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Article
Glucose Tolerance in Pregnant Women Inflictedwith Candida Albicans

Authors: Fatma Haider K. AL-Mulla فاطمة حيدر الكلا --- Tarik Hovthy A.T. AL-Khayat طارق مفتي الخياط
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2009 Volume: 5 Issue: 1 Pages: 8-10
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Many previous studies were concerned with the relationship between gestational diabetes and the development of vaginal candidiasis in pregnant women. In this study, our aim is directed to uncover glucose tolerance status in non-diabetic pregnant women inflicted with candida albicans.Methods: Thirty-four pregnant women with vaginal condidiasis (as demonstrated by microscopy) were enrolled in this study. The patients were nearly similar in their anthropometric and demographic criteria with those of the healthy pregnant women (control group, forty –two women). Fasting plasma sugar and glucose tolerance test were alone for all patients and control group.Results: fasting plasma sugar was significantly higher in the candidiasis-positive pregnant women in comparison to those of candidiasis-negative subjects (5.09 mmol/L vs. 4.71, p <0.02). Plasma glucose level after 30 minutes of performing oral glucose tolerance test was also significant (8.47mmol/Lvs. 7.84, P <0.04). The same trend of significance was noticed after 60 minutes and 120 minutes of performing the corresponding test.The results were (8.13 mmol/L vs. 7.10, P <0.02) and (6.90mmol/L vs. 6.15, P<0.05) respectively.Conclusion: the results reveal an impaired oral glucose tolerance test in pregnant women with candida albicans


Article
PCR in Comparison with Culture Methods for The Diagnosis of Candida albicans Responsible for Candidemia in Leukemic Patients
طريقة تفاعل البلمرة المتسلسل في تشخيص تعطن الدم بداء المبيضات البيضاء بالمقارنة مع طريقة الزرع لتشخيص فطر المبيضات البيضاء

Authors: Saba Sabeeh صبا صبيح --- Azhar A. F. Al-Attraqhchi ازهار عبد الفتاح الاطرقجي --- Elham Al-Aswad الهام الاسود
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2013 Volume: 5 Issue: 2 Pages: 29-35
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Currently, candidemia infections represent an increasing cause of morbidity and mortality in seriously ill hospitalized patients. Because the accurate diagnosis of candidiasis remains difficult, fast and reliable assay for characterization of fungal pathogens is critical for the early initiation of adequate antifungal therapy and/or for introduction of preventive measures.Objective: To detect candidemia in leukemic patients by molecular methods in comparing with golden standard method(culture method).Materials and methods: A total of 60 leukemic patients were included in this study. Clinical type and other demographic data were recorded. Blood samples were taken from each patient, culture; germ tube formation and carbohydrate fermentation were done for each sample. DNA extraction and polymerase chain reaction (PCR) were used for detection of Candida albicansin cultured bottles. This study was conducted on leukemic patients admitted to four different hospitals in Baghdad city from September 2010 to March 2011. Sixty patients suffering from acute lymphoid (ALL) and myeloid (AML) leukemia were included in this study. The age of patients were ranging between 3-46 years old. Twenty five apparently healthy individuals were enrolled in this study as control group. Three milliliters of blood were collected from each patient; 1.5 ml was inoculated in 20 ml Brain heart infusion broth (Cruikshank. 1975). The rest of blood (1.5ml) was stored in -20ᴼC for further analysis. Blood cultures were incubated at 37°C for 10 days, and examined daily for growth. DNA purification kit was purchased from QIAGENE®Company. This method was used for the purification of genomic DNA from fresh or frozen samples of 1 ml overnight yeast cultures by using the GentraPuregene Yeast/Bact. Kit. PCR was performed to detect Candida albicans species through the amplification of specific gene (αINT1) Results: Only one positive culture result out of 60 samples was obtained for Candida sp., (1.7%). PCR results showed that there were only three out of sixty were positive for C. albicans (5%). In this study we obtained only one positive sample according to culture, while three samples only gave positive results according to PCR method. These results suggest that molecular analysis of candidemia is more sensitive and less time consuming than culture and other conventional methods.Conclusions: From this study, we concluded the following:1.The rate of candidemia was 1.7% among leukemic patients, according to culture results.2.Only 5% of blood cultures was positive according to PCR.3.PCR results showed 100% sensitivity and 96.6% specificity and it is rapid, easy, reliable and also applicable in clinical laboratory for identification of medically important

