research centers


Search results: Found 28

Listing 1 - 10 of 28 << page
of 3
>>
Sort by

Article
Cytogenetic Study of Azoospermia and Sever Oligospermia in Al-Anbar Governorate
دراسة وراثية ـ خلوية للأشخاص الذين يعانون من حالة اللانطفية وقلة النطف الشديدة في محافظة الانبار

Author: Samir M. Khalaf سمير مشرف خلف
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2011 Volume: 16 Issue: 2 Pages: 139-146
Publisher: Tikrit University جامعة تكريت

Loading...
Loading...
Abstract

Seminal fluid analysis was carried out for 309 samples of subfertile men from Al-Anbar Governorate. Samples were arranged in two groups according to the type of subfertility ; the first group consisted of 217 azoospermia patients (70.23%), while the second group included 92 oligozoospermia patients(29.77%) , in addition to 40 fertile men as control group. This project also included the study of pedigree analysis for all subfertile men to determine the pattern of inheritance. Hormonal tests (Lotine Hormone ,Follicle Stimulation Hormone ,Prolactine Hormone ,Testosterone Hormone) were carried to eliminate the abnormal patients. Cytogenetic analyses for 61 samples of peripheral blood lymphocytes were performed on 43 subfertile men(azoospermia) and 18 (sever oligospermia), in comparison with 40 cases of fertile men.The results showed the presence of familial continuity with genetic familial relationship in the studied samples. the frequency of families with positive family history were 16 (7.37%) and 3 (3.26%) in azoospermia and oligozoospermia patients, respectively . The study showed the presence of hormonal disorders in 248(80.26%) cases of subfertility, so they were eliminated form the study. The Cytogenetic study of peripheral blood lymphocytes for subfertile men showed the presence of some chromosomal aberrations in(16.28%) and (5.56%)of azoospermia and oligozoospermia patients respectively. However, chromosomal aberrations in the control group were not detected.

اجري تحليل السائل المنوي لـ(309) فرد من المرضى قليلي الخصوبة في محافظة الانبار والذين تم تقسيمهم إلى مجموعتين استنادا إلى نوع الخصوبة إذ تضمنت المجموعة الأولى حالات اللانطفية وعددهم217(70.23%) والمجموعة الثانية حالات قلة النطف الشديدة وعددهم 92 (29.77% )، بالإضافة إلى 40 فردا سليما وله ذرية سليمة كعينة مقارنة .تم تحليل سجلات النسب لأفراد العينة لغرض تحديد نمط توارث الإصابة بقلة الخصوبة لديهم ، بالإضافة الى إجراء بعض الفحوصات الهرمونية (Lotine Hormone ,Follicle Stimulation Hormone ,Prolactine Hormone ,Testosterone Hormone)لاستبعاد العينات التي تحوي خلل هرموني، وشملت الدراسة فحوص الوراثة الخلوية(61 حالة) منها (43 حالة) لمرضى اللانطفية و(18حالة)لمرضى قلة الخصوبة مقارنة مع (40 حالة )من مجموعة السيطرة.أظهرت نتائج دراسة تحليل سجلات النسب أن هناك سريان عائلي مع وجود علاقة عائلية وراثية لقلة الخصوبة إذ بلغت نسبة الحالات ذات التاريخ العائلي الموجب16 (7.37%) و3 (3.26%) لحالات اللانطفية وقلة النطف على التوالي.أوضحت الدراسة وجود اضطرابات هرمونية في 248(80.26%) حالة من اللانطفية ولحالة قلة النطف الشديدة وتم استبعاد هذه العينات لحصر الحالات السليمة وإجراء الفحوصات الوراثية عليها .أظهرت تحليلات الوراثة الخلوية للدم المحيطي لحالات قلة الخصوبة(اللانطفية، قلة النطف) وجود تشوهات كروموسومية بنسبة مئوية ( 16.28% )لدى المرضى المصابين باللانطفية و (5.56%) لدى المرضى المصابين بقلة النطف الشديدة ، بينما لم تلاحظ تشوهات كروموسومية عددية أو تركيبية بشكل واضح لدى عينة السيطرة.


