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Article
Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes

Authors: Tagreed U.Mohammd --- Saad S. Saleh**, Nadia M.Al-Mhana --- Azhaar F.Abd Al-Zhra
Journal: Journal of Engineering and Sustainable Development مجلة الهندسة والتنمية المستدامة ISSN: 25200917 Year: 2012 Volume: 16 Issue: 4 Pages: 453-461
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and serum of patients with type 2 diabetes respectively. The purified enzyme showed single peak. The results of this study revealed that AST activity of type 2 diabetes urine and serum increased significantly (p<0.001) compared with control group.

تم تنقية الأنزيم الناقل لمجموعة الأمين من ادرار وامصال المرضى المصابين بداء السكري النوع الثاني من خلال خطوتين: الاولى بأستخدام الديلزة والثانية باستخدام الترشيح بالهلام Sephadex G-25 (كروموتوغرافيا العمود). نقي الأنزيم الناقل لمجموعة الأمين AST 236.23 مرة مع الناتج 1467% و3.46 مرة مع الناتج 142.85% من ادرار وأمصال المرضى المصابين بداء السكري النوع الثاني على التوالي. أعطى الأنزيم المنقى بالترشيح الهلامي قمة واحدة. تشير نتائج هذه الدراسة الى نشاط الأنزيم AST المنقى من ادرار وامصال المرضى المصابين بداء السكري النوع الثاني أعطى تغيراً معنوياً ملحوظاً(P<0.001) مقارنة بالأصحاء.


Article
The Liver Aminotransferase Levels in Diabetes Patients

Authors: Lobna Dheyaa Jaafar --- Dheyaa Shinyar Hammad --- Taghreed Ali Obeed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 3 Pages: 797-801
Publisher: Babylon University جامعة بابل

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Abstract

This study aim toinvestigate the levels ofliver aminotransferase in patients with diabetes and the effect of glycemic control on the levels of these enzymes.The study was conducted on 115 persons, 19 poorly controlled diabetic patients (glucose>300 mg/dl), 25 moderately controlled diabetic patients (glucose 200-300 mg/dl), 21 well controlled diabetic patients (glucose < 200 mg/dl), and 50healthy non diabetic subjects as normal control. Serum glucose, ALT and ASTwere assessed in all groups.There was no statistically difference in ALT and AST levels between control and total diabetes patient groups. Poorly controlled diabetic patients demonstrated highly significantly increase of ALT and AST when compared with the other two patient groups (well and moderate controlled diabetic). But there is no significant statistic for these enzymes between well and moderate controlled diabetic groups. In conclusion,increased levels of ALT and ASTmay contributory factor to induced liver disease observed in poorly glycemic control patients.


Article
Kinetic studies of AST isoenzymes I,II,III,IV partially purified from patient,s urine with chroinc renal failure
دراسات حركية لمتناظرات أنزيم الأسبارتيت أمينو ترانسفريزAST IV,III,II,I المنقاة جزئيا من ادرار المرضى المصابين بالعجز الكلوي المزمن

Authors: Tagreed U.Al-Akabie تغريد علوم العقبي --- Sami A. AL-Mudffer سامي عبد المهدي المظفر --- Ferah Gh.Al-Salihi فراح غالي الصالحي
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2010 Volume: 7 Issue: عدد خاص بمؤتمر العلمي النسوي 1 Pages: 437-443
Publisher: Baghdad University جامعة بغداد

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Abstract

In this research, the kinetic studies of four isoenzymes of Asprtate aminotransferase, which partially purified from the urine of chronic renal failure patients were carried out .The four isoenzymes were obeyed Michaelis-Menton's equation and the optimum concentration of their substrate (Aspartic acid) was (166.5x10-3) mole/liter,and their Km values were determined. Four isoenzymesI,II,III,IV have shown an optimum pH at 7.4.The four isoenzymes obeyed Arrhenius equation up to 37º C and their Ea and Q10 constants were determined .

تضمن البحث الحالي دراسة الصفات الحركية لمتناظرات أنزيم الأسبارتيت أمينو ترانسفيريزٍِAST IوIIوIIIوIV المنقاة جزئيا من ادرار المرضى المصابين بالعجز الكلوي المزمن، أذ وجد أن المتناظرات الاربع تخضع لمعادلة ميكاليس- منتن ،حيث كان الـــتركيز الأمثل للمادة الأســـــاس (حامض الأسبارتيك) لكـــل من المتنـــاظرات IوIIو IIIو IV (166.5x10-3) مول/لتر، كما وجد حدوث أرتفاع في سرعة تفاعل المتناظرات الاربع مع أرتفاع الدالة الحامضية الى أن يصل الى سرعة التفاعل القصوى عند الأس الهيدروجيني (7.4) .وقد لوحظ أن المتناظرات تخضع لمعادلة أرينوس حتى (37)º م وتم تعيين الثوابت Ea وQ10 لكل من المتناظرات الاربع.


