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Article
Gene Expression Bcl-2 Gene in Cancer of Brest in Iraqi population

Author: H. M. Al-Khafaji
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2017 Volume: 35 Issue: 2 Part (B) Scientific Pages: 119-125
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

The breast cancer is dangerous disease in the world . Molecular methods are important and necessary to diagnose breast cancer. Many of the genes with expression change like Bcl-2 gene is specifically, coding an anti-apoptotic protein and and therefore classified as an oncogene. Determine the damage of Bcl- 2 gene as a cause of some types of cancer, such as breast cancer, leukemia, prostate cancer and lung cancer .In this study, we examined Bcl-2 expression levels in (malignant, benign and healthy) tissues of the breast .They were fifty Laboratory samples (18 cancer tumor , 12 benign and 20 marginal (non-cancer) breast tissue that diagnosed based on their information were obtained from their files and records in all patients in this study , to extract the DNA and measure the level of expression of gene under study by molecular technique of ( r –t PCR ). Expression of gene under study is higher levels in malignant group and the fold of expression was 10.00 time higher than the control group and also in the benign group the fold of expression was 2.18 that higher than the control group.The results showed the expressed gene Bcl-2 is significantly higher in the third grade of breast tumor samples with Ct (22.14) of the first grade with CT (25.63) and the second with CT (24.07). According to the results of the study that the use of molecular methods in measuring the expression of Bcl-2 gene may help to diagnose the disease and may be considered that the Bcl2 gene is molecular tool for the early detection of breast cancer


Article
Chromosomal Aberrations and Gene Expression Study in Breast Cancer Patients Undergoing Radiotherapy

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Abstract

The present study aims to use the chromosomal aberrations and gene expression analysis as biomarkers for detection of the effects of ionizing radiation exposure in breast cancer patients (BC) undergoing radiotherapy about 20-30 Gy locally gamma cells, which may effect DNA of cancer patients. This study was carried out on thirty. Iraqi women patients with breast cancer patients during radiotherapy treatment at Al-Amel National Hospital for cancer Management in Baghdad during time 2-13 years , non-smokers and non- alcoholic, aged (30 - 59 year), with stage (grade) I - III, as well as thirty apparently healthy individuals females collected randomly from population living Baghdad , aged ranged (30 - 59 year) which are non-smokers non- alcoholic as control group. Using two molecular genetic end-points parameters were studied to determine genotoxic effects of radiotherapy in peripheral blood lymphocytes of some Iraqi breast cancer patients and compared with control groups. Investigations were carried out by using the chromosomal aberrations (CA) and gene expression were performed on peripheral blood lymphocytes for breast cancer patients and control groups.The present study showed significant increase (p<0.01) in the unstable chromosomal aberration types (CA) fragment, ring and dicentric chromosomes for the breast cancer patients during radiotherapy as compared with the control group. Also, This study including twenty. Iraqi women patients with breast cancer after radiotherapy treatment about 20-30 Gy locally exposure to gamma rays , aged ( 35 - 55 years), as well as twenty female blood samples, aged (35 - 55 years) which are non- smokers or alcoholic as control group. Total RNA was isolated from blood for BC patients and control groups.The RNA concentration was determined spectrophotometrically by measuring their absorbance that dependent on the ratio A260/A280 of the wavelength, which leads to the determination of RNA purity, which ranged from 1.79-2.1 in two groups. Complementary DNA was used in amplification of genes used in the present study, three types of specialized primer genes were selected for the genes CDKN1A, BRCA1 and BRCA2 which have a relation with ionizing radiation in addition to the primers for internal control (β-actin) genes. Gene expression analysis revealed statistically significant (∆∆Ct comparative Ct method) transcriptional changes in two genes CDKN1Aand BRCA2 up-regulated while BRCA1 gene down-regulated. In conclusion, the results indicated that there is a possibility of using the changes at the level of CA as useful biomarkers for the detection of the effect of radiotherapy in peripheral blood lymphocytes for BC cancer patients. several genes involved in cell cycle regulation and DNA repair were found to be significantly induced by radiation treatment.