Article
Assessment of spermatogenesis by fine needle aspiration of testes in patients with azoospermia
تقييم تكوين النطف بواسطة الرشف بالإبرة الدقيقة في المرضى المصابين بانعدام النطف

Author: Ibrahim K. S. قاسم سعيد ابراهيم
Journal: Annals of the College of Medicine Mosul مجلة طب الموصل ISSN: 00271446 23096217 Year: 2013 Volume: 39 Issue: 2 Pages: 143-146
Publisher: Mosul University جامعة الموصل

Loading...
Loading...
Abstract

ABSTRACTBackground and objectives: Recent advances of Invitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI) combined with the progress in sperm retrieval from testes have provided a hope for azoospermic patients to become fathers. The aim of this study is to describe the cytological features of the cells aspirated from testes of patients with azoospermia and to select those who have mature spermatozoa in their aspirate for assisted fertilization.Design and setting: A descriptive study (case series) conducted in private clinic from Jan. 2007 to Sept. 2012.Patients and methods: Under local anasthesia Fine Needle Aspiration Cytology (FNAC) was performed on 250 patients whose sperm count was zero in at least three consecutive semen samples.The size of the testes was measured and recorded for each case. The cytological features of the aspirated cells were described and the patients who have mature spermatozoa were identified for assisted fertilization.Results: Normal spermatogenesis was found in 54 patients (21.6%), complete maturation arrest at primary spermatocytes in 95 patients (38%), complete maturation arrest at secondary spermatocytes in 32 patients (12.8%), germ cell aplasia in 62 patients (24.8%), and atrophic testis in 7 patients (2.8%). The mean age of the patients was 29.8 years. Regarding the greatest dimension of the testes, there was a significant difference in the greatest dimension of atrophic testes and in testes with germ cell aplasia, from the other groups. The procedure was well tolerated by the patients and no complications were encountered. Conclusions: The technique can differentiate obstructive from intrinsic causes of azoospermia. It is simple, quick, cost effective and free from complications.Keywords: FNAC, testis, infertility azoospermia.

الخلاصةالخلفية والأهداف: التطور الحاصل في الإخصاب في المختبر وزرق النطف داخل السايتوبلام بالإضافة إلى التطور في الحصول على النطف من الخصية وفر فرصة لمرضى إنعدام النطف لكي يصبحوا آباء. والهدف من هذه الدراسة هو وصف الخلايا الناتجة عن الرشف بالإبرة الدقيقة وإختيار من لديهم نطف ناضجة للإخصاب المساعد. التصميم والمكان: دراسة وصفية أجريت قي مختبر الباحث الخاص من كانون الثاني 2007 لغاية أيلول 2012.المرضى والطرق: تحت التخدير الموضعي تمت عملية الرشف بواسطة الإبرة الدقيقة لــ 250 مريض يعانون من عدم وجود نطف في السائل المنوي لثلاث فحوصات على الأقل بواسطة السرنج والإبرة الدقيقة23 G ، ثم سحب الخلايا من الخصية وعمل مسحة، ثم ثتبيت المسحة في كحول أثيلي تركيز 95%، ثم صبغها بصبغة هيماتوكسلين أيوسين. وتم وصف الخلايا المسحوبة وتم تشخيص المرضى الذين لديهم نطف ناضجة لغرض إجراء تخصيب مساعد. النتائج: كانت عملية تكوين النطف كاملة في 54 مريض 21,6%، وتوقف كامل لإنتاج النطف في مرحلة الخلايا النطفية الأولية في 95 مريضا 38%. التوقف الكامل لإنتاج النطف في مرحلة الخلايا النطفية الثانوية في 32 مريضا 12,8 %. عدم وجود خلايا بزرات النطاف في 62 مريضا 24,8 %، خصية ضامرة في 7 مرضى 2,8%. وكان المعدل العمري للمرضى 29,8 سنة. بالنسبة للأبعاد القصوى للخصية في حالة ضمور الخصبة وإنعدام الخلايا النطفية كانت تختلف إحصائيا" من المجاميع الأخرى. كانت عملية السحب متحملة من قبل المرضى ولا يوجد مضاعفات. الاستنتاج: بواسطة هذه الطريقة يمكن معرفة أسباب إنعدام النطف (الإنسداد أو أسباب في الخصية). كانت الطريقة سهلة وسريعة ولم تكلف كثيرا". وكانت خالية من المضاعفات.


