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Inhibitory effect of honey on some bacterial infections

Author: Najla'a Nabhan Yassein
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2011 Volume: 7 Issue: 3 Pages: 227-241
Publisher: Diyala University جامعة ديالى


The antibacterial profiles of honey were examined against clinical isolates of
Staphyllococcus aureus and Pseudomonas aeruginosa. Agar well diffusion method was used
in the antibacterial susceptibility studies. This study revealed that the honey obtained from
Agricultural college of Baghdad University was effective against bacteria.The highest
inhibitory zone of S. aureus reached to 20mm in diameter at 200 mg/ml of honey
concentration but not affected on P. aeruginosa. Wounds were done in male mice by using
shaver and then infected with 0.1ml of both bacterial suspensions with concentration 108
CFU/ml.Symptomes of infections were appeared after 24-48 hours. Three concentrations of
honey were used (100,200 and 300) mg/ml for treatment of skin infections by using 0.1 ml of
honey,also gentamycin ointment and normal saline were used. Symptomes of recovery based
on wound healing were noticed after 10,6,9,11 and 15 days for S. aureus and 12,8,13,15 and
18 days for P. aeruginosa when treated with 100,200 and 300mg/ml of honey,gentamycin and
normal saline respectively. 200 mg/ml of honey concentration showed the best concentration
for skin infection treatment. This study,therefore, suggest that honey could have strong
biocidal effect against S. aureus (both in vitro and in vivo) and against P. aeruginosa (in
vivo),therefore,have the potential effective role in the treatment of skin infection.

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