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Article
DETECTION OF BACILLUS THURINGIENSIS CRYSTAL PROTEIN CRY51AA1 INTOXICATION (IN VIVO) USING ZEBRAFISH MODEL

Author: Ali B.T. Aldeewan
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2017 Volume: 16 Issue: 2 Pages: 26-35
Publisher: Basrah University جامعة البصرة

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Abstract

Bacillus thuringiensis is a ubiquitous a gram-positive, anaerobic, spore-formingbacterium, produces various proteins (toxins) during the stationary and vegetative phaseof its growth cycle. Some of these proteins belonging to this group showed weaksimilarities to ß-barrel pore-forming toxic proteins, such as the cytotoxins ofPseudomonas aeruginosa, the epsilon toxin of Clostridium perfringens, alpha-toxin ofClostridium septicum. In the present study, the intoxication effects of Cry51Aa1 proteinproduced by B.thuringiensis F14-1 strain was evaluated in vivo, in order to investigate theability of activated Cry51Aa1 to make a change in size or functions of zebrafishcardiovascular system and induces toxicity in adult zebrafish. Using in vivo imaging weobserved that Cry51Aa1 has no scientific effect on cardiac function and development ofzebra fish embryo or have toxic effect on adult in spite of use high concentration, so it issafe to use and a potentially effective agent in breast cancer therapy.


Article
The in vivo effect of some medicinal plant extracts on Cryptosporidium parasite.
تاثير بعض مستخلصات النباتات الطبية على طفيلي داء البويغات الخبيئة في الحي.

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Abstract

Cryptosporidium is known as a parasite of humans especially children of both those who are immunodeficient and immunocompetent. The effect of prolonged diarrhea and dehydration can be dangerous, especially for young and immunodeficient persons. This study was designed to find out the watery and alcoholic effect of some medicinal plant extracts against the parasite. No significant differences between the watery and alcoholic extracts of the three medicinal plant used (Corindrum sativum, Curcuma longa, Viscum album) were noted. All the plants were dose related, Curcuma longa had the highest effect on Cryptosporidium oocysts shedding in laboratory infected Balb/c mice. With rate of 100% on the 7th day of treatment at 750 mg/kg and on the 5th day at 1000mg/kg in the watery extracts. And a rate of 100% on the 4th day at 1000mg/kg in alcoholic extracts, followed by Viscum album with rate of 48, 54% on the 7th day at 750, 1000mg/kg respectively for watery extract and 73, 76% on the 7th day at 750, 1000mg/kg respectively for alcoholic extract. The Coriondrum sativum had the lowest effect at all concentrations used in both watery and alcoholic extracts. No significant differences were seen with folic acid and potassium chloride adding to the watery and alcoholic plant extracts. Except with the antibiotic (azithromycin) in which its activity was increased to 100% on the 4th day of treatment whereas its effect was only 68% without them

يعرف داء البويغات الخبيئة بانه طفيلي يصيب الانسان و خاصة الاطفال من كلا الفئتين ذوي المناعة الطبيعية و ذوي المناعة القليلة . تأثير الاسهال المتسبب عن الطفيلي و فقدان السوائل الجسمية اثناء الاصابة قد يكون خطيرا جدا خاصة في الفئات العمرية الصغيرة و الاشخاص ذوي المناعة القليلة . صممت هذه الدراسة كمحاولة لأيجاد علاج للداء باستخدام المستخلصات الكحولية و المائية لبعض النباتات الطبية . لم يكن هناك فروق معنوية بين المستخلصات الكحولية والمائية للنباتات الثلاثة المستخدمة ( الكركم ، الكزبرة ، الدبق ), وكل المستخلصات المستخدمة كانت ذو علاقة طردية مع التركيز حيث ازدادت تاثيراتها بزيادة التراكيز . كان لنبات الكركم التاثير الاقوى لتقليل معدل الاكياس المطروحة في براز الفئران المعالجة نوع Balb/c, بمعدل تاثير 100٪ في اليوم السابع من العلاج عند تركيز 750 ملغم/كغم و اليوم الرابع عند تركيز 1000 ملغم/كلغم في المستخلص المائي و معدل 100٪ في اليوم الرابع من العلاج عند تركيز 1000 ملغم/كغم مع المستخلص الكحولي. الدبق كانت في المرتبة الثانية بمعدل تاثير54,48٪ في اليوم السابع عند تركيز 750, 1000 ملغم/كلغم على التوالي للمستخلص المائي, ومعدل تاثير 76,73 ٪ في اليوم السابع لنفس التركيزين السابقين على التوالي للمستخلص الكحولي. نبات الكزبرة كانت لها التاثير الاقل عند كل التراكيز المستخدمة ومع كلا المستخلصين الكحولي والمائي. لم يلاحظ فروق معنوية عند اضافة حامض الفوليك او كلوريد البوتاسيوم معا او بصورة منفصلة الى المستخلصات النباتية ماعدا عند اضافتهما معا الى المضاد الحيوي ازثرومايسين حيث سببا زيادة في تاثيره الى نسبة 100٪ في اليوم الرابع من العلاج بعد ان كان نسبة تاثيره 68٪ فقط دون المادتين .