Article
Purification of (AST) From Sera of Type П Diabetic Patients
تنقية أنزيم اسبارتيت امينو ترانسفيريز في أمصال مرضى السكري من النوع الثاني

Author: Sayran Sattar Saleh سيران ستار صالح
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2011 Issue: 2 Pages: 35-41
Publisher: Kerbala University جامعة كربلاء

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Abstract

This study was performed on (35) serum specimen of patients having type 2 diabetes in addition , (40) normal specimens were investigated as control group . The activity of (AST) in diabetic patients was reached to (75.2 ± 11.7) IU/L as compared with normals (19.9 ± 6.1) IU/L . Purification of (AST) from sera of diabetic patients was performed by dialysis and gel filteration (Sephadex G 25) the results of study reveal that Aspartate aminotransferase (AST) activity of type 2 diabetes patient's serum show ahigh significant increase (p< 0.001) compare to normal subject.

شملت الدراسة ( 35 ) عينة مرضية من أمصال المرضى المصابين بالداء السكري النوع الثاني فضلا عن ( 40 ) عينة من أمصال الاصحاء كمجموعة ضابطة . وقد أظهرت النتائج ارتفاع معنوي بنشاط الانزيم (AST) في أمصال المرضى المصابين بالداء السكري النوع الثاني مقارنة بالاصحاء . كما تم تنقية الانزيم باستعمال كيس الديلزة وكروموتوغرافيا الترشيح يالهلام (Sephadex G – 25)


Article
Correlation of New Vitamin C Derivatives with Alanine Amino Transferase and Aspartate Amino Transferase Activities

Authors: Mufeed J.Ewadh --- Seenaa Badr --- Ibtesam H. Al Musawi --- Hamid G. Hasan --- et al.
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 2 Pages: 285-291
Publisher: Babylon University جامعة بابل

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Abstract

Background: Liver enzymes are commonly found in liver cells and when the liver is damaged, liver cells release their enzymes into the blood stream. Vitamin C is a low-weight molecular antioxidant and is effective in the aqueous phase in protecting different parts of cells against free radicals. An enzyme inhibitor is a molecule, which binds to enzymes and affect their activity.Objective: This study was carried out to investigate the effect of new vitamin C derivative on serum liver enzymes.Research design and methods: Eight samples were used. Liver enzymes activity measured with and without vitamin C derivative. Results: The result showed a reduced activity of aspartate aminotransferase (AST), and alanine transferase (ALT) after addition of vitamin C derivative.Conclusion: It is concluded that the decrease in ALT, and AST activities were due to noncompetitive inhibition of enzymes by vitamin C derivative.

أنزيمات الكبد موجودة عادة في خلايا الكبد وعند تلف الكبد يتم الإفراج عن الانزيمات الخاصة في مجرى الدم. فيتامين C هو منخفض الوزن الجزيئي للأكسدة وفعال في المرحلة المائية في حماية أجزاء مختلفة من الخلايا ضد الجذور الحرة. مثبط الإنزيم هو جزيء، الذي يرتبط بالانزيمات ويؤثر على نشاطهم. أجريت هذه الدراسة لمعرفة تأثيرمشتق فيتامين C الجديد على انزيمات الكبد في الدم. استخدمت ثمانية عينات. النشاط قياس انزيمات الكبد بدون ومع فيتامين C المشتق. أظهرت النتيجة انخفاض نشاط ASTو ALT بعد إضافة فيتامين C المشتق. ويستنتج من ذلك أن الانخفاض في ALT، AST نتيجة التثبيط غير التنافسي على الانزيمات الذي اظهره مشتق فيتامين C.


Article
Determination of the Liver Enzymes Level and Kidney Functions in Pregnant Women in the Second Trimester of Pregnancy at Different Age Groups
تعيين مستوى انزيمات الكبد ووظائف الكلى في النساء الحوامل في الثلث الثاني من الحمل بمجاميع عمرية مختلفة

Author: Suha A. Al-Jowary سهى عبد الخالق الجواري
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 4A Pages: 1802-1807
Publisher: Baghdad University جامعة بغداد