Article
Epstein Barr Virus and P53 Gene Expression Correlation with Gastric Adenocarcinoma Patients in Baghdad City

Author: Noor AL-Huda Ali A. H. Saeed
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 1 Pages: 73-82
Publisher: Baghdad University جامعة بغداد

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Abstract

Gastric carcinoma is the most prevalent cancer related deaths worldwide today . The current study was carried out to shed the light on Epstein Barr virus, Tumor suppressor gene p53 associated gastric cancer of some Iraqi patients. According to insitu hybridization technique , this study was determined EBV and p53 gene correlates with some aspects of gastric cancer patients like age, gender , histological grade and stage of the tumor . Fourty six biopsies were obtained from patients with adenocarcinoma who had undergone gastrectomy. The tissue sections were collected during the period between September 2010 until June 2013. Epstein Barr virus was detected insitu hybridization in 18 patients (39%) out of 46. Whereas, the positive results of relation between p53 oncogene and gastric adenocarcinoma patients were detected in 30 (65.2%) out of 46. Out of 46 patients , this study included 32 male and 14 female with mean age 54 ranged between 30-72 years. The histological types included 18 well, 4 moderate and 24 poor differentiated respectively . Most of cases 32 (69.5%) falling in stage I-II and the remaining 14 were in the stage III and IV. The positive results revealed that EBV correlate in highly significant association with each of age, gender, grade and stage of the tumor , also expression of tumor suppressor gene p53 was correlation with all patients aspects in highly significant association.


Article
Effect Of Castration Methods On Gene Expression Of Androgen Receptor Gene In Skeletal Muscles Of Awassi Sheep
تأثير طريقة الاخصاء على التعبير الجيني لجين مستقبلات الاندروجين في العضلات الهيكلية لأغنام العواسي

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Abstract

The effect of castration on skeletal muscle development in awassi sheep were studied at the gene expressed for androgen receptor. The result showed that live weigh for epididymectomy rams was heavier than control and orchidectomy rams, respectively. There was significant difference (p < .05). By using the (2^ΔCT) with reference gene method, the findings documented that there was higher up-regulated in mRNA expression for androgen receptor in splenius muscles cells for epididymectomy rams compare with interact rams. In contrast, there was down-regulated for mRNA expression for androgen receptor cells for orchidectomy rams. In conclusion the continuous producing of testes hormones lead to growth of skeletal muscles of wether's significant increase the growth of skeletal muscles of wethers by epididymectomy companied with other rams.

تم دراسة تأثير نوع الأخصاء على تطور العضلات الهيكلية في اغنام العواسي من خلال تعيين التعبير الجيني لجين مستقبلات الاندروجين, حيث أظهرت النتائج بان الوزن الحي للاكباش المخصية بطريقة ازالة جزء من البربخ كان وزنها اعلى من الاكباش في مجموعة السيطرة والاكباش المخصية بطريقة ازالة الخصية حيث انه كات هناك فرق معنوي (p < .05)كما واثبتت الدراسة بان هناك مستوى عالي للتعبير الجيني للحامض النووي الرايبوزي الناقل لمستقبلات الاندروجين في خلايا العضلة العنقية للاكباش المخصية بطريقة ازالة جزء من البربخ مقارنة مع الاكباش السليمة على العكس من ذلك فأن النتائج اظهرت أن التعبير الجيني لجين مستقبلات الاندروجين كان واطئ لنفس الخلايا في الاكباش المخصية بطريقة ازالة الخصية, من هنا نستنتج بأن استمرارية الخصية بانتاج الهرمونات يؤدي الى زيادة معنوية في نمو العضلات الهيكلية للاكباش المخصية بطريقة ازالة جزء من البربخ مقارنة مع بقية الاكباش الاخرى.


