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Article
PREVALENCE OF Listeria monocytogenes IN FROZEN FISH IN BASRAH CITY MARKETS
تواجد جرثومة Listeria monocytogenes في الأسماك المجمدة في أسواق مدينة البصرة

Author: Ghazi M. Jabir Al- Maliki غازي مالح جابر
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2010 Volume: 9 Issue: 2 Pages: 127-132
Publisher: Basrah University جامعة البصرة

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Abstract

ABSTRACT The present study was performed on 80 samples of frozen fish . The samples were collected from different shops in Basrah city . Listeria spp. Was isolated (13%) of frozen fish , also they were counted in the examined samples. The mean counts were 8.75 x 102 CFU/g. of the fish . L. monocytogenes differentially identified from other Listeria species and could he isolated at (5%) in frozen fish . The drug susceptibility characterization of L. monocytogenes cleared that all isolates (100%) were resistant to Ampicilline and Gentanicine . while the other used antibiotics showed different degrees of antimicrobial sensitivity reactions for streptomycin , Tetracycline , Rifampicine and cefotexim

جمعت ثمانون عينة من الاسماك المجمدة من محلات مختلفة لبيع الأسماك في البصرة ,تم عزل وعد جرثومة اللستريا وبنسبة 13% وانت نسبة اللستريا مونوسايتوجينز 5%,طبق فحص اختبار الحساسية الدوائية على هذه الجرثومة أن جميع العزلات (100 ٪) مقاومة للامبسيلين والجنتامايسين, بينما اظهرت غيرها من المضادات الحيوية المستخدمة درجات مختلفة من ردود فعل الحساسية لمضادات الميكروبات الستربتوميسين ، التتراسيكلين ،والسيفوتوكسين والرافامبسين.


Article
DETERMINATION OF SOME TOXIC TRACE ELEMENTS OF FRESH, FROZEN AND CANNED FISH SPECIES
تقدير بعض العناصر النزرة السامة في عضلاث أنواع من الاسماك الطازجة والمجمدة والمعلبة

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Abstract

In this study, fresh fish (Otolithes ruber, Saurida tumbil, Pseudorhombus malayanus, Acanthopagrus latus, Hypophthalmichthys molitrix and Tenualosa ilisha), imported frozen fish (Megalaspis cordyla, Ctenopharyngodon idella, Labeo rohita and Acanthopagrus latus) and imported canned fish species (sardines, mackerel and tuna) were used to detect and determine some toxic trace elements. All fish samples contained trace elements. The total concentrations mean for Cu, Zn, Pb, Ni, Co, Cd and Mn were between (1.73-11.93), (8.75-25.80), (ND-3.19), (0.11-0.31), (0.10-0.35), (0.23-3.00) and (0.11-1.86) mg/kg dry weight respectively in muscles of fresh fish, and between (13.21-53.74), (6.61-43.93), (0.35-2.10), (0.37-2.30), (0.20-0.54), (1.50-3.53) and (0.13-4.50)mg/kg dry weight respectively in the muscles of frozen fish species, and between(21.75-56.30), (5.21-82.84), (0.25-1.51), (0.33-1.96), (0.20-0.47), (0.51-2.22) and (0.13-0.81) mg/kg dry weight respectively in canned fish . Concentrations average of some trace elements were exceeded the permitted legal limits that set by the Food and Agriculture Organization and the World Health Organization.