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Abstract

The level of liver enzymes and kidney functions in pregnant women in the second trimester of pregnancy at different age groups was determined. This study is composed of fifty pregnant women in the second trimester of pregnancy and were classified into two subgroups; first group included twenty-five pregnant with an age between 22-30 years, a second group included twenty-five pregnant with an age 35-42 years. A control group included twenty-five non-pregnant, healthy women was also included. Blood samples were obtained from each group, centrifuged, serum was collected from each group to measure liver enzymes (AST, ALT and ALP) and kidney function tests (urea and creatinine) were measured using enzymatic kits. The results of present study illustrated significant elevation (P≤ 0.05) in AST and ALT means in the age group 35- 42 years, while there were no-significant differences in an age group 22- 30 compared with control healthy group. In addition, the ALP mean values are significantly increase(P≤0.05) in 22- 30 and 35- 42 years compared with non-pregnant women. The findings also demonstratedthat the means of blood urea is significantly declined (P≤0.05) in the age groups 22- 30 and 35- 42 years compared with healthy non-pregnant. This studyfoundsignificant decline(P≤0.05) in serum creatinine at an age group 22- 30 and significant increase(P≤0.05) in the other group (35- 42 years) compared with the control. The conclusion of the present study that hepatic and renal functions are more damaged in an age group 33- 42 years than an age group 22- 30 years through the values of parameters above. Therefore, pregnancy in the age 22- 30 years is more healthyfor both mother and fetus than an age 33- 42 years.

تم دراسة مستوى انزيمات الكبد ووظائف الكلى في النساء الحوامل في الثلث الثاني من الحمل بأعمار مختلفة, تضمنت هذه الدراسة خمسين امرأة حامل في الثلث الثاني من الحمل وصنفت الى مجموعتين ثانويتين: المجموعة الاولى تكونت من 25 حامل بعمر من 22- 30 سنة, اما المجموعة الثانية فإنها تكونت ايضا من 25 حامل بعمر من 35- 42 سنة, ومجموعة السيطرة فقد تكونت من 25امراة غير حامل اصحاء, جمع مصل من كل المجاميع لقياس مستوى انزيمات الكبد ووظائف الكلى بالعدد الانزيمية الخاصة. اشارت نتائج الدراسة الحالية ارتفاعا معنويا(P≤0.05) في معدلات انزيمي الاسبارتيت الناقل للأمين والالنين الناقل للأمين بعمر35- 42 سنة ولم تظهر فروقات معنوية بعمر 22- 30 سنة مقارنة مع مجموعة السيطرة الاصحاء, بالإضافة لذلك فان معدلات الفوسفاتيز القاعدي قد ارتفعت (P≤0.05) 0.05)في مجموعتي 22- 30 و35- 42 سنة مقارنة مع الاصحاء غير الحوامل, لقد اوضحت النتائج ايضا بان معدلات يوريا الدم قد انخفضت معنويا(P≤0.05) بعمر 22- 30 وبعمر 35- 42 سنة مقارنة مع النساء غير الحوامل الاصحاء,اظهرت هذه الدراسة اظهرت انخفاضا معنويا(P≤0.05) بعمر 22- 30 سنة وارتفاعا معنويا في المجموعة الاخرى (35- 42 سنة) مقارنة مع مجموعة السيطرة. يستنتج من الدراسة الحالية بان وظائف الكبد والكلى اكثر تضررا بعمر 33- 42 سنة مما بعمر 22- 30 سنة من خلال قيم المتغيرات اعلاه, لذلك فان الحمل بعمر 22- 30 سنة اكثر صحيا للام والجنين من الحمل بعمر 33- 42 سنة.


Article
The Value of Free Thyroid Hormones and Aspartate Aminotransferase in Vaginal Washing Fluid for Detection of Preterm Pre-Labor Rupture of Membrane

