14 تعود الى البكتريا B.licheniformisورمزت لها B.licheniformis H14 .تم تحسين انتاجية الانزيم من العزلة المحلية B.licheniformis H14 باستخدام الطريقة الفيزيائية باستخدام المطفر الفيزيائي (الاشعة فوق البنفسجية (Ultraviolet light والطريقة الكيميائية باستخدام المطفر الكيميائي (النايتروزوكوانيدين (Nitrosoguanidine.اظهرت نتائج التطفير الفيزيائي بالاشعة فوق البنفسجية واعتماد نسبه القتل 90% تميز العزلة المحلية الطافرة B.licheniformis HM14 بانتاجها العالي للالفا- اميليز بفعالية نوعية (102.10وحدة /ملغم بروتين )كذلك اظهرت نتائج التطفير الكيميائي بالنايتروزوكوانيدين واعتماد نسبة القتل 90% تميز العزلة المحلية الطافرة B.licheniformis HM4 بانتاجها العالي للالفا –اميليزبفعالية نوعية (100.94وحدة /ملغم بروتين ) . حددت الظروف المثلى لانتاج الانزيم بطريقة المزارع المغمورة لكلا العزلتين الطافرتين B.licheniformis HM14و4 B.licheniformis HMبإستخدام النشأ مصدراً كاربونياً 1.5% ، و الببتون مصدرًا نايتروجينياً بتركيز1.5 % ، و كلوريد الكالسيوم 0.02% ، و كلوريد الصوديوم 0.05% ، و فوسفات المغنيسوم 0.05% و فوسفات الصوديوم ثنائية الهيدروجين 0.16% على التوالي ، و لقح الوسط الانتاجي بعدد خلايا 1×108 خلية / مليليتر عند رقم هيدروجيني ابتدائي مقداره 5 بعد 72 ساعة من الحضن بدرجة حرارة 50م بالحاضنة الهزازة بسرعة 150 دورة/دقيقة.


Article
Alpha Amylase Production by Aspergillus Oryzae Using Solid State Fermentation

Author: Suhad Adnan Ahmed
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2011 Volume: 29 Issue: 14 Pages: 2954-2960
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

A fungal strain of Aspergillus oryzae was used for the production of alpha amylase by solid state fermentation from agro-industrial waste. Enzyme production was growth associated and maximum activity (8.23 U/ml) were obtained after 120h when incubated at 30°C on wheat bran with initial moisture content 60%; initial medium pH = 5 . Enzyme activity increased when the solid medium was supplemented with additional nitrogen source (sodium Nitrate).


Article
EFFECT OF PARACETAMOL ON SALIVA α-AMYLASE
تأثير مادة الباراسيتامول على أنزيم α- أميليز اللعابي

Author: Othman R. Hameed Alsamarrai عثمان رشيد حميد السامرائي
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2011 Issue: 2 Pages: 257-263
Publisher: Kerbala University جامعة كربلاء

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Abstract

The effect of paracetamol on the activity of saliva α-amylase was carried out. We prepared three different concentrations solutions of paracetamol (300, 500, 750) mg/100ml. The effect of different volumes (5, 10, 20, 30, 50) µl of paracetamol for each concentration were study. The results showed that paracetamol has inhibition effect on the activity of α-amylase enzyme and highest inhibition at 10µl of inhibitor, while the degree of inhibition for three different concentrations were (52.30, 54.34, 20.70) % respectively. Also, kinetic of enzyme showed km value 1.428 mmol/L, and Vmax value 30303.03 mol.liter-1.min-1, the result showed that inhibition is uncompetitive type.

تناول البحث دراسة تأثير الباراسيتامول بشكله النقي على فعالية α­ أميليز اللعابي، حيث تم تحضير ثلاثة محاليل مختلفة التركيِز من مادة الباراسيتامول وهي )300، 500، 750( ملغم/100مل ودرس تأثيرها على نشاط الأنزيم بإضافة حجوم مختلفة من المحاليل الثلاثة وهي )5،10،20،30،50( مايكرو لتر وأظهرت النتائج ان للباراسيتامول تأثير تثبيطي على نشاط الأنزيم وان أعلى تثبيط عندما يكون حجم المثبط مماثل لحجم اللعاب وهو 10 مايكرو لتر وكانت درجة التثبيط للتراكيز الثلاثة هي )52.30، 54.34، 20.70(% على التوالي، كذلك تم دراسة حركية الأنزيم وكانت قيمة Km 1.428 ملي مول/ لتر أما قيمة Vmax فكانت 30303.03 مول/ لتر دقيقة وقد أظهرت النتائج ان التثبيط هو من النوع اللاتنافسي.


