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Article
Monitoring of patients undergoing examination of bone marrow aspiration and biopsy
مراقبة المرضى الذين تحملوا فحص سحب واخذ خزعة من نخاع العظم

Author: Hakemia Shaker Hasian حكيمه شاكر
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2007 Volume: 20 Issue: 4 Pages: 102-106
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract:Background: Monitoring of the patient is to improve the outcome of the bone marrow aspiration and biopsy procedure by decreasing the risk of the procedure itself Objective: 1-To monitor the heart rate , and blood pressure of the patient before ,during ,and until 6 minutes after the needle of bone marrow biopsy and aspiration has been with drawn,2- Early detection of any signs and symptoms of distress 3-to monitor the ECG abnormalities that might occur during the bone marrow aspiration and biopsyMethods: The study consisted of 33 patients referred consecutively to the Baghdad teaching hospital 7th floor ( bone marrow aspiration and biopsy clinic) Before bone marrow aspiration and biopsy , baseline 12 leads ECG ,blood pressure, and heart rate were recorded .Lead II ECG, heart rate ,blood pressure were continuously recorded during the procedure until 6 minutes after the needle had been with drawn and 12 lead ECG ,blood pressure and heart rate gain recorded at the time .Results: Heart rate , systolic, diastolic blood pressure before the procedure were significantly increase in comparison with during period of the bone marrow aspiration and biopsy and decreases in number of patients suffering from nausea, vomiting ,pallor, chest pain, dyspnea, general pain when comparing to non suffering from signs and symptomsConclusion: Electrocardiographic changes are rarely found in patients undergoing bone marrow aspiration. According to the results the investigatore recommended to preparing and training of bone marrow aspiration and biopsy clinic staff for patient care before, during and after bone marrow aspiration and biopsy

الملخص:خلفية الدراسة:-مراقبة المريض بحسين من أجراء سحب واحذ خزعة من نخاع العظم وذلك بتقليل نتائج خطورة الأجراءالهدف: 1- مراقبة ضربات القلب وضغط الدم للمرض قبل وخلال و لفترة زمنية لمدة 6 دقائق بعد سحب الإبرة. 2- الكشف المبكر عن أي علامة وعرض من علامات الإجهاد. 3- مراقبة الحالات الشاذة في تخطيط القلب الكهربائي خلال اجراء سحب واخذ خزعة من نخاع العظم.الطريقة :شملت الدراسة على (33)مريضا أحيلوا بصورة متعاقبة إلى مستشفى بغداد التعليمي / الطابق السابع (عيادة مرضى سحب واخذ خزعة من نخاع العظم ) . قبل أجراء سحب واخذ خزعة من نخاع العظم الخطوات الرئيسية تم تسجيل 12 قطب من تخطيط آلقلب الكهربائي وضغط الدم وسرعة ضربات القلب وباستمرار تم تسجيل سرعة ضربات القلب من القطب الثاني لتخطيط القلب الكهربائي مع ضغط الدم خلال أجراء سحب واخذ خزعة من نخاع العظم ولمدة 6 دقائق بعد أجراء سحب واخذ خزعة من نخاع العظم.حيث سجل مرة ثانية 12 قطب من تخطيط القلب الكهربائي ,وضغط الدم وسرعة ضربات القلب في ذلك الوقت.النتائج :هنالك زيادة ذات دلالة معنوية في سرعة نبضات القلب. و ضغط الدم العالي مقارنة بفترة قبل وخلال أجراء سحب واخذ خزعة من نخاع العظم وقلة في عدد المرضى الذين عانوا من غثيان وتقيء وشحوب لون الجلد والآلام في الصدر مع ضيق في التنفس وألم عام وشامل مقارنة بأولئك الذين لم يعانوا من العلامات والأعراض المرضية . النصائح: التغير في تخطيط القلب نادر حدوثه مع المرضى الذين يخضعون لأجراء سحب واخذ خزعة من نخاع العظم . وطبقا لهذه النتائج توصى الباحثة بتهيئة وتدريب الملاكات العاملة بوحدة عيادة مرضى سحب واخذ خزعة من نخاع العظم حول العناية التمريضية قبل ,خلال وبعد سحب واخذ خزعة من نخاع العظم