Author: Maha Mohamed Jasim Al-Bayati*, Eaman Marouf Muhammad**, Athmar Maamon Salman**
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2019 Volume: 18 Issue: 1 Pages: 52-58
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Preterm pre-labor rupture of membrane occurs in about 2% of all pregnancies. The most frequentconsequences of preterm pre-labor rupture of membrane is preterm delivery, sepsis and pulmonaryhypoplasia. The correct diagnosis of preterm pre-labor rupture of membrane is crucial and successfulmanagement will be based upon it.OBJECTIVE:To determine whether measurement of vaginal washing fluid aspartate aminotransferase(AST) ,free triiodothyronine (free T3) and thyroxin(free T4) were useful tests for the diagnosis of pretermpre-labor rupture of membrane.STUDY DESIGN: A case - control study.SETTING: :This study was conducted at department of Obstetrics and Gynecology of AL-Yarmoukteaching Hospital and National center for DiabetesPATIENTS, MATERIALS AND METHODS:It included hundred pregnant women with a gestational age ranging between 24 - 36+6 weeks. Theywere divided into two groups, the study group which included fifty pregnant women presented withpreterm pre-labor rupture of membrane and the control group which includes fifty pregnant womenwithout any complaint, matched for gestational age. All women underwent sterile speculum vaginalexamination. Free triiodothyronine and free thyroxin levels were measured by using Enzyme immuneassay method, and aspartate aminotransferase level was measured by using colorimetric method, invaginal washing fluid.RESULTS:Vaginal washing fluid free T3, free T4 and AST levels were significantly higher in the study group(1.15), (0.071), (4.9) respectively compared with the control group (0.93), (0.048), (4.5) respectively.Vaginal washing fluid free T4 having the largest area under the curve on receiver operatingcharacteristic curve (ROC) analysis (P<0.001). ROC curve analysis showed that free T3 validityresults in predicting preterm pre labor rupture of membrane among pregnant women were (cut-off1.07pg/ml, sensitivity 88%, specificity 76% and accuracy 82%), free T4 validity results were (cutoff0.055pg/ml, sensitivity 84%, specificity 80% and accuracy 82%) and AST validity resultswere (cut-off 4.9IU/L, sensitivity 52%, specificity 80% and accuracy 66%).CONCLUSION:Measurement of aspartate aminotransferase free triiodothyronine , and thyroxin in vaginal washingfluid of suspected and diagnosed patient presented with preterm pre labor rupture of membranefound to be a useful markers for the diagnosis of preterm pre labor rupture of membrane..


Article
Assessment of Liver Enzymes Activity in Patients with Rheumatoid Arthritis in Nineveh province

Author: Hiba R. Tawfeeq
Journal: Tikret Journal of Pharmaceutical Sciences مجلة تكريت للعلوم الصيدلانية ISSN: 18172716 Year: 2012 Volume: 8 Issue: 2 Pages: 284-290
Publisher: Tikrit University جامعة تكريت

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Abstract

Aim of the study: to determine the effect of Rheumatoid Arthritis(RA) on liver enzymes level. Methods: 75 subjects were enrolled in this study (40 patients with Rheumatoid arthritis (RA) and 35 healthy subjects as control group), at Mosul city, Northern of Iraq, aged 20-70 years. The biochemical parameters measured were some of liver function tests which include measurement of the liver enzymes activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT). Results: There was a significant increase in liver enzymes activity in patients when compared to control. There was also significant increase in serum AST level at age group 40-50 years when compared to other age group in patients, in addition, there was a significant increase in serum ALP in female patients when compared to male patients. There was non-significant change in liver enzymes activity when compared according to residence, according to family history, and according to rheumatoid factor in patients. Conclusion: The results of the present study indicated no deterioration in liver functions in patients with rheumatoid arthritis regarding serum enzymes (aspartate transaminase, alanin transaminase, gamma glutamyl transferase and alkaline phosphatase), although subclinical alteration in liver functions could be expected in those patients, so that measurement of other early markers of liver dysfunction is occasionally recommended.

هدف البحث: تحديد تأثير مرض التهاب المفاصل الرثواني على مستوى انزيمات الكبد. طرق البحث :75 شخص شملوا في هذه الدراسة 40من المصابين بالتهاب المفاصل الرثواني و35شخص كمجموعة ضبط , في مدينة الموصل وضواحيها, شمال العراق, تراوحت اعمارهم بين 20-70سنة, تم اجراء بعض فحوصات وظائف الكبد عليهم والتي شملت قياس فعالية انزيمات الكبد والمتضمنة مستوى خميرة ناقلة امين الأسبارتات (AST), ناقلة امين الالنين (ALT),الفوسفاتيز القلوية (ALP) وناقل الغاما غلوتاميل.(GGT) النتائج: أظهرت الدراسة زيادة معنوية في معدل مستويات انزيمات الكبد(ناقلة امين الأسبارتات, ناقلة امين الالنين, الفوسفاتيز القلوية وناقل الغاما غلوتاميل في مصل الدم لدى المرضى عند مقارنتهم بمجموعة الضبط بالإضافة إلى زيادة معنوية في معدل مستوى ناقلة امين الأسبارتات عند المرضى الذين تتراوح اعمارهم بين 40-50 سنة وكذلك كان هناك زيادة معنوية في معدل مستوى الفوسفاتيز القلوية لدى المرضى الاناث عند مقارنتهم بالمرضى الذكور. كما أظهرت عدم وجود اختلافات معنوية في معدلات مستويات انزيمات الكبد لدى المرضى عند مقارنتهم طبقا" للسكن, وجود المرض في العائلة, وطبقا" لوجود العامل الرثوي في العائلة. الخلاصة: تبين نتائج الدراسة الحالية عدم وجود اضطرابات ظاهرية في وظائف الكبد لدى مرضى التهاب المفاصل الرثواني بالاعتماد على مستويات انزيمات الكبد في مصل الدم . مع ذلك فانه من المتوقع وحسب النتائج، وجود تغييرات طفيفة وغير ظاهرة سريريا" في وظائف الكبد لدى هؤلاء المرضى، وبذلك ينصح بمتابعة وظائف الكبد لدى مرضى التهاب المفاصل الرثواني عن طريق إجراء فحوصات اشمل وأدق لوظائف الكبد.