Article
Bioinformatics: Effect of Cytomegalovirus Infection on Human Immune Gene Expression
معلوماتية حيوية: تأثير إصابة فيروس المتضخم الخلوي على التعبير الجيني في الإنسان

Author: Abdulrhem Th. Al-Ghazal عبد الرحيم ذنون الغزال
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2018 Volume: 27 Issue: 5E-Microbiology Pages: 8-18
Publisher: Mosul University جامعة الموصل

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Abstract

Cytomegaloviruses (CMV) cause common infections in humans with a prevalence of over 70% in adults, reaching 90% in poorer communities and developing countries. In immune-compromised and transplant recipients, CMV causes serious complications including pneumonia and ulcerative colitis. Studying the effect of CMV on human immune-inflammatory genes in immune cellular responses is critically important to explore the molecular mechanisms of viral-host interaction. The aim of this study is to explore changes in the mRNA transcripts of a panel of 84 human inflammatory genes. Ninety blood samples were collected from healthy persons used as control samples and compared with acute infection with CMV sample from bioinformatics data (previous researches). Real time-PCR array were performed to determine mRNA levels expression levels of 84 different cytokines and chemokines in control`s leukocytes concentrate. CMV infection caused upregulation (between 44.99 and 0.92 fold change) in the expression of 13 human immune-inflammatory genes (IL-1 alpha, IL10R Alpha, CXCL10, IL10, CX3CR1, CCL2, CCR5, CCR7, CXCL1, IL-1β, TNF, IFN-alpha 2, CCL5). Gene ontology analysis revealed that CMV causes high impact on four key pathways in infected cells.

يسبب الفيروس المتضخم الخلوي إصابات عامة ومتعددة للإنسان وبنسبة انتشار تصل الى 70% في البالغين وتصل الى 90% في مجتمعات الدول النامية. في الأشخاص ضعيفي المناعة والمجرى لهم عمليات نقل اعضاء، يسبب الفيروس مضاعفات خطيرة مثل ذات الرئة وقرحة القولون. ان دراسة تأثير إصابة فيروس المتضخم الخلوي على مستوى التعبير الجيني المناعي الالتهابي داخل خلايا الإنسان تعتبر مهمة لمعرفة طبيعة الميكانيكية الجزيئية الحاصلة بين الفيروس والخلية المضيف. الهدف من هذا البحث هو دراسة التغيرات الحاصلة في نسخ الحامض النووي الرايبوزي (التعبير الجيني) ل 84 جين مناعي التهابي في الإنسان عند الإصابة بهذا الفيروس. تم جمع تسعين عينة دم من أشخاص أصحاء للمقارنة مع أشخاص مصابون بالفيروس في دراسات سابقة. تم استخدام تقنية تفاعل البلمرة المتسلسل الاني لتحديد مستوى التعبير الجيني (نسخ الحامض النووي الرايبوزي) للجينات المناعية في كريات الدم البيضاء. أظهرت الدراسة زيادة في تضاعف التعبير الجيني ل 13 جين مناعي وبمدى تضاعفي يتراوح بين 0.92 و 44.99 ضعف (IL-1 alpha, IL10R Alpha, CXCL10, IL10, CX3CR1, CCL2, CCR5, CCR7, CXCL1, IL-1β, TNF, IFN-alpha 2, CCL5).. تحليلات التنبؤ الجيني اظهرت تأثيرا كبيرا لفيروس المتضخم الخلوي على اربعة مسارات حيوية مناعية في الخلايا المصابة.


