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Article
clinical and histological evaluation of the effect of bovine saliva on the experimentally induced open wounds on rabbit
التقييم السريري والنسيجي للتاثير الموضعي للعاب الابقار على الجروح الجلدية المفتوحة والمستحدثة تجريبيا في الارانب

Author: A.A.Abbas Alfaris A.A.sawad عبد الباري عباس الفارس علاء عبد الخالق سواد
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2007 Volume: 6 Issue: 1 Pages: 45-53
Publisher: Basrah University جامعة البصرة

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Abstract

The use of bovine saliva as a wound dressing rriaterial. In the present study, bovine saliva was_applied to experimental by second intention on the eilrperimental wounds in rats , and it's effects on_ epithelization, wound contraction newly formed granulation tissue. T f I. We evaluated the time interval required for wound healing using a standard wound with andwithout bovine saliva. The bovine saliva did interfere with a normal development of wound healing. -Histological evaluation was considered to parameters the effect of bovine saliva on woundhealing. The effect appear to be due to an increase collagen activity , consequently improving the T_ collagen matrix and enhancing the breaking strength by lysosomes with saliva

استخدم في هذه التجربة لعاب الابقار كاحد مواد التضميد بوضعة تجريبيا على الجروح المستحدثة في الجرذان من خلال تاثيره على عملية الترميم الظهاري وتقليص الجرح وتكوين نسيج حبيبي جديدتم تقييم الفترات الزمنية لالتئام الجروح من خلال استحداث جروح نموذجية باستعمال لعاب الابقار الذي يتداخل مع التطور الطبيعي لعملية الالتئاماظهر التقييم النسيجي تغيرات كبيرةلتاثير لعاب الابقار على التئام الجروح نتيجة الزيادة في الفعالية الكولاجينية اضافة الى زيادة في الوسادة الكولاجينيةوزيادة قوة التحطيم الاليسوسومي للعاب

Keywords

Bovine --- Saliva --- wound healing


Article
MOLECULAR AND SEROLOGICAL IDENTIFICATION OF FOOT-AND-MOUTH DISEASE VIRUS SEROTYPES IN CATTLE OF BASRAH PROVINCE
التشخيص الجزيئي والمصلي للانماط المصليه لفايروس مرض الحمى القلاعيه في ابقار محافظة البصره

Author: Adnan M. Al-Rodhan Zainab M. Salem عدنان موسى الروضان زينب مجيد سالم
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2014 Volume: 13 Issue: 1 Pages: 180-197
Publisher: Basrah University جامعة البصرة

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Abstract

This study was conducted on serum from 6 different regions covering all parts of the Basrah province during the period from October 2012 to June 2013 in order to determine the prevalence of foot and mouth disease in bovine species. From the serology results, the overall prevalence of this disease was(72.7%.) . Of the 165 samples subjected to Nonstructural protein (NSP) enzyme linked immunosorbent assay (ELISA) screening test, 120 were interpreted as positive representing (72.7%.) while the other 45 samples were negative representing 27.3%. The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.05). RT-PCR base detection of FMDV for primary and serotype specific diagnosis was used. Different types of clinically positive samples of FMD were collected from the cattle which includes samples from mouth vesicles, serum and saliva .RT-PCR based identification was done with( universal primer sets 1F / 1R, A- 1C562, O-1C272 and As1-1C505) with expected band of( 328, 866, 639 and 911 bps.respectively) . Of eighty three samples collected, only 68(81.9%) were successfully amplified by RT-PCR, and of these, only 19(22.9%) samples were found to be serotype A-1C562 positive and 26(31.3%) were serotype O-1C272 positive and nil for serotype As1-1C505. The frequency of foot-and-mouth disease viral genome presence in cattle had no significant differences based on sex (P>0.05) while the difference among age groups was significant (P < 0.05)