Article
Biochemical Changes of Liver Enzymes; ALT, AST, ALP in Patients after Surgical Operation Under General Anesthesia

Authors: Mohammed Abed Kadhim Al-Saadi --- Mufeed Jalil Ewadh --- Ahmed Ibraheem Rashid
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 4 Pages: 1191-1197
Publisher: Babylon University جامعة بابل

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Abstract

The physiologic state induced by general anesthetics typically includes analgesia, amnesia, loss of consciousness, inhibition of sensory and autonomic reflexes, and skeletal muscle relaxation. The liver function is mainly homeostasis. Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) are soluble cytoplasmic and mitochondrial enzymes widely used to assess hepatocellular damage. ALP Alkaline Phosphatase is membrane associated enzyme anchored to cell membrane in particular to the biliary canaliculus. ALP is found in most tissues but is derived predominantly from hepatic cells.Aim of this study was to evaluation of liver enzymes (ALP, ALT, AST ) changes that accompany surgical operation under general anesthetic drugs.A total of (36) patients under go surgical operations under general anesthesia.Blood samples were collected from patients just before and 24 hours after operation. The serum has been stored by deep freezing (-20°C) until used for serological tests to estimate the concentration of serum ALT, AST, ALP enzymes level.The results were significant increase in mean differences of serum ALT, AST, ALP enzymes level.In conclusion this study showed that surgery and general anesthesia elevated serum level of ALT, AST, ALP enzymes.


Article
Acute Toxicity Study of Indomethacin and Oxytetracycline in Rabbits

Author: Rebin Kanabi Majeed, Hawraz Farhad Muhammed1, Harseen Mahmud Rahim
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 3 Pages: 218-221
Publisher: Babylon University جامعة بابل

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Abstract

Background: The toxic effect of nonsteroidal anti‑inflammatory drugs (NASIDs) and broad‑spectrum antibiotics in human and animal sciencehas been studied widely. The advantages and the adverse impact of these medications could be determined by measuring some biochemicalparameters such as the level of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and bloodurea nitrogen (BUN). In addition, the changes in some hematoimmunological parameters could also be measured; for instance, red bloodcell (RBC) count, hemoglobin level, white blood cell (WBC) count, and differential WBC count. Objectives: The aim of this study is toevaluate the effect of both indomethacin, as a NSAID example, and oxytetracycline, as a broad‑spectrum antibiotic separately and together,on some biochemical and hematological parameters. Materials and Methods: Sixteen male albino rabbits were used, with age about 1 yearand body weight about 1.5 kg. They were divided into four groups and each group contains four rabbits. The first group served as a controlgroup, which receives only food and water. One of the groups were administered with oxytetracycline at a dose of 50 mg/kg. The secondwas administered with indomethacin at a dose of 5 mg/kg, and the final group was administered with both oxytetracycline and indomethacinwith the same previous dose. All the drugs were dissolved in the distilled water before administration of dose to each animal in the grouporally using a stomach cannula for 14 days. The rabbits were kept for 14 days under the room temperature with free access to food andwater. Serum was separated from the clotted blood obtained by ear vein. Total RBC and WBC counts were made by the hemocytometermethod. The differential WBC counts were made by finding the percentage average of the different types of cells counted in ten fields fromGiemsa‑stained slides. Serum enzymes such as ALP, AST, and ALT were determined by procedures of Sigma Diagnostics, BUN by method ofCrocker. Results: The level of ALP has shown great increase in all groups, while the level of AST and ALT was increased only in the groupthat administered with indomethacin. However, both parameters showed a significant decrease in the other groups. Regarding hematologicalchanges, WBC increased in all groups, while RBC and Hb level showed sharp decrease. Lymphocytopenia occurred in only the group thatadministered with oxytetracycline. Conclusion: The results showed toxic effect of these two most popular using drugs in animals specificallywhen it is used chronically. In addition, there is no great difference when it is used separate or together. Further study is required to investigatethe level of damage that happens in the organs.

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