Article
Assessment of the Role of Gender in the Expression of UGT1A1 Gene in Hyperbilirubinemic Neonates

Author: Hussein Hameed Hassan Mudhaji حسين حميد حسن
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2014 Volume: 7 Issue: 1 Pages: 1780-1785
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Neonatal jaundice (NJ) is a significant disease among neonates in Najaf province. It manifests 19% of the total deliveries and 50% of the in-patients, and affects about 60% of term and 80% of preterm neonates during the first week of life. UGT1A1 is a cause of concern in NJ, because it is the most important underlying cause of unconjugated hyperbilirubinemia.Methods: A cohort of 85neonate sorted into three groups according to the TSB level. Group 1 (TSB<5mg/dl), group 2 (TSB 5-15 mg/dl) and group 3 (TSB <15mg/l). UGT1A1expression and TSB level was assessed for both males and females in each group.Results & Discussion: Results showed high inverse correlation between UGT1A1expression and the ratio of males (r= -0.99, P<0.01), while females ratio ex-pressed high direct correlation with UGT1A1 expression level with correlation coeffi-cient of (r=0.99, P<0.01).This might due to physiological maturity differences of liver between both sexes, and the plasma growth hormone (GH) pulses frequencies (released by the pituitary gland) which affects the expression of liver enzymes. It's found that GH pulses are higher in females than in males. Conclusion: concluding that mean UGT1A1 expression level in females is more than that in the males during neonatal period therefore females are less vulnerable to NJ than males.Aim: The aim of this study is to qualify the UGT1A1 gene expression in both males and females therefore determining health and health care requirements.


Article
Increased Transforming Growth Factor‑β and Interleukin‑17 Transcripts in Peripheral Blood of Breast Cancer Patients with Different Clinical Stages

Author: Hadeel Saleem Touma, Wafaa Sadoon Shani
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 145-149
Publisher: Babylon University جامعة بابل

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Abstract

Background: Currently, cancer as major problem around the world threatens human health and has a high incidence in developing countries.Many reports have showed that there is a dysfunction in the immune system of cancer patients. Interleukin‑17 (IL‑17) and transforming growthfactor‑β (TGF‑β) are increased in the generated through cancers, and IL‑17 is considered as a pro‑inflammatory cytokine; IL‑17 is an angiogenicfactor and promotes tumor growth. Materials and Methods: In this study TGF‑β and IL‑17, mRNA gene expression has been measuredin peripheral blood of 35 breast cancer patients and 35 normal age‑matched women using quantitative real‑time polymerase chain reactionmethod with master mix reaction containing SYBER Green. β‑actin gene was used as housekeeping gene. Results: Our data demonstrateda significant upregulation of TGF‑β and IL‑17 gene expression (P ˂ 0.05) in patient’s peripheral blood compared to normal healthy control.Levels of genes expression were higher in the breast cancer group compared to the control group. Conclusion: TGF‑β and IL‑17 expressioncould be used as a diagnostic marker for detecting breast cancer.


Article
Molecular and Nanotechnical Study for Antibiofilm Formation and CsuE Gene Expression Activities of Synthesized Iron Oxide Nanoparticles Against Multidrug-Resistant Acinetobacter baumannii Isolates

Author: Haider Turky AL-Mousawi1 , Mohammed I. AL-Taee2 , Maarib N. Rasheed2 , Qabas Nima AL-Hajjar3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 201-2015
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to investigate the influence of synthesis of Iron Oxide Nanoparticles (Fe3O4NPs) by co-precipitation method on biofilm formation and CsuE gene expression in multidrug resistance Acinetobacter baumannii (MDRA.b) which represents one of the important causing agents of nosocomial infection. A. baumannii isolates used in current study and isolates from different clinical sources (wounds, burns, urine, sputum, blood and throat) that able to produce strong biofilm. Synthesis and characterisation the physical-chemical nature of Fe3O4 NPs was carried out using UV-visible spectrophotometer, fourier transform infrared (FT-IR), X-ray diffraction (XRD), Atomic force microscope (AFM) and scanning electron microscope (SEM). Results show that they were very fine crystalline sizes reaching to 11±1 nm by XRD ,with a mostly spherical in shape and average of nanoparticles size between (40- 47) nm by SEM and AFM, respectively. Minimum inhibitory concentration (MIC) and sub-MIC test of Fe3O4 NPs at concentrations (15.75 to 2000) μg/ml against A.baumannii isolates had been determined using tube broth method measured by optical densities values at 630 nm. The results of the experiment of the best sub-MIC with concentration of Fe3O4 NPs 125μg/ml showed a significant difference at p˃0.05 the anti-biofilms inhibitory on polystyrene surface of microtiter plates for the bacteria isolate under study. Biofilm formation CsuE gene expression was investigated by using RT-qPCR technique with reference 16SrRNA gene before and after treatment with Fe3O4 NPs. The results showed a significant difference at p˃0.05 in Cycle threshold (Ct) values for CsuE gene expression of the isolates. The study concludes that the synthesized magnetic Fe3O4 NPs with 125µg/ml gave excellent antibiofilm activities and inhibitory efficiency against A.baumannii isolates and the potentiate to down regulate of gene expression fold for biofilm formation encoded by CsuE gene of A.baumannii leading as a result to have low biofilm production.