Article
Glimpse on Hemostatic Changes Produced By Plasmapheresis

Authors: Haider H. AL-Shammari --- Mayada S. Al-Niami --- Zainab M. Hasan
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 2 Pages: 161-168
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Aphaeresis is a term that means to separate or to take away. The basic idea of aphaeresis is efficient removal of a circulating cellular blood component, either cells (Cytopheresis) or plasma solute (plasmapheresis, plasma exchange).Thus, the treatment goal of aphaeresis is to remove the circulating cell or substance directly responsible for the disease process. Acceleration and development of aphaeresis applications had taken place with the arrival of automated cell separators in 1970s that ensure selectively removal of one or more of blood components from the blood and return the remainder to the individual. Plasmapheresis is separation of plasma from blood cells which are returned to the body. It is accompanied by multiple changes in haemostatic system. As many coagulation factors are removed during procedure, changes in selective parameters: Prothrombin Time (PT), Partial Thromboplastin Time (PTT), Thrombin Time (TT), Fibrinogen (FNG) & platelets count are found when the replacement fluids devoid from coagulation factor are used.
Patients and Methods: This clinico-haematological study conducted during a period of six months, from February 2004 to July 2004 at the National Blood Transfusion Center (NBTC) in Baghdad & 50 patients underwent Therapeutic Plasma Exchange (TPE) for various disorders with the use of two types of automated blood cell separators (Haemonetics MCS + which represent an intermittent flow centrifugation technique – IFC & Fresenius AS. TEC 204 which represent the continuous flow centrifugation technique - CFC) in this study, but no instrument type influenced the coagulation screening tests.
Results: The changes in all selective parameter: Prothrombin Time (PT), Partial Thromboplastin Time (PTT), Thrombin Time (TT), Fibrinogen (FNG), Platelets count, Haemoglobin (Hb) and Packed Cell Volume (PCV) were significant after Therapeutic Plasma Exchange (TPE). There was no significant difference in changes in crystalloid group from that in Fresh Frozen Plasma group. In crystalloid group, significant correlation was observed between Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Thrombin Time (TT) & volume of Plasma Exchanged (PE) /session, while spacing between sessions and the number of sessions is significantly correlated with Thrombin Time (TT). Plasma fibrinogen concentration and platelets count were decreased in the patients included in our current study.
Conclusion: There is no significant difference in changes in haemostatic system whether crystalloid or diluted Fresh Frozen Plasma (FFP) was used as replacement fluid. Thus, crystalloid, solution devoid of coagulation material can be used as a replacement fluid in the Therapeutic Plasma Exchange (TPE) if the volume of Plasma Exchanged (PE) is small. These results are of vital importance from the practical & public health point of view in minimizing the usage of blood components((i.e. Fresh Frozen Plasma (FFP) which is a potential source of Transfusion Transmissible Infections (TTIs) worldwide)) & replaced by crystalloid solution as a safer replacement fluid substitute in Therapeutic Plasma Exchange (TPE) process.


Article
Bacterial Contamination of Imported Bulls Frozen Semen
التلوث البكتيري في قصبات السائل المنوي المستورد للثيران

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Abstract

One hundred frozen semen straws (0.5 ml), have been taken from imported bulls, the frozen semen examination as well as evaluation in addition to bacteriological evaluation whether counting, isolation and antibiotic sensitivity, the results in current study showed all bacterial isolates were identified by using different biochemical tests and API–20 E system that used later to confirm identification, all isolated bacteria found in imported frozen semen were gram negative.45 bacterial isolates in imported frozen semen distributed in 42 isolates Stenotrophomonas maltophilia and 3 isolates Pseudomonas aeroginosa, antibiotic susceptibility test for isolated bacteria by using 14 single antibiotic discks of commonly used drugs, most isolated Steno. maltophilia was susceptible to Ceftazidime, Ciprofloxacin, Cefotaxime, Gentamicin, Amikacin, Vancomycin, Erythromycin, Chloramphenicol and Tetracyclin. The results of current study showed individual movement 61.3±2.00, viability of sperm 76.2±2.06, percentage of dead sperms 23.8±2.06, and abnormal sperms 13.54±0.67.