اجريت هذه الدراسه في6 مناطق مختلفه ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012الى شهر حزيران من عام 2013 على عينات من المصل وذلك لتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار.من نتائج الفحص المصلي ان النسبه الكليه لانتشار هذا المرض كانت72.7%.من 165عينه خضعت لاختبار الممتز المناعي المرتبط بالانزيم المعتمد على مستضد البروتين اللا تركيبي تاكدت ايجابية 120 عينه وتمثل نسبه72.7% بينما كانت 45عينه سالبه وبنسبة .27.3%. ان ارتباط الايجابيه المصليه بالفئات العمريه كان عالي المعنويه من الناحيه الاحصائيه((p<0.01وكذلك مع الجنس كان الارتباط معنويا(P < 0.05). استخدم اختبار سلسلة البلمره المنعكس للكشف عن فايروس مرض الحمى القلاعيه من حيث التشخيص الاولي والخاص بتحديد الانماط المصليه.جمعت عينات سريريه مختلفه ممثله لمرض الحمى القلاعيه من الابقار المريضه والتي تشمل الحويصلا ت الفمويه والمصل واللعاب. ان تشخيص الفايروس بالاعتماد على تفاعل سلسلة البلمره المنعكس تم باستخدام البادئات 1F / 1R)و A- 1C5و O-1C272و As1-1C505)وبحزمه متوقعه (328و866و639على التوالي).من ثلاثه وثمانون عينه ضخمت68(81.9%) عينه فقط بواسطة تفاعل سلسلة البلمره المنعكس ولوحظ ان 19(22.9%)عينه منها كانت موجبه للنمط المصلي A- 1C562وان26(31.3%) عينه منها كانت موجبه للنمط المصلي O-1C272وكانت نتائج النمط المصلي As1-1C505 سالبه في جميع العينات.اعتمادا على الجنس لم تكن نسبة تكرار وجود الحامض النووي الفايروسي معنويه احصائيا (P>0.05) بينما كان الفرق بين الفئات العمريه كان معنويا (P < 0.05).

Keywords

Serum --- Bovine --- ELISA.


Article
DIAGNOSIS OF BOVINE ANAPLASMA MARGINALE IN NORTH WESTERN LIBYA USING SEROLOGY AND BLOOD FILM EXAMINATION: A COMPARATIVE STUDY
تشخيص اصابة الابقار بجرثومة Anaplasma marginale في شمال غرب ليبيا باستخدام فحص المسحات الدموية والفحوصات المصليه : دراسة مقارنة

Author: L. S. AL-Bassam*, S. O. AL- Garib**, S.R.EL-Attar***, E. Abdunaser and O. E. Abdouslam
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 119-131
Publisher: Basrah University جامعة البصرة

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Abstract

Anaplasmamarginale (A. marginale) is an obligate intra-erythrocytic rickettsia;it is the cause of anaplasmosis, an important tick-borne disease of cattle. Recovered and vaccinated cattle in endemic areas are apparently normal but remain persistently infected and serve as reservoirs for the parasite.This study intended to detect A. marginale in infected and apparently healthy subclinically infected cattle in North Western Libya. During the period extended from March-2006 till September-2007, blood samples and blood smears were collected from totally 119 adult cow (group – I). These cows were raised at some governmental and private farms in Tripoli, Al-Zawiya and Imssallata districts. Blood smears were stained with May-Grunewald- Giemsa stain and examined under Light microscope to detect the presence of intraerythrocytic bacteria. Indirect- ELISA (IELISA) using a 19 KD A. marginale recombinant antigen was used to detect serologically positive reactors.During the study period, 20 cases of acute anaplasmosis were diagnosed in these farms (Group-II); where, three cows died and two aborted. The Seroprevalence for A. marginale by IELISAwas 64% and 100% in group I and II, respectively. Stained thin blood smears failed to detect infective RBCs in group I, however, variable degrees of parasitaemia were detected in group II.In conclusion, this study approved that serological test (IELISA) was more reliable than direct microscopic examination of stained blood smear in detection of chronic persistent anaplasma-infected cows in endemic areas.


Article
SEQUENCES AND EXPRESSION OF THE ACTIVE LYSOSTAPHIN GENE FROM STAPHYLOCOCCUS SIMULANS ISOLATED OF BOVINE MASTITIS AND ITS BACTERICIDAL EFFECT ON Staphylococcus Aureus
تحديد تسلسل وتعبير جين اللايسوستافين من

Author: Jalal Y. Mustafa
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2012 Volume: 11 Issue: 2 Pages: 321-339
Publisher: Baghdad University جامعة بغداد

