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Article
Diagnostic Approach of Atypical Cells in Effusion Cytology Using Computerized Image Analysis

Author: Zainab A J Al-Obaidi
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2007 Volume: 4 Issue: 1 Pages: 60-62
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Cytology is one of the important diagnostic tests done on effusion fluid. It can detect malignant cells in up to 60% of malignant cases.
The most important benign cell present in these effusions is the mesothelial cell.
Mesothelial atypia can be striking andmay simulate metastatic carcinoma. Many clinical conditions may produce such a reactive atypical cells as in anemia,SLE, liver cirrhosis and many other conditions.
Recently many studies showed the value of computerized image analysis in differentiating atypical cells from malignant adenocarcinoma cells in effusion smears. Other studies support the reliability of the quantitative analysisand morphometric features and proved that they are objective prognostic indices.
Methods: Sixty three cases of pleural and peritoneal smears, previously reported as benign (19) cases, malignant (21) cases or atypical (23) cases, were retrieved from the files. In each of these smears; nuclear area, perimeter, and roundness coefficient of 80-100 cell were determined at x400 magnification by the use of image analysis system.
Statistical analysis was performed using analysis of variance and Tukey's HSD test.
Results: The mean values of nuclear roundness, nuclear perimeter and nuclear area vary between the three groups (benign, atypical and malignant cells) by using analysis of variance (p > 0.01).
The value of nuclear roundness, perimeter and area did not differ significantly between benign and atypical cells (Tukey’s test: p<0.01).
On the other hand, the value of nuclear roundness, perimeter and area showed a significant difference between malignant and atypical cells(Tukey's test: p> 0.01).
Conclusion: In conclusion, our data suggest that cytomorphometry performed on effusion smear cells may provide important information for the differentiation of atypical cells from malignant cells, in which the values of atypical cells are closer to those of benign cells during the examination of pleural and peritoneal smears by the use of image analysis system.
Key words: Effusion cytology, Atypical cells, Cytomorphometry


Article
Oral signs and symptoms and hyperglycemic status of type II diabetic patients in relation to cytomorphometric findings of gingival and buccal cytobrush smears

Authors: Mohammad T. Baban محمد بابان --- Balkees T. Garib بلقيس غريب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 59-65
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Brush cytology is an accepted technique that gets renewed interest. It is now used as an aid for thediagnosis and observation of possible epithelial changes that could be associated with oral mucosal diseases. Thisstudy aimed to evaluate the cytomorphometric changes in gingiva and buccal mucosa of type II diabetics and toassess their relation to oral symptoms and glycemic status.Materials and methods: Cytological Papanicolaou stained smear were prepared from cheek and gingiva of 20 nontreated cases, 20 treated diabetics and 20 healthy persons of both sex after measuring their HbA1c and recordingtheir oral symptoms. Hundred unfolded epithelial cells were evaluated qualitatively using MCID software to measurenuclear and cytoplasmic areas, ratio, perimeters, and form factors. Different statistical analyses were used todetermine relations between studied parameters.Results: Diabetics smears showed large nucleus, small cytoplasm, and small cytoplasm/nucleus ratio compared withhealthy persons with no sex variation. Xerostomia and burning sensation were significantly correlated with nuclearparameters, while HbA1c was significantly correlated with both cytoplasmic and nuclear parameters. Well-controlledpatients showed reduction in nuclear area, but nucleus and cytoplasm form factors were unlike normal.Conclusions: Oral cytology from type II diabetics is associated with detectable cytomorphometric changes that isbetter demonstrated in buccal than gingival mucosa and tend to return partially to their normal values in wellcontrolledpatients, with no sex variation. NA seems to be the main parameter that changed during hyperglycemiaand xerostomia, while both NA and CA were related to burning sensation

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