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Article
Evaluation of Performance Characteristics of Commercially Available Tests for Diagnosing Hepatitis B Surface Antigenemia

Authors: Saad Hasan Mohammed Ali --- Saja Jehad Al-Khalidi --- Waleed Nadi Kasim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 159-171
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:A number of serologic immunoassays techniques have been developed in diagnostic virology with different degrees of sensitivity and specificity for the detecting hepatitis B virus (HBV) antigens and their relevant antibodies.OBJECTIVE:This study was designed to apply and assess the sensitivity and specificity of different commercially available laboratory techniques for detecting hepatitis B surface antigen(HBsAg).METHODS:One hundred and twenty-one sera samples were collected from National Center for Blood transfusion, Gastroenterology and Liver Diseases Hospital, Central Public Health Laboratories and Teaching Laboratories. According to the manufacturer’s practical instructions, a group of commercially available laboratory methods for detecting HBsAg were applied , including enzyme linked immunosorbent assay (ELISA), enzyme linked fluorescent assay(ELFA), immunochromatographic assay(ICA), and latex agglutination test(LAT).RESULT:Among ELISA, ELFA, ICA, and LAT methods for detecting HBsAg, the 3rd generation ELISA was proved to have very high specificity (no false negative results) and the least one that has necessitated few confirmatory repetitions. ELFA versus ELISA has showed relatively lower sensitivity (more false negative results). However, similar to ELISA, ELFA showed very high specificity. Immunochromatographic assay (ICA) confidentially appeared to be a good, rapid and simple technique with comparable sensitivity and specificity to ELISA and ELFA techniques. Although LAT was introduced as a rapid, simple and cheap technique for HBsAg screening, it showed frank lower sensitivity and specificity that deranged it from competing with all those tested techniques.CONCLUSION:The concomitant use of ELFA with ELISA compensates its relatively lower sensitivity in front of ELISA. Latex agglutination test for HBsAg has relatively lower sensitivity and specificity than all other tests. For its comparable performance characteristics to ELISA, the use of ICA is ideally suited for HBsAg screening, in respect to its lower cost, rapidity, simplicity and no need for expensive equipments


Article
Effects of Methotrexate and Etanercept on C-Reactive Protein and Anti-Cyclic Citrullinated Peptide Antibodies level In Rheumatoid Arthritis Patients
آثار الميثوتريكسيت وetanercept وعلى بروتين سي التفاعلي ومستوى مكافحة دوري Citrullinated الببتيد الأجسام المضادة في التهاب المفاصل الروماتويدي المرضى

Authors: Khawla H. Zhair خولة زهير --- Rasha Hussain Kuba رشا حسين --- Yasser abdulhussain Jafar ياسر عبد الحسين
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2016 Issue: Special Second International Scientific Conference for the Life Sciences Pages: 120-124
Publisher: University of Kufa جامعة الكوفة

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Abstract

Rheumatoid Arthritis (RA) is an autoimmune disease. The treatment of this disease by taking Etnercept and Methotrexate (MTX) drugs. This study was conducted to detect C-Reactive Protein (C-RP) and Anti- Citrullinated Peptide Antibodies (Anti-CCP Abs) level among 126 rheumatoid arthritis (RA) patients treated with Etanercept (ETN) and methotrexate (MTX), 94 RA patients treated with MTX, 80 RA patients without treatment and 100 samples as healthy control. Blood samples were collected and the presence of C-RP antibodies was determined by using Latex agglutination test (LAT). Anti-CCP Abs was also estimated in serum of all subjects by using Enzyme linked immunosorbent assay (ELISA) method too. The seroprevalence of C-RP in RA+ETN+MTX was 57(45.24%), in RA+MTX was 62(65.96%), in untreated RA patients was 53(66.25%) while, it was 5(5%)in healthy group. The mean levels of Anti-CCP Abs in RA+ETN+MTX patients were (18.53± 1.70 pg/ml), in RA+MTX patients was (47.61± 6.24 pg/ml), and in untreated RA patients was (167.98 ± 8.67 pg/ml)while, they were (10.3 pg/ml ± 0.87) in healthy group. The results showed significant difference (P<0.05) for both C-RP prevalence and Anti-CCP Abs serum levels was found between studied subjects.

التهاب المفاصل الروماتويدي (RA) هو أحد أمراض المناعة الذاتية. علاج هذا المرض عن طريق تعاطي المخدرات Etnercept والميثوتريكسيت (MTX). وقد أجريت هذه الدراسة للكشف عن بروتين سي التفاعلي (سي آر بي) ومكافحة Citrullinated الببتيد الأجسام المضادة (مضاد للCCP عبس) مستوى بين 126 التهاب المفاصل الروماتيزمي (RA) المرضى الذين عولجوا مع etanercept و(ETN) والميثوتريكسيت (MTX)، 94 RA المرضى الذين عولجوا مع MTX، 80 مرضى التهاب المفاصل الروماتويدي من دون علاج، و 100 عينات السيطرة كما صحية. تم جمع عينات الدم وتم تحديد وجود أجسام مضادة C-RP باستخدام اختبار تراص اللاتكس (LAT). وقدرت مكافحة CCP عبس أيضا في مصل الدم من جميع المواد الدراسية باستخدام انزيم مرتبط طريقة الفحص المناعي (ELISA) أيضا. وكان الانتشار المصلي من C-RP في الاتحاد الإقليمي + ETN + MTX 57 (45.24٪)، في RA + MTX كان 62 (65.96٪)، في و53 (66.25٪)، في حين، كان مرضى التهاب المفاصل الروماتويدي غير المعالجة 5 (5٪) في مجموعة صحية. وكانت المستويات الوسيطة لمكافحة CCP عبس في الاتحاد الإقليمي + ETN + MTX المرضى (18.53 ± 1.70 جزء من الغرام / مل)، في RA + المرضى MTX كان (47.61 ± 6.24 جزء من الغرام / مل)، وفي مرضى التهاب المفاصل الروماتويدي غير المعالجة كان (167.98 ± 8.67 جزء من الغرام / مل) بينما كانت (10.3 غ / مل ± 0.87) في مجموعة صحية. أظهرت النتائج فروق معنوية (P <0.05) لكل من انتشار C-RP ومكافحة CCP عبس مستويات المصل تم العثور بين المواضيع التي شملتها الدراسة.

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