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3.THE EFFECTS OF DEXAMETHASONE ON TIBIA DEVELOPMENT OF LOCAL CHICK- EMBRYO. I: COMPUTER-ASSISTED MORPHOMETRIC STUDY

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Abstract

Background:Dexamethasone is a glucocorticoid as a member of the steroidal anti-inflammatory and immunosuppressant. It has well documented effects on skeletal structures osseous and cartilaginous, commonly used to treat or control diseases.Objective:To evaluate by histomorphometric study the effects of dexamethasone on the embryogenesis of long bones in chick embryos.Methods:Forty-eight fertile chick eggs of Gallus gallus domesticus, were used. The eggs were divided into 2 groups; control and treated groups of 24 eggs each, these groups were subdivided into 4 subgroups (n=6 eggs). On day 10 of incubation, the control group was injected with 25 µl of distilled water while the treated group was injected with 25 µl of distilled water contained 8 µg dexamethasone. In the next days (11, 12, 13, and 14 of incubation), 12 chick embryos were sacrificed in each day. A computer-assisted morphometric/ image analysis (Motic Image Plus version 2.0ML), was used to measure length, area, perimeter of tibiae, and the area and perimeter of the perichondral osseous collar of cross section in mid-diaphyseal zone of these bones.Results:These bones of chick embryos treated with dexamethasone, suffered shortening and retardation in length, weight, area and perimeter throughout the period of this study, decline area and perimeter to the perichondral osseous collar in the mid-diaphyseal zone.Conclusion:Dexamethasone given at day 10 of incubation caused tibial bones growth retardation at development stages 11, 12, 13, and 14-days; this was observed in the measured parameters: bone length, area, perimeter and weight.Keywords:Bones, chick-embryo, dexamethasone, histomorphometryCitation:Wathiq Q.S. Ali, Mohammad O. Selman, Insaf J. Al-Hasson, Imad M. Al-Ani, Anam R. Al-Salihi. The Effects of dexamethasone on tibia development of local chick-embryo. I: computer-assisted morphometric study. Iraqi JMS. 2017; Vol. 15(2): 116-128. doi: 10.22578/IJMS.15.2.3


Article
Early Embryology of the Prechordal Region

Author: M. Hayawi Hammash
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2005 Volume: 47 Issue: 1 Pages: 68-72
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Prechordal plate is important midline structure in the head region. It has been shown that it plays a bivotal role in the development of the brain and the eyes.Aim of study: There is confunsion, in the literatures and textbooks concerning of terms: prechordal plate, prechordal mesoderm and the Buccopharyngeal membrane. The aim of this work is to study is based on histological changes of the early stages of the chick embryo hoping to illuminate these aspects especially after the recent revival of attention to the importance of this region in the process of induction of the brain region of the newral tub & the development of the prosencephalon & the eyes as shown by genes activities & expression in the prechordal plate ..Materials & Methods :this study is based on histological description of the early stages (4- 7H.&H.) of the chick embryo to illuminate the nature of these structures . It appeared that the cephalic foregut in the early stages is lined by tall endodermal cells that form three regions: Dorsal wall (PI) where it forms the prechordal plate and contributes to the formation of the prechordal mesodermal mass (mesoendoderm); the rostral end (P2), and the ventral wall (P3) where it fuses with the surface ectoderm forming the oral plate (or the Buccopharyngeal membrane). The contribution of endoderm to the head mesenchyme is emphasized in this study.Conclution: this study indicated that the prechordal plate is not simply the Buccopharyngeal membrare it forms an early proliferating zone , rostal to the notochord , that contribute to the mass of prechordal mesoderm & another zone , which is extending later as a Sessels pouch , which also proliferate for some time . also the ventral extension is a part of Buccopharyngeal membrane.


Article
The use of the hen،s Egg test – chorioallantoic membrane (HET- CAM)test as a novel in vivo model to study the effects of dexamethasone on a differential white blood cell counts
استخدام الغشاء اللقانقي المشيمي لأجنة بيض الدجاج المخصب كنموذج داخل الجسم الحي لدراسة تأثير الديكساميثازون على صورة الدم