أخذت 100 قصبه من السائل المنوي المجمد المستورد 5. 0مل, أجريت فحوصات تقييم السائل المنوي واختبارات العد والعزل الجرثومي على العينات واختبار فحص الحساسية للمضادات الحيوية المختلفة. كل الجراثيم التي عزلت شخصت, وأجريت الفحوصات الكيموحيوية التقليدية والتشخيص بنظام API-20E لتشخيص الأنواع الجرثومية المعزولة. كانت جميع الجراثيم المعزولة من عينات السائل المنوي المجمد المستورد سالبه لصبغة كرام, خمسة وأربعون عزلة جرثومية عزلت من السائل المنوي المستورد موزعه في 42 عزله منStenotrophomonas maltophilia و3 عزلات من Pseudomonas aeruginosa. اختبرت الحساسية للجراثيم المعزولة وتم استخدام 14 نوع من الأقراص المفردة للمضادات الحيوية الأكثر استخداما, أغلب عزلات Steno. maltophilia كانت حساسة للـ Ceftazidime, Ciprofloxacin, Cefotaxime, Gentamicin, Amikacin, Vancomycin, Erythromycin, Chloramphenicol وTetracyclin, من ناحية أخرى كانت جميع الجراثيم المعزولة مقاومه بنسبة 100% لكل من الـ penicillin StreptomycinوAmoxicillin. بينت نتائج الدراسة الحالية للعينات المستوردة ان الحركة الفردية 61.3±2.00 وحيوية الحيامن 76.2±2.06 والنسبة المئوية للحيامن الميتة 23.8±2.06 أما النسبة المئوية للحيامن المشوهة فكانت13.54±0.67.


Article
Frozen Shoulder in Type 2 Diabetes Mellitus

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Abstract

Background: Frozen shoulder affects 2-5% of the general population, and around 10-30% of diabetic patients. It affect mainly the non-dominant shoulder, and has more incidence in patients with poor glycemic control.Objective: To detect the incidence of frozen shoulder in type 2 diabetic patients attending the Specialized Center for Endocrinology and Diabetes in Baghdad.Patients and methods: One hundred patients with frozen shoulder were included in the study from a total number of 580 type 2 diabetics over a period of six months. 70 patients were females and 30 patient were males. All were investigated for fasting blood glucose and HbA1c.Results: The non-dominant shoulder was involved in 60 patients (60%), the dominant shoulder in 35 patients (35%) and bilateral involvement in 5 patients (5%). 60 patients (60%) had poor glycemic control (HbA1c > 8%).Conclusion Diabetes mellitus is one of the predisposing factors for the development of frozen shoulder which affect 17.2% of type 2 diabetic patients in our study, most of them were overweight- obese and had poor glycemic control.


Article
Evaluation Some Physicochemical and Microbial Characteristics of Frozen Imported Turkey Carcasses
دراسة بعض الصفات الفيزوكيميائية والميكروبية لذبائح الدجاج الرومي المجمدة المستوردة

Author: Rashed H. Al-Dalawi رشيد حسن ألدلوي
Journal: ANBAR JOURNAL OF AGRICULTURAL SCIENCES مجلة الأنبار للعلوم الزراعية ISSN: PISSN: 19927479 / EISSN: 26176211 Year: 2011 Volume: 9 Issue: 3 Pages: 165-176
Publisher: University of Anbar جامعة الانبار