Article
Stability of Resistance Induced by Escherichia coli in Comparison with That Carried by Clinical Isolates in Vivo

Authors: Lubna A. Kafi --- Sajaa R. kareem Al-Saedi --- Ali A. Al-Khayyat
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2013 Volume: 6 Issue: 1 Pages: 1546-1551
Publisher: Kerbala University جامعة كربلاء

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Abstract

ackground: The increasing incidence of resistance to a wide range of antibiotics bymicroorganisms is a major concern facing modern medicine because these antibioticsare widely used in human and veterinary medicine to treat and prevent diseases and asgrowth promoters in animal intensive industries. The consequences are severe. Infectionscaused by resistant microbes fail to respond to treatment, resulting in prolonged illness andgreater risk of death.Objectives: The aims of this study are: 1-Comparing the changes of induced resistance inE.coli with that present in clinical isolates of the same microorganism. 2-Determine theproperties of cefquinome especially the development of resistance and the stability of itMethods: fourteen strains of E.coli were collected from different disease cases: diarrhea(children= 6, calve= 3, poultry= 1), UTI (urine= 2), mastitis (milk= 2) from Al-Yarmookhospital and farms of veterinary college (Baghdad). The MIC was estimated eight selectedstrains (4 sensitive and 4 resistances) before and after passages these strains in vivo forcarried out the comparison.Results: A significant drop in induced resistance for sensitive strains as compared withclinical isolates which are not significant elevate in resistance. Most E.coli strains showedhighly susceptible to cefquinome but some of them were appeared resistance although thisantibacterial was newly used in this country.Conclusion: loss of induced resistance when passing in vivo because the resistantmicroorganisms actually were unstable group when comparison with stable resistance inclinical isolates was not changed when exposed to the same condition.Keywords: E. coli, MIC, in vivo, antibacterial.

Keywords

E. coli --- MIC --- in vivo --- antibacterial.


Article
Dimensional accuracy of impression techniques for the endosteal implants (An in vivo study): Part II

Authors: Nadira A Hatim --- Basim M Al–Mashaiky
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2007 Volume: 7 Issue: 2 Pages: 131-137
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To determine the most accurate impression techniques and materials to transfer the exactposition of a single implant to stone die to construct the prosthesis for selected clinical cases. Materialsand methods: Five clinical cases were selected with special criteria to construct implant (Frialit–2)prosthesis on a stone die with high accuracy according to the results of study. Four impressiontechniques were used (direct and indirect, each with one and two steps) using condensation, addition(heavy, medium and light consistencies) silicone impression materials. Ten impressions were taken foreach patient to produce a total number of 50 stone casts. The direct and indirect measurements wereperformed by using digital vernier. Results: The significant differences between the impressiontechniques at the three axes of dimensions (p ≤0.05) were applied clinically to construct the fixedprosthesis for each patient. The results of this study showed that the high dimensional change oflaboratory cast was related to indirect one step impression technique to transform the implant positionfrom patient mouth to laboratory cast. Addition curing (medium consistency) silicone impressionmaterial produced the lowest accurate stone die. Conclusions: The results of this study showed that thedirect two steps impression technique was the accurate impression technique for transformation ofimplant position from patient mouth to laboratory cast. Additional curing (light and heavy consistency)silicone impression material produced the most accurate stone die and gave a successful treatment tothe patient.