Article
Embryo Grading and Pregnancy Outcome inWomen of Patients with Azoospermia Following ICSI

Authors: Ula M. AL-Kawaz --- Ban J. Qasim --- Sally A. Shukry --- Ammar M. Qassim
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2016 Volume: 6 Issue: 1 Pages: 61-71
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Azoospermia is defined as the lack of sperm in male ejaculate and is suggested to be present in about 1 % of all males and to account for 10-15% of the causes of infertility in men. The development of intracytoplasmic sperm injection (ICSI) as an effective therapy. Embryo grading has gained special attention in published literature regarding its predictive role in pregnancy outcome following ICSI; however, substantial controversy is present that justified the carrying out of the present study.Objective:To study the association between embryo quality and pregnancy rate in women of males with azoospermia.Patients,Materials and Methods:This prospective cohort study included 60 patients with azoospermia, 15 male with obstructive type and 45 males with non-obstructive type.Those patients were randomly selected form the population of infertile couples who regularly visits the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies and from Kamal Al-Samarrai Hospital -Center for Infertility Treatment and IVF.ResultsPregnancy was encountered in 7 patients (23.33%). It was not statistically associated with type of azoospermia (P=0.388), also it was not affected by age and serum hormonal level, but highly determined by good quality of embryo. Quality of embryo was graded from 1 to 4. The lower the grade, the better the quality of the embryo was. Quality of embryo was not significantly correlated with type of azoospermia, age and hormonal levels.Iraqi Journal of Embryo and Infertility Researches Vol.(6) Special Issue (2016)61ConclusionsGood quality of embryo is a significant predictor of positive pregnancy outcome in ICSI and the quality of embryo is not affected by age of male patient or type of azoospermia.

Keywords

Azoospermia --- ICSI --- embryo grading


Article
Effect of Inhibin-B hormone and its relationship with a number of sex hormones in men with Azoospermia
تأثير هرمون Inhibin-B وعلاقته بعدد من الهرمونات الجنسية عند الرجال المصابين بانعدام النطفية في السائل المنوي

Loading...
Loading...
Abstract

Inhibin-B is produced by sertoli cells and acts as a negative feedback mechanism of follicular stimulation hormone (FSH). In order to evaluate the effect of Inhibin-B in men with Azoospermia, the study measured the concentrations of the following hormones: Inhibin-B, FSH, Luteinizing Hormone Prolactin (PRL), (LH) and Testosterone (Testo). Serum for 30 normal men semen and 50 men with Azoespermia (zero sperm count in sperm). The mean concentration of the hormone Inhibin-B in patients with Azoospermia was (135.7 ±87.6) pg/ml, while in normal men (175.6 ±47.9) Pg / ml with a significant value of the group of healthy patients at a significant level (p≤0.01). The mean serum FSH concentration in patients was (13.7 ± 12.27) lU / L whereas in normal men (4.1 ± 2.84) lU / L, the relationship was significant between patients and healthy at a significant level (P≤0.01) the correlation with the Inhibin-B hormone is reversible relationship. The mean concentration of LH in patients was (12.8±11.87) IU/L and in the healthy (7.9±5.57) IU/L and the relationship between them was significant (P≤0.05) and the correlation with the Inhibin-B hormone is positive relationship. The mean concentration of the Testosterone In the patients was (2.37 ± 1.22) IU / L and in the healthy (5.1 ± 1.96) IU/ L, the relationship was of significant value at P≤0.01 and the correlation with the Inhibin-B hormone is positive relationship, and there is a difference in the mean concentration of PRL between the patients and the healthy where it reaches (8.88 ±5.16) IU / L in patients and (8.03 ± 4.2) IU / L in the healthy. where the relationship was non-significant value between both patients and healthy and the correlation with the Inhibin-B hormone is positive relationship .