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Abstract

This study was carried out to evaluate the shelf life of frozen imported turkey carcasses, chemical and microbial characteristics. A total of 78 carcasses (39 small carcasses and 39 large carcasses) of Sadia trade marks were collected from retail storages in different regions of Baghdad and Kirkuk city during the period from 4 / 1 / 2010 to 15 / 7 / 2010. The data obtained revealed that no significant differences were appeared between large and small carcasses in thawing loss percentages, values of TBA (Thiobarbitoric Acid) and pH which were 4.4 and 4.6 %, 0.65 and 0.70 mg malonaldehyde / kg meat, 5.7 and 5.8 respectively. Small carcasses had significant (P<0.05) lower FFA (Free Fatty Acids) and PV (Peroxide Value ) test values which were 12.1 mg N/ 100gm meat, 0.50 mg/ 100gm meat and 1.22 meq/ kg meat. Where as large turkey carcasses predominant small carcasses significant (P<0.05) in total pigment, TVN, FFA and PV test values which were 129 ppm, 13.2 mg N/ 100gm meat, 0.65 mg/ 100gm meat and 1.34 meq/ kg meat. Statistical analysis showed that no significant differences were appeared between large and small carcasses in chemical composition of breast and legs meat except lipid which was significantly (P<0.05) high in large turkey carcasses in the same time moisture was low. Microbial tests appeared no significant differences in Psychotrophic bacterial count, coliform bacterial count and fungi count. The results indicated that all test values were in the allowance limits for Iraq specification of frozen chicken carcasses. The routine checking and evaluation for this meat is recommended to keep a good nutritional value for consumer.

أجري البحث لدراسة مدى صلاحية ذبائح الدجاج الرومي المجمدة المستوردة من خلال تقييم بعض الصفات النوعية والكيميائية والميكروبية . تم جمع 78 ذبيحة دجاج رومي (39 ذبيحة صغيرة الحجم و39 ذبيحة كبيرة الحجم) نوع ساديا خلال المدة من 4 / 1 / 2010 ولغاية 15 / 7 / 2010 من مناطق مختلفة من مدينتي بغداد وكركوك . وقد بينت النتائج عدم وجود فروق بين ذبائح الدجاج الرومي الصغيرة والكبيرة في نسب الفقد أثناء الإذابة وقيم حامض الثايوباربيتوريك والأس الهيدروجيني حيث بلغت معدلات القيم 4, 4 و 4,6 % ، 0,65 و 0,70 ملغم مالونالديهايد / كغم لحم و 5,7 و 5,8 على التوالي. لوحظ حصول انخفاضا معنويا (P<0.05) في قيم النتروجين الكلي المتطاير والاحماض الدهنية الحرة وقيمة البيروكسيد في لحم ذبائح الرومي الصغيرة إذ بلغت 12,1 ملغم نتروجين / 100 غم لحم و 0,50 ملغم مالونالديهايد / كغم لحم و 1.22 ملي مكافيء / كغم لحم على التوالي، بينما سجلت ذبائح الكبيرة ارتفاعا معنويا (P<0.05) في قيم النتروجين الكلي المتطاير والأحماض الدهنية الحرة وقيمة البيروكسيد حيث بلغت قيمها 13,2 ملغم نتروجين / 100 غم لحم ، 0,65 ملغم / غم ، 1,34 ملي مكافئ / كغم على التوالي. بين التحليل الإحصائي عدم وجود فروق معنوية بين ذبائح الدجاج الرومي الكبيرة والصغيرة في التحليل الكيميائي للحم الصدر والأفخاذ عدا نسبة الدهون اذ كانت مرتفعة معنويا (P<0.05) في الذبائح الكبيرة رافقها انخفاض في نسبة الرطوبة . وبينت نتائج الفحوص الميكروبية وجود تباين في أعداد البكتريا المحبة للبرودة وأعداد بكتريا القولون الكلية وأعداد الفطريات على سطح الذبائح إلا إن الفروق لم تكن معنوية إحصائيا ، وقد دلت الدراسة الحالية إن قيم الفحوص النوعية والكيميائية والميكروبية قد جاءت ضمن الحدود المسموح بها للمواصفة القياسية العراقية لذبائح الدجاج المجمدة. ونوصي بأن تخضع هذه اللحوم إلى تقييم دوري لتوفير لحوم جيدة القيمة الغذائية وصالحة للاستهلاك.