Authors: M. Kh. Shindala محمد خالد شندالة --- M. B. Al-Mashhadany محمود بشير محمود المشهداني
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2013 Volume: 12 Issue: 1 Pages: 27-31
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The aim of the present study was to investigate the possibility to use the hen،s Egg test – chorioallantoic membrane (HET- CAM)test as a novel in vivo model to study the effects of dexamethasone on a differential white blood cell counts.The result revealed that, the blood smeared of chick embryos in age 13- 15 days of incubation had to the all types of mature leukocytes . Because of the short period of chick embryos were treated with dexamethasone (90 minutes ) only Showed did not significantly changes in leukocyte counts (lymphocyte, Heterophil, Monocyte, Esinophil and Basophil) in treated groups with defferint doses of dexamethasone ( 2 , 4 , 8 , 16 , 32 , 64 , 128 mcg gram of total egg weight, topically). We conclude for our study that, the (HET- CAM) test can be used as in vivo model to study the effects of dexamethasone on deferential leukocyte counts ,because of the blood smear have all types of leukocyte, also this study manifested this model have low cost accompanied with other laboratory animals.

تهدف دراستنا الحالية الى امكانية استخدام أختبار الغشاء اللقانقي المشيمي لاجنة بيض الدجاج المخصب كنموذج داخل الجسم الحي لدراسة تاثير عقار الديكساميثازون على العد التفريقي لخلايا الدم البيض . اظهرت نتائج دراستنا الحالية احتواء المسحة الدمويه من اجنة بيض الدجاج وبعمر 13-15 يوم من الحضن على جميع انوع خلايا الدم البيضاء الناضجة () . وبسب فترة الحضانة القصيرة لاجنة بيض الدجاج المخصب بعد المعاملة بالديكساميثازون والتي كانت 90 دقيقة فقط لذلك لم يلاحظ حدوث اي فروقات معنوية في اعداد خلايا الدم البيضاء (الخلايا اللمفية والخلايا الهيتروفيل والخلايا الحمضات والخلايا القعدات والخلايا وحيدة النواة) بين المجاميع المعاملة بالجرع المختلفة بالديكساميثازون (2 ،4 ،8 ،16 ،32،64 ،128 مايكروغرام/غرام من وزن البيضة ، موضعيا), نستنتج من دراستنا الحالية امكانية استخدام الغشاء اللقانقي المشيمي كنموذج داخل الجسم الحي لدراسة التغيرات في العد التفريقي لخلايا الدم البيضاء وذلك لاحتواء المسحة الدموية على جميع انوع خلايا الدم البيضاء لاسيما ان تكاليف هذا النموذج منخفضة مقارنة بالحيوانات المختبرية .


Article
Isolation of Infectious Bronchitis Virus in Primary cells of the Chick Embryo Chorioallantoic Membrane
عزل فايرس التهاب القصبات المعدي في الخلايا الابتدائية لغشاء اللقانقي المشيمي من اجنة البيض

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Abstract

The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells. Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 72 hours post-infection in third passage, the cytopathic effect was characterized by rounding up of cells, monolayer detachment, intracytoplasmic brownish colouration was readily observed by immunoperoxidase from 24hours p.i in third passage, and at all times the extracted viral RNA from IBV-infected monolayers was demonstrated by RT-PCR. Tissue culture ineffective dose50 (TCID50) was used to measure virus titration performed on primary chick embryo chorioallontoic membrane cells and the titre in twenty passage was 108.6 TCID50/ml.The results obtained in this study suggested that the primary chick embryo chorioallontoic membrane cells can be used for adaptation infectious bronchitis virus (IBV) and may be considered a step forward for the use of these cells in the future for IBV vaccine production

تم في هذه الدراسة تقييم حساسية الخلايا الابتدائية المعزولة من الغشاء اللقانقي المشيمي لاجنة البيض وحقنها بفايروس التهاب القصبات المعدي. وقد استخدمت كل من المعاير التالية التاثير الخلوي واختبار انزيم فوق الاس الهيدروجين المناعي غير المباشر واختبار سلسلة تفاعل البلمرة (RT-PCR) لتقييم اصابة الخلايا بالفايرس. وبعد 72 ساعة من الاصابة في التمرير الثالث تميز الاعتلال الخلوي للخلايا المصابة بانها اصبحت دائرية اضافة الى تحررها وطوفانها اما في اختبار انزيم فوق الاس الهيدروجين المناعي فقد تميز بظهور اللون البني في السايتوبلازم بعد 24 ساعة من الاصابة في التمرير الثالث في حين اظهر اختبار سلسلة تفاعل البلمرة نتيجة موجبة ولجميع التمريرات. واظهرت النتائج ان هذه الخلايا يمكن ان تستخدم لعزل وتضعيف الفايروس كما يمكن ان تستخدم في تحضير اللقاح في المستقبل.

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