Article
Mus musculus في الفئران البيض (Coker تأثير إطعام بذور القطن صنف ( 310

Author: علي عبيس عبد الشمري
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2010 Volume: 13 Issue: 3 Pages: 50-57
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The effect of feeding with cottonseed(Coker 310) which is cultivated in Iraq onsome parameters on white mice Mus musculuswas studied. The parameters were grosscharacters such as body weight, and someanatomical parameters; weight of liver, kidneyand spleen. In addition the effect on male germcells was studied by measuring the sperm headabnormalities, some cytogenetic parameterswere studied as well, among these, theinduction of micronuclei (Mn) and mitoticindex (MI) in femur bone marrow.Results showed that there was nonsignificantdifferences in body weight betweencontrol group and treated group after ten daysof feeding with cottonseeds. Similar resultswere obtained for weights of liver and kidney.The average weight was 1.47g for controlgroup and 1.25g for treated group, kidneyweights were 0.44g (control) and 0.395g forcottonseed group. On the other hand, spleenweights were affected significantly (P≤0.05) asthe weight of control groups was 0.104g andthe treated group was 0.066, ie., there was36.5% reduction.The effect on male germ cells wasstatistically significant (P≤0.05), since thetreatment (ie, feeding with cottonseed)increased the level of abnormal sperms to5.5/1000 sperm compared to control group(1.65/1000 sperm).Cytogenetic parameters were significantlyaffected (P≤0.05) as the Mn raised to 6.07/1000 cells compared to background level3.2/1000 cells (control group), while the MIreduced in treated group to 3.22 compared to7.18 of the control group

الذي يزرع في العراق في بعض الصفات للفئران البيض (Coker تم دراسة تأثير إطعام بذور القطن صنف ( 310درست الصفات المظهرية الواضحة مثل وزن الجسم، وبعض الصفات التشريحية بقياس أوزان بعض Mus musculusالأعضاء الداخلية منها وزن الكبد ووزن الكلى ووزن الطحال، فضلاً عن تأثيرها في الخلايا الجنسية الذكرية بقياس عددالنطف المشوهة. ودرست بعض مؤشرات الوراثة الخلوية بقياس تكون النوى الصغيرة وقياس معامل الانقسام في خلايا نخاععظام الفخذ.أوضحت النتائج عدم وجود فروق معنوية في وزن الجسم لذكور الفئران البالغة بعد الإطعام لمدة عشرة أيام، ولم تكنإذ كان معدل أوزان الكبد لمجموعة السيطرة 1.47 غم ولمجموعة المعاملة ،(P≤ الفروق معنوية في أوزان الكبد والكلى ( 0.051.25 غم، أما الكلى فكان المعدل لمجموعة السيطرة 0.44 غم ولمجموعة بذور القطن 0.395 غم. ولكن وزن الطحال تأثروبشكل كبير وبفروق معنوية إذ كان معدل الوزن لمجموعة السيطرة 0.104 غم وبالنسبة لمجموعة بذور القطن 0.066 غم أي.(P≤0.05) % بنسبة انخفاض وصلت إلى 36.5أما التأثير في الخلايا الجنسية فقد ارتفع عدد النطف المشوهة ليصل إلى ثلاثة أضعاف الحالة الطبيعية التي كانت بمعدلوأشارت .(P≤ 1000 نطفة عند إطعام بذور القطن وبفروق معنوية ( 0.05 / 1000 نطفة في حين وصلت إلى 5.5 /1.651000 خلية / دراسة مؤشرات الوراثة الخلوية إلى ارتفاع عدد النوى الصغيرة وبشكل معنوي إذ كانت في الحالة الطبيعية 3.21000 خلية في حالة استعمال بذور القطن، وانخفض معامل الانقسام وبفرق معنوي إلى 3.22 مقارنة / ووصلت إلى 6.07. بالحالة الطبيعية الذي كان 7.18