ينتج هرمون الانهبين-ب من قبل خلايا سيرتوليsertoli cells ، ويعمل كسيطرة سلبية راجعة على إفراز الهرمون المحفز للجريبات(FSH) . ومن أجل تقييم تأثير هرمون الانهبين-ب في الذكور عديمي النطاف في السائل المنوي Azoospermia، قمنا بقياس تراكيز الهرمونات التالية: الانهبين- ب، FSH، الهرمون اللوتيني,( LH) هرمون الحليب (PRL) وهرمون الشحمون الخصوي (Testo.) في المصل ل 30 رجلاً طبيعي السائل المنوي و 50 رجلاً مصاباً بانعدام النطف في السائل المنويAzoospermia (عدد الحيوانات المنوية صفر في السائل المنوي). فكان متوسط تركيز هرمون الانهبين-ب عند المرضى باللانطفية (135.7±87.6)بيكوغراممل بينما في الرجال الطبيعيين (175.6±47.9) بيكوغراممل مع قيمة معنوية لعلاقة هرمون المرضى بالاصحاء عند مستوى معنوي (p ≤0.01). كان متوسط تركيز هرمونFSH في المصل لدى المرضى هو 13.7)±12.27) وحدة دوليةلتر بينما في الرجال الطبيعيين (4.1±2.84) وحدة دوليةلتر والعلاقة معنوية بين المرضى والاصحاء عند مستوى معنوي (P≤0.01) وعلاقة الارتباط مع الانهبين-ب عكسية. كان متوسط تركيز هرمون ال LH لدى المرضى هو (12.8 ± (11.87 وحدة دوليةلتر ولدى الاصحاء 7.9)±5.57) وحدة دولية / لتر وكانت العلاقة بينهما معنوية عند مستوى معنوي (P≤0.05) وعلاقته مع الانهبين –ب علاقة طردية. وكان متوسط تركيز هرمون الTesto. لدى المرضى 2.37)±1.22) وحدة دوليةلتر ولدى الاصحاء (5.1±(1.96 وحدة دوليةلتر فكانت العلاقة ذات قيمة معنوية عند مستوى معنوي ((P≤0.01 وعلاقة الارتباط مع الانهبين –ب علاقة طردية. بينما كان تركيز هرمون ال PRL. لدى المرضى كان 8.88)± (5.16 وحدة دولية / لتر ولدى الاصحاء كان (8.03 ±(4.2 وحدة دولية/ لتر حيث لم تكن العلاقة ذات قيمة معنوية بين كل من المرضى والاصحاء وكانت علاقته مع الانهبين-ب علاقة طردية.

Keywords

Azoospermia --- inhibin-B


Article
Study of Some Cytogenetic Parameters in Azoospermia and Severe-Oligospermia Patient

Authors: Hussien Jassim Obade --- Hazim I. AL-Ahmed --- Saad Mohamed Nada
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2016 Volume: 24 Issue: 2 Pages: 350-356
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

The aim of this study is to determined micronuclei index, sister chromatid exchange %, in infertile men (azospermai and severe-oligospermia) and compared with fertile men. Thirty patients (20 with azoospermia and 10 with severe oligospermia) and 15 control were studied in this project, The results show significant increase in micronuclei index, sister chromatid exchange %, in patients group compared with control.

ان الهدف من البحث لتحديد فحص دليل تكوين النويات الدقيقة والنسبة المؤية لتبادل الكروماتينات الشقيقة لبيان الدمج في الماده الوراثية. تم دراسة 30 مريض (20 يعانون من انعدام الحيامن و10 مرضى من قلة الحيامن الشديدة) و15 رجال طبيعيين. اظهرت نتائج الدراسة وجود زيادة معنوية في النسبة المؤية للدليل الانقسام الخلوي ، تكوين النويات الدقيقة ونسبة التبادل الكروماتينات الشقيقة للمرضى الذين يعانون من انعدام الحيامن وقلة الحيامن الشديدة مقارنة مع الرجال الطبيعيين.


Article
Embryonic development following the insemination with epididymal sperms of vasectomized mice activated invitro using Glycyrrhiza glabra (Licorice) extract: model for obstructive azospermia

Authors: Saad Al-Dujaily --- Sawsan S. Hamza
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 1 Pages: 43-48
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

BackgroundMany studies found that the Glycyrrhiza glabra (G. glabra) showed a positive effect on the activation of sperm in vitro specifically improve the activity of progressive movement. However, it was not known the impact of this stimulant on the certain sperms parameters in vasal obstructive mammals.ObjectivesThis experiment was designed to examine the possibility of fertilization ova by epididymal sperm of vasectomized mice activated by G. glabra and study the early embryonic development, as a model for obstructive azoospermiaMethodsGlycyrrhiza glabra aqueous extract (0.3 mg/ml culture medium) was used for in vitro direct sperm activation technique. The superovulated female mice (SUO) were divided into two groups, the first group; the insemination of flushed ova was performed with epididymal sperm of vasectomized male mice activated by adding30% of G. glabra to the culture medium (treated group). While the second group, the ova were inseminated by epididymal sperm of vasectomized male mice activated by G. glabra - free medium (control).ResultsThis study showed a highly significant (P <0.01) improvement in certain sperm function parameters i.e the sperm forward movement , morphologically normal sperms (MAS) . There was a significant (P<0.05) increase in fertilization rate (FR) of SUO mice oocyte using 30 % G. glabra medium(75.84%) compared to G. glabra­ Free medium (Hams -F12 medium ) alone (54.43%).By adding 30% G.glabra to the medium , the number of 3-cell and 4-cell embryonic stage of SUO mice was significantly (P <0.05) higher than that of grouping G. glabra- Free medium mice embryo( 62.10 % and 65.78% vs.58.88 and 66.29% , respectively ).ConclusionThis study showed a highly significant (P <0.01) improvement in certain sperm function parameters i.e the sperm forward movement , morphologically normal sperms (MAS) . There was a significant (P<0.05) increase in fertilization rate (FR) of SUO mice oocyte using 30 % G. glabra medium(75 .84%) compared to G. glabra­ Free medium (Hams -F12 medium ) alone (54.43%).By adding 30% G. glabra to the medium , the number of 3-cell and 4-cell embryonic stage of SUO mice was significantly (P <0.05) higher than that of grouping G. glabra- Free medium mice embryo( 62.10 % and 65.78% vs.58.88 and 66.29% , respectively).


