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Article
Helicobacter Pylori Infection in Diabetic Patients

Author: Sabeha M. Al-Bayati
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2010 Volume: 9 Issue: 1 Pages: 31-34
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background H Pylori infection could be regarded as the commonest infection worldwide, it had been suggested that H pylori infection is more frequent among diabetics. Objective This study had been designed to define any significant association between diabetes and H pylori infection, to evaluate different demographic features of patients with diabetes mellitus who have H pylori infection, assess any relation between H pylori infection and the metabolic control of diabetes mellitus and to estimate the frequency of different oesophagogastroduodenoscopy findings among diabetic population with H pylori infection. Methods This study enrolled 50 patients with diabetes mellitus and another 50 non-diabetic patients (as a control group). The patients attended Gastrointestinal unit at Al-Yarmouk Teaching Hospital Baghdad/Iraq during the period between the 1st of March2004, to the 31st of November 2004. Full history was taken and clinical examination, investigation to assess the glycemic control and esophagogastroduodenoscopy (OGD)had been done to all of them. H pylori status had been detected by positive Rapid Urease test and histopathology and/or ELIZA test for anti-H pylori IgG. Results This study revealed that 26diabetic patients (52%) were H pylori positive, while 14 non-diabetic patients (28%) were H pylori positive which is statistically significant (X2=6.0,P=0.01). patients aging 60 year old or more (24 patients, 48% of the sample) 18 of them had positive H pylori status, 20 patients out of the 26patients with positive H pylori status (76.9%) had poor glycemic control, 84.6% of those with positive H pylori status (22 out of 26 patients) had glycated hemoglobin level of 8% and greater, 61.8% of those with positive H pylori status (16 patients) were diabetic for more than 10 years. 61.5% of those with positive H pylori status had one or more of the chronic diabetic complications, 12 patients had OGD finding consistent with duodenitis, all of them were H pylori positive, OGD examination revealed 20 cases without active disease, 18 of them had negative H pylori status. Conclusions This study indicates that Helicobacter pylori infection is more common in diabetic patients. Frequency of H pylori infection is higher among elderly diabetics, those with long standing, poorly controlled and with diabetic complications.


Article
The Detection of Neutrophiles in Gastric Mucosa of Patients Suspected to be Infected with Helicobacter Pylori Using Leukostix

Authors: Nassir Enssief Mohsun --- Raji H AL-Hadithi --- Suhaila Saadallah
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 4 Pages: 480-483
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori has been detected in many populations and associated with inflammation of gastro duodenal mucosa. Colonization of the stomach by Helicobacter pylori occurs in more than half of human population worldwide. It is the principle cause of chronic active gastritis, peptic ulcer and gastric cancer.OBJCTIVE:To detect neutrophiles in homogenates biopsied gastric mucosa semiquantitatively using rapid leukocyte strip test (leukostix).METHODS:A total of 115 patients (74 males, 41 females) referred to The Gastrointestinal Tract Center and Gastroscopy Department of Baghdad Medical City and subjected to gastroscopy were included in this study during the period from November 2004 to May 2005.RESULTS:The sensitivity and specificity of leukostix at the initial examination were 95.8%, 88.23% respectively.CONCLUSION:The leukostix test, using biopsied samples of gastric mucosa was excellent for quantitative determination of neutrophils in patients infected with H pylori


Article
Systemic Humoral Anti Helicobacter pylori Immune Response in Dyspeptic Patients

Author: Batool M. Mahdi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 153-158
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori (HP) cause a continuous gastric inflammation in virtually all infected persons. It induces a vigorous systemic and mucosal humoral and cellular immune response. In spite of these responses, the vast majority of infected hosts are unable to clear the infection and it persists for decades.OBJECTIVE:Determination the humoral immune response to HP infection in dyspeptic patients.PATIENTS AND METHODS:The immunological serological tests were evaluated in 80 dyspeptic patients divided into two groups: (HP +) and (HP-). Levels of specific HP IgG antibodies were determined through a specific immunological non invasive Enzyme Linked Immuno Sorbent Assay (ELISA) test from Biohit PIC, Helsinki, Finland. Immunoglobulin levels and complement were done (IgG, IgA, IgM, C3 and C4) using single radial immune diffusion (BioMaghreb-Tunis).RESULT:About 62.5 % of dyspeptic patients had HP + infection. There was no significant differences between two groups in the levels of (IgG, IgA, IgM, C3 and C4) and most of them were within normal values.CONCLUSION:Humoral immune response had an important role in the control and limits the inflammation of gastric mucosa.


Article
The Detection of Neutrophiles in Gastric Mucosa of Patients Suspected to be Infected with Helicobacter Pylori Using Leukostix Nassir Enssief Mohsun*,

Authors: Suhaila Saadallah *** --- Raji H AL-Hadithi**,
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 4 Pages: 480-483
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Helicobacter pylori has been detected in many populations and associated with inflammation ofgastro duodenal mucosa. Colonization of the stomach by Helicobacter pylori occurs in more thanhalf of human population worldwide. It is the principle cause of chronic active gastritis, peptic ulcerand gastric cancer.OBJCTIVE:To detect neutrophiles in homogenates biopsied gastric mucosa semiquantitatively using rapidleukocyte strip test (leukostix).METHODS:A total of 115 patients (74 males, 41 females) referred to The Gastrointestinal Tract Center andGastroscopy Department of Baghdad Medical City and subjected to gastroscopy were included in thisstudy during the period from November 2004 to May 2005.RESULTS:The sensitivity and specificity of leukostix at the initial examination were 95.8%, 88.23%respectively.CONCLUSION:The leukostix test, using biopsied samples of gastric mucosa was excellent for quantitativedetermination of neutrophils in patients infected with H pylori.


Article
5- EVALUATION OF ENDOSCOPY BASED METHODS (HISTOPATHOLOGY, CYTOLOGY AND UREASE TEST) FOR THE DETECTION OF HELICOBACTER PYLORI

Authors: Sawsan S Al-Haroon --- Sarkis K Strak --- Rafid Adil Abood
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2009 Volume: 15 Issue: 1 Pages: 21-24
Publisher: Basrah University جامعة البصرة

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Abstract

This study aimed to assess the accuracy of histopathology, brush cytology, andurease test in the diagnosis of Helicobacter pylori and to evaluate the effect of testduration on the sensitivity and specificity of positive urease test for the detection of H.pylori.Fifty patients [25 patients with gastritis group A and 25 patients with duodenal ulcergroup B] selected from those attending endoscopy unit for dyspeptic symptoms, wereenrolled in the study. Four endoscopic biopsies were taken from each patient. Onebiopsy from each of antrum and body were obtained for urease test (Urease test wasread at 30 min, 1, 4 and 24 hour after biopsy insertion into the reagent), and onebiopsy from each of antrum and body were used for histopathological examination.Antral brush cytology was taken also from each patient. The patients were consideredH. pylori positive when minimum concordances of 2 out of 3 tests (Histopathology,brush cytology, and urease test) were positive.Fourteen patients were positive for H. pylori in group A, in comparison to seventeenpatients in group B. The sensitivities of the histopathological examination, brushcytology, and urease test at 24 hours in group (A) were 58%, 79%, and 93%respectively. Corresponding figures for the specificity were 100%, 91%, and 46%respectively. While in group (B) the sensitivities were 82%, 82%, and 100% and thespecificities were 100%, 100%, and 88% respectively.It is concluded that among the invasive methods, the association of the urease testwith brush cytology constituted the best choice for confirming the diagnosis of H.pylori, due to the high sensitivity of the urease test and high specificity of brushcytology.


Article
Haemagglutination (HA) test of Helicobacter pylori
اختبـار التـلازن الدموي لبكتريـا Helicobacter pylori

Authors: Saba A.S.H. Al-Sultan صبا عبد السلام حامد السلطان --- Subhi H.K.Al-Jubouri صبحي حسين خلف الجبوري
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 3ملحق ابحاث المؤتمر التنوع البايولوجي Pages: 1382-1386
Publisher: Baghdad University جامعة بغداد

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Abstract

Thirty clinical isolates of Helicobacter pylori bacteria obtained from patients attending endoscopy unit of Ibn-Sena teaching hospital in Mosul . These patients were complaining from epigastric pain , dyspepsia , acidity , vomiting , abdominal pain , flatulance , heart burn and melena . The H. pylori isolates were used for Haemagglutination assay (HA) , which involves the recognition of various glycoconjugates on the surface of red blood cells . In this study sheep red blood cells has been used in (HA) assay because the sheep erythrocytes surface resemble that of human epithelial cells .The results proved by (HA) assay, the ability of H. pylori to adherence to specific receptors on the surface of Human Epithelial Cell , which is the first step in the pathogenic process .

ثلاثين عزلة سريرية من بكتريا Helicobacter pylori حصل عليها من المرضى الخاضعين لفحص الناظور في مستشفى ابن سينا التعليمي في الموصل والذين يشكون من الألم في الشرسوف وعسر الهضم والحموضة والقيء والم في البطن وانتفاخ البطن وحرقة الفؤاد وخروج اسود اللون. واستخدمت عزلات بكتريا H.pylori لإجراء اختبار التلازن الدموي والذي يتضمن تحديد التراكيب السكرية المقترنة على أسطح كريات الدم الحمراء . وفي هذه الدراسة تم استخدام كريات الدم الحمـراء للأغنام لكونها تشبه في مكوناتها السطحية للخلايا الطلائية للإنسان .وبرهنت نتائج هذه الدراسة باستخدام اختبار التلازن الدموي من قابلية بكتريا H.pylori على الالتصاق على مستقبلات متخصصة على أسطح كريات الدم الحمراء للأغنام وهذا يعني وبعبارة اخرى قابليتها على الالتصاق على أسطح الخلايا الطلائية للإنسان والتي تعد الخطوة الأولى في إمراضيه هذه البكتريا .


Article
HELICOBACTER PYLORI INFECTION AMONG PATIENTS WITH DIABETES MELLITUS

Authors: Ali K Jumaa --- Sarkis K Strak
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2015 Volume: 21 Issue: 1 Pages: 12-17
Publisher: Basrah University جامعة البصرة

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Abstract

Helicobacter pylori (H. pylori) is a gram-negative bacterium that specifically colonizes the gastric epithelium causing many complications. The link between H. pylori infection and diabetes mellitus (DM) remains controversial. This study was conducted to determine the frequency of H. pylori infection among a group of subjects with DM and to compare it with that of an age and sex-matched group of non-diabetic subjects. This case control study was conducted in Al-Sadr Teaching Hospital, Basrah, Southern Iraq from January 2013 to September 2014 on 200 subjects aged ≥ 40 years, 100 diabetic and 100 non-diabetic subjects. All subjects were required to submit fresh stool samples which were tested for evidence of H.pylori infection by stool antigen positivity. Helicobacter pylori infection was detected in 43% of diabetic group and 25% of controls, which was found to be statistically significant ( p value=0.007). In conclusion, the present study suggests that diabetic subjects are at more risk for H. pylori infection in comparison to non - diabetic subjects. This association is found to be higher in those with long duration of DM and those with poor glycemic control.


Article
Association of Helicobacter Pylori Infection and Gastric Cancer

Author: Shurooq A. Lafta, Abdul Hussein M. ALFaisal
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 1 Pages: 1-9
Publisher: Baghdad University جامعة بغداد

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Abstract

Helicobacter pylori (H. pylori) is the most important etiologic factor for gastric cancer. It is one of the most common human pathogens, which colonizes in the mucus layer of the gastric epithelium in more than 50% of the population. The study include 78 samples of gastric cancer in addition to 42 blood samples. The results from 78 gastric cancer samples showed that gastric cancer can occur red at any age, but it increases in older ages and the incidence of H. pylori infected positive gastric cancer (HIP-GC) is higher than of H. pylori infected negative gastric cancer (HIN-GC). Male is the predominant in both groups and female affected by H. pylori more than male and the intestinal type was the predominant type.


Article
Evaluation of Anti-Helicobacter pylori IgG in Sera of Pregnant Women

Author: Samir Abdul Amir Abid Ali Alash
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2014 Volume: 17 Issue: 3 Pages: 108-110
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Helicobacter pylori is a bacterial infection of the stomach, which plays a major role in abdominal symptoms and gastroduodenal pathology. The pregnant women had a significantly higher relative risk of acquiring H.pylori infection during pregnancy as a result of physiological alterations. To investigate the relationship of H.pylori with dyspeptic symptoms in early and late pregnancy, thirty sera samples were obtained from pregnant women and thirty sera samples were obtained from apparently healthy women as control. All studied groups were evaluated anti-Helicobacter pylori IgG antibody by ELISA. Ten of pregnant women samples (33.3%) were seropositive of anti-H. pylori IgG antibody in pregnant women compared with control. This lead to suggest that H. pylori positive may be related to nausea and vomiting in pregnant women.

تعد بكتريا H.pylori احد انواع اصابات المعدة والتي تلعب دورا رئيسيا في اعراض البطن والامراض المعدية. تكتسب النساء الحوامل خطر الاصابة بهذه البكتريا كنتيجة الى التغيرات الفسيولوجية الحاصلة لديهم. لأجل التحري في مدى علاقة H.pylori مع اعراض عسر الهضم لدى النساء الحوامل في المراحل المبكرة والمتاخرة من الحمل، جمعت 30 عينة مصل دم من نساء حوامل و 30 عينة مصل دم من نساء غير حوامل سليمات ظاهرياً كمجموعة سيطرة، خضعت مجاميع الدراسة الى تقييم أضداد بكتيريا H. pylori من النوع IgG بأستخدام إختبار ELISA حيث أظهرت الدراسة أن 10 عينات من النساء الحوامل (33.3%) كانت مصولهن أيجابية لبكتيريا H. pylori. يمكن الأستنتاج من وجود أضداد بكتيريا H. pylori من النوع IgG في مصول النساء الحوامل ان له علاقة بأصابتهن بالغثيان والقيء.


Article
IN SILICO STUDY FOR PREDICTION OF DRUGTARGETS IN HELICOBACTER PYLORI
Helicobacter pylori دراسة حاسوب للتنبؤ بالأهداف الدوائية للبكتريا

Author: زينب هاتف عباس الركابي
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 4 Pages: 753-769
Publisher: Baghdad University جامعة بغداد

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Abstract

.ABSTRACT
In Silico Study, was conducted to search and predict essential biotargets which
might be as drug targets in Helicobacter pylori. Gene sequences (Protein sequences)
of the bacterium and its host (Homo sapiens) were retrieved from the Database for
Essential Genes (DEG) (http://tubic.tju.edu.cn/deg/).
The retrieved sequences were subjected to alignment process using BLASTP
2.2.23+(http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG
_DEF=blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seq ) with
different bioinformatic parameters and different matrices such as
PAM70,BLOSUM62 at different levels of E-values "0.001, 0.01, 0.05" to estimate
the essential bacterial genes that non-homologous with the human. Results showed
that 55 genes out of 323 essential bacterial genes have no homology with human
essential genes (118). The products of these genes (55 genes) were subjected to
programs to estimate the cellular location such as PSORTb v.3.0
(http://www.psort.org/psortb/index.html) and TMHMM
(http://www.cbs.dtu.dk/services/TMHMM/). The two programs showed that 22%
of non-homologous genes (12 protein) were associated with cell membrane, 24%
(13 protein) had unknown location, and the rest 54% (30 protein) were found in
the cytoplasm. It could be predicted that the membrane proteins might be good
targets for vaccine development and as well for antibiotics affected the cell
membrane.

الخلاصةللبحث عن الأهداف الحيوية التي يمكن أن تصلح أن In Silico تم إجراء دراسة على الحاسوبتم جمع تواليات الجينات الأساسية .Helicobacter pylori تكون أهدافاً دوائية في بكتريامن قاعدة المعلومات (Homo sapiens) (البروتينات الأساسية) للبكتريا ومضيفها الإنسانأُخضعت نواتج الجينات الأساسية .(http://tubic.tju.edu.cn/deg) للجينات الأساسيةBLASTP للبكتريا والإنسان لعمليات الإصطفاف بإستعمال+ 2.2.23http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=لتحديد . blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seqالبروتينات الأساسية غير المتشابهة للبكتريا مع بروتينات المضيف لتمثل أهدافاً دوائية وبإستعمال مؤشراتE-value وبقيم توقع مختلفة المستويات "PAM70,BLOSUM مختلفة من إذ المصفوفات " 620.05,0.01,0.001 ". أسفرت مجمل النتائج على إن هناك 55 جين من جينات البكتريا الأساسية " 323 " غير "متشابهة مع جينات الإنسان الأساسية " 118 ". أُخضعت البروتينات غير المتشابه لبرامج تحديد الموقع مثلTMHMM و (http://www.psort.org/psortb/index.html) PSORTb v.3.012 بروتين) هي بروتينات ) % وكانت النتائج 22 ،(/http://www.cbs.dtu.dk/services/TMHMM)30 بروتين) فكانت من ) % 13 بروتين) بروتينات غير محددة الموقع، أما الباقي 54 ) % غشائية و 24البروتينات السايتوبلازمية. يتوقع أن تكون البروتينات الغشائية أهدافاً جيدة سواء لإنتاج اللقاحات الوقائية أوالمضادات الحيوية التي تعمل على الأغشية. تهدف الدراسة محاولة إيجاد أهدافاً جديدة للأدوية نظراً لكونالبكتريا أصبحت مقاومة للمضادات والأدوية المستعملة في الوقت الحاضر

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