Keywords

Cottonseed --- mice --- feeding --- bone marrow


Article
Complications of Bone Marrow Aspiration and Biopsy: a New Unexpected Ris

Author: Najlaa Badr Alawadi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: supplement Pages: 712-715
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Bone marrow examination represents an essential tool for diagnosis and monitoring of haematological and non-haematological disorders. Although some hazards are recognized, bone marrow aspiration and trephine biopsy are generally thought to be safe procedures. OBJECTIVE:The aim of this study is to identify the possible complications associated with the bone marrow aspiration and biopsy procedures.METHODS: In this study, 2156 cases of bone marrow aspiration with or without trephine biopsy were done in Merjan Teaching Hospital, Babil governorate, Iraq during a five year period from 1st Feb 2006 to 1st Feb 2011. These procedures were done under local anesthesia. The complications associated with these procedures were reported.RESULTS: A new previously unreported complication was seen in 2 patients as they developed sudden apnea and loss of consciousness.The final diagnosis in both patients was acute lymphoblastic leukemia with huge mediastinal widening and they were lying in prone position during the procedure.Bleeding was seen in 2/2156 (0.09%) cases. Prolonged pain was documented in one case. No infection was reported.CONCLUSION: Bone marrow examination is generally safe, however apnea and loss of consciousness can occur in patients with a huge mediastinal mass compressing trachea and/or major chest vessels when the procedure is done in prone position.KEY WORDS: Bone marrow aspiration.


Article
A Clinical-Hematological Study of Pancytopenia Patients Attending Nanakaly Hospital in Erbil City

Authors: Alan Isaac Isho --- Nawsherwan Sadiq Mohammad
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2016 Volume: 5 Issue: 1 Pages: 129-142
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Background: Pancytopenia is a triad of low hemoglobin, white blood cells and platelets.Although it is a common clinical problem with an extensive differential diagnosis, there is arelatively little discussion of this abnormality in major textbooks of internal medicine andhematology.Objectives: This study aimed to determines the etiology and clinical profile of pancytopenicpatients attending Nanakaly hospital.Materials and Methods: During a period of 6 months, 60 pancytopenic patients attendedNanakaly hospital, their ages ranged between one-81years. A control group of 50 age-matchedapparently healthy person were tested for complete blood picture and reticulocyte count. History,physical examination and hematological parameters at presentation were recorded. Hematologicalprofile included hemoglobin, total and differential leukocyte count, platelet count, reticulocytecount, peripheral blood and marrow smears together with marrow biopsy were assessed.Pancytopenic cancer patients on chemotherapy were excluded. Pancytopenia was defined ashemoglobin less than 10g/dl, WBC less than 4 x109/L and platelet count less than 150 x 109/L.Results: The mean Hb concentration, WBC count and platelet count in studied group weresignificantly lower than in control group. Hematological malignancies were the commonest causeof pancytopenia and accounted for (51.7%), they included: Acute leukemia (35%),myelodysplastic syndrome (11.7%), hairy cell leukemia (3.3%) and myelofibrosis (1.7%). Aplastic and megaloblastic anemia each of them accounted for (16.7%), hypersplenism wasresponsible for (10%). Other less common causes included enteric fever, kalaazar and secondarymetastasis each of them accounted for (1.7%). Pallor was present in every case. Fever was presentin (63.3%) and (25%) had bleeding manifestations at the time of presentation Conclusions: The most common causes of pancytopenia were acute Leukemia, aplastic anemiaand Megaloblastic anaemia, but rare causes like myelofibrosis, enteric fever, kala azar andsecondary metastasis should also be kept in mind.