Article
IN VITRO AND IN VIVO STUDY: BY USING OF He-Ne LASER AS PROTOSCOLICIDAL AGENT OF ECHINOCOCCUS GRANULOSUS

Authors: Suha Adel Qasim --- Dhilal Mahdi Al-Muathen
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2009 Volume: 12 Issue: 4 Pages: 123-126
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Hydatid protoscolices that obtained from sheep infected with Echinococcus granulosus, were irradiated in culture medium in vitro with He-Ne Laser (632.8 nm, 0.5 mW) for different periods (0, 5, 15, 30, 60, and 120) min, and energy densities (0, 1.19, 3.58, 7.17, 14.33, and 28.66) J/cm2. The percentage of the protoscolices viability was assessed after the incubation at temperature 20oC and pH 7.4 at the time intervals (1 hour, 1 day, 4 days, 6 days, and 12 days) after each irradiation period. It was seen that over the time intervals up to 4 days, the irradiation periods (60 and 120) min, and the energy densities (14.33 and 28.66) J/cm2 reduced the viability in irradiated groups as compared with zero time which was considered as control, whilst all the irradiation periods had no reliable effect at the incubation interval 1 hour. In vivo the results also revealed a noted reduction in the cysts numbers and their diameters at different irradiation periods, and no cysts were found in albino mice incubated with culture irradiated for 120 min.

تم الحصول على نماذج الأكياس العدرية من الخراف المصابة بطفيلي Echinococcus granulosus من المجازر المحلية وشععت الرؤيسات الأولية بعد تهيئتها في الزجاج بليزر الهليوم-نيون (0.5 ملي واط)، (8,632 نانوميتر) للفترات الزمنية (0، 5، 15، 30، 60، و 120) دقيقة وكثافة طاقة (0، 1.19، 3.58، 7.17، 14.33 و 28.66) جولسم2. وتم حساب النسبة المئوية لحيوية الرؤيسات الأولية للطفيلي بعد الحضن بدرجة حرارة 20 مo، وحامضية 7.4لفترات زمنية (ساعة، يوم، 4 يوم، 6 يوم، و12 يوم) بعد كل فترة تشعيع، وقد لوحظ أن الفترات الزمنية فوق 4 أيام حضن لفترتي التشعيع (60 و 120) دقيقة و كثافة طاقة (14.33 و28.) جولسم2 أظهرت أختزالاً واضحاً في النسبة المئوية لحيوية الرؤيسات مقارنة مع زمن التشعيع صفر الذي أعتبر كمجموعة سيطرة، في حين كانت نتائج فترة الحضن 1 ساعة ليست ذات تأثير يعتمد عليه لجميع فترات التشعيع. وقد كشفت النتائج أيضاً أختزالاً واضحاً في عدد الأكياس العدرية وأقطارها في الحي، كما لم يعثر على أي كيس عدري في الفئران البيض المحقونة بمزروع الرؤيسات الأولية المشععة لمدة 120 دقيقة.


Article
Effect of Lactobacillus acidophilus on Escherichia coli causing Urinary tract infections in Vitro and in Vivo
تأثير جرثومة الحليب الحامضية Lactobacillus acidophilus في الايشريشية القولونية E. coli المسببة لخمج المجاري البولية داخل و خارج الجسم الحي

Authors: Alaa Muyassar Ahmed الاء ميسر احمد --- Harith J. Fahad حارث جبار فهد
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN: 19918941 Year: 2011 Volume: 5 Issue: 1 Pages: 1-6
Publisher: University of Anbar جامعة الانبار

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Abstract

One hundred and sixty five mid stream urine specimens were collected from outpatients presented with urinary tract infections (UTI). The results showed the dominance of Escherichia coli over other causative agents. Antibiotic sensitivity test was carried out to E. coli isolates. Thence, the isolate that developed the highest multidrug resistance was chosen for further studies. Moreover, five Lactobacillus isolates comprising L. acidophilus L1 and L. acidophilus L2 were isolated from yogurt and vinegar, respectively, L. plantarum L3 and L. plantarum L5 from saliva and raw milk, respectively, while L. fermentum L4 was isolated from vagina. Cup assay method was employed to investigate the inhibitory (antagonistic) activity of lactobacilli isolates against E. coli A99 on MRS agar. Results showed that L. acidophilus L1 developed the highest activity. The cell free supernatant of lactobacilli developed the same activity. L. acidophilus L1 supernatant showed the highest inhibition activity. The present study also revealed this activity in vivo by injecting a group of mice with L. acidophilus L1 suspension or its infiltrate 30 min after injecting the E. coli A99 intraurethrally and the histopathological sections revealed the disappearance of inflammation signs caused by E. coli A99 when it was injected alone.

