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INDIRECT IMMUNOFLUORESCENCE EXPRESSION OF ZO-1 AND PAN-CADHERIN DURING MATURATION OF HEPATOCYTES AND BILIARY NETWORK FORMATION IN MICE

Authors: Noor F.M. Ali نور فؤاد محمد علي --- Hayder J. Mubarak حيدر جواد مبارك
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 3 Pages: 242-249
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: The tight junctional complexes had been demonstrated in hepatocytes and in both epithelium and endothelium. In multicellular organisms, cell-cell adhesion is critical for development and morphogenesis.Objective: To investigate the spatiotemporal organization of the immunohistochemical markers anti ZO-1 and anti Pan-cadherin during the prenatal development of the mice liver and to correlate the expression of these markers with histogenesis during hepatocyte maturation and biliary network formation.Methods: Forty eight (48) pregnant mice were scarified at subsequent gestational days from day 14 till the first day of postnatal life. Paraffin blocks and sections of the liver extracted from the embryos obtained from these pregnant mice and from neonates at the first postnatal day of life were prepared. The sections were stained using the anti ZO-1 and anti Pan-cadherin immunohistochemical markers.Results: The sequential steps of anti ZO-1 indirect immunofluorescence reactivity in the developing liver tissue showed chronological variability, during days 14, 15, and 16; positive cell surface labeling was weaker adjacent to the blood vessels. The next step includes anti ZO-1 reactivity in late prenatal and postnatal liver tissue showing marked uniform reactivity. The sequential steps were also demonstrated in the anti Pan-cadherin immunohistochemical reactivity. The reactivity of the liver tissue during days 14, 15, and 16 showed disregarded cell surface labeling that increased markedly in later prenatal and postnatal periods.Conclusion: The initial stages in the development include the undifferentiated specification the hepatic lineage characterized by regional establishment of zonula occludens in the developing liver cells and paucity in the development of the zona adherens complexes. The later stages showed histological and functional maturation reflected by anti ZO-1 and anti Pan-cadherin reactivity that represent a requirement for the physiological functions in the entire liver tissue.Keywords: Embryo, mice, Zo-1, Pan-cadherin, immunhistochemistry. Citation: Ali NFM, Mubarak HJ. Indirect immunofluorescence expression of ZO-1 and Pan-cadherin during maturation of hepatocytes and biliary network formation in mice. Iraqi JMS. 2017; Vol. 15(3): 242-249. doi: 10.22578/IJMS.15.3.5

Keywords

Embryo --- mice --- Zo-1 --- Pan-cadherin --- immunhistochemistry


Article
13.HISTOMORPHOMETRIC AND ENDOTHELIN-1 IMMUNOHISTOCHEMICAL STUDY OF HUMAN PLACENTAL VILLI CORRELATED WITH UMBILICAL CORD COILING INDEX

Authors: Hayder J. Mubarak حيدر جواد مبارك --- Sarah A. Ahmed سارة عبد الحسين احمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 2 Pages: 198-205
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Umbilical cord coiling index plays a role in predicting the pregnancy outcome and risk of low birth weight. Endothelin-1 binding sites exist on placental stem villi vessels and trophoblastic layer of the villi. Endothelin-1 is involved in regulation of the feto-placental circulation and specific trophoblastic functions.Objective: To investigate the profile of anti-endothelin-1 antibody expression in the human normal placental villi in relation to the coiling indices of the umbilical cords attached to these placentas.Methods: Normal human placentas were collected with inclusion criteria (full term newborns with normal perinatal outcome whose mothers were normal), and classified according to their umbilical cord coiling index into three groups: (N, H, and H), endothelin-1 marker used to investigate the localization of the endothelin-1 in the placental villi of each group.Results: There was a difference between the mean positivity percentage of endothelin-1 immunohistochemical reactivity in normocoiled group in comparison with hypercoiled group and hypocoiled group. There is a difference between the mean number of terminal villi in the three groups and in the perimeter of blood vessels.Conclusion: The pattern of endothelin-1 reactivity is associated with vasodilatation of the villous vascular bed to maximize the exchange function of the placenta as a physiological response to overcome the sequel of obstruction of umbilical vessels in both types of abnormal coiled cords during pregnancy.Keywords: Placental villi, umbilical cord, coiling index, normal pregnancy, endothelin-1, immunhistochemistry.Citation: Hayder J. Mubarak, Sarah A. Ahmed. Histomorphometric and endothelin-1 immunohistochemical study of human placental villi correlated with umbilical cord coiling index. Iraqi JMS. 2017; Vol. 15(2): 198-205. doi: 10.22578/IJMS.15.2.13

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