حالياً الإصاباتَ بداء المبيضات منتشرةوبنسب متزايدةبالاخص للمرضى الراقدين في المستشفيات. ولأن التشخيصَ الدقيقَ لداءفطرالمبيّضاتِ يَبْقىصعبَ ,لذلك يتطلب اجراء فحوصات سريعة وموثوقة لمعرفة الأسباب المرضية الفطريةِ لغرض العلاج المبكر او لغرض الوقاية.لقد تم شمل 60 مريضا بسرطان الدم في هذه الدراسةِ. اخذت عينات دم مِنْ كُلّ مريض، وتم زراعته بوسط Sabourauds agar الفطري،وتم اختبار تشكيل الإنبوبِ الفطري وإختمارِ الكربوهيدراتِ. تم أستخلاص الحامض النووي الرايبي منقوص الاوكسجين وتفاعل البلمرةالمتسلسل (PCR) لفطر المبيضيات البيضاء.أظهرت النتائج بأنّ هنالك عينةِ دمّ وحيدةِ إيجابيةَ للزرع الفطري. وبواسطة عينات اخذت من القناني المزروعة، كان هنالك 3 عيناتَ ايجابية لأستخلاص الحامض النووي الرايبي منقوص الاوكسجين وتفاعل البلمرةالمتسلسل (PCR) لفطرِالمبيّضات البيضاء. وكانت الطريقة الاخيرة(PCR) هي الاكثر حساسية لكشف داء المبيضيات في الدم .نقترح استخدام طريقة البلمرة في تشخيص مرض اللوكيميا او اي مرض داخلي تسسببه هذه الفطريات بدل طريقة الزرع الكلاسيكبة كونها غير دقيقة نتيجة حدوث التلوث بالجراثيم الاخرى عند زرع عينة الدم كما انها تستهلك موادا كثيرة لان كل عينة يجب ان يعاد زرعها يوميا ولمدة شهر كامل الى ان يتم الحصول على النتيجة النهائية.

Keywords

Candidemia --- PCR --- Candida albicans.


Article
Antifungal effect of honey against Candida albicans isolated from saliva

Author: Maisam Kh. Al-Anii ميسم خضر العاني
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN: 19918941 Year: 2014 Volume: 8 Issue: 2 Pages: 6-8
Publisher: University of Anbar جامعة الانبار

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Abstract

The antifungal activity of different concentration of natural honey , synthetic honey and sugar syrup were examined against ten strains Candida albicans that isolated from saliva of patients with dentil caries and gingival ulcer . The antifungal test was done by agar dilution method by adding honey to the agar after sterilization . The concentrations used in this study of all sample were ranged from (10%-85%) (w/v) The natural honey MIC that inhibited C. albicans growth ranged from (20%-40%)(w/v%) . A synthetic honey and sugar solution was also tested to determine antifungal activity attributable to sugars. C. albicans was more resistant against the presence of synthetic honey and sugar syrup in the culture medium at concentrations above 70% (w/v%) . What the data suggests is that the component in the honey responsible for the observed antifungal in vitro properties is not sugar based. Honey might be tapped as a natural resource to look for new medicines for the treatment of mycotic infections. This could be very useful, onside ring the increasing resistance of antifungal.

Keywords

Antifungal --- Honey --- Candida albicans


Article
ADHERENCE OF CANDIDA ALBICANS TO UROEPITHELIAL CELLS

Authors: Ilham AM Al-Kawaz الهام عبد الواحد الكواز --- Abduladeem YA Al-Barrak عبد العظيم ياسين البراك
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 4 Pages: 364-370
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundUrinary tract infection (UTI) is usually associated with multiplication of microorganisms in urinary tract followed by adhesion of these organisms to the uroepithelial cells which is considered as the first step for colonization and infection. Adhesion of Candida on the epithelium of the urinary tract stands as the first step in the pathogenesis of Candidiasis. The adhesion process is under the impact of many factors.ObjectiveThis study was designed to determine the susceptibility of uroepithelial cells obtained from patients with different urinary tract diseases, for adherence with Candida albicans in vitro.MethodsForty eight urine samples collected from women attending Medical City Hospital-Baghdad suffering from urinary tract diseases and proved to have Candida growth. Ten urine samples were collected from apparently healthy age matched females and used as a control. The uroepithelial cells of each sample have been collected and incubated with Candida albicans cells and the percentage of uroepithelial cells with an adhered Candida cells (% EC). The mean number of Candida/epithelial cells (C/E) was estimated before and after adherence assay.ResultsKlebsiella infection showed the highest percentage of uroepithelial cells with Candida (%EC), next in frequency was samples with high number of uroepithelial cells, then, Streptococcus pyogenes, Escherichia coli, pus cells, and finally samples with high number of RBCs. This study indicates that receptivity of Candida adhesion to the uroepithelial cells of urinary tract have different affinity depending on the type of modulating factor surrounding these uroepithelial cells.ConclusionFrom the obtained data it can be concluded that urine findings including bacterial growth might modulate epithelial cell surface and increase their receptivity for Candida adherence.Key wordAdherence, Candida albicans, uroepithelial cells.