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Abstract

Sixty milk samples were recovered from cows suffering from clinical mastitis in Basra. Among these 55 (91.66%) gave good growth in blood agar. After microscopical and biochemical tests, 49 isolates have been diagnosed as G+ bacteria staphylococci. Thirty (50%) isolates of S. aureus are coagulase positive staphylococci (CoPS) and 19 (31.66%) isolates were coagulase negative staphylococci (CoNS). Among the recovered isolates, two isolates are S. simulans and only one gave good antimicrobial activity against S. aureus on the basis of inhibition zone. The active lysostaphin gene with determined size and sequences are 738 bp was isolated and then cloned to E. coli. A lysostaphin gene was prepared with terminal Histidine group in order to isolate and purify lysostaphin protein by used special primers. His-tag column was chosen for isolation and purification of protein with in short time. A high fidelity in running, one band of protein in polyacrylamide gel was seen in comparison with standard protein from Sigma which gave more than two bands, after running in polyacrylamide gel, the molecular weight was about 27 KDa. The antibacterial effect of lysostaphin against S. aureus was studied in Vitro which gave good inhibition zone on sold media. The LD50 of lysostaphin was determined and there were no effects of lysostaphin on mice with all concentrations used. The effect of lysostaphin against S. aureus in Vivo was studied by inducible infection in mammary glands (mastitis). The antibacterial activity of lysostaphin showed significant effect. Finally the histological studies of mammary glands showed the significant activities of lysostaphin to inhibit the growth of S. aureus. To eliminate the antibiotics resistant building this study is conducted. The aims of the study: Isolation and identification of Staphylococcus simulans as a source of lysostaphin producer from mastitis cows. Cloning the lysostaphin gene, using expression of this gene as bactericidal effect on S. aureus in plate. Screening the effect of lysostaphin on induced infected mice (locally) with S. aureus.

جمعت 60 عينة حليب من أبقار مصابة بالتهاب الضرع السريري في محافظة البصرة. وجد إن 55 عينة بنسبة (91.66%) أعطت نمو بكتيرياً على وسط أكار الدم، وبعد التشخيص المجهري والكيموحيوي تم تشخيص 49 عزلة (81.66%) لجراثيم المكورات العنقودية الموجبة لصبغة كرام، 30 عزله (50%) S. aureus موجبة لإختبار التجلط و19 عزله (31.66%) مكورات عنقودية سالبة لإختبار التجلط. حددت منها عزلتان لبكتريا S. simulans ، أعطت واحدة منها فعاليه تثبيطية لبكتريا S. aureus. عزل جين اللايسوستافين الفعال وحدد حجمه البالغ 738 زوج قاعدي، كما تم تحديد تتابعه و كلونته إلى بكتريا E. coli ودراسة تأثير البكتريا المتحولة بالجين الفعال على بكتريا S. aureus . بعد ذلك تم تحضير جين لايسوستافين حاوي على مجموعة هستدين طرفيه لغرض العزل والتنقية بإستعمال برايمرات خاصة ثم إستنساله، عزل بروتين اللايسوستافين بإستعمال عمود الهس تاك الذي أظهر كفاءة عالية جداً بالعزل والتنقية بفترات زمنية قصيرة، وقد ظهر ذلك جلياٌ عند ترحيل البروتين على هلام الأكريل أمايد، إذ ظهرت حزمة واحدة مقارنة مع البروتين القياسي من شركة سكما الذي أعطى أكثر من حزمتين عند الترحيل، وبعد ترحيله على هلام الأكريل أمايد تم تحديد تركيزه و تتابعه وحجمه الذي بلغ حوالي 27 كيلو دالتون. تم إستعمال البروتين المنقى كمضاد بكتيري لبكتريا S. aureus وذلك بدراسة تأثيره القاتل لها خارج النظام الحي فأعطى مقدرة واضحة لقتل بكتريا S. aureus . بعد ذلك تم تحديد الجرعة نصف القاتلة للبروتين المنقى (LD50) وذلك بإصابة 30 فأر، وأشارت النتائج إلى عدم ظهور أي نسبة قتل للفئران مع كل التخافيف المُستعملة دلالة على سلامة الحيوان المحقون بالبروتين المنقى. كذلك تمت دراسة تأثير البروتين المعزول على بكتريا S. aureus في داخل النظام الحي وذلك بإحداث إصابة تجريبية موضعيه وذلك بإحداث التهاب ضرع تجريبي، تمت معالجة الحيوانات المصابة بالبروتين المعزول والمنقى، أوضحت النتائج التأثير الكبير للايسوستافين الذي منع أي تأثير على الحيوان بعد حقنه، كما تم عزل الغدد اللبنية وعمل تقطيع نسيجي لنسيجها، وأظهر بروتين اللايسوستافين فعالية مميزة وواضحة في القضاء على تأثير بكتريا S. aureus المتسببة بإحداث الإصابة.