Article
TESTICULAR BIOPSY IN AZOOSPERMIC MEN : A STUDY OF THE MORPHOLOGICAL PATERNS IN DUHOK CITY AND AN ATTEMPT TOWARD THE DEVELOPMENT OF A NEW EVALUATION SYSTEM
خزعة الخصية في الرجال المصابين بانعدام الحيامن: دراسة للانماط الشكلية في مدينة دهوك ومحاولة لوضع نطام جديد للتقيم

Author: ALAA H. RAZAK علاء هاني رزاق
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2012 Volume: 6 Issue: 2 Pages: 53-60
Publisher: University of Dohuk جامعة دهوك

Loading...
Loading...
Abstract

Background and objectives Infertility is a common medical problem and about (10-15%) of infertile men had azoospermia. Testicular biopsy in these men has not only a diagnostic role but also a therapeutic one. The frequencies of histopathological patterns are greatly varied in different studies. This is probably attributed to the selection criteria of patients or due to the absence of a uniform system for evaluation of these biopsies. The present work is a cross sectional study which included bilateral testicular biopsy obtained from (80) azoospermic patients over a period of three years from 2009-2011. Biopsies were obtained from Histopathology Laboratory of Azady Teaching Hospital and some private laboratories in Duhok City. The objectives of this study are to determine the histopathological patterns seen in the testicular biopsies in azoospermic male and to develop a uniform system for evaluationof these biopsies.Methods This study included (80) infertile men with azoospermia. Bilateral testicular biopsies were examined and the histopathological findings were classified into three major categories; in the first one, sperms are seen regardless their number, in the second category, there is maturation arrest and in the last category, there is absence of germinal cells regardless the presence of Sertoli cells.Results This study revealed that (37.5%) of the included patients had sperms in their testicular biopsies, (33.7%) had maturation arrest and (28.7%) had no germinal cells.Conclusions There are great dissimilarities in the patterns of testicular biopsy among different workers. In the present work, the frequencies of normal spermatogenesis (regardless the number of sperms) (37.5%), maturation arrest (33.7%) and absence of germ cells (28.7%) are nearly equal. While none of our patients had generalized fibrosis. The criteria for selection of azoospermic patients who will be subjected to testicular biopsy need to be standardized. The proposed evaluation scheme is informative and reproducible amongpathologists and easily understandable by the surgeon.

15 %) من الرجال لاتوجد لدیهم حیوانات منویه في السائل - خلفیة واهداف البحث: العقم مشكله طبیه شائعه و ( 10 المنوي. فحص خزعة الخصیه له دور تشخیصي و علاجي. تختلف نسب المشاهدات النسیجیه لعینة الخصیه كثی ا ر في د ا رسات مختلفه. و قد یعود سبب الاختلاف الى طریقة اختیار المرضى المرشحین لاج ا رء هذه العملیه او الى عدم وجود نظم متفق علیه لتقییم خزعة الخصیه. ان هذا البحث هو د ا رسه لخزع الخصیتین ماخوذه من ثمانین مریضا مصابین 2011 في مختبر الفحص النسیجي لمستشفى - بانعدام الحیامن في السائل المنوي جمعت خلال ثلاث سنوات من 2009ا ا زدي التعلیمي و بعض المختب ا رت الاهلیه في مدینة دهوك. وتهدف هذه الد ا رسه لمعرفة الانماط الشكلیه لنسیج الخصیه في هؤلاء المرضى و لوضع نظام جدید لتقییم خزع الخصیه.طرق البحث: شملت هذه الد ا رسه ( 80 ) مریضا لدیهم انعدام الحیوانات المنویه في السائل المنوي و اخذت منهم خزع من كلا الخصیتین و تم تصنیف الانماط النسیجیه الى ثلاثة انواع. النوع الاول لدیهم حیامن في الخصیه بغض النظر عن عددها و النوع الثاني لدیهم توقف نضج الخلایا المنتجه للحیامن و النوع الثالث لاتوجد لدیهم الخلایا الام المولده للحیامن.النتائج: اظهرت الد ا رسه ان ( 37.5 %) من المرضى المشمولین بالد ا رسه لدیهم حیامن في الخصیه و ( 33.7 %) لدیهم توقف النضج و ( 28.7 %) لیس لدیهم خلایا ام مولده للحیامن.الاستنتاجات: هناك فروقات كبیره في نتائج الد ا رسات المختلفه حول هذا الموضوع. اظهرت هذه الد ا رسه ان نسب الانماط الثلاثه وهي وجود الحیامن في الخصیه ( 37.5 %) و توقف النضج ( 33.7 %) و غیاب الخلایا الام المولده للحیامن %28.7 ) هي نسب متقاربه. و لم یشخص اي مریض بالتلییف العام للخصیه. و لذلك یجب وضع اسس لاختیار ) المرضى لهذه العملیه. و ان استعمال نظام التقییم هذا سهل و یتفق علیه اختصاصیي التشخیص النسیجي و سهل الفهم بالنسبه للج ا رحین.