Article
Utility of simultaneous assessment of bone marrow aspirates and trephine biopsy sections in various haematological disorders

Authors: Vandana Puri --- Pooja Sharma --- Mrinalini Kotru --- Meera Sikka --- et al.
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2018 Volume: 7 Issue: 1 Pages: 26-32
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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BACKGROUND: Bone marrow examination is crucial diagnostic modality for evaluation of varioushematological and nonhematological disorders. However, marrow aspirate smears and biopsysections, even though performed simultaneously, are often assessed at different points of time dueto different processing methods. This sometimes results in discordance in diagnosis which adds tothe diagnostic dilemma and delays the treatment.AIM: This study aims to compare the diagnostic accuracy and the rate of concordance between thetwo modalities of bone marrow examination.MATERIALS AND METHODS: Three hundred simultaneously performed bone marrow aspiratesand BM trephine biopsies were retrospectively analyzed over a period of 1 year. The presence orabsence of concordance was recorded. The reasons for inconclusive reports were also recorded.The concordance rates for different hematological disorders were calculated and recorded as highfor >80%, moderate for 50%–80%, and low for <50%. The findings of discordant cases and reasonsfor discordance were also tabulated.RESULTS: A high concordance was found in cases of megaloblastic anemia, leukemias,non‑Hodgkin’s lymphoma, and multiple myeloma; moderate concordance was found in hypoplasticmarrow and concordance was low in Hodgkin’s lymphoma, chronic myeloid leukemia (CML) in blastphase, metastatic, and granulomatous involvement of bone marrow.CONCLUSION: Bone marrow aspiration alone is sufficient for the diagnosis of megaloblastic anemiaand most of the hematological malignancies. Bone marrow biopsy is more appropriate for detectionof disorders with focal marrow involvement such as lymphoproliferative disorders, metastatic cancer,focal blast crisis in CML, granulomatous lesions, and hypoplastic marrow. However, it is stronglyrecommended that both should be reviewed simultaneously to ensure maximum diagnostic accuracy.

Keywords

Aspirate --- biopsy --- bone marrow --- concordance


Article
BONE MARROW NECROSIS IN SICKLE CELL DISEASE, A CLINICAL & PATHOLOGICAL STUDY

Authors: Zuhair A Al-Barazanchi --- Alwan H Al-Shiwaeli
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2007 Volume: 13 Issue: 1 Pages: 1-88
Publisher: Basrah University جامعة البصرة

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Abstract

Zuhair A Al-Barazanchi*, Alwan H Al-Shiwaeli#*M.Sc Haematol. Consultant Haematologist, Post-graduate Supervisor & Lecturer, Laboratory Department, Basrah General Hospital.#M.Sc Haematol. Specialist Haematologist, Al-Faeha General Hospital.Bone marrow necrosis (BMN) is a rare clinical-pathological entity. It is mostly associated with post-mortem changes. Ante-mortem existence of BMN is quite rare and usually indicates a poor prognosis. However, its association with non-hematological malignancies seems not to be as poor prognostic feature as in post-mortem changes. One of the most commonly disorders associated with BMN is sickle cell disease (SCD), which is common among people in Basrah province. The current study probably can put light on the incidence of such pathology among those with SCD, its most clinical presenting features and its relation with specific sickle disease genotypes. The incidence of BMN in this study was 4.9 %. The mostly encountered features with wide spread necrosis were bone pains, fever, and pallor. Peripheral blood showed a florid leuco-erythroblastic picture with reticulocytosis and leucopenia in another case with focal necrosis. On comparison with those patients without BMN, patients with BMN showed a significantly lower Hb concentration, higher Hb S concentration, lower Hb F concentration, smaller splenic size, higher number of irreversibly-sickled cells and more frequent painful crises during their life. Those results were compatible with some observations and contradict with other. However, there is no previous study conducted in Iraq to compare with.


Article
Bone Marrow Involvement in Malignant Lymphomas (Non-Hodgkin's)
أورام الغدد اللمفاوية نوع غير هودجكن في خزع نقي العظم