Article
SEROLOGICAL DETECTION OF ROTAVIRUS INFECTION IN BOVINE AND HUMAN
الكشف المصلي والجزيئي لاصابات الروتافايروس في الابقار والانسان

Author: Enas R. Mohsin, Khalid H. Al-Jaff
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 349-359
Publisher: Basrah University جامعة البصرة

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Abstract

Rotaviruses, causing acute gastroenteritis, that infect humans and animals around the world. There are many assays had been developed for the detection of rotavirus or the viral antigens. The present study was done on 79 samples of stool collected from pediatric patients with acute watery diarrhea aged from one months to 5 years admitted to Basrah Maternity and children hospital in Basrah province, during the period from October 2014 to February 2015. Ninety diarrheic fecal bovine samples were included in this study. All samples were used for the investigation and detection of rotavirus antigen by Enzyme-Linked Immunosorbent assay (ELISA).According to ELISA results, 10 out of 79(12.7%) pediatric stool samples rotavirus antigens were detected in children. Percentage (20.7%) of positive rotavirus antigen were detected in the patients at second age group (>6 months). Followed by 8% of patients at first age group (<6 months) these differences were not significant (P>0.05). The percentage of rotavirus antigen was higher in males patients (16.7%) compared to females (P>0.05) and also the differences were not significant differences (P>0.05). These results of rotavirus antigen detection in 90 diarrheic bovine fecal samples showed that this antigen was excreted by 56.7%of diarrheic calves. Additionally the higher non-significant (P>0.05) excretion percentage according to age was observed in 63.4 % of calves > 1 year old and the lower percentage(51.1%) was observed in the first age group( < 1year) calves old. The differences in sex were not significant (P>0.05) in the percentage of rotavirus antigen detection were also detected as 63.5% of male fecal samples show positive rotavirus antigen excretion whereas only 47.4% of female fecal samples were positive.


Article
PHENOTYPIC STUDY ON THE CAPACITY OF BIOFILM PRODUCTION IN STAPHYLOCOCCUS AUREUS ISOLATED FROM BOVINE SUBCLINICAL MASTITIS AND THEIR IMPACT ON RESISTANCE TO ANTIMICROBIALS
دراسة مظهرية حول قدرة انتاج الغشاء الحيوي للمكورات العنقودية الذهبية المعزولة من التهاب الضرع تحت السريري البقري وتأثيرها على مقاومتها للمضادات الميكروبية

Author: Ali A. Al-Iedani
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 2 Pages: 111-127
Publisher: Basrah University جامعة البصرة

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Abstract

The study was intended for identification and characterization of Staphylococcus aureus isolated from bovine subclinical mastitis cases. A total of 143 milk samples were collected from apparently normal cows from Basrah province. California mastitis test was used to detect 81 (56.6%) samples as subclinical mastitis. However, by using bacteriological and biochemical tests 36(44.44%) isolates were confirmed as S. aureus. Antimicrobials susceptibility assays of isolates revealed that, all of them were completely susceptible to chloramphenicol, gentamycin and vancomycin. Oxacillin and cefoxitin susceptibility illustrated that 22(61.1%) isolates were resistant to methicillin (MRSA) and 14 (38.9%) isolates were methicillin susceptible (MSSA). Phenotypic production of slime and biofilm were evaluated by using Congo red agar and microtiter plate techniques, 31 (86.1%) isolates were slime producer and 29 (80.6%) were biofilm producers from all tested isolates. The production of slime and biofilm of MRSA isolates were 95.5% and 90.9%, whereas, for MSSA were 71.4% and 64.3% respectively. The differences in slime and biofilm production among MRSA and MSSA isolates were statistically significant. All MRSA isolates were resistant to oxacillin, penicillin, cefoxitin, ampicillin and cefotaxime, and these isolates showed low resistance to erythromycin and lincomycin each (9.1%) and doxycycline (18.2%). However, these isolates were susceptible to vancomycin, gentamycin, nitrofurantoin and chloramphenicol. All MSSA isolates exhibited resistance to ampicillin and 85.7% of isolates to cefotaxime, however, all isolates were sensitive to azithromycin, cefoxitin, chloramphenicol, gentamycin, oxacillin and vancomycin. The differences in antimicrobial susceptibility between MRSA and MSSA isolates were highly statistically significant. Results of this study indicate that Staphylococcus aureus is the most important agent of bovine subclinical mastitis, isolates which have resistance to methicillin and produce biofilm have propensity to be multiple antibiotic resistant

Keywords

: Bovine --- S. aureus. --- Methicillin --- MRSA.