Article
Effect of SiO2 particles on the biodegradability of starch/PVA blends
تأثير دقائق SiO2 على التفكك الاحيائي لخلائط Starch /PVA

Authors: Nahida J. H. ناهده جمعه المشهداني --- Amnah S. A. امنه سلام عبد الرحمن
Journal: Iraqi Journal of Physics المجلة العراقية للفيزياء ISSN: 20704003 Year: 2018 Volume: 16 Issue: 37 Pages: 52-64
Publisher: Baghdad University جامعة بغداد

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Abstract

The present work aimed to study the SiO2µPs, and NPs effect on the biodegradability of St/PVA blends. The samples were prepared by casting method as PVA, St/PVA blends with different concentrations (30, 40, 50, and 60 %). FTIR test was carried out for the samples preparation. The results proved some changes which might be related to changing in crystallinity of St/PVA matrix as well as physical incorporation of SiO2 µPs, and NPs addition. The enzymatic test and water uptake results proved that increase in weight loss with increases of starch ratio. The lowest weight loss was PVA; the highest weight loss is 60% St/PVA whereas the lowest weight loss is 30%St/PVA for blends involved. SiO2µPs (753.7 nm), and NPs (263.1 nm) were added at different concentrations (1.5, 2, and 2.5 %). 1.5% SiO2 µPs, and NPs were the lowest weight loss then it was increased by SiO2µPs, and NPs addition. The samples were investigated with optical microscope. It was concluded that the samples involved could be used as packaging materials for medical application and its degradation could be controlled by SiO2µPs, and NPs addition.

يهدف هذا البحث الى دراسه تاثير NPs و SiO2µPs على التفكك الاحيائي لخلائط St/PVA. حضرت النماذج بطريقه الاذابه كنماذج من PVAوخلائط St/PVA بمختلف التراكيز (30, 40, 50, 60 %) .اجري فحصFTIR على النماذج مدار البحث .اظهرت النتائج بعض التغيرات ذات العلاقة بتغير البلوريه في اساس starch/PVA اضافه الى المساهمه الفيزياويه الناجمه عن اضافه NPs و SiO2µPs. اظهرت نتائج الفحوصات الانزيميه و الامتصاصية زياده في الامتصاصيه و الفقدان بالوزن بزيادة نسبه النشا. ان اقل امتصاصيه وفقدان بالوزن كانت ل PVA, في حين اعلى امتصاصيه و فقدان بالوزن كانت ل60%St/PVA و اقل امتصاصيه و فقدان بالوزن كانت ل 30%St/PVA بالنسبه الخلائط مدار البحث. تم اضافتNPs (263.1 nm) و SiO2µPs (753.7 nm) بمختلف التراكيز (%(1.5, 2, 2.5 . حيث ان NPs وSiO2µPs 1.5 اعطى اقل امتصاصيه و فقدان بالوزن ثم ازداد بزيادة نسبة NPs و SiO2µPs. فسرت النتائج باستخدام المجهر الضوئي .نستنتج بان نتائج مدار البحث يمكن استخدامها كمواد تعبئه وتغليف للتطبيقات الزراعيه و الطبيه ويمكن السيطره على التفكك من خلال اضافه NPs و.SiO2µPs

Keywords

PVA --- corn starch --- SiO2 --- biodegradability --- α-amylase.


Article
Inhibitory effect of gundelia extract on urinary α-amylase activity of type-I diabetes mellitus
التأثير المثبط لمستخلص الجونديليا على نشاط البول ألفا-الأميليز لمرض السكري من النوع الأول

Authors: Hamid G. Hasan --- Namam S. Hamad
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2010 Volume: 14 Issue: 3 Pages: 1-6
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: α-amylase is an enzyme that degrades starch into maltose and glucose by hydrolyzing α-1,4-glucan bonds.It is known that the enzyme is found to be elevated in patients suffering from diabetes mellitus.A potent extracts of locally distributed wild plant,the Gundelia was found to inhibit elevated activity of α-amylase with dose responses.Methods: Samples of 50 diabetes mellitus type-I has been investigated for urinary α-amylase activity.An inhibitory dose of Gundelia extract were used for the inhibition of the elevated enzyme activity.The data obtained were compared with that of healthy controlled samples﴾30﴿.The adopted protocol was a colorimetric determination﴾Anonymous,1980﴿ .Results: α-amylase activity was found to be elevated﴾281.70±10.03 IU/24hr) in patient’s serum compared with that of controlled samples﴾43.38±3.33 IU/24hr﴿.The activity of the enzyme was found to be inhibited﴾160.11±250.4 IU/hr﴿ in patients serum using dose response of 15mg/ml of Gundelia extract. The study were comprehensive to determined the physical parameters of the enzyme activity and a values of Vmax and Km were obtained.Conclusion: It was known that Gundelia is used in prevention and treatment of liver diseases. The plant has a role in the body as an antioxidant factor.It has a hypolipemic effect, therefore, a use of such plant extract could have a hypoglycemic activities on patients with DM-I.