جمعت ١٦٥ عینة اد ا رر وسط المجرى من مرضى مصابین بخمج المجاري البولیة. اذ اظهرت نتائج الد ا رسة الحالیة سیادةعلى بقیة المسببات المرضیة. درست الحساسیة للمضادات الحیاتیة لهذه الج ا رثیم و انتخبت العزلة الاكثر مقاومة Escherichia coliL. acidophilus L و 2 L. acidophilus L مثلت 1 Lactobacillus لد ا رسات لاحقة. كما عزل خمس عزلات من جرثومةالمعزولة من اللعاب و الحلیب الخام على L. plantarum L و 4 L. plantarum L المعزولة من اللبن و الخل على التوالي و 5من المهبل. استعملت طریقة كأس الاغار للتحري عن الفعالیة التثبیطیة لعزلات L. fermentum L التوالي في حین عزلت 4اظهرت اعلى L. acidophilus L اذ بینت النتائج ان 1 MRS على الوسط الزرعي E. coli A ضد العزلة 99 LactobacillusL. acidophilus L فعالیة تثبیطیة مشابهه في حین اظهر طافي 1 Lactobacillus فعالیة. علاوة على ذلك اظهر طافي خلایاL. الفعالیة الاكبر. كما بحثت الد ا رسة الحالیة هذه الفعالیة داخل الجسم الحي عن طریق حقن مجموعة من الفئ ا رن بعالق خلایاعن طریق الاحلیل واخذت الكلى و المثانة للد ا رسة E. coli A او طافي تلك الخلایا بعد ٣٠ دقیقة من حقن 99 acidophilus L1عندما حقنت لوحدها. E. coli A النسیجیة التي بینت اختفاء علامات التهاب التي تسببت بها 99EDBAG


Article
Brackets bonding failure after in-office bleaching in vivo

Authors: Dr.Amer Al-Obiedy, B.D.S., M.Sc --- Dr. Ahmed S. Mustafa B.D.S, M.Sc د.بان غانم --- Dr. Bahn Gh. Agha B.D.S, M.Sc
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2010 Volume: 7 Issue: 1 Pages: 49-56
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The aim of this in vivo study was to determine the effect of high concentration35% hydrogen peroxide bleaching agent on metallic brackets bonding failure. Fortypatients who received first orthodontic treatment phase, were included in this study.A split mouth technique was used; one arch exposed to in-office bleaching gelcontaining 35% hydrogen peroxide for 30 minutes, while the unbleached arch servedas the control. Patients were divided into four groups: First two groups, the bracketsbonded 48 hours after bleaching while the third and fourth groups, brackets bonded 3weeks after bleaching. The bracket failure was computed using independent T-test.The higher bracket failure occurs in mandibular bleached group 48 hours beforebonding (18.33%) while the lowest bracket failure occurs in unbleached groups (1.67%). Significant differences were showed in bracket failure between the mandibularbleached teeth 3 weeks before bonding and those 48 hours before bonding. AdhesiveRemnant Index scores revealed that the majority of failure in bleached teeth occurredin the enamel/resin interface. The 48 hours bleached teeth before bondingsignificantly had a high bracket failure. We should increase the time lapse betweenbleaching and bonding procedure more than 3 weeks to improve the bracket bondingstrength.


Article
Callus induction from Nerium oleander leaves and qualitative determination of some active compounds in vivo and in vitro
أستحثاث الكالس من أوراق نبات الدفلة Nerium oleander وتقدير بعض مركباته الفعالة نوعياً داخل وخارج الجسم الحي

Author: Liqa,a Ali Jasaa لقاء علي جازع
Journal: Jornal of Biotechnology Research Center مجلة مركز بحوث التقنيات الاحيائية ISSN: 18151140 Year: 2013 Volume: 7 Issue: 3 Pages: 12-17
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Callus induction of Nerium oleander young leaves explants was studied using Murashige & Skooge (MS) Medium supplemented with 2,4-dichlorophenoxy acetic acid( 2,4-D 0.0, 0.5, 1.0, 2.0) mg/l with Benzyle adinine (BA0.0, 0.5 or 1.0, mg/l). comparative study was made for qualitative determination of some active compounds between the initiated callus and leaves of fieled plant (intact plant). Results showed that 2,4-D at 1.0 or 2.0 mg/l with BA at 0.5 or 1.0, mg/l were the best for callus induction that gave the highest fresh and dry weight. Qualitative detection showed that (Alkaloids, Flavonoids, Terpenoids, Tanins, Saponins, and Cardiac Glycosides) the main active compounds were found in callus and leaves of field-grown plant.