Article
A Novel Approach to Overcome Adherence Property of Candida albicans

Author: Shama M.J. Saadaldin
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2011 Volume: 14 Issue: 3 Pages: 115-118
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The study is aimed to introduce a procedure that mimic the adherence of microorganisms to the plastic tube and to assess the suitable solution to irrigate these microorganisms. Candida albicans broth was inoculated into a 30 cm plastic tube for a certain period of time to allow the adherence of microorganism to the inner surface of tube. Tubes were irrigated with distilled water, tap water or different concentrations of sodium chloride (0.1-0.9%). The results showed that the percentage of Candida albicans which adhered to the inner surface of tube ranged between 0.27% and 30% of the initial inoculum. Tap water was superior than distilled water in removing the adherent Candida. Sodium chloride in concentrations 0.3%, 0.6% and 0.9% completely removed the attached Candida while the other concentrations showed inconsistent results. It concludes that irrigation with water or certain sodium chloride concentrations, which interfere with the fluidity of Candida albicans, is the simple and best method to eliminate the adherent microorganisms

: هدفت الدراسة الى ايجاد طريقه تماثل التصاق فطر المبيضات على بطانة الانابيب البلاستيكية وايجاد افضل المحاليل لازالة التصاق هذه المبيضات.طريقة العمل: تم تمرير مرق فطر المبيضات في انبوب بلاستيكي بطول 30 سنتمتر لمده معينة من الزمن سمح لهذه المبيضات الالتصاق على جدران بطانة الأنبوب. تم غسل الانابيب بالماء المقطر و ماء الحنفيه وبتراكيز مختلفه من كلوريد الصوديوم بنسب تراوحت بين 0،1% و0،9%. تم مراقبة الكثافة البصرية لمرق فطر المبيضات وكذلك محاليل سقي الأنبوب بوساطة مقياس الطيف الضوئي عند طول موجي 620 نانوميتر.النتائج: اظهرت النتائج ان نسبة المبيضات التي التصقت على بطانة الانبوب البلاستيكي تراوحت بين 0.27 و 30% من الجرعة الملقحة. كان سقي ماء الحنفيه افضل من الماء المقطر في ازالة المبيضات الملتصقة، كما لوحظ ان سقي كلوريد الصوديو (تركيز 0.3 % و 0.6% و 0.9 %) أزال المبيضات الملتصقة في جميع النماذج بينما اظهرت التراكيز الاخرى نتائج متباينة. كما واظهرت النتائج انه لا توجد علاقة بين قابليه سقي المحاليل في ازالة التصاق المبيضات وبين جرعة المبيضات الملقحة. الاستنتاج: نستنتج من هذه الدراسه ان طريقة السقي بالماء أو بتراكيز معينة من كلوريد الصوديوم الذي يتداخل مع سيولة المبيضات هي افضل وابسط الطرق لازالة التصاق فطر المبيضات.


Article
Isolation and Identification of Fungi Associated with Otomycosis
عزل وتشخيص الفطريات الملازمة لالتهاب الاذن الخارجية

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Abstract

Objective and Background: Laboratory diagnosis of predisposing factors of otomycosis which represent a significant percentage of clinical external otitis and is usually caused by Candida, Aspergillus; penicillium, clinical symptoms such as otorrhea, erythematic and stenosis of the external auditory canal.Patients: Seventy-five patients were included in this study suffering from symptoms indicating otitis externa in Al-Yermouk teaching hospital, E.N.T. department during the period from November 2007 to April 2008.Methods: The specimens were taken by sterile cotton swabs from bony portion of the external ear. All specimens were cultured on sabouraud Dextrose Agar with chloramphenical (0.05mg/ml). Mycological Cultures were incubated at (25-27) ċ for 2-3 weeks and examined microscopically every 3 days.Results: In this study 75 patients (44 Female and 31 male) with symptomatic otomycosis were confirmed by direct microscopy and cultures. Results revealed that (45) 60% of patients (38 female and 7 male) showed association of fungi and Candida albicans. Female susceptibility to fungal infection was more than male 82.23%. The most common fungal pathogens isolated from ear were Aspergillus niger (44.44%) followed by Candida albicans (33.33%), and penicillium spp. (22.22%).Conclusion: In clinical finding of otitis externa, mycological examination could be very important in setting accurate diagnosis and appropriate therapy can be decided. In this study we found that otomycosis is common in female than male and A. niger is the major etiologic agent in ear infection.Keywords: Otomycosis, Aspergillus, Candida albicans, penicillium.

