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Amplification and cloning the gene of bovine leukocyte α-A-interferon in cells of Escherichia Coli
تضخيم وكلونة جين الانترفيرون الفا من كريات الدم البيضاء للأبقار في بكتريا القولون Escherichia Coli

Author: Arafat A. Muttar عرفات عبد الرزاق مطر
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 3B Pages: 1255-1258
Publisher: Baghdad University جامعة بغداد

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Abstract

Interferon’s plays a role in innate immune responses through upregulation of costimulatory molecules and induction of proinflammatory cytokines. Interferon alpha (IFN α) type of Interferons. The present study characterized IFNα cDNA . The interferon’s play a great role in protection from infections, caused by microorganisms, and have powerful antiproliferative and immunomodulation activity. In this study DNA was isolated from bovine blood leukocyte, which was used in the quality of matrix for amplification of α-interferon gene with the use of PCR, and isolation of gene α-interferon and transformation in vector pUC18 and expression vector pET24b (+). All plasmids contained an additional DNA fragment size corresponding to the gene of bovine α-A-interferon.

الانترفيرونات تلعب دورا في الاستجابة المناعية الذاتية من خلال اعادة التنظيم وتحفيز السايتوكاينيز ، ولها نشاط مناعي. الانترفيرون ألفا (IFN α) احد أنواع الانترفيرونات. في هذه الدراسة توصيف الانترفيرن الفا. الانترفيرون تلعب دور كبير في الحماية من الاصابات المتسببة بواسطة الاحياء المجهرية ولها نشاط قوي ضد التكاثر والتنظيم المناعي. في هذه الدراسة تم عزل الحمض النووي من كريات الدم البيضاء من الابقار، وتضخيم جين الانترفيرون المعزول من الدنا بمساعدة PCR، ومن ثم التحول في ناقل pUC18 وبعدها التحول البكتيري بواسطة ناقل (+)pET24b. حيث أظهرت النتائج وجود جين الانترفيرون الفا البقري في جميع البلازميدات بعد الكلونة.


Article
Testing The Activities of Some of The Inside and Outside Cellular Compounds Produced by The Algae Chlorococcum infusionum and Scenedesmus obliquus Against Some Bacteria

Authors: Suaad H. Ali Al-Badri --- Abdul-Wahab Resan AL-Ebady
Journal: Journal of Education for Pure Science مجلة التربية للعلوم الصرفة ISSN: 20736592 Year: 2019 Volume: 9 Issue: 2 Pages: 133-139
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Abstract:In this study Chlorococcum infusionum and Scenedesmus obliquus were isolated ،purified andidentification From the Euphrates River at the city of Nasiriyah. The water of this canals originally fromTigris River. Ch-10 culture media was used for their cultivation in suitable laboratory conditions(25c°,200μE/m2/sec) for 16:8 hrs. Light: dark. Each culture was harvested at the end of exponential phase.Organic solvents used for extraction were Ethanol، Hexane and Methanol 95% to extract the crude activeIntracellular and Extracellular substances, and evaporated down to dryness .Antibacterial activity of thesedifferent extracts were evaluated against 6 strains of gram positive bacteria and gram negative bacteria,Agar diffusion method was used in this evaluation.

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