Article
GHRELIN GENE POLYMORPHISMS AND EXPRESSION BOND WITH SOME GROWTH AND CARCASS TRAITS OF ROSS 308 BROILER CHICKENS
طرز جين الكرلين و مستويات تعبيره وعلاقتها مع بعض صفات النمو والذبيحه في فروج اللحم روز 308

Author: Joody & et al جودي وآخرون
Journal: Iraqi Journal of Agricultural Science مجلة العلوم الزراعية العراقية ISSN: 00750530/24100862 Year: 2019 Volume: 50 Issue: 4 Pages: 1056-1063
Publisher: Baghdad University جامعة بغداد

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Abstract

This experiment was conducted to study the association of Ghrelin gene polymorphisms and expression levels with some productive and carcass traits of broiler chickens. Two hundred broiler chicks, one day old Ross308, were wing-tagged and reared under conventional conditions. Blood samples were collected individually of all birds to study of ghrelin gene polymorphisms by PCR-RFLP technique. Proventriculus was collected at 35 days of age from thirty birds of each groups sorted according to high, moderate and low growth rate to measure ghrelin gene expression by real-time RT-PCR. Result revealed that final body weight and weight gain were not significantly influenced by ghrelin gene , whereas body weight and weight gain of males and females with high ghrelin gene expression were significantly (p<0.01) higher than those of moderate and low ghrelin gene expression at 21 and 35 days of age. Ghrelin gene polymorphisms had no significant (p>0.05) influence on carcass traits and edible organ weights, while significant (P < 0.01) differences were existed among high, moderate and low ghrelin gene expression groups in carcass weight, breast, wing, neck and back relative weights. It is concluded that PCR- RFLP technique revealed no significant effect for ghrelin gene polymorphisms on productive and carcass traits, whereas ghrelin gene expression had significant effect on productive traits.

أجريت هذه الدراسة لبحث العلاقة بين الطرز الوراثية لجين الكرلين و مستويات تعبيره مع بعض الصفات الإنتاجية لفروج اللحم. استخدم مائتان فرخ بعمر يوم واحد تم وزنها و ترقيمها بالجناح و ربيت تحت الظروف المثالية. جمعت عينات الدم من الأفراخ جميعها لدراسة الطرز الوراثية لجين الكرلين باستخدام تقنية تباين أطوال قطع التقييد RFLP المبنية على أساس التفاعل التضاعفي لسلسلة الدنا PCR . جمعت المعدة الغدية Proventriculus عند عمر 35 يوم من ثلاثون طير من كل مجموعة من الطيور التي تتميز بمعدلات النمو العالية, المتوسطة و الواطئة لتقدير التعبير الجيني للكرلين باستخدام تقنية real-time RT-PCR. أظهرت النتائج أن وزن الجسم النهائي و الزيادة الوزنية لم تتأثر معنويا بالطرز الوراثي لجين الكرلين لكنه أوزان الجسم و الزيادة الوزنية لذكور وإناث فروج اللحم التي تملك تعبير جيني عالي كانت واضحة الارتفاع (p<0.01) مقارنتا مع فروج اللحم الذي يمتلك تعبير جيني متوسط أو منخفض في اليوم 21 و 35 من العمر. عدم وجود تأثير معنوي (p>0.05) لطرز جين الكرلين في خصائص الذبيحة و الأوزان النسبية للأعضاء الصالحة للأكل لكن التأثير واضح (P < 0.01) بين مختلف مستويات التعبير الجيني للكرلين (المستوى العالي و المتوسط و المنخفض) في الأوزان النسبية للذبيحة, الصدر, الجناح, الرقبة والظهر. تقنية PCR- RFLP لم تظهر أي تأثير واضح لطرز جين الكرلين في أداء و خصائص الذبيحة لكن التعبير الجيني للكرلين اظهر تأثير واضح في أداء فروج اللحم.