Article
Detection of Cattle Foot and Mouth disease Virus by RT-PCR and ELISA

Author: Adnan M. Al-Rodhan
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 319-331
Publisher: University of Kufa جامعة الكوفة

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Abstract

This study was conducted with73 cattle in different region of Basrah governorate with typical clinical symptoms of FMD from April to July 2013 in Basrah ,Iraq. Blood ,saliva and vesicle fluid from the affected animals were collected and tested by ELISA and RT-PCR. Collected sera were tested for antibodies against FMDV using a commercial ELISA. Antibodies to FMDV were detected at an overall prevalence rate of 94.5 %. There was no significant seropositivity ratio variation in relation to sex and age.RT-PCR base detection of FMDV for primary and serotype specific diagnosis was done with( universal primer sets 1F / 1R, A- 1C612 and O- ARS4 ) with expected band of(328 ,865 and1301 bp respectively). Universal primer pair 1F/1R detected FMD in 55 out of 73(75.3%), of these 37 (92.5%) Females and 18 (62.1%) males samples, with high significant statistical difference (p<0.01) between males and females in the PCR positivity ratio and there was no positive samples of FMDV Universal primer in suspect seropositive cases . The tested samples with positive universal primer were amplified with specific primers A-IC612 in 15( 27.3%) and nil for serotype O- ARS4.,. The circulation of Serotype A was higher in the males compared to the females with a ratio of 33.3% (6/18) but this difference was not statisticaly significant( p > 0.05) .The statistical analysis showed high significant differences (P>0.01) between animals that tested for Universal primer in relation to age. Out of all animals that tested positive for Universal primer the age group <1-4 year realized a ratio of 58.6 %while ratio of age group >4-8 year was 95%. Serotype A circulation was not significantly differed (P>0.05) between the tested animals in relation to age with overall ratio 27.3% (15/55) and higher ratio (31.6%, 12/38) was observed in the older animals compare with (17.7%, 3/17) in younger.

Keywords

ELISA --- Rt-PCR --- Bovine --- FMD


Article
Study of Ivermectin effect on the bovine warts

Authors: Thekra A . Ebraheem --- Saba J . Jawad --- Ghassan H . Jameel
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 2 Pages: 117-126
Publisher: Diyala University جامعة ديالى

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Abstract

Cutuneous warts in bovine are benign tumors induced by virus belongs to
papillomaviruses family . Ivermectin drug used in treatment of endo and ectoparasites in
animals and to treat the bovine warts at a dose 300 microgroms / Kg . B . wt . sub
cutaneously .
Ten cattles were be used in this expirement , five of them injected subcutaneously at
the base of the wart and other Five injected also subcutaneously upper of the scapular bone
faraway the wart position .
Statistical analysis for obtained results reveals presence of singnificantly raised
(P<0.05)in the mean of total white blood cells count from (5.480±1.064)cell before treatment
and reach to (7.380±0.511)cell after treatment and the mean of lymphocytes rate from
(35.200±6.685)% before treatment and reach to (65.400±11.929) %after treatment .Also
reveals significant depression (P<0.05) in the mean of neutrophils and monocytes rates from
(57.800±6.685)% , (4.200±0.836)% before treatment and reach to (31.200±11.322)% , (1.600
± 1.140)% after treatment respectively .
Eight cattles were being recover from sum of ten cattles and the clearance rate reach's
to 80% without any side effect on the animals so the mean of total white blood cells counts
for recovered animals are showing in table -1 are being normal was (5.940±0.698) also the
mean of differential percentage of the white blood cells is returned normal , neutrophils was
(60.400±0.270)% , lymphocytes (33.600+3.209)% , monocytes (4.400±0.894)% , eosinophils
(1.200±0. 447)% ,and basophils (0.600±0.548)% .


Article
Detection of Bovine Viral Diarrhea Virus by Conventional RT-PCR: A comparative Study
الكشف عن فيروس الاسهال البقري الفيروسي بواسطة (RT-PCR) التقليدي: دراسة مقارنة

Author: K. S. A. Al-Ajeeli كريم سعدون علي العجيلي
Journal: Al-Anbar Journal of Veterinary Sciences مجلة الانبار للعلوم البيطرية ISSN: 19996527 Year: 2011 Volume: 4 Issue: 2 Pages: 121-128
Publisher: University of Fallujah جامعة الفلوجة