الهدف: هو التشخيص المختبري للفطريات المسببة لالتهاب الاذن والذي يشكل نسبة مهمة من التهاب الاذن الخارجية والمتسببة عن الفطريات وهي المبيضات المرضية Candida وAspergillus وPenicillium والاعراض السريرية بشكل عام وهي قيح جاري من الاذن واحمرار وانسداد الاذن الخارجية.طرق العمل : تضمنت الدراسة 75 مريض يعانون من اعراض تشير الى التهاب الاذن الخارجية في مستشفى اليرموك التعليمي وحدة الانف والاذن والحنجرة وفي الفترة من تشرين الثاني 2007 الى نيسان 2008. تم اخذ العينات بأستخدام مسحات قطنية معقمة من افرازات وقيح الاذن الخارجية وتم تنمية العينات على وسط Sabourad Dextrose Agar مع اضافة المضاد الحيوي البكتيري الكلورامفينكول وبتركيز (0.05 ملغم امل)و تم حضن المزارع الفطرية بدرجة (25-27)م ولفترة 2-3 اسابيع وتم فحصها مجهريا كل ثلاثة ايام.النتائج: في هذه الدراسة اثبت التشخيص المجهري والزرعي ول75 مريض اصابتهم بأعراض التهاب الاذن الفطري. لقد اظهرت النتائج ان 45( مريض بنسبة 60%) (38 اناث و7 ذكور) وملازمة واضحة للاصابة بالفطريات و(المبيضات المرضية Candida albicans ). كانت حساسية الاناث للاصابة بالفطريات اكثر من الذكور.Aspergillus niger كان اكثر الفطريا الممرضة شيوعا وعزل بنسبة 44,44% يليها (Candida albicans) بنسبة 33,33% ثم Penicillium spp (22,22%).الاستنتاج: ان التشخيص السريري للاتهاب الاذن الفطري يحتاج الى التأكيد بالتشخيص المختبري للفطريات مما يعطينا تشخيص دقيق ويساعد على تقرير العلاج المناسب. في هذه الدراسة وجد ان التهاب الاذن الفطري في النساء اكثر من الرجال وان A. niger هو من المسببات الرئيسة لالتهاب الاذن الفطري.


Article
STUDY THE INH INHIBITORY EFFECT OF Lactobacillus acidophilus ISOLATED FROM YOGHURT AS PROBIOTICS ON Candida albicans GROWTH IN VITRO AND IN VIVO
دراسة التأثير التثبيطي للعصيات اللبنية المعزولة من اللبن الرائب كمعززات حيوية للحد من نمو خميرة Candida albicans خارج و داخل الجسم الحي

Author: بشرى جاسم محمد
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 2 Pages: 167-179
Publisher: Baghdad University جامعة بغداد

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Abstract

This research was designed to study the inhibitory effect of Lactobacillus acidophilus which has been isolated from yoghurt as probiotics in reduction of Candida albicans growth in vitro and in vivo. The results showed that the Minimum Inhibitory Concentrations and Minimum Fungicidal Concentrations were 60% and 70% respectively, also the inhibition zone of L. acidophilus against C. albicans reached to 26mm in solid medium .The out come of in vivo study (histological examination ) clarified that C. albicans caused clinical pathological effect in mice tissue organs ( liver , intestine, stomach , kidney) when administrated orally by 1.5x 10 8 cfu ml C. albicans that effect decrease by orally inoculated with same dose of L. acidophilus.The results reflect the ability of L. acidophilus to reduce certain clinical pathological change in mice organs, with promising encourage to use the L. acidophilus as biotherapeutic agents against C. albicanas infections.

تضمن البحث دراسة التأثير التثبيطي للعصيات اللبنية المعزولة من اللبن الرائب كمعززات حيوية للحد من نمو خميرة Candida albicans خارج و داخل الجسم الحي. أوضحت النتائج إن التركيز المثبط الأدنى والتركيز القاتل الأدنى كان % 60و%70 على التوالي بينما وصل القطر التثبيطي للعصيات اللبنية ضد هذه الخميرة إلى 26 مليلتر على الوسط الصلب . بينت النتائج عند إجراء الدراسة داخل الجسم الحي( الفحص النسيجي) أن خميرةC. albicans سببت تأثيرات مرضية واضحة في أنسجة الأعضاء (كبد, أمعاء, معدة, كلية) عندما جرعت الفئران بمقدار 0.1 مليلتر 1.5x 10 8) خلية حية (تناقص هذا التاثير عند تجريع الفئران بذات الجرعة من العصيات اللبنية, مما يعكس قابلية العصيات اللبنية في التقليل من التغيرات المرضية في أنسجة الفئران وهذه النتائج تشجع على استعمال هذه العصيات كعلاجات حيوية للحد من التأثيرات المرضية لخميرة C. albicans.


