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Article
Potential Healing Effect of Topical Stem Cell Transplantation and Methandrostenoloneon in Induced Cutaneous Wounds in Dogs

Author: Oday K. Luaibi 1 , Laith K.T.AL-Ani 2 , Zena, M. Fahmi 3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 1 Pages: 12-21
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was done to explore the influence of stem cells on cutaneous wounds in comparison with treatment with methandrostenoloneon drug. Cutaneous wound was induced on the back of each dog using disposable dermal biopsy punches . Twenty five dogs were divided into five groups: 1stgroup (T1) was control. 2nd group (T2) was induced without any treatment. 3rd group (T3) was induced wound and treated with phosphate buffer saline, 4rth group (T4) was induced and treated with 5mg/kg/B.W. of Methandrostenolone, 5th group (T5) was induced and treated with stem cells 1x106cell/250 µL of phosphate buffer saline. After 7 and 15 days post treatment level of Hb and RBCs count in T2 and T3 group were decline as compared with control group. The WBCs, Alkaline phosphatase , acid phosphataseas in T2 and T3 showed enhance as contrast with T1. After 7 days, Hb and RBCs in T4 and T5 showed important increase as compared with T2. Moreover the WBCs, alkaline phosphatase, acid phosphatase and total protein levels significantly elevated in the T4 and T5 as compared with T1. After 15 days of treatment the Hb, RBCs, levels of Alkaline phosphatase, acid phosphataseas and total protein levels in T4 showed significant increase as compared with T1, The Hb, RBCs, levels of Alkaline phosphatase, acid phosphataseas and total protein in T5 showed no significant changes as compared with control group (T1). The histopathological section in (T2) explained less collagen and more inflammatory cells, the section of wound tissue in (T4) appeared incomplete healing wound tissue and presence little inflammatory cells, the histopathological section of wound treated in (T5) showed return the wound to normal tissue. From this result it can be concluded that the MSCs have ability to access healing of the wound in comparison with methandrostenolone without any side effect.


Article
Role of acellular bovine urinary bladder submucosa on skin wound healing in Iraqi goats
دور غشاء تحت المخاطية للمثانة البولية البقرية في شفاء جروح الجلد في الماعز العراقي

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Abstract

This study is designed to assess the effectiveness of bovine urinary bladder submucosa on healing of cutaneous wound in Iraqi goats. A 32 (2X2) cm of full-thickness cutaneous wounds were induced in eight goats, two on each side of the lateral thoracic region. The wounds were divided into two equal groups (16 wounds/ group); treatment group included the wounds on the right side which were treated by covering the wound beds with strips of acellular sterilized bovine urinary bladder matrix. While, the wounds on the left side were left without any treatment (control group). The results were evaluated clinically (along) and histopathologically on 7, 14, 21, and 35 days postinducing of wounds. The clinical evaluation of treated wounds showed that the wound healing process contraction%, re-epithelization % and total wound healing % were P<0.05 significantly than that of control wounds at 35 days of the study. The histopathological results confirmed that urinary bladder matrix treated wounds have enhanced cellularity, increased vasculature, thick and large granulation tissue suggesting enhanced cutaneous healing, than those in untreated wounds. Depending on the clinical and histopathological findings, this study concluded that a cellular bovine urinary bladder matrix play an important role in stimulation of cutaneous wound healing of goats without signs of immuno-rejection

ص ممت هذه الدراسة لتقييم دور غشاء الطبقة تحت المخاطية للمثانة البولية البقرية في شفاء الجروح الجلدية في الماعز العراقي . است . سم في الجانب الوحشي للمنطقة الصدرية 2×2 جرح مربع الشكل بقياس 22 حدث ق س مت الجروح إلى مجموعتين مجموعة المعالجة والتي شملت على الجروح في الجانب الأيمن للمنطقة الصدرية والتي ت ،) جرح/مجموعة 11(متساويتين عول ج بتغطية الجروح بشرائط غشاء المثانة البولية البقرية المنزوعة الخلايا، في حين تركت الجروح في الجانب الأيسر بدون علاج (مجموعة سيطرة .) تم تقييم نتائج الدراسة سريريا طيلة مدة التجربة ونسجيا مرضيا ( و 14و 7 )يوما بعد استحداث 34و 21 الجروح .أظهرت المتابعة السريرية أ ن معدلات التقلص، إعادة تكون الخلايا الظهارية وشفاء الجرح الكلي كانت مرتفعة وبفارق مقارنة بالجروح غير المعالجة في مجموعة السيطرة طيلة مدة الدراسة .في حين اثبتت المتابعة النسجية (P>0.05) إحصائي المرضية وجود تحسنا ملحوظا في التفاعل الخلوي، التجهيز الوعائي الدموي وكثافة النسيج الحبيبي في جروح مجموعة المعالجة مقارنة بجروح مجموعة السيطرة . استنادا إلى نتائج المتابعة السريرية والنسجية المرضية استنتجت الدراسة أن غشاء تحت المخاطية للمثانة البولية البقرية قد ى

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