Article
Assessment of microbial effort of some types of frozen poultry in markets beyond local city of AL- hilla / Babylon governorate .
تقييم الجهد الميكروبي لبعض انواع الدجاج المجمد في الاسواق المحلية لمدينة الحلة / محافظة بابل

Authors: Hawraa wahab Azize حوراء وهاب عزيز --- tsahel Hamid AL – Dulaimi تساهيل حامد الدليمي --- Hawraa jawad kadhim حوراء جواد كاظم --- Israa Adnan Ibraheam اسراء عدنان ابراهيم
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2016 Issue: العدد الخاص بالمؤتمر العلمي الدولي الثاني لعلوم الحياة / كلية التربية للبنات / جامعة الكوفة / 2016 Pages: 45-49
Publisher: University of Kufa جامعة الكوفة

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Abstract

The frozen foods such as frozen chicken which is one of the most food consumed at the present time as consumption ratio could reach 32% at the same time represents the center of the chicken diet suitable for the growth of many bacteria and in turn leads to increased health risks for humans.The study included 70 frozen chicken sample from the origins of different (Turkish chicken 18 samples, Iranian chicken 18 samples, Saudi chicken 17 sample, chicken local sponsor 17 sample) found that the highest contamination rate was in Turkish chicken samples (21.4%) after Saudi chicken samples (20% ) then the sponsor (15.7%) and the lowest rate was in Iranian chicken samples (8.6%).

تعد الاغذية المجمدة ومنها الدجاج المجمد الذي يعد من اكثر الاغذية استهلاكا في الوقت الحاضر اذ قد تصل نسبة الاستهلاك الى 32%وفي الوقت ذاته يمثل الدجاج وسط غذائي ملائم لنمو العديد من البكتريا وبدوره يؤدي الى زيادة المخاطر الصحية للبشر.شملت الدراسة70 عينة دجاج مجمد من مناشئ مختلفة (دجاج تركي 18 عينة ,دجاج ايراني 18 عينة , دجاج سعودي 17 عينة ,دجاج الكفيل المحلي 17عينة ) وجد ان اعلى نسبة تلوث كانت في عينات الدجاج التركي (21.4 %) بعدها عينات الدجاج السعودي (20%) ثم الكفيل (15.7 %) واقل نسبة كانت في عينات الدجاج الايراني (8.6 %).


Article
EVALUATION OF MEMBRANE INTEGRITY OF BULL FROZENTHAWED SPERM USING WATER AND HYPO OSMOTIC SWELLING TEST

Authors: Godratollah Mohammadi, --- Hamed Mahdion
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2017 Volume: 16 Issue: 2 Pages: 131-143
Publisher: Basrah University جامعة البصرة

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Abstract

Assessment of the sperm membrane functional status appears to be a significant markerfor the fertilizing capacity of spermatozoa. The hypo osmotic swelling test (HOST) is one ofthe best methods to evaluate sperm membrane integrity. In the current study, we used DWand hypo osmotic solutions of 50 and 100mOsm/l of dextrose/NaCl, NaCl, sucrose andfructose. Based on the results, Among the dextrose/NaCl, NaCl, sucrose, and fructosesolutions and DW, Maximum numbers of swollen of bull frozen-thawed spermatozoa wereobserved with DW and dextrose/NaCl solution at 50 mOsm with average response by61.20±8.677 and 47.90±10.181 respectively. The HOST response at 3 and 60 min for all ofsolutions were positively correlated to each other and there was no significant differencebetween the responses to the HOST at 3 and 60 min after incubation in all of solutions. Thesignificant correlation was observed between motility and dextrose/NaCl at 50 mOsm,sucrose 50 and 100 mOsm, NaCl 50 mOsm and DW. The high relationship was betweenmotility and DW and dextrose/NaCl at 50 mosm. There was no significant correlationbetween DW and all of hypoosmotic solutions with staining of the spermatozoa byeosin/nigrosin. In conclusion, the water test can be efficiently used for the evaluation of thefunctional integrity of the plasma membrane of bull frozen-thawed spermatozoa. Thehypoosmular solution of dextrose/NaCl at 50 mOsm is a good medium to evaluate bull frozenspermatozoa. The used of HOST and motility are better tests to evaluate bull frozen thawedsperm than eosin-nigrosin. The short HOST procedure (3 min) is suitable method forevaluating of membrane integrity of bull frozen/thawed spermatozoa


