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Article
Detection of Human Cytomegalovirus in Colorectal Adenocarcinoma by In Situ Hybridization Technique

Author: Faiza Abdulla Mukhlis
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2009 Volume: 51 Issue: 1 Pages: 52-56
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human Cytomegalovirus (HCMV) infects a wide range of human cells, including colonic epithelial cells that give rise to adenomas and adenocarcinomas. Persistent productive infection of tumor cells is essential for oncomodulation by HCMV.This study aimed to detect HCMV matrix protein using in situ hybridization technique (ISH) in colorectal adenocarcinoma compared to normal colon tissues, and to the presence of cytomegalovirus inclusion bodies in infected colorectal carcinomas.Patients and methods: Twenty six of colorectal adenocarcinomas were obtained in paraffin-blocks compared to 10 normal colon specimens which were age and sex matched as control group. Detection of HCMV was obtained by in situ hybridization technique.Results:The biotinylated probe specific for DNA encoded HCMV-matrix protein showed hybridization with nucleic acid in 20 cases out of (26) of colorectal adenocarcinomas representing (76.9%) compared to normal colon tissue which revealed no hybridization signals. Moderate to high scoring signals were detected in moderate to poorly differentiated groups. Inclusion bodies were detected in 11 (42.3%) cases with positive hybridization signals.Conclusion: HCMV may play a role in the colorectal adenocarcinoma pathogenesis. In situ hybridization test are considered the most sensitive and specific tools for detection of HCMV DNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies and molecular studies are necessary to confirm the association of HCMV and colorectal tumorogenesis in Iraqi population.


Article
Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique

Authors: Ban A. Abdul majeed** PhD --- Faiza A. Mukhlis* PhD --- Shatha F. Abdullah* FICM
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2009 Volume: 51 Issue: 2 Pages: 178-183
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can transform cells and dysregulate many cellular pathways involved in oncogenesis. This study was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion bodies in brain tumor tissues.Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks compared to eight normal brain autopsy specimens which were age and sex matched with the study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens representing (73.9% ) of the total study groups . All cases with high grades astrocytoma revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 ( 21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma cases(5.3 %), revealed inclusion bodies on histological examination. Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular studies are necessary to confirm the association of HCMV related human cancers in general Iraqi population.


Article
دراسه نسجيه ومناعيه لمرض المليساء المعديه Molluscum contagiosum في العينات المعزوله من مستشفى مرجان التعليمي

Authors: نسرين كاظم راضي --- رفاه هادي لطيف --- محمد كاظم الحطاب
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2016 Volume: 24 Issue: 6 Pages: 1522-1529
Publisher: Babylon University جامعة بابل

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Abstract

Thirty samples of serum and skin tissue were collected from patient infected with Molluscum contaginosum from Marjan hospital. The histological examination of skin tissue was done by using light microscope, serum was collected from patients for detection of Fasligand immunological protein the result shows that : the histological study was immportant for the dignosis of MCV disease due to the presence of evidant dignostic featuer (molloscum bodies). The highest rate of infection occurs at age group >20y was 40% with Mean concentration of FSAL about 94.25±30.40 ,the infection rate was 66.7% for male with concentration for FSAL (82.85± 18.10),The study show also infection rate for marriad patiant was 36%with concentration of FSAL (90.18± 30.56 ) and 70% of infection rate were in urban aerea 30%in rular aerea.

جمعت 30 عينه من مستشفى مرجان التعليمي لمقاطع نسيجيه للجلد ونماذج المصل من الاشخاص المصابين بمرض المليساء المعديه Molluscum contagiosum وفحصت المقاطع النسجيه بالمجهر الضوئي المركب .تم فحص عينات المصل باستخدام الانزيمات المرتبطه مناعيا Elisa kit) ) لقياس البروتين المناعي( Fas Ligand (FasL .بينت النتائج اهميه الدراسه النسجيه في تشخيص المرض بسبب وجود الاجسام الاشتماليه للفيروس في المقاطع النسجيه المثبته على الشرائح الزجاجيه ,وجد ان اعلى نسبه للاصابه كانت 40% للفئه العمريه الأقل من 20سنه اذ بلغ معدل تركيز ال Fasligandلهذه المجموعه(94.25±30.40) ,وان نسبه الاصابه .766%للذكورمع معدل تركيزللFasligand 82.85± 18.10)) ونسبه اصابه المتزوجين 36%مع معدل تركيز Fasligand (90.18± 30.56) ,ان نسبه الاصابه في المناطق المتحضره 70% اما في المناطق الريفيه فبلغت 30%.

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