Author: Eman Sadiq Jalal د. إيمان صادق جلال
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2008 Volume: 21 Issue: 4 Pages: 326-330
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Bone marrow biopsies are taken routinely in the initial investigation of patients with non-Hodgkin, s lymphomas to estimate the progression of disease at time of presentation (staging) and to type mode of proliferation (growth pattern) in the bone marrow.Method: Initial pretreatment bone marrow biopsies were taken from twenty-four patients diagnosed by the established criteria on lymph node biopsies as suffering from one of the non-Hodgkin, s lymphomas. All biopsies were examined histologically.Result: Frequency of bone marrow involvement in different types of non-Hodgkin, s lymphomas (at time of initial diagnosis) was found in 75%. In the low grade lymphoma the age incidence was 28-75 years (mean50 years); while for the high grade type it was 6-25 years (mean15years).Conclusion: The results clearly demonstrate that at first presentation over half of the patients with malignant lymphoma have positive bone marrow biopsies indicating disseminated disease, i.e. stage IV. Malignant lymphoma of low grade malignancy more frequently involves the bone marrow than malignant lymphoma of high grade malignancy. Thus, in the bone marrow biopsy the clinician has a tool at his disposal which supplies decision information on the diagnosis and therapy of any given patient with non-Hodgkin, s lymphomas. Keywords: Non-Hodgkin's lymphomas, bone marrow biopsy.

تم الفحص النسيجي لأربع وعشرين عينة من خزع نقي العظم لمرضى أورام الغدد اللمفاوية نوع غير هودجكن لتحديد نوع وكمية المرض في نقي العظم ومعرفة مرحلة المرض وقت التقديم . تبين احتواء خزع نقي العظم للمرض عند اكثر من نصف المرضى المصابين باورام الغدد اللمفاوية نوع غير هودجكن, كما ان احتمالية شمول نقي العظم بالاصابة في ورم الغدد اللمفاوية الخبيث المنخفض الدرجة اكثر بكثير من احتمالية اصابة نخاع العظم بورم الغدد اللمفاوية من خبث الدرجة العالي حيث يصل الى 83% في الحالة الاولى .هذه الملاحظات تشير بشكل حاسم بأن فحص خزع نقي العظم اداة تشخيصية مفيدة للتصنيف النسيجي للمرض ومدى انتشار المرض عند اي مريض مصاب بورم الغدد اللمفاوية نوع غير هودجكن والذي سيؤثر بشكل فاعل على نوع العلاج المستخدم مستقبلا.


Article
Flowcytometry for Estimating Efficient Transfection of Bone Marrow Cells with BCR-ABL Gene

Authors: Rihab Nasr --- Manal Adnan Habib --- Ahmad Iskandarani
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: supplement Pages: 649-653
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Chronic myeloid leukaemia can be accurately modeled in laboratory mice by the retroviral transfer of a BCR-ABL gene into murine hematopoietic stem and progenitor cells, followed by transplantation of these cells into irradiated recipient mice.OBJECTIVE:The use of Retroviral vector for transfer of BCR-ABL gene into murine bone marrow cells (BMC) and measurement of efficiency of transfection by flowcytometry.METHODS:Murine bone marrow cells obtained from mice were cultured in a medium containing the supernatant of BCR-ABL (p210) transfected platinum E cells which is rich in retroviral vector carrying the BCR-ABL (p210) gene.The vector express green fluorescent protein (GFP) as well so that the efficiency of transfection of murine BMC with the target gene was able to be measured using flowcytometry.RESULTS:The use of the retrovirus packaging cell line enhanced the transduction of BMC with the retroviral vector and efficiency of transfection was 72% as measured by the flowcytometry.CONCLUSION:Transfer of BCR-ABL gene into murine BMC by retroviral vector that carry GFP marker which allowed the estimation of transfection efficiency by the flowcytomery


Article
Bone Marrow Reticulin Content In Acute Leukemia

Author: Eman Sadiq Jalal, MSc. ايمان صادق جلال
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2006 Volume: 48 Issue: 3 Pages: 319-322
Publisher: Baghdad University جامعة بغداد