Article
The Effect of Ficus Religiosa Chloroform Extract on Suppression of Acquired Docetaxel Resistance in Prostate Cancer
تاثير المستخلص الكلوروفوم لنبات لسان العصفور على تثبيط الخلايا السرطانية المكتسبة لمقاومة دوكسيتاكسيل في سرطان البروستات

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Abstract

Most of the currently used cancer therapeutics are natural products. These agents were generally discovered based on their toxicity to cancer cells using various bioassays. Ficus religiosa (FR) plant is important medicinal plant and traditionally used to treat various diseases including mastitis, otitis media, pharyngolaryngitis, urethritis, dysmenorrhea and diabetic. Accordingly, it was aimed to investigate the cytotoxic effect of Ficus religiosa chloroform extract on Patch1 and Gli2 gene expression in Hedgehog pathway and Id1, Id2 and Id3 genes expression in inhibition differentiation pathway on prostate cancer cells, which are resistance to docetaxel (PC3-TxR) in vitro. Chloroform extract of Ficus religiosa plant leaves was performed to tested cytotoxic effect on PC3-TxR by using Sulforhod-amine-B assay and its ability to inhibition genes expression on PC3-TxR cells by using RT-PCR assay. The results showed that F. religiosa chloroform extract display high cytotoxic effect (IC50= 0.3±0.02 mg/ml), and inhibition effect of Id2 and Id3 gene expression more than, Patch1 and Gli2 gene expression on PC3-TxR cells. The present study showed anticancer effect of F. religiosa chloroform extract which target Id pathway on PC3-TxR cells.

معظم العلاجات السرطانية المستخدمة حاليا هي منتجات طبيعية. تم اكتشاف هذه الاهمية بصفة عامة على أساس سميتها على خلايا السرطانية باستخدام مختلف اختبارات بيولوجية. نبات لسان العصفور (Ficus religiosa) هو من النباتات الطبية الهامة والتي تستخدم عادة لعلاج مختلف الأمراض بما في ذلك التهاب الضرع، التهاب الأذن الوسطى، التهاب البلعوم و الحنجرة، التهاب الإحليل، وعسر الطمث والسكري. لدراسة تأثير السمي لمستخلص الكلوروفورم لاوراق نبات لسان العصفور على الخلايا السرطانية باستخدام SRB assay ودراسة تاثيره على التعبير الجيني لجينات Patch1) و(Gli2 في مسار hedgehog والتعبير الجيني لجينات (ID1، ID2 وID3) في مسار Inhibition differentiation في خلايا سرطان البروستاتا المقاوم لدوسيتاكسيل (PC3-TXR) باستخدام تقنية RT-PCR في المختبر. وأظهرت النتائج أن مستخلص الكلوروفورم لاوراق F. religiosa تأثير سمي عالي (IC50 = 0.3 ملغ / مل)، وتأثيره التثبيطي على التعبير الجيني للـ (ID1، ID2 وID3) أكثر من تاثيره التثبيطي على التعبير الجيني لل( Patch1 و(Gli2 في خلايا البروستات السرطانية PC3 -TXR. ونستنتج من ذلك ان المستخلص الكلورفورم لاوراق نبات لسان العصفور له تأثير سمي ومثبط لسرطان البروستات المقاومة لعقار الدكسيتاكسيل (PC3-TxR).

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