دُرست أمكانية أستحثاث الكالس من زراعة قطع الأوراق الفتية لنبات الدفلة Nerium oleander بأستعمال وسط (MS) Murashige & Skooge وبأضافة منظمات النمو النباتية (2,4-D) 2,4-dichlorophenoxy acetic acid بالتراكيز (0.0، 0.5، 1.0، 2.0) ملغم/لتر و Benzyle adinine (BA) بالتراكيز (0.0، 0.5، ،1.0) ملغم/لتر. أجريت دراسة مقارنة لوجود بعض المركبات الفعالة الرئيسة بين الكالس المنتج و أوراق النبات الحقلي (النبات الأم) بأستعمال عدد من الكواشف النوعية. بينت النتائج أن أضافة 2,4-D بتركيز 1.0و 2.0 ملغم/لتر و BA بنركيز 0.5و 1.0 ملغم/لتر للوسط والتداخل بينهما الأفضل لتحفيز نشؤ الكالس وأعطاء أعلى قيم للأوزان الطرية والجافة، وأوضحت نتائج الكشوفات النوعية أن المحتوى الداخلي للكالس المتحفز لم يختلف عن أوراق النبات الحقلي (النبات الأم) من ناحية وجود المركبات الفعالة الرئيسة (Alkaloids, Flavonoids, Terpenoids, Tanins, Saponins, Cardiac Glycosides).


Article
Evaluation of the Effect of Implant-numbers, Position and Complete Over Denture on Alveolar Bone Resorption

Authors: Ali M. AlSheakh --- Nadira A Hatim
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 80-92
Publisher: Mosul University جامعة الموصل

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Abstract

The aims: The aims of this study were to evaluate the alveolar bone resorption every 2.5mm distal to the implant over denture to the retromolar pad in the mandibular arch, and to the maxillary tuberosity in the maxillary arch in edentulous patient according to number between two and four, and position of implants between canine and 2nd premolar. Materials and Methods: Eight patients (age range 45-60 years) were selected from the department of Prosthodontics/ College of Dentistry/Mosul University, who had at least one edentulous arch. All patients complaining from poorly retention conventional acrylic complete denture due to the residual ridge resoption, but the alveolar ridge height need at least 10mm implant length.After completing the surgical steps of 26 implants (two or four implant screw type titanium), over denture type with one step surgery for all implant types were constructed in conventional method after one month healing period. The prosthesis was delivered to the patients after one months of making the period elapsed. Dentures were delivered without socket attachment(six months). For assessment of alveolar bone height, for each patient panoramic exposure of OPG was recorded – three times [base line(at time of placement) , 6 months and 12 months]. Results: Results of this study showed, that mean difference of bone resoption range between [-0.2 –(-0.6)mm], and there was a significant difference of bone resoption between base line and 12 months to p0.05 according to number and position of implants. Conclusions: The conclusion of this study showed that, there was no significant different of alveolar ridge bone resoption between implants number in two or four implants over denture or implant position


Article
Preparation, Characterization and Evaluation of Some Metronidazole Formulations as Films and The study of Their Medicinal Activities Against Pathogenetic Microorganisms Causing Dermatitides.
تحضير بعض صيغ المترونيديزول كأفلام وتقييمها ودراسة فعالياتها الدوائية ضد الاحياء المجهرية المرضية المسببة للألتهابات الجلدية.

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Abstract

In the present study, a labor has been made to develop film type of transdermal therapeutic system comprising different concentrations of metronidazole with hydrophilic polymeric combinations using solvent casting (evaporation) technique. The DSC analysis confirmed that ester bonds were formed between the used polymers in preparing the films. The prepared films were considred semi-IPNs hydrogels and the mixture between the used polymers and metronidazole in preparing the films is considred physical mixing viz., chemical, physical and therapeutical properties of drug remained themselves. Two main parts including chemical and biochemical were discussed in this study. In the chemical part, the prepared films F0 and F3 were evaluated via the study of weight and thickness uniformities and swelling behaviour. In vitro drug release and kinetics of drug release were studied for all the prepared pharmaceutical formulations in this study. F3 was higher average weight and thickness as compared to F0 and in addition to their possession of the same swelling behaviour which was explained on the basis of the increase in swelling index up to period at 70 min and which was followed by the decrease in this index according to cleavage of the formed ester bonds in the crosslinked structure, erosion process with physical disintegration and mass loss. Also, the prepared formulation F3 followed zero order, Korsmeyer-Peppas model and Fickian (n<0.5) transport mechanism (diffusion controlled release). In the biochemical part, the biochemical activities of all the prepared formulations (as antimicrobials) were studied (in vitro) in four types of microbes including Staphylococcus aureus (Gram-positive), Staphylococcus epidermidis (Gram-positive), Candida albicans (yeast) and Trichophyton sp. (mold) by using agar diffusion method. These formulations showed increasing in their biochemical activities against these isolates and these activities associated with the increase in drug concentrations which were loaded with the selected polymers in this study and in addition to their possession of highest antimicrobial and biochemical activities to inhibit the growth of these microbial isolates as compared to pure drug and commercial gel at (p<0.05). Also, the prepared formulations including F0 and F3 were applied (in vivo) to skin of male rabbits for studying subacute toxicity test (LD50) and skin (back and rectal) irritation tests. These formulations were not poisonous and non irritant.