Article
In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients

Author: Saeeda A. M. Alanssari
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 4 Pages: 394-399
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients. Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes. Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied. Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte percentage was significantly higher (P


Article
Testicular biopsy in the study of azoospermia

Author: Ala'a Al-Deen Al-Dabbagh
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2012 Volume: 11 Issue: 2 Pages: 7-10
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Aims: to evaluate the role of testicular biopsy in the study ofazoospermic patients.Patients and methods: a total of 67 infertile men with azoospermia werestudied over 5 years period (February 2005 – March 2009). Their meanage was 33 years. They underwent bilateral testicular biopsy undergeneral anaesthesia and the specimens were categorized according tohistopathological patterns.Results: the histological evaluation of the testes revealed normalspermatogenesis in 12 patients (17.9 %), hypospermatogenesis in 4 (5.9%), spermatogenic arrest in 39 (58.2 %), sertoli cell only in 7 (10.4 %) andcomplete tubular hyalinization in 5 patients (7.4 %).Conclusions: This study highlights the role of testicular biopsy as areliable and useful technique for the investigation of patients withazoospermia.


Article
Cytological findings of testicular fine needle aspiration in a

Author: Basim Sh. Ahmed
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2012 Volume: 11 Issue: 2 Pages: 24-28
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Azoospermia account for about 10–15% of male infertilitycases. It denotes the final result of different testicular alterations,ranging from non-obstructive to obstructive azoospermia which couldbe corrected surgically. The former is associated with impairment ofspermatogenic process, including hypospermatogenesis, maturationarrest and complete absence of germ cells-Sertoli cell-only syndrome(SCOS). To ascertain such azoospermia aetiology, Testicular biopsy isconsidered as the standard method. However, the popularity of Fineneedle aspiration (FNA) cytology has gained as simple and minimallyinvasive way that can help in assessing testicular function accurately.Aims: To determine causes of azoospermia, by assessing thecytological findings in testicular FNA.Patients and Methods: A prospective study for 95 azoospermicpatients was conducted from Jan 2010-Dec 2011 at private laboratoryusing FNA procedure. Here, detailed history and physical examinationwere done along with semen analysis to confirm true azoospermia.Both testes were aspirated to obviate sampling errors whenappropriate. Routine Haematoxylin and Eosin staining was alsoperformed on the smears.Results: Adequate samples were obtained from 84 (88.4%) cases,while 11 patients (11.6%) had scanty smears where cytological diagnosiscould not be obtained. These adequate smears were categorized asnormal spermatogenesis in 38 (45.2%) patients, maturation arrest atspermatocyte/spermatid level, and Sertoli cell only syndrome in 27(32.2%) and 19 (22.6%) patients, respectively.Conclusions: FNAC could permit characterization of specificcytological pictures related to testicular damage nature. It is able toidentify different azoospermic subjects and represents a reliable andprognostic parameter of seminiferous epithelium status.

Listing 1 - 10 of 28 << page
of 3
>>
Sort by
Narrow your search

Resource type

article (28)


Language

English (21)

Arabic and English (4)

Arabic (1)


Year
From To Submit

2019 (1)

2018 (2)

2017 (2)

2016 (5)

2015 (3)

More...