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Abstract

Fifty serum and buffy coat samples were collected from bovine from illegal abattoirs around Baghdad. RNA extraction was performed on buffy coat samples. These samples were subjected to conventional RT-PCR for the detection of BVDV. The result showed that the BVDV was detected by RT-PCR in three samples only. A comparison study was also conducted on the sensitivity of RNA extracted and non-extracted buffy coat and serum samples in conventional RT-PCR. The result showed that RNA extraction is the method of choice when conventional RT-PCR was performed on blood samples from BVDV infected animals. In a final conclusion, BVDV was detected by RT-PCR in local bovine. The findings suggested and proposed epidemiological studies on BVDV in Iraq in conjunction with isolation of the virus and identification of its genotype and biotype

جمع خمسون نموذجا من مصل وخلايا دم ابقار ذبحت في مجازر غير قانونية في المناطق المحيطة ببغداد. استخلص الحمض النووي الريبي من نماذج خلايا الدم ثم عرضت النماذج المستخلصة إلى اختبار(RT-PCR) التقليدي لغرض الكشف عن فيروس الإسهال البقري فيها. واظهرت النتائج وجود الفيروس في ثلاث نماذج فقط. ولغرض المقارنة في تقنية (PCR) لنماذج استخلص الحمض النووي منها وأخرى لم يستخلص الحمض النووي منها تم اختيار 10 نماذج من خلايا الدم تشتمل على الموجبة لوجود الفيروس و10 نماذج مصل لنفس نماذج خلايا الدم العشرة السابقة. استخدمت النماذج في نفس الاختبار ولكن بدون استخلاص الحمض النووي .ظهرت النتائج اكثر وضوحا في حالة استخلاص الحمض النووي مقارنة بنفس النماذج التي لم يستخلص الحمض النووي منها. يستنتج من هذه الدراسة ان فيروس الإسهال البقري موجود في الأبقار في العراق وان استخلاص الحمض النووي من نماذج المصل وخلايا الدم أفضل من عدم الاستخلاص في حالة استخدام (RT-PCR) التقليدي للكشف عن الفيروس. ان النتائج تظهر وجوب اجراء دراسات وبائية عن مدى انتشار مرض الاسهال الفيروسي في الابقار تقترن بعزل الفيروس ومعرفة نوعه البايولوجي والجيني

Keywords

BVDV --- RT-PCR --- bovine viral diarrhea


Article
Detection of Staphyl ococcus aureus capsules producer isolated from bovine and buffaloes mastitis
التحري عن جراثيم المكورات العنقودية المرضية المنتجة للمحافظ والمعزولة من حالات التهاب ضرع الابقار والجامــوس

Author: G. N. Shamoon جورجيت نيسان شمعـون
Journal: Iraqi Journal of Veterinary Sciences المجلة العراقية للعلوم البيطرية ISSN: 16073894 Year: 2006 Volume: 20 Issue: 1 Pages: 39-47
Publisher: Mosul University جامعة الموصل

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Abstract

This study was done for the detection of capsulated Staphylococcus aureus strains which were isolated from bovine & buffaloes Mastitis. 23 (57.5 %) & 8 (53 %) strains of these bacteria were isolated, 6 (26 %) & 2 (13.3 %) were capsule producing strains, and that 9 (39.1 %) & 4 (26.6 %) were slime producing strains, respectively. An increase in the number of capsules and slim producing strains was observed following growing of these bacteria in culture media containing glucose and milk. The experimentally performed histo-pathological study, revealed presence of capsules in lung sections stained with Hematoxylin–Eosin as a halo areas appeared around the bacterial cells. In addition to a strong positive reaction with periodic acid shiffs.

تم في هذه الدراسة التقصي عن سلالات جراثيم المكورات العنقودية المرضية المنتجة للمحافظ والمعزولة من حالات التهاب ضرع الابقار والجاموس اذ تم عزل 23 (%57.5) و 8 (53%) عزلة تابعة لهذه الجراثيم من حالات التهاب ضرع الابقار والجاموس وكانت (% 26) 6 و (% 13.3) 2 عزلة منتجة للمحافظ على التوالي وان (% 39. 1) 9 و (% 26.6) 4عزلة، على التوالي كانت منتجة لمادة Slim. فضلا عن زيادة عدد السلالات المنتجة للمحافظ و Slim بعد تنمية السلالات في الوسط الحاوي على الكلوكوز والحليب. كما أظهرت نتائج الدراسة التجريبية في الجرذان وجود المحافظ في المقاطع النسجية المحضرة من الرئة والمصبوغة بصبغة هيماتوكسلين أيوسين H & E على هيئة هالة حول الخلايا الجرثومية كما اعطت تفاعلا موجبا مع تقنية حمض البريودك شيف Periodic acid shiffs .

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