في الدراسة الحالية, جرى العمل لتطوير أنواع من الهلاميات لنظام علاجي عبر الجلد مؤلفا من تراكيزمختلفة من المترونيديزول مع أتحادات بوليمرية آلفه (محبه) للماء بأستخدام تقنية تبخر المذيب. لقد أثبت تحليل المسح الحراري التفاضلي (DSC) تكون أواصر أسترية بين البوليمرات المستخدمة في تحضير الافلام, وأعتبرت الافلام المحضرة هلاميات من نوع أشباه بوليمرات شبكية التداخل والتي تعرف أيضا بأسم السبائك البوليمرية (semi-IPNs), كما وأعتبر مزيج البوليمرات والدواء المستخدمين في تحضير الافلام مزيجا فيزيائيا, أي إن الخصائص الفيزيائية والكيميائية والعلاجية للدواء بقيت نفسها دون أن تتغير..لقد تضمنت هذه الدراسة جانبين رئيسيين تمثلا بالجانب الكيميائي والجانب الكيموحياتي .ففي الجانب الكيميائي تم تقييم الافلام المحضرة F0 و F3من خلال دراسة اتساق (انتظام) الوزن, واتساق السمك, وسلوك الانتفاخ. كما وتمت دراسة سرع تحرر الدواء وحركيات سرع تحرر الدواء (خارج الجسم الحي) وذلك لكل الصيغ الدوائية المحضرة. ولقد تبين بأن الصيغة المحضرة F3 كانت أعلى من حيث معدل الوزن ومن حيث السمك مقارنة مع الصيغة المحضرة F0, وبالاضافة إلى إمتلاك تلك الصيغتين لنفس سلوك الانتفاخ والذي فسر على أساس الزيادة في نسبة الانتفاخ لغاية الفترة الزمنية عند 70 دقيقة والتي أتبعت بنقصان في تلك النسبة وفقا لكسر الاواصر الاسترية المتكونة في التركيب المتشابك, عملية تأكل مع تحطم فيزيائي, وفقدان كتلة. كما وتبين بأن الصيغة المحضرة F3 أتبعت المرتبة الصفر, نموذج (Korsmeyer-Peppas), وميكانيكية الانتشار المسيطر (Fickian (n<0.5) transport) . أما في الجانب الكيموحياتي فقد تم دراسة الفعاليات الكيموحياتية (خارج الجسم الحي) لكل الصيغ المحضرة (كمضادات الجرثومية) وذلك لأربع عزلات جرثومية أشتملت Staphylococcus aureus (الموجبة لصبغة كرام), Staphylococcus epidermidis (الموجبة لصبغة كرام) , Candida albicans (خميرة), و Trichophyton sp.(عفان) بطريقة الأنتشارالمتبعة بالحفر. وقد تبين بأن إزدياد الفعاليات الكيموحياتية لهذه الصيغ تجاه تلك العزلات قد ترافق مع الزيادة في تراكيز الدواء المحمل مع البوليمرات المختارة في تلك الدراسة, وبالاضافة إلى إمتلاك تلك الصيغ لفعاليات كيموحياتية وضد جرثومية عالية لتثبيط نمو العزلات الجرثومية وذلك بالمقارنة مع الدواء لوحده ومع الهلام التجاري عند مستوى إحتمالية أقل من 05,0(p<0.05). كما تم تطبيق الصيغتين المحضرتين F0 و F3 على جلد أرانب ذكور لدراسة السمية (subacute toxicity (LD50)), إختباري إثارة (تهيج) الجلد, وإختبار معالجة إثارة (تهيج) الجلد المستحدث. وقد تبين بأن تلك الصيغ لم تكن سامة أو مثيرة (مهيجة) للجلد.

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