Article
Alpha – amylase enzyme evaluation In Saliva of acutely stressed student

Author: Assist Prof. Dr. Hayder Hamed Abed. * د.حيدر حميد
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2012 Volume: 9 Issue: 1 Pages: 89-93
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Alpha - amylase is one of the major protein components of saliva. The mainfunction of salivary alpha-amylase is the enzymatic digestion of carbohydrates, but itis also important for mucosal immunity in the oral cavity. The salivary enzyme alphaamylasehas been proposed as a marker for stress-induced activity of the sympatheticnervous system. In the growing field of amylase research, recent studies haveunderscored the usefulness of salivary alpha-amylase in this regard.Fifteen Iraqi male students were considered in this study with mean age of 19.0years old during examination period as stress students, another fifteen studentswithout examination were considered as .control. α-Amylase assay were achieved byusing colorimetric photometry method.The collected data predicted elevation of amylase level from 2354.260 ± 379.757to 2817.977 ± 125.375 IU/L with a significant change. Positive correlationwas recorded when plotted the correlations values of stress and no stress individual's.In Conclusion we found that acute stress caused by examination associated withelevation of amylase in saliva significantly, however one of the main causes ofacademic stress is test anxiety for Undergraduate students seem to be moreemotionally vulnerable due to examinations.


Article
Relation of ل-Amylase Activity with Glucose and Anti-Gliadin IgA and IgG in Sera of Patients with Celiac Disease

Authors: Dahlia M.R. Al-Abadi --- Aliaa H. Faraj --- Sura A. Al-Emami
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2011 Volume: 20 Issue: 1 Pages: 81-86
Publisher: Baghdad University جامعة بغداد

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Abstract

Celiac disease (CD) is an inflammatory small intestinal disorder that can lead to severe villous atrophy, and malabsorption . Since the measurement of ل-amylase activity is the most widely used biochemical test for the diagnosis of pancreatic and non pancreatic disease , therefore serum ل-amylase were studied in the present study in an attempt to evaluate the usefulness of this enzyme in the diagnosis of celiac disease and its relationship with anti gliadin IgA and IgG and serum glucose . Thirty one patients with celiac disease were studied and compared with twenty four healthy individuals . Significant elevation of ل-amylase activity , glucose and anti gliadin IgA and IgG were observed in the sera of patients with celiac disease compared with the control group . Also a significant positive correlation between ل-amylase activity and anti gliadin IgG were found in the present study in the sera of patients with celiac disease while a non significant correlation were found between ل-amylase activity and anti gliadin IgA and glucose in the sera of the same patients of celiac disease.

داء الاحتشاء هو التهاب يحدث نتيجة خلل معوي صغير ٬ من الممكن ان يؤدي إلى حدوث ضمور زغابي شديد وسوءامتصاص . ولكون قياس فعالية الفا – اميليز يعد من أكثر الفحوص الكيميائية الحياتية استخداما لتشخيص الأمراض البنكرياسية وغيرالبنكرياسية , لذا فقد تم قياس هذا الأنزيم في الدراسة الحالية في محاولة لتقدير أمكانية استخدام الفااميليزفي تشخيص مرض الاحتشاء٬ حيث تضمنت الدراسة الحالية جمع 31 عينة من مرضى المصابين بداء IgG , IgA وعلاقته بكلكوز مصل الدم ومضادات الكلايدنالاحتشاء ومقارنتها ب 24 عينة من الأشخاص الأصحاء . أظهرت النتائج وجود زيادة معنوية في فعالية الفا اميليزومستوى كل منفي مصول دم المصابين بداء الاحتشاء مقارنة مع مستوياتها في مصول دم الأصحاء وقد تبين IgG , IgA الكلكوز ومضادات الكلايدنفي مصول دم المصابين بداء الاحتشاء في حين تبين عدم وجود IgG أيضا وجود علاقة ايجابية معنوية بين فعالية الفااميليزومضادفي مصول دم المصابين بداء الاحتشاء . IgA علاقة معنوية بين فعالية الفااميليزوكل من الكلكوز ومضاد


Article
Effects of diabetes mellitus types II on salivary flow rate and some salivary parameters (total protein, glucose, and amylase) in Erbil city

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Abstract

Background: The concentration of some components of saliva may be associated with certain systemic illnesses,reflecting the hormonal, immunological, neurological, emotional, nutritional and metabolic states of the patient. Theaim of this work was to assess salivary flow rate, and to evaluate saliva samples for levels of salivary total protein,glucose, and alpha amylase, in diabetics type II and healthy subject in both genders.Subjects and methods: Unstimulated salivary flow rate, salivary total protein, glucose, and amylase were measuredin 90 subjects, 60 with diabetes mellitus type II (30 controlled and 30 uncontrolled diabetic patients) and in 30 healthysubjects.Results: Significant difference in salivary flow rate in diabetic patient when compared with healthy subject. Thefinding showed no significant differences between salivary total proteins in all groups. Significant difference in salivaryglucose and amylase concentration was found between the healthy subjects and type 2 diabetic patients.According to the gender, there were only significant differences between male and female in salivary flow rate forhealthy subjectsConclusions: Patients with type 2 diabetes mellitus have higher concentration of salivary glucose and lower value ofsalivary flow rate and amylase. No significant difference was seen in protein value in all groups

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