Article
4-EVALUATION OF MEMBRANE INTEGRITY OF BULL FROZEN-THAWED SPERM USING WATER AND HYPO OSMOTIC SWELLING TEST

Authors: Godratollah Mohammadi, --- Hamed Mahdion
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 1 Pages: 38-51
Publisher: Basrah University جامعة البصرة

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Abstract

Assessment of the sperm membrane functional status appears to be a significantmarker for the fertilizing capacity of spermatozoa. The hypo osmotic swelling test(HOST) is one of the best methods to evaluate sperm membrane integrity. In thecurrent study, we used DW and hypo osmotic solutions of 50 and 100mOsm/l ofdextrose/NaCl, NaCl, sucrose and fructose. Based on the results, Among thedextrose/NaCl, NaCl, sucrose, and fructose solutions and DW, Maximum numbers ofswollen of bull frozen-thawed spermatozoa were observed with DW anddextrose/NaCl solution at 50 mOsm with average response by 61.20±8.677 and47.90±10.181 respectively. The HOST response at 3 and 60 min for all of solutionswere positively correlated to each other and there was no significant differencebetween the responses to the HOST at 3 and 60 min after incubation in all ofsolutions. The significant correlation was observed between motility anddextrose/NaCl at 50 mOsm, sucrose 50 and 100 mOsm, NaCl 50 mOsm and DW. Thehigh relationship was between motility and DW and dextrose/NaCl at 50 mosm. Therewas no significant correlation between DW and all of hypoosmotic solutions withstaining of the spermatozoa by eosin/nigrosin. In conclusion, the water test can beefficiently used for the evaluation of the functional integrity of the plasma membraneof bull frozen-thawed spermatozoa. The hypoosmular solution of dextrose/NaCl at 50mOsm is a good medium to evaluate bull frozen spermatozoa. The used of HOST andmotility are better tests to evaluate bull frozen thawed sperm than eosin-nigrosin. Theshort HOST procedure (3 min) is suitable method for evaluating of membraneintegrity of bull frozen/thawed spermatozoa.


Article
Although Late; but the First, an Iraqi Success in Human Embryo Cryopreservation Using Vitrification and the Factors Affecting the Pregnancy Rate: Cross-Sectional Study

Authors: Thuraya H. Abdulla --- Ula M. Al-Kawaz --- Ali I. Rahim
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 1 Pages: 10-21
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: it is important to study a history of the science to appreciate thepast as a motive for hard work in the present for better future. In the other hand,the factors affecting pregnancy rate of frozen embryos are yet to be clarified atdifferent embryonic developmental stages.Objectives: to record a brief history of embryo cryopreservation in Iraq and tostudy many clinical and embryological factors that might affect the pregnancyrate.Subjects, materials and Method: a cross-sectional study of many clinical and embryologicalvariable, where120 frozen-thawed embryo transfer cycles enrolled inthe study at the period from January 2017 till July 2018. The variables were statisticallyanalyzed first by one factor analysis comparing between pregnant andnon-pregnant cases; then by multivariate logistic regression analysis to illustratemain factors without a possible confounder effect.Results: In one factor analysis, the following variables showed a significantlyhigh effect on pregnancy rate (P value <0.001) which are women’s age, women’sweight, number of retrieved oocytes, mature oocytes and good quality embryos,developmental stage, E2 level, and endometrial thickness. While in multivariatelogistic regression analysis the women’s weight, the number of retrieved oocytesand good quality embryos were the main factors.Conclusions: it is judiciously to consider these factors while managing infertilecouples with embryo cryopreservation programs especially the modifiable factors.

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