Article
Antifungal Effect of Some Medicinal Plant Extracts On Candida Albicans Adherence on Acrylic Resin Denture Base Material. An In Vitro Study

Author: Tarik Y.Kassab -Bashi
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2014 Volume: 14 Issue: 28 Pages: 139-144
Publisher: Mosul University جامعة الموصل

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Abstract

Evaluate the antifungal effect of some medicinal plant extracts (peppermint, rue, pomegranate and garlic) and their anti–adherent effect on C. albicans cells that attached on the fitting surface of acrylic resin denture base. Materials and Methods: This study evaluated the antifungal effect of plant extracts, using broth micro dilution method at two concentrations for each one. The visualization, in-spection and enumeration of adherent C. albicans cells and the detection of the anti–adherent effect of these plant extracts was achieved by using fluorescent microscope. For each plant extract, two concen-trations in addition to the time of immersion at three different intervals (1 h, 24 hrs and 48 hrs) were evaluated for the anti–adherent effect. Result: Antifungal effect of plant extracts on candida albicans cells, was measured by spectrophotometer which showed that both rue and pomegranate extracts had the best antifungal effect at first concentration, while in the second concentration, pomegranate and garlic extracts had the best antifungal effect . The mean number of remaining C. albicans cells which adhered on acrylic resin samples after treatment by immersion in the first concentration of plant ex-tracts for one hour was (3.1), (3.7), (3.7) and (4.7)] cells /mm2 respectively; while after treatment with second concentration of plant extracts for one hour was [ (4), (4.4), (4.4) and (4)] cells / mm2 respec-tively for the above mentioned plant Conclusion: All examined medicinal plant extracts were signifi-cantly effective in dislodging C. albicans cells from acrylic resin samples at their different concentra-tions and statistically there was no significant difference among the different times of immersion.


Article
Neomycin sulfate: A new antifungal and antiadherent agent against Candida albicans cells on heat–cured acrylic resin denture base material. An in vitro study

Authors: Ghada Y Abdul–Rahman --- Nagham H Kassab
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2003 Volume: 3 Issue: 6 Pages: 139-142
Publisher: Mosul University جامعة الموصل

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Abstract

Neomycin sulfate is an antibacterial agent. In this research, the antifungal and antiadherent effect of neomycin sulfate on Candida albicans that colonize the heatcured acrylic resin denture base material was evaluated. The results showed a significant effect compared with the control group. This effect is similar to the effect of the known antifungal agents (fluconazol and chlorhexidine) and lesser than (nystatin).


Article
The effect of some antifungal agents and chlorhexidine on Candida albicans adherence on acrylic resin denture base surface (In vitro study)

Authors: Nagham H Kassab --- Ghada Y Abdul–Rahman --- Tariq Y Kassab–Bashi
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2004 Volume: 4 Issue: 7 Pages: 65-72
Publisher: Mosul University جامعة الموصل

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Abstract

The adherence of Candida albicans on acrylic resin denture base surface is considered as the first step in the pathogenicity of candida–associated denture stomatitis which is the most prevalent form of oral candidiasis in denture wearers. This study was an in vitro study aimed to evaluate the anifungal effect of fluconazole, nystatin and chlorhexidine and their antiadherent effect on Candida albicans cells that attached on the fitting surface of denture base. It was determined, using broth microdilution method at two concentrations for each drug, and the results were determined using spectrophotometer. All drugs had a highly significant antifungal effect on Candida albicans isolate at their two concentrations. The effect of saliva on the adherence of Candida albicans on the fitting surface of denture base was also considered. This study concluded the importance of saliva in increasing the microbial adherence; i.e., the mean number of adherent cells on acrylic resin samples in the absence of saliva was 7.4 + 1.9 cell/mm2 and with the presence of saliva the number was 29.1 + 2.7 cells/mm2. The number of remaining adherent cells was counted using fluortescent microscope. The mean number of remaining cells which adhered on acrylic resin samples after the immersion in first concentration fluconazole, nystatin and chlor-hexidine for one hour respectively were 4, 5 and 2.6 cells/mm2 and for the second concentration/1 hour the numbers were 3.2, 4.1 and 1.7 cells/mm2 compared to the normal number of adherent cell 29.1 + 2.7 at p<0.01.

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