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Background: Increase in marrow reticulin has long been recognized in primary myelofibrosis, but little was known about the reticulin structure of leukemic marrows, there has been a tendency to accept an increase in marrow reticulin as evidence of myelofibrosis. Similarly, there has been a tendency to diagnose as "acute myelofibrosis" cases of acute leukemia with brisk reticulin and early collagen production. This study was undertaken to determine incidence, type and pattern of fibrosis in the bone marrow of patients with acute leukemia, both acute lymphoblastic and acute myeloid leukemia.Method: thirty-five bone marrow biopsy specimens from patients with acute leukemia were examined histologically; connective tissue stains were applied for stromal studies.Result: an increase in marrow reticulin is common at presentation in patients with acute leukemia, effective anti-leukemic therapy results in resolution of the increased marrow reticulin.Conclusion: The reticulin type of fibrosis is the common type encountered in acute leukemia which is of diffuse pattern, higher incidence of bone marrow fibrosis in patients with acute lymphoblastic leukemia than those with acute myloid leukemia. Fiber bone formation is found in 10% of cases of acute lymphoblastic leukemia ,but is not extensive and well developed as in cases of primary myelofibrosis.Keywords: acute leukemia, bone marrow biopsy, reticulin content. __________________________________________________________________________________________


Article
Comparison between three different protocols for isolation and culture of mouse bone marrow derived mesenchymal stem cells
دراسة مقارنة لثلاثة طرق لعزل وزراعة الخلايا الجذعية اللحمية المشتقة من نخاع عظم الفأر

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Bone marrow derived mesenchymal stem cells (BM-MSCs) represent a promising source for cell therapy, As they can differentiate into bone, cartilage, fat, tendon and many other organ progenitor cells. Although the culture of MSCs has been studied for over 30 years. The identification of standard protocol for isolation and characterization have yet to be developed. A comparison study was made on three different isolation techniques, which include: direct plating, red blood lysis buffer strategies, and density gradient (DG) method (using two different gradient media: Ficoll and Percoll) to find the optimal isolation and culture condition for adequate amount of MSCs for clinical use.The results demonstrate that direct plating of whole bone marrow (BM) suspension provides a suitable alternative protocol for isolation of BM-MSCs with minimal requirements, which mainly based on the frequent medium change in primary culture. The BM crud cell suspension were isolated by aspiration from albino mice and cultured in fresh complete isolation media (CIM) RPMI-1640/10%FBS. Adherent cells from BM cells suspension were MSCs which then expanded by complete expansion media (CEM) α-MEM/ 10% FBS. By concluding, direct plating of whole bone marrow represent the alternative method for isolating of mesenchymal stem cells from small specimen of bone marrow. By concluding, the present study was designed to investigate the optimal technique for the isolation of BM-MSCs for use in tissue engineering and regenerative medicine.

تعد الخلايا الجذعية اللحمية المشتقة من نخاع العظم مصدرا واعدا في العلاج الخلوي. لقابليتها على التمايز الى خلاياانسجة العظم، الغضروف،الخلايا الدهنية والوتر والكثير من الخلايا المولدة للأعضاء. رغم ان زراعة الخلايا الجذعية اللحمية درست خلال الثلاثين السنة الماضية الا انها لم توفر نمط قياسي لعزل وتمايز هذه الخلايا لحد الان لذلك تم في هذه الدراسة مقارنه ثلاث طرق مختلفة لعزل الخلايا الجذعية المشتقة من نخاع العظم وهي: العزل بواسطة الطريقة المباشرة، استعمال الدارئ المحلل لكريات الدم الحمر، وباستعمال الوسط المتدرج الكثافة وذلك لإيجاد الطريقة والظروف المثلى للعزل والزراعة لحصد كميات ملائمة من الخلايا الجذعية اللحمية. أوضحت النتائج ان الطريقة المباشرة لزراعه معلق نخاع العظم الخام تمثل أفضل طريقه لعزل الخلايا اللحمية، والتي تقوم أساسا على التغيير المتكرر للوسط الزرعي في المزرعة الأولية. تم جمع عالق نخاع العظم الخام من الفئران البيض وانمائها في الوسط الزرعي العازل(RPMI-1640/10%FBS). كانت الخلايا الملتصقة من معلق نخاع العظم في اوعية الزرع هي الخلايا الجذعية اللحمية والتي تم ادامتها بالوسط الزرعي (MEM/ 10% FBS). وعلية صممت الدراسة الحالية لايجاد الطريقة المثلى لعزل الخلايا الجذعية اللحمية من نخاع العظم المستخدمة في هندسة الانسجة والطب التجديدي.

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