research centers


Search results: Found 2

Listing 1 - 2 of 2
Sort by

Article
Evaluation of Performance Characteristics of Commercially Available Tests for Diagnosing Hepatitis B Surface Antigenemia

Authors: Saad Hasan Mohammed Ali --- Saja Jehad Al-Khalidi --- Waleed Nadi Kasim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 159-171
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:A number of serologic immunoassays techniques have been developed in diagnostic virology with different degrees of sensitivity and specificity for the detecting hepatitis B virus (HBV) antigens and their relevant antibodies.OBJECTIVE:This study was designed to apply and assess the sensitivity and specificity of different commercially available laboratory techniques for detecting hepatitis B surface antigen(HBsAg).METHODS:One hundred and twenty-one sera samples were collected from National Center for Blood transfusion, Gastroenterology and Liver Diseases Hospital, Central Public Health Laboratories and Teaching Laboratories. According to the manufacturer’s practical instructions, a group of commercially available laboratory methods for detecting HBsAg were applied , including enzyme linked immunosorbent assay (ELISA), enzyme linked fluorescent assay(ELFA), immunochromatographic assay(ICA), and latex agglutination test(LAT).RESULT:Among ELISA, ELFA, ICA, and LAT methods for detecting HBsAg, the 3rd generation ELISA was proved to have very high specificity (no false negative results) and the least one that has necessitated few confirmatory repetitions. ELFA versus ELISA has showed relatively lower sensitivity (more false negative results). However, similar to ELISA, ELFA showed very high specificity. Immunochromatographic assay (ICA) confidentially appeared to be a good, rapid and simple technique with comparable sensitivity and specificity to ELISA and ELFA techniques. Although LAT was introduced as a rapid, simple and cheap technique for HBsAg screening, it showed frank lower sensitivity and specificity that deranged it from competing with all those tested techniques.CONCLUSION:The concomitant use of ELFA with ELISA compensates its relatively lower sensitivity in front of ELISA. Latex agglutination test for HBsAg has relatively lower sensitivity and specificity than all other tests. For its comparable performance characteristics to ELISA, the use of ICA is ideally suited for HBsAg screening, in respect to its lower cost, rapidity, simplicity and no need for expensive equipments


Article
Dry Blood Spots As Alternative To Conventional Serum Samples In Diagnosting Some Viral Infections
بقع الدم الجاف كبدیل لعینات المصل التقلیدیة في تشخیص بعض الاصابات الفایروسیة

Author: Abbas Arrak Abbas al-Tamimi عباس عراك عباس التمیمي
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2014 Volume: 10 Issue: 2 - part 2 Pages: 209-218
Publisher: Diyala University جامعة ديالى

Loading...
Loading...
Abstract

The purpose of this work was to validate the utility of dried blood spot ( DBS) samples for diagnosting HBV infection in comparison with the conventional serum samples.Pair-matched blood samples obtained from six HBsAg positive and four HBsAg negative persons by two ways; venipuncture and fingerstick by lancet and collect blood drops on filter papers (Zelpa type). Blood spots left to dry for two hours on flat bench then placed in nylon bag with dessicant to reduce humidity and stored in the refrigerator for one week.Before examination, blood spots were removed from filter papers and placed in tubes containing phosphate-buffered saline plus tween 80 and left overnight. ELISA for HBsAg screening was applied on sera and blood spot samples. Statistical analysis was done using pearson correlation test to test the optical densities of samples.statistical analysis showed no significant differences between serum and DBS samples and there is a strong linear correlation between the two types of samples.Dried blood spots can be used as successful alternative for serum samples in diagnosing certain viral diseases.

الهدف: تم اجراء هذا البحث لغر ض اختبار صلاحية عينات بقع الدم الجافة في تشخيص الاصابة بفايرس التهاب الكبد نمط بي بالمقارنة مع عينات المصل التقليدية. المواد وطرق العمل: تم اخذ عينات دم مزدوجة لستة مرضى موجبين لفحص HBsAg وأربعة سالبين للفحص للمقارنة وبطريقتين (طريقة السحب من الوريد وطريقة وخز الابهام وجمع قطرات الدم على اوراق ترشيح من نوع Zelpa). تركت الاوراق لتجف على سطح مستوي لمدة ساعتين. بعد ذلك وضعت اوراق الترشيح في كيس نايلون مع مادة مانعة للرطوبة desiccant وخزنت في الثلاجة لمدة اسبوع. قبل الفحص تم رفع المناطق الحاوية على قطرات الدم الجاف ووضعها في انابيب تحوي دارئ الفوسفات الملحي مع منظف tween 80 لغرض الفحص في اليوم التالي.أجري فحص الاليزا على عينات المصل والدم الجاف للكشف عن المستضد السطحي للفايرس HBsAg.النتائج: اظهرت النتائج عدم وجود فروق معنوية بين عينات الدم الجاف وعينات المصل وكذلك وجود علاقة خطية قوية بين قيم عينات المصل وعينات الدم الجاف.الاستنتاج: يمكن استخدام عينات الدم الجاف كبدائل ناجحة لعينات المصل ان لم تكن ذات اولوية في تشخيص بعض الاصابات الفايروسية.

Listing 1 - 2 of 2
Sort by
Narrow your search

Resource type

article (2)


Language

English (2)


Year
From To Submit